An aberrant genotype revealed in recombinant hepatitis B virus strains from Vietnam

Six genotypes of hepatitis B virus (HBV) have been described. However, relatively few complete genomes originating from East Asia, where most of the world’s HBV carriers live, have been studied. We analysed five complete HBV genomes of Vietnamese origin, which in our previous studies had produced atypical genotyping patterns. All five strains had HBsAg sequences with markers for serotype adw. In phylogenetic tree analysis, two of the genomes clustered with genotype C, and three clustered on a separate branch between genotypes A, B and C, suggesting a new genotype. However, these three strains showed signs of recombination in similarity plot and bootscanning analysis. Phylogenetic tree analysis of two segments separately supported recombination between genotype C and a putative new genotype (or possibly a subgroup of genotype A). The segment between nt 1801 and 2865 was clearly of genotype C origin, while the major part of the genome (nt 2866–1800) was placed on a branch close to genotype A. The findings encourage further study of genotypes and recombination in HBV from this geographical region.

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Molecular Analysis of Hepatitis B Virus in Mothers-Children Pairs

Austin Journal of Infectious Diseases ◽

10.26420/austinjinfectdis.2021.1045 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Chatzidaki V ◽

◽

Perdikogianni C ◽

Galanakis E ◽

Paraskevis D ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Phylogenetic Tree ◽

Surface Glycoprotein ◽

Phylogenetic Tree Analysis ◽

Source Of Infection ◽

B Virus ◽

Maximum Likelihood Methods ◽

Infection Source ◽

Relationship Of

ackground: Vertical transmission of Hepatitis B Virus (HBV) is the primary infection source for infants, but little is known on the proportion of children that have acquired HBV from their mothers. Objective: We investigated the relationship of HBV sequencing in HBVpositive children and their mothers and explored the HBV phylogenetic tree. Methods: Serum-extracted HBV-DNA from 38 individuals (13 children paired to nine mothers, 16 unpaired infected children) was amplified by polymerase chain reaction and the target region HBV surface glycoprotein (amino acids 40-171) was directly sequenced. Following editing and alignment of these sequences, phylogenetic tree analysis was performed using the neighbourjoining and maximum-likelihood methods. Results: Analysis was successfully performed in 29 subjects (23 children and six mothers), including six mother-child pairs. All individuals were infected by genotype D. Subgenotype adw3 prevailed (21, 72.4%), followed by ayw2 (4, 13.8%) and ayw3 (4, 13.8%). Among six mother-child pairs, three had identical and three had different subgenotypes. Phylogenetic analysis revealed that HBV sequences from three children did not cluster with their siblings suggesting a different source of infection. Conclusion: Our findings suggest that HBV subgenotypes in infected children may not be identical to their mothers’ and point to non-vertical HBV transmission in childhood.

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Effectiveness of genotype C-derived hepatitis B vaccine in preventing perinatal transmission in children born to hepatitis B virus carrier mothers infected with genotype A

Kanzo ◽

10.2957/kanzo.56.675 ◽

2015 ◽

Vol 56 (12) ◽

pp. 675-677

Author(s):

Haruki Komatsu ◽

Kentaro Iwasawa ◽

Ayano Inui ◽

Tomoyuki Tsunoda ◽

Shuichiro Umetsu ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Perinatal Transmission ◽

Hepatitis B Vaccine ◽

B Virus ◽

Hepatitis B Virus Carrier ◽

Genotype C ◽

Genotype A ◽

Virus Carrier

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Minimum infectious dose of hepatitis B virus in chimpanzees and difference in the dynamics of viremia between genotype A and genotype C

Transfusion ◽

10.1111/j.1537-2995.2007.01522.x ◽

2007 ◽

Vol 0 (0) ◽

pp. 071121030748004-??? ◽

Cited By ~ 6

Author(s):

Yutaka Komiya ◽

Keiko Katayama ◽

Hisao Yugi ◽

Masaaki Mizui ◽

Harumichi Matsukura ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Infectious Dose ◽

B Virus ◽

Genotype C ◽

Genotype A

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Detection of Hepatitis B Genotypes in HBsAg & HBeAg Carriers

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v7i5.556 ◽

2018 ◽

Vol 7 (5) ◽

Author(s):

Salman Khan ◽

Molly Madan

Keyword(s):

Chronic Hepatitis ◽

Viral Load ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Central Research ◽

Elisa Kit ◽

B Virus ◽

Negative Chronic Hepatitis ◽

Genotype A

Objective:- Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between march 2016 to November 2017 The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying Results:. Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected Conclusions: Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers & HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers & genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region Keywords: Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR

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