The use of neutrons to study protein-RNA interactions

1980 ◽  
Vol 290 (1043) ◽  
pp. 627-633 ◽  
Keyword(s):  
Structural Information ◽  
Viral Proteins ◽  
Globular Proteins ◽  
Specific Interactions ◽  
Trna Synthetases ◽  
Structural Modifications ◽  
Aminoacyl Trna Synthetases ◽  
Simultaneous Study ◽  
And Function

Protein-RNA interactions play a key role in the structure, morphogenesis and function of various systems (viruses, ribosomes and, more generally, protein synthesis). The neutron is a powerful tool to study those interactions. Some examples, are given. For viruses, neutrons provide structural information on the two molecules where they interact. Viral proteins do not appear to be simple globular proteins. In the interactions between tRNA and aminoacyl tRNA synthetases, neutrons allow a simultaneous study of the reaction and of the structural modifications associated with that reaction, giving a hint on the role of both electrostatic and specific interactions.

Aminoacyl-tRNA Synthetases: Occurrence, Structure, and Function

tRNA ◽  
2014 ◽  
pp. 251-292 ◽  
Author(s):  
Thierry Meinnel ◽  
Yves Mechulam ◽  
Sylvain Blanquet
Keyword(s):  
Structure And Function ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases ◽  
And Function

scholarly journals The Role of Orthogonality in Genetic Code Expansion

Life ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 58 ◽  
Author(s):  
Pol Arranz-Gibert ◽  
Jaymin R. Patel ◽  
Farren J. Isaacs
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Genetic Code ◽  
Cross Reactivity ◽  
Elongation Factors ◽  
Translational Machinery ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases ◽  
Genetic Code Expansion

The genetic code defines how information in the genome is translated into protein. Aside from a handful of isolated exceptions, this code is universal. Researchers have developed techniques to artificially expand the genetic code, repurposing codons and translational machinery to incorporate nonstandard amino acids (nsAAs) into proteins. A key challenge for robust genetic code expansion is orthogonality; the engineered machinery used to introduce nsAAs into proteins must co-exist with native translation and gene expression without cross-reactivity or pleiotropy. The issue of orthogonality manifests at several levels, including those of codons, ribosomes, aminoacyl-tRNA synthetases, tRNAs, and elongation factors. In this concept paper, we describe advances in genome recoding, translational engineering and associated challenges rooted in establishing orthogonality needed to expand the genetic code.

scholarly journals Study of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases

1975 ◽  
Vol 2 (2) ◽  
pp. 211-222 ◽  
Author(s):  
Jacques Bonnet ◽  
Nicole Befort ◽  
Claudine Bollack ◽  
Franco Fasiolo ◽  
Jean-Pierre Ebel
Keyword(s):  
Trna Synthetases ◽  
Acceptor Stem ◽  
Aminoacyl Trna Synthetases

scholarly journals Inflammatory Mediators in Vascular Disease: Identifying Promising Targets for Intracranial Aneurysm Research

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
David M. Sawyer ◽  
Peter S. Amenta ◽  
Ricky Medel ◽  
Aaron S. Dumont
Keyword(s):  
Intracranial Aneurysm ◽  
Intracranial Aneurysms ◽  
Future Research ◽  
Specific Interactions ◽  
Effector Molecules ◽  
Inflammatory Processes ◽  
And Function ◽  
Molecular Patterns ◽  
Damage Associated Molecular Patterns

Inflammatory processes are implicated in many diseases of the vasculature and have been shown to play a key role in the formation of intracranial aneurysms (IAs). Although the specific mechanisms underlying these processes have been thoroughly investigated in related pathologies, such as atherosclerosis, there remains a paucity of information regarding the immunopathology of IA. Cells such as macrophages and lymphocytes and their effector molecules have been suggested to be players in IA, but their specific interactions and the role of other components of the inflammatory response have yet to be determined. Drawing parallels between the pathogenesis of IA and other vascular disorders could provide a roadmap for developing a mechanistic understanding of the immunopathology of IA and uncovering useful targets for therapeutic intervention. Future research should address the presence and function of leukocyte subsets, mechanisms of leukocyte recruitment and activation, and the role of damage-associated molecular patterns in IA.

Sign in / Sign up

Export Citation Format

Share Document