scholarly journals Comparison of PCR fingerprinting, by random amplification of polymorphic DNA, with other molecular typing methods for Candida albicans

1993 ◽  
Vol 139 (9) ◽  
pp. 2179-2184 ◽  
Author(s):  
A. Bostock ◽  
M. N. Khattak ◽  
R. Matthews ◽  
J. Burnie
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Pcr Fingerprinting ◽  
Random Amplification ◽  
Typing Methods

scholarly journals MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

2013 ◽  
Vol 55 (6) ◽  
pp. 385-391 ◽  
Author(s):  
Patricia de Souza Bonfim-Mendonca ◽  
Adriana Fiorini ◽  
Cristiane Suemi Shinobu-Mesquita ◽  
Lilian Cristiane Baeza ◽  
Maria Aparecida Fernandez ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Blood Culture ◽  
Genetic Variability ◽  
Molecular Typing ◽  
Fungal Infections ◽  
Common Origin ◽  
High Similarity ◽  
Candida Spp ◽  
Typing Methods ◽  
A Site

SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.

scholarly journals Comparison of molecular typing methods for Candida albicans.

1992 ◽  
Vol 30 (10) ◽  
pp. 2674-2679 ◽  
Author(s):  
P T Magee ◽  
L Bowdin ◽  
J Staudinger
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Typing Methods

scholarly journals Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan

2005 ◽  
Vol 54 (3) ◽  
pp. 249-258 ◽  
Author(s):  
Kuo-Wei Chen ◽  
Hsiu-Jung Lo ◽  
Yu-Hui Lin ◽  
Shu-Ying Li
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Genetic Relatedness ◽  
Geographic Origin ◽  
Discriminatory Power ◽  
Patient Specific ◽  
High Discriminatory Power ◽  
Typing Methods ◽  
Pcr Method ◽  
Genetic Profiles

This report describes the investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of SfiI and BssHII restriction fragments) and one repetitive-sequence-PCR (rep-PCR) method. All four methods were able to identify clonal related isolates from the same patients. PFGE-BssHII exhibited the highest discriminatory power by discriminating 40 genotypes, followed by PFGE-SfiI (35 genotypes) and then by rep-PCR (31 genotypes), while PFGE karyotyping exhibited the lowest discriminatory power (19 genotypes). High discriminatory power can also be achieved by combining typing methods with different typing mechanisms, such as rep-PCR and PFGE-based typing methods. The results also showed that the genotype of each isolate was patient-specific and not associated with the source of the isolation, geographic origin or antifungal resistance.

scholarly journals DNA fingerprinting of medically important microorganisms by use of PCR.

1994 ◽  
Vol 7 (2) ◽  
pp. 174-184 ◽  
Author(s):  
A van Belkum
Keyword(s):  
Dna Fingerprinting ◽  
Molecular Typing ◽  
Microbial Genetics ◽  
Dna Molecule ◽  
Pcr Fingerprinting ◽  
Current State ◽  
Typing Methods

Selected segments of any DNA molecule can be amplified exponentially by PCR. This technique provides a powerful tool to detect and identify minimal numbers of microorganisms. PCR is applicable both in diagnosis and in epidemiology. By amplification of hypervariable DNA domains, differences can be detected even among closely related strains. PCR fingerprinting is a valuable tool for medical microbiologists, epidemiologists, and microbial taxonomists. The current state of PCR-mediated genotyping is reviewed, and a comparison with conventional molecular typing methods is included. Because of its speed and versatility, PCR fingerprinting will play an important role in microbial genetics, epidemiology, and systematics.

An Outbreak of ESBL-producing Klebsiella pneumoniae in an Iranian Referral Hospital: Epidemiology and Molecular Typing

2019 ◽  
Vol 19 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Shima Mahmoudi ◽  
Babak Pourakbari ◽  
Aliakbar Rahbarimanesh ◽  
Mohammad Reza Abdosalehi ◽  
Keyghobad Ghadiri ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infections ◽  
Molecular Typing ◽  
Genetic Relatedness ◽  
Referral Hospital ◽  
Limited Information ◽  
Hospital Epidemiology ◽  
Rapd Pcr ◽  
Published Report ◽  
Random Amplification

Introduction: Klebsiella pneumoniae is a common cause of nosocomial infections; however, there is limited information in Iran regarding nosocomial outbreaks due to extended-spectrum β–lactamase (ESBL) producing K pneumoniae strains, particularly using molecular methods. The present study focused on the molecular mechanism of ESBL resistance and genetic relatedness in K. pneumoniae isolates causing nosocomial infections in an Iranian referral hospital. Material and Methods: This study evaluated the antimicrobial resistance and molecular epidemiology of K. pneumoniae causing nosocomial infections in children between October 2013 and March 2014. The ESBL detection was carried out for all the isolates by the CLSI method and PCR was carried out for the detection of the blaSHV, blaTEM, and blaCTX-M genes among ESBL-producing K. pneumonia. Molecular typing of the K. pneumoniae was performed using random amplification of polymorphic DNA-polymerase chain reaction (RAPD-PCR). Results: A total of 30 isolates of K. pneumoniae were used for epidemiological analysis. High rates of resistance to cefotaxime (n=29, 97%), cefazolin (n=29, 97%), cefepime (n=25, 83%) and gentamicin (n=23, 77%) were observed. A total of 29 strains (97%) produced ESBLs. The frequency of blaSHV, blaCTX-M and blaTEM genes among these isolates was 83% (n=25), 70% (n=21) and 57% (n=17), respectively. Surprisingly 11 isolated (37%) carried blaSHV, blaCTX-M and blaTEM genes simultaneously. Moreover, the concurrent presence of “blaSHV and blaCTX-M” and “blaSHV and blaTEM” was seen in 8 (27%) and 4 (13%) isolates, respectively. RAPDPCR analyses revealed that K. pneumoniae isolates belonged to 2 RAPD-PCR types among which one cluster counted for 28 isolates. Conclusion: To our knowledge, this is the first published report of a nosocomial outbreak of ESBL-producing K. pneumoniae in children in Iran. Although the epidemiology of nosocomial infections with ESBL-producing organisms has not yet been explored in depth in Iran, our findings suggest that ESBL-producing organisms are already an established public health threat in our country.

Molecular typing of multi-drug resistant Candida albicans isolated from the Segamat community, Malaysia

2021 ◽  
Author(s):  
Marie Andrea Laetitia Huët ◽  
Nazmul Hasan Muzahid ◽  
Chuen Zhang Lee ◽  
Calvin Bok Sun Goh ◽  
Jacky Dwiyanto ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Drug Resistant

scholarly journals Strain Typing of Trichosporon asahii Clinical Isolates by Random Amplification of Polymorphic DNA (RAPD) Analysis

2021 ◽  
Author(s):  
Thayanidhi Premamalini ◽  
Vijayaraman Rajyoganandh ◽  
Ramaraj Vijayakumar ◽  
Hemanth Veena ◽  
Anupma Jyoti Kindo ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Strain Typing ◽  
Trichosporon Asahii ◽  
Pcr Fingerprinting ◽  
Specific Pcr ◽  
Rapd Primer ◽  
Random Amplification

Abstract Objective The aim of this study was to identify and isolate Trichosporon asahii (T. asahii) from clinical samples and to assess the genetic relatedness of the most frequently isolated strains of T. asahii using random amplification of polymorphic DNA (RAPD) primers GAC-1 and M13. Methods All the clinical samples that grew Trichosporon species, identified and confirmed by polymerase chain reaction (PCR) using Trichosporon genus-specific primers, were considered for the study. Confirmation of the species T. asahii was carried out by T. asahii-specific PCR. Fingerprinting of the most frequently isolated T. asahii isolates was carried out by RAPD using random primers GAC-1 and M13. Results Among the 72 clinical isolates of Trichosporon sp. confirmed by Trichosporon-specific PCR, 65 were found to be T. asahii as identified by T. asahii-specific PCR. Fingerprinting of the 65 isolates confirmed as T. asahii using GAC-1 RAPD primer yielded 11 different patterns, whereas that of M13 primer produced only 5 patterns. The pattern I was found to be the most predominant type (29.2%) followed by pattern III (16.9%) by GAC-1 primer. Conclusions This study being the first of its kind in India on strain typing of T. asahii isolates by adopting RAPD analysis throws light on genetic diversity among the T. asahii isolates from clinical samples. Fingerprinting by RAPD primer GAC-1 identified more heterogeneity among the T. asahii isolates than M13.

Outbreak ofStenotrophomonas maltophiliaBacteremia Among Patients Undergoing Bone Marrow Transplantation: Association With Faulty Replacement of Handwashing Soap

1999 ◽  
Vol 20 (11) ◽  
pp. 756-758 ◽  
Author(s):  
Jeffrey D. Klausner ◽  
Carol Zukerman ◽  
Ajit P. Limaye ◽  
Lawrence Corey
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Healthcare Worker ◽  
Molecular Typing ◽  
Marrow Transplantation ◽  
Stenotrophomonas Maltophilia ◽  
Marrow Transplant ◽  
Hand Washing ◽  
Transplant Patients ◽  
Typing Methods

AbstractUsing molecular typing methods, we confirmed an outbreak ofStenotrophomonas maltophiliaamong bone marrow transplant patients. The likely source was a healthcare worker who may have washed with moisturizer instead of soap between patients. Hospital epidemiologists need to go beyond antibiograms when evaluating outbreaks and be vigilant about all aspects of hand washing.

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