Experimental infections with protease-deficient mutants of Pseudomonas aeruginosa in mice (Plate VIII)

S-sulfonated gamma globulin (GGS), newly developed as a safe drug for intravenous use, was studied for its protective effects against some experimental infections in mice. Gamma globulin showed a good protective activity against infections due to Streptococcus pneumoniae and Escherichia coli and was moderately active against infections due to Staphylococcus aureus and Pseudomonas aeruginosa. In most cases, the potency of GGS was almost the same as that of original native gamma globulin. The duration of GGS activity in vivo was found to be comparable to that of native gamma globulin and much higher than that of pepsin-digested gamma globulin. In the control of infection due to E. coli, specific antibody was found to play a central role in the antibacterial action of GGS. When GGS was administered in combination with the antibiotics gentamicin and cefazolin for the control of infections due to S. pneumoniae or E. coli, a clear synergistic effect was observed.

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Alkaline protease-deficient mutants of Pseudomonas aeruginosa are virulent in the eye

Current Eye Research ◽

10.1076/0271-3683(200009)2131-rft730 ◽

2000 ◽

Vol 21 (3) ◽

pp. 730-739 ◽

Cited By ~ 8

Author(s):

C.M. Pillar ◽

L.D. Hazlett ◽

J.A. Hobden

Keyword(s):

Pseudomonas Aeruginosa ◽

Alkaline Protease ◽

Protease Deficient

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Role of Exotoxin and Protease as Possible Virulence Factors in Experimental Infections with Pseudomonas aeruginosa

Infection and Immunity ◽

10.1128/iai.19.3.839-845.1978 ◽

1978 ◽

Vol 19 (3) ◽

pp. 839-845 ◽

Cited By ~ 53

Author(s):

Kathleen Snell ◽

Ian A. Holder ◽

Stephen A. Leppla ◽

Catharine B. Saelinger

Keyword(s):

Pseudomonas Aeruginosa ◽

Virulence Factors ◽

Experimental Infections

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Role of exotoxin A and proteases of Pseudomonas aeruginosa in respiratory tract infections

Canadian Journal of Microbiology ◽

10.1139/m82-033 ◽

1982 ◽

Vol 28 (2) ◽

pp. 248-255 ◽

Cited By ~ 15

Author(s):

Neil R. Baker

Keyword(s):

Pseudomonas Aeruginosa ◽

Protein Synthesis ◽

Respiratory Tract ◽

Detectable Effect ◽

Ciliary Activity ◽

Exotoxin A ◽

Protease Deficient ◽

Tract Infections ◽

Cellular Swelling

Hamster tracheal organ culture has been used to study the role of exotoxin A and proteases of Pseudomonas aeruginosa in infections of the respiratory tract. Tracheal explants infected with a toxin producing, protease producing strain or a toxin deficient, protease producing strain displayed evidence of exfoliation and disorganization of the tracheal epithelium 12 h after initiation of infection. A toxin producing protease deficient strain caused some desquamation of cells and cellular swelling, but exfoliation was not evident. Each of the toxin producing and (or) protease producing strains inhibited protein synthesis of the explants. Purified exotoxin inhibited protein synthesis and caused some pathological changes similar to those observed with the toxin producing strain. Purified elastase from P. aeruginosa only inhibited protein synthesis at 10 μg/mL but caused exfoliation at 0.01 μg/mL. Alkaline protease had no detectable effect on the explants. The effects of active infection could be prevented by treatment with gentamycin. All the strains tested caused an inhibition of ciliary activity, but no correlation could be made with any of the extracellular products. Exotoxin A and elastase may be responsible for much of the destruction of respiratory tract tissue in Pseudomonas infections although other bacterial factors and host factors are of importance.

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