scholarly journals On the histology of Uredo dispersa , Erikss., and the 'mycoplasm’ hypothesis

1903 ◽  
Vol 71 (467-476) ◽  
pp. 353-354
Keyword(s):  
Microscopic Examination ◽  
Histological Features ◽  
Bromus Secalinus ◽  
Staining Methods

The paper deals with a detailed study of the histological features of the germination, infection, and growth of the mycelium of the Uredo in the tissue of grasses. Primarily, the figures refer especially to the Uredo of Puccinia dispersa in the tissues of Bromus secalinus , but comparisons are made with the behaviour of this and other Uredineæ— e. g ., Puccinia glumarum and P. gramins —in the tissues of other grasses and cereals. The research, which has been carried on over a year and a half and has involved the preparation and microscopic examination of thousands of sections, is principally based on the application of improved hardening and staining methods to preparations from tube cultures of the grasses concerned, the leaves of which were infected at definite spots.

scholarly journals Variants of Leiomyoma: Histomorphological Study of Tumors of Myometrium

2011 ◽  
Vol 3 (2) ◽  
pp. 89-92 ◽  
Author(s):  
K Manjula ◽  
Kadam Satyanarayana Rao ◽  
HR Chandrasekhar
Keyword(s):  
Prospective Study ◽  
Malignant Tumor ◽  
Microscopic Examination ◽  
Vascular Invasion ◽  
Good Prognosis ◽  
Benign Tumors ◽  
Malignant Neoplasms ◽  
Coagulative Necrosis ◽  
Histological Features ◽  
Neoplastic Lesions

ABSTRACT Objectives To study the histomorphological features of variants of leiomyomas of myometrium in our institution from hysterectomy and myomectomy specimens. Methods In this prospective study, 1,845 hysterectomy and myomectomy specimens collected over a period of two years were studied. A detailed gross and microscopic examination of tumors of myometrium were made after fixing and staining the specimen with routine H&E. Results Neoplastic lesions of the myometrium were diagnosed in 441 (23.90%) of the total 1,845 specimens. Benign tumors were diagnosed in 440 cases, and all the benign tumors were leiomyomas except one case of adenomyoma. Leiomyoma was the most common tumor of the myometrium constituting 99.54%. Usual leiomyoma constituted for 95.45% and variants of leiomyomas were 4.55%. Malignant tumor of the myometrium was diagnosed in one hysterectomy specimen out of 1,845 specimens. Conclusion Variants of leiomyomas were relatively less, but it is important to differentiate them from malignant neoplasms of the myometrium, as they have good prognosis. Tumor size, nature of margin, presence or absence of vascular invasion, coagulative necrosis and cytological atypia are the most important histological features for differentiation.

Microscopic Examination of Human Femurs Buried at Washington Island, Wisconsin

1970 ◽  
Vol 35 (4) ◽  
pp. 463-465 ◽  
Author(s):  
G. Richard Peske ◽  
N. C. Tappen
Keyword(s):  
Microscopic Examination ◽  
Three Dimensional ◽  
Serial Sections ◽  
Histological Features ◽  
Age Determinations

AbstractShafts of human femurs from burials on Washington Island, Wisconsin dated ca. A.D. 200 were in good enough condition to allow decalcification, microscopic sectioning and routine staining. Many histological features of the bone are retained. Such procedures are less laborious than grinding undecalcified sections, allow three-dimensional observation of serial sections, and offer possible uses in age determinations of skeletons and paleopathology. The specimens give initial evidence that prompt burial prevents the formation of weathering cracks in bone.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Uranyl Acetate and Rotary Shadowing to Increase the Contrast of Small Aggregated Virus Particles

1969 ◽  
Vol 27 ◽  
pp. 424-425
Author(s):  
Lee F. Ellis ◽  
Richard M. Van Frank ◽  
Walter J. Kleinschmidt
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Uranyl Acetate ◽  
Interferon Inducer ◽  
Virus Particles ◽  
Staining Methods ◽  
Rotary Shadowing

The extract from Penicillum stoliniferum, known as statolon, has been purified by density gradient centrifugation. These centrifuge fractions contained virus particles that are an interferon inducer in mice or in tissue culture. Highly purified preparations of these particles are difficult to enumerate by electron microscopy because of aggregation. Therefore a study of staining methods was undertaken.

Artifact-free electron microscopic immunocytochemistry on rat brain tissue

1991 ◽  
Vol 49 ◽  
pp. 272-273
Author(s):  
Veronika Burmeister ◽  
N. Ludvig ◽  
P.C. Jobe
Keyword(s):  
Microscopic Examination ◽  
Free Electron ◽  
Electron Microscopic Examination ◽  
Ultrastructural Localization ◽  
Rabbit Serum ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Phosphate Buffered Saline ◽  
Rat Brain Tissue ◽  
Immune Rabbit

Electron microscopic immunocytochemistry provides an important tool to determine the ultrastructural distribution of various molecules in both normal and pathologic tissues. However, the specific immunostaining may be obscured by artifactual immunoreaction product, misleading the investigator. Previous observations show that shortening the incubation period with the primary antibody from the generally used 12-24 hours to 1 hour substantially reduces the artifactual immunostaining. We now extend this finding by the demonstration of artifact-free ultrastructural localization of the Ca2/calmodulindependent cyclic nucleotide phosphodiesterase (CaM-dependent PDE) immunoreactivity in brain.Anesthetized rats were perfused transcardially with phosphate-buffered saline followed by a fixative containing paraformaldehyde (4%) and glutaraldehyde (0.25%) in PBS. The brains were removed, and 40μm sections were cut with a vibratome. The sections were processed for immunocytochemistry as described by Ludvig et al. Both non-immune rabbit serum and specific CaM-dependent PDE antibodies were used. In both experiments incubations were at one hour and overnight. The immunostained sections were processed for electron microscopic examination.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

Energy-filtered imaging of polymer microstructure

1996 ◽  
Vol 54 ◽  
pp. 162-163
Author(s):  
K. Siangchaew ◽  
J. Bentley ◽  
M. Libera
Keyword(s):  
Energy Loss ◽  
Heavy Element ◽  
Spectroscopic Imaging ◽  
Data Set ◽  
Polymer Microstructure ◽  
Entire Energy ◽  
Staining Methods ◽  
Spectrum Imaging ◽  
Resolution Data

Energy-filtered electron-spectroscopic TEM imaging provides a new way to study the microstructure of polymers without heavy-element stains. Since spectroscopic imaging exploits the signal generated directly by the electron-specimen interaction, it can produce richer and higher resolution data than possible with most staining methods. There are basically two ways to collect filtered images (fig. 1). Spectrum imaging uses a focused probe that is digitally rastered across a specimen with an entire energy-loss spectrum collected at each x-y pixel to produce a 3-D data set. Alternatively, filtering schemes such as the Zeiss Omega filter and the Gatan Imaging Filter (GIF) acquire individual 2-D images with electrons of a defined range of energy loss (δE) that typically is 5-20 eV.

Ineffective Thrombopoiesis

1990 ◽  
Vol 48 (3) ◽  
pp. 266-267
Author(s):  
JM Radley ◽  
SL Ellis
Keyword(s):  
Microscopic Examination ◽  
Phosphate Buffer ◽  
Primary Myelofibrosis ◽  
Transient Increase ◽  
Major Difficulty ◽  
Adult Mice ◽  
Dense Cytoplasm

In effective thrombopoies is has been inferred to occur in several disease sates from considerations of megakaryocyte mass and platelet kinetics. Microscopic examination has demonstrated increased numbers of megakaryocytes, with a typical forms particularly pronounced, in primary myelofibrosis. It has not been documented if megakaryocyte ever fail to reach maturity in non-pathological situations. A major difficulty of establishing this is that the number of megakaryocytes normally present in the marrow is extremely low. A large transient increase in megakaryocytopoiesis can how ever be induced in mice by an injection of 5-fluorouracil. We have utilised this treatment and report here evidence for in effective thrombopoies is in healthy mice.Adult mice were perfused (2% glutaraldehyde in 0.08M phosphate buffer, pH 7.4) 8 days following an injection of 5-fluorouracil (150mg/kg). Femurs were subsequently decalcified in 10% neutral E.D.T.A. and embedded in Spurrs resin. Transverse sections of marrow revealed many megakaryocytes at various stages of maturity. Occasional megakaryocytes (less than 1%) were found to be under going degeneration prior to achieving full maturation and releasing cytoplasm as platelets. These cells were characterized by a peripheral rim of dense cytoplasm which enveloped a mass of organelles and vacuoles (Fig. 1). Numerous microtubules were foundaround and with in the organelle-rich zone (Fig 2).

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