scholarly journals Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan

2005 ◽  
Vol 54 (3) ◽  
pp. 249-258 ◽  
Author(s):  
Kuo-Wei Chen ◽  
Hsiu-Jung Lo ◽  
Yu-Hui Lin ◽  
Shu-Ying Li
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Genetic Relatedness ◽  
Geographic Origin ◽  
Discriminatory Power ◽  
Patient Specific ◽  
High Discriminatory Power ◽  
Typing Methods ◽  
Pcr Method ◽  
Genetic Profiles

This report describes the investigation of the genetic profiles of 53 Candida albicans isolates collected from 18 hospitals in Taiwan using three PFGE-based typing methods (PFGE karyotyping, and PFGE of SfiI and BssHII restriction fragments) and one repetitive-sequence-PCR (rep-PCR) method. All four methods were able to identify clonal related isolates from the same patients. PFGE-BssHII exhibited the highest discriminatory power by discriminating 40 genotypes, followed by PFGE-SfiI (35 genotypes) and then by rep-PCR (31 genotypes), while PFGE karyotyping exhibited the lowest discriminatory power (19 genotypes). High discriminatory power can also be achieved by combining typing methods with different typing mechanisms, such as rep-PCR and PFGE-based typing methods. The results also showed that the genotype of each isolate was patient-specific and not associated with the source of the isolation, geographic origin or antifungal resistance.

scholarly journals High-Resolution Genotyping of Streptococcus pyogenesSerotype M1 Isolates by Fluorescent Amplified-Fragment Length Polymorphism Analysis

1999 ◽  
Vol 37 (6) ◽  
pp. 1948-1952 ◽  
Author(s):  
Meeta Desai ◽  
Androulla Efstratiou ◽  
Robert George ◽  
John Stanley
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Origin ◽  
Amplified Fragment Length Polymorphism ◽  
Discriminatory Power ◽  
Polymorphism Analysis ◽  
Worldwide Distribution ◽  
High Discriminatory Power ◽  
Typing Methods

We have used fluorescent amplified-fragment length polymorphism (FAFLP) analysis to subtype clinical isolates of Streptococcus pyogenes serotype M1. Established typing methods define most M1 isolates as members of a clone that has a worldwide distribution and that is strongly associated with invasive diseases. FAFLP analysis simultaneously sampled 90 to 120 loci throughout the M1 genome. Its discriminatory power, precision, and reproducibility were compared with those of other molecular typing methods. Irrespective of disease symptomatology or geographic origin, the majority of the clinical M1 isolates shared a single ribotype, pulsed-field gel electrophoresis macrorestriction profile, and emm1 gene sequence. Nonetheless, among these isolates, FAFLP analysis could differentiate 17 distinct profiles, including seven multi-isolate groups. The FAFLP profiles of M1 isolates reproducibly exhibited between 1 and more than 20 amplified fragment differences. The high discriminatory power of genotyping by FAFLP analysis revealed genetic microheterogeneity and differentiated otherwise “identical” M1 isolates as members of a clone complex.

scholarly journals MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

2013 ◽  
Vol 55 (6) ◽  
pp. 385-391 ◽  
Author(s):  
Patricia de Souza Bonfim-Mendonca ◽  
Adriana Fiorini ◽  
Cristiane Suemi Shinobu-Mesquita ◽  
Lilian Cristiane Baeza ◽  
Maria Aparecida Fernandez ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Blood Culture ◽  
Genetic Variability ◽  
Molecular Typing ◽  
Fungal Infections ◽  
Common Origin ◽  
High Similarity ◽  
Candida Spp ◽  
Typing Methods ◽  
A Site

SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.

scholarly journals Evaluation of BOX-PCR and ERIC-PCR as Molecular Typing Tools for PathogenicLeptospira

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Lesley Maurice Bilung ◽  
Chai Fung Pui ◽  
Lela Su’ut ◽  
Kasing Apun
Keyword(s):  
Genetic Distance ◽  
Molecular Typing ◽  
Genetic Relatedness ◽  
Mortality Rates ◽  
Health Concern ◽  
Public Health Concern ◽  
Zoonotic Pathogen ◽  
Future Studies ◽  
Typing Methods ◽  
Discriminatory Index

In the last decades, leptospirosis had gained public health concern due to morbidity and mortality rates caused by pathogenicLeptospira. The need for rapid and robust molecular typing methods to differentiate this zoonotic pathogen is of utmost importance. Various studies had been conducted to determine the genetic relatedness ofLeptospiraisolates using molecular typing methods. In this study, 29 pathogenicLeptospiraisolates from rat, soil, and water samples in Sarawak, Malaysia, were characterized using BOX-PCR and ERIC-PCR. The effectiveness of these two methods with regard to the ease of interpretation, reproducibility, typeability, and discriminatory power was also being evaluated. Using BOX-PCR, six clusters and 3 single isolates were defined at a genetic distance percentage of 11.2%. ERIC-PCR clustered the isolates into 6 clusters and 2 single isolates at a genetic distance percentage of 6.8%. Both BOX-PCR and ERIC-PCR produced comparable results though the discriminatory index for ERIC-PCR (0.826) was higher than that for BOX-PCR (0.809). From the constructed dendrogram, it could be summarized that the isolates in this study were highly heterogeneous and genetically diverse. The findings from this study indicated that there is no genetic relatedness among the pathogenicLeptospiraisolates in relation to the locality, source, and identity, with some exceptions. Out of the 29 pathogenicLeptospiraisolates studied, BOX-PCR and ERIC-PCR successfully discriminated 4 isolates (2 isolates each) into the same cluster in relation to sample sources, as well as 2 isolates into the same cluster in association with the sample locality. Future studies shall incorporate the use of other molecular typing methods to make a more thorough comparison on the genetic relatedness of pathogenicLeptospira.

scholarly journals Comparison of molecular typing methods for Candida albicans.

1992 ◽  
Vol 30 (10) ◽  
pp. 2674-2679 ◽  
Author(s):  
P T Magee ◽  
L Bowdin ◽  
J Staudinger
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Typing Methods

Molecular typing and genetic relatedness of 72 clinical Candida albicans isolates from poultry

2018 ◽  
Vol 214 ◽  
pp. 36-43 ◽  
Author(s):  
Jianchai Liu ◽  
Huanzhang Liu ◽  
Jinkun Yan ◽  
Na Liu ◽  
Heping Zhang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Molecular Typing ◽  
Genetic Relatedness

scholarly journals Molecular typing methods and their discriminatory power

1998 ◽  
Vol 4 (2) ◽  
pp. 61-63 ◽  
Author(s):  
Dominique S. Blanc ◽  
Philippe M. Hauser ◽  
Patrick Francioli ◽  
Jacques Bille
Keyword(s):  
Molecular Typing ◽  
Discriminatory Power ◽  
Typing Methods

scholarly journals Conventional and Molecular Typing of Salmonella enterica serotype Typhi Locally Isolated In Baghdad

2012 ◽  
Vol 9 (4) ◽  
pp. 632-639
Author(s):  
Baghdad Science Journal
Keyword(s):  
Molecular Typing ◽  
Salmonella Enterica ◽  
Random Amplified Polymorphic Dna ◽  
Discriminatory Power ◽  
Universal Primers ◽  
Pcr Analysis ◽  
Biochemical Tests ◽  
Promising Alternative ◽  
Rapd Pcr ◽  
Typing Methods

Phenotypic And genotypic characteristics of Salmonella enterica serotype Typhi have been determined for 29 isolates, from Baghdad in 2007. Conventional typing methods were performed by biochemical tests, and antimicrobial susceptibility test. Molecular typing performed by analysis plasmid DNA beside using the Random Amplified Polymorphic DNA (RAPD-PCR). For the latter, two universal primers that have selected for the high discriminatory power were used for RAPD analysis. All isolates were belong one biotype according to the differention by their ability to decarboxylat lysine, 29(100%) were lysine (+). All the isolates were susceptible to the Antibiotics used. However, all the strains free of plasmids. RAPD was capable of grouping the strains in 6 genotypic patterns using primer 784, in 4 genotypic patterns using primer 787. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented non significant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and can be considered as a promising alternative typing method for S. Typhi.

scholarly journals CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods

2021 ◽  
Vol 12 ◽  
Author(s):  
Clémence Beauruelle ◽  
Ludovic Treluyer ◽  
Adeline Pastuszka ◽  
Thierry Cochard ◽  
Clément Lier ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Diversity Index ◽  
Reference Method ◽  
Direct Repeat ◽  
Discriminatory Power ◽  
High Discriminatory Power ◽  
Typing Methods ◽  
Group B ◽  
Reduced Time ◽  
Capsular Typing

We explored the relevance of a Clustered regularly interspaced short palindromic repeats (CRISPR)-based genotyping tool for Streptococcus agalactiae typing and we compared this method to current molecular methods [multi locus sequence typing (MLST) and capsular typing]. To this effect, we developed two CRISPR marker schemes (using 94 or 25 markers, respectively). Among the 255 S. agalactiae isolates tested, 229 CRISPR profiles were obtained. The 94 and 25 markers made it possible to efficiently separate isolates with a high diversity index (0.9947 and 0.9267, respectively), highlighting a high discriminatory power, superior to that of both capsular typing and MLST (diversity index of 0.9017 for MLST). This method has the advantage of being correlated with MLST [through analysis of the terminal direct repeat (TDR) and ancestral spacers] and to possess a high discriminatory power (through analysis of the leader-end spacers recently acquired, which are the witnesses of genetic mobile elements encountered by the bacteria). Furthermore, this “one-shot” approach presents the benefit of much-reduced time and cost in comparison with MLST. On the basis of these data, we propose that this method could become a reference method for group B Streptococcus (GBS) typing.

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