scholarly journals 'Pathogenesis-related' P1(p14) Protein. Vacuolar and Apoplastic Localization in Leaf Tissue from Tomato Plants Infected with Citrus Exocortis Viroid; in vitro Synthesis and Processing

1989 ◽  
Vol 70 (8) ◽  
pp. 1933-1942 ◽  
Author(s):  
P. Vera ◽  
J. Hernandez-Yago ◽  
V. Conejero
Keyword(s):  
Leaf Tissue ◽  
In Vitro Synthesis ◽  
Tomato Plants ◽  
Citrus Exocortis Viroid ◽  
Pathogenesis Related ◽  
Synthesis And Processing

scholarly journals Three mutations in Zea mays affecting zein accumulation: a comparison of zein polypeptides, in vitro synthesis and processing, mRNA levels, and genomic organization.

1982 ◽  
Vol 94 (1) ◽  
pp. 201-206 ◽  
Author(s):  
F A Burr ◽  
B Burr
Keyword(s):  
Genomic Organization ◽  
Protein Body ◽  
Large Deletion ◽  
Size Class ◽  
Mrna Levels ◽  
In Vitro Synthesis ◽  
Concomitant Reduction ◽  
Synthesis And Processing ◽  
Zein Genes

We studied three mutations, opaque-2 (o2), opaque-7 (o7), and floury-2(fI2), each of which causes a depression in zein synthesis. We examined the processing efficiencies of the rough endoplasmic reticulum membranes in vitro, the levels of RNA transcription using cloned zein probes, and the genomic organization of the zein sequences as possible sites for the genetic defects. The results obtained indicate that the steps in prezein translation and processing occurring on the protein body membranes are not accountable for the lowered zein content in any of the mutations. The o2 mutation that typically shows a paucity of 22.5-kdalton zein polypeptides was found to have a concomitant reduction in a particular subgroup of mRNAs coding for this size class. Southern analyses suggest that the o2 mutation is not the result of a large deletion of tandem-linked zein genes.

scholarly journals Sequence-specific priming of the in vitro synthesis of DNA complementary to citrus exocortis viroid

FEBS Letters ◽  
1981 ◽  
Vol 130 (2) ◽  
pp. 208-212 ◽  
Author(s):  
Wolfgang Rohde ◽  
Martina Schnölzer ◽  
Heinz L. Sänger
Keyword(s):  
In Vitro Synthesis ◽  
Citrus Exocortis Viroid ◽  
Specific Priming

In vitro transcription of viroid RNA into full-length copies by RNA-dependent RNA polymerase from healthy tomato leaf tissue

1982 ◽  
Vol 2 (3) ◽  
pp. 185-194 ◽  
Author(s):  
Frank Boege ◽  
Wolfgang Rohde ◽  
Heinz L. Sänger
Keyword(s):  
Rna Polymerase ◽  
Tomato Plant ◽  
Leaf Tissue ◽  
Potato Spindle Tuber Viroid ◽  
In Vitro Transcription ◽  
Tomato Leaf ◽  
Full Length ◽  
Rna Dependent Rna Polymerase ◽  
In Vitro Synthesis

RNA-dependent RNA polymerase from healthy tomato plant tissue accepts potato spindle tuber viroid (PSTV) RNA as a template for the in vitro synthesis of full-length RNA copies of the PSTV genome. Viroid transcription requires the presence of Mn2+ and/or Mg2+ ions and is not inhibited by concentrations of 10−5 M α-amanitin. This is the first report of a well-defined product synthesized in vitro by an RNA-dependent RNA polymerase from healthy plants.

scholarly journals In Vitro Synthesis and Processing of Tomato Fruit Polygalacturonase

1988 ◽  
Vol 86 (4) ◽  
pp. 1057-1063 ◽  
Author(s):  
Dean DellaPenna ◽  
Alan B. Bennett
Keyword(s):  
Tomato Fruit ◽  
In Vitro Synthesis ◽  
Synthesis And Processing

scholarly journals In Vitro Synthesis and Processing of Wheat α-Amylase

1982 ◽  
Vol 69 (1) ◽  
pp. 150-154 ◽  
Author(s):  
Rebecca S. Boston ◽  
Timothy J. Miller ◽  
Janet E. Mertz ◽  
Richard R. Burgess
Keyword(s):  
In Vitro Synthesis ◽  
Synthesis And Processing
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