scholarly journals Comparison of manual (TB Fluorescent stain kit) and automated (RAL stainer) fluorescent staining methods for direct microscopic examination of respiratory tract samples of patients with suspected mycobacteria infection

2019 ◽  
Author(s):  
Cengiz Çavusoglu ◽  
Ayşe Arslan
Keyword(s):  
Respiratory Tract ◽  
Microscopic Examination ◽  
Fluorescent Staining ◽  
Direct Microscopic Examination ◽  
Staining Methods ◽  
Fluorescent Stain

scholarly journals A Comparision of Laboratory Diagnostic Methods of Tuberculosis and Aetiology of Suspected Cases of Pulmonary Tuberculosis

2015 ◽  
Vol 11 (2) ◽  
pp. 1-6
Author(s):  
Chandra Prakash Bhatt ◽  
B Timalsina ◽  
B Kutu ◽  
R Pradhan ◽  
B Maharjan ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Microscopic Examination ◽  
Fluorescent Microscopy ◽  
Public Health Problem ◽  
Diagnostic Methods ◽  
Fluorescent Staining ◽  
Acid Fast Bacilli ◽  
Direct Microscopic Examination ◽  
Culture Positive ◽  
Culture Negative

Introduction: Tuberculosis remains a worldwide public health problem despite the highly effective drugs and vaccines are available making tuberculosis a preventable and curable disease. The objective of this study was to compare the different laboratory diagnostic methods of tuberculosis and determine its prevalence. Methodology: Morning sputum samples were collected from suspected cases of pulmonary tuberculosis and preceded for Ziehl Neelsen staining, fluorescent staining (auramin-O) and mycobacterium culture in Lowenstein Jensen medium. Results: Total 78 suspected cases of pulmonary tuberculosis were included in this study among them 53 were male and 25 were female. Out of 78 cases, 46 cases were found to be culture positive. In culture positive cases (83%) were found to be M. tuberculosis and (17%) were found to be slow grower, fine colonies, AFB positive but niacin test negative (mycobacteria other than M. tuberculosis). In the direct microscopic examination by Ziehl Neelsen stained smear 26 samples were found to be acid fast bacilli and one sample was culture negative but acid fast bacilli positive. In fluorescent stained smear 34 samples were found to be positive for acid fast bacilli and 5 samples were culture negative but acid fast bacilli positive. Culture was accepted as gold standard, the sensitivity of direct microscopic examination was found 56.5% for Ziehl Neelsen staining and 73.9% for fluorescent staining respectively. Conclusion: In culture positive cases M. tuberculosis and mycobacteria other than M. tuberculosis was found to be 83% and 17% respectively, it was found higher in male than female. Fluorescent microscopy is superior to Ziehl Neelsen microscopy but gives more false positive result than Z-N staining. Combining of Ziehl Neelsen and fluorescent staining is better than fluorescent staining alone.DOI: http://dx.doi.org/10.3126/saarctb.v11i2.12427  SAARC J TUBER LUNG DIS HIV/AIDS, 2014;XI(2), page: 1-6

scholarly journals PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES

1948 ◽  
Vol 32 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Winston H. Price
Keyword(s):  
Microscopic Examination ◽  
Synthetic Medium ◽  
Bacterial Virus ◽  
Direct Microscopic Examination ◽  
Infected Cells

1. The release of S. muscae phage in veal infusion medium is correlated with lysis of the host. 2. The release of the bacterial virus in Fildes' synthetic medium occurs in a step-wise manner before observable lysis of the cells occurs. This result has been confirmed by both turbidimetric readings and direct microscopic examination of the infected cells.

scholarly journals On the histology of Uredo dispersa , Erikss., and the 'mycoplasm’ hypothesis

1903 ◽  
Vol 71 (467-476) ◽  
pp. 353-354
Keyword(s):  
Microscopic Examination ◽  
Histological Features ◽  
Bromus Secalinus ◽  
Staining Methods

The paper deals with a detailed study of the histological features of the germination, infection, and growth of the mycelium of the Uredo in the tissue of grasses. Primarily, the figures refer especially to the Uredo of Puccinia dispersa in the tissues of Bromus secalinus , but comparisons are made with the behaviour of this and other Uredineæ— e. g ., Puccinia glumarum and P. gramins —in the tissues of other grasses and cereals. The research, which has been carried on over a year and a half and has involved the preparation and microscopic examination of thousands of sections, is principally based on the application of improved hardening and staining methods to preparations from tube cultures of the grasses concerned, the leaves of which were infected at definite spots.

scholarly journals CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Fajarudin Ahmad ◽  
Yuyu Suryasari Poerba
Keyword(s):  
Genetic Study ◽  
Cell Spreading ◽  
Enzymatic Digestion ◽  
Chromosome Count ◽  
Root Tip ◽  
Chromosome Counting ◽  
Fluorescent Staining ◽  
Dapi Staining ◽  
Slide Preparation ◽  
Staining Methods

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting. 

scholarly journals Comparison between ziehl-neelsen staining and fluorescent staining of sputum samples to detect acid fast bacilli in suspected case of pulmonary tuberculosis at tertiary care hospital, Amreli, Gujarat

2021 ◽  
Vol 8 (4) ◽  
pp. 302-307
Author(s):  
Hetvi Chawda ◽  
Chandani Surani ◽  
Sanjeev Kumar ◽  
Meghana Chauhan ◽  
Ashok Kumar Ramanuj ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Tertiary Care ◽  
Early Morning ◽  
Causative Organism ◽  
Sputum Smear ◽  
Suspected Case ◽  
Care Hospital ◽  
Fluorescent Staining ◽  
Acid Fast Bacilli ◽  
Fluorescent Stain

In India, Tuberculosis (TB) is one of the major community health problems.Pulmonary tuberculosis (PTB) is a respiratory disease. Causative organism for this is acid fast bacilli known as . It is the most ordinary disease affecting the lower socio-economic class in developing countries. Microbiological diagnosis is the heart for the effective treatment of pulmonary TB (PTB). The look forrapid and efficient method has resulted in several staining techniques. Objective of the study was to compare the results of ZN stain (RNTCP) with fluorescent stain by use of microscopy. The study was carried out in Microbiology Department, SMCGH, Amreli. 350 sputum samples (Spot and early morning sample) collected from 175 suspected case of the pulmonary tuberculosis. All 350 samples were processed by ZN stain and Fluorescent stain to detect acid fast bacilli. By use of microscope, the results of the stained smears were given according to RNTCP guideline.Out of 350 sputum smears, 52 (14.85%) and 61 (17.4%) were positive by ZN and FM staining respectively. Males are predominantly affected than females. Majority of the patients were in age above 50 years. Early morning samples were more reliable than spot samples for detection of acid fast bacilli for ZN stain, but not for fluorescent stain.Fluorescent staining with LED microscopy was more efficient than ZN staining for detection of acid fast bacilli from sputum smear.

scholarly journals Diagnosis of Cutaneous Leishmaniasis in Colombia: the Sampling Site within Lesions Influences the Sensitivity of Parasitologic Diagnosis

2000 ◽  
Vol 38 (10) ◽  
pp. 3768-3773 ◽  
Author(s):  
José Robinson Ramírez ◽  
Sonia Agudelo ◽  
Carlos Muskus ◽  
Juan Fernando Alzate ◽  
Christof Berberich ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Microscopic Examination ◽  
Sampling Site ◽  
Parasite Load ◽  
Diagnostic Methods ◽  
Active Margins ◽  
Direct Microscopic Examination ◽  
Chronic Ulcers ◽  
Pcr Method ◽  
Higher Sensitivity

Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrapings taken from the bottoms of ulcers (BDS) with that of dermal scrapings taken from indurated active margins of lesions (MDS) in a total of 115 patients. The sensitivities of the microscopic examination were 90.4 and 78.3% for BDS and MDS samples, respectively. When the PCR method was used with a group of 40 patients, we also observed a higher sensitivity when BDS samples were examined (80.8% in BDS samples versus 57.7% in MDS samples). The improvement of the diagnostic sensitivity in the BDS samples appears to be related to the higher parasite load and more easily detectable morphology of amastigotes in the centers of the ulcers. Other parasitologic diagnostic methods, such as culture and histopathologic examination of biopsies, are less sensitive (67.5 and 64.3%, respectively). Aspirate culture, however, was shown to be the most sensitive method for the diagnosis of patients with chronic ulcers. When microscopic examinations of both MDS and BDS samples are combined, the sensitivity of diagnosis may rise up to 94%. We therefore recommend this method as a primary routine procedure for diagnosis of cutaneous leishmaniasis.

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