scholarly journals Aestuariicella hydrocarbonica gen. nov., sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from a sea tidal flat

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1935-1940 ◽  
Author(s):  
Naysim Lo ◽  
Kyung Hyun Kim ◽  
Kyunghwa Baek ◽  
Baolei Jia ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Molecular Features ◽  
Gram Staining ◽  
Degrading Bacterium ◽  
Optimum Ph ◽  
Novel Genus And Species

A Gram-staining-negative, strictly aerobic bacterial strain, designated SM-6T, was isolated from a sea tidal flat of the Dangjin bay, South Korea. Strain SM-6T was able to degrade a broad range of aliphatic hydrocarbons. Cells were catalase- and oxidase-positive and non-motile rods. Growth of strain SM-6T was observed at 10–37 °C (optimum, 20–25 °C), at pH 5.5–9.0 (optimum, pH 6.5–7.5) and in the presence of 0–10 % (w/v) NaCl (optimum, 2–3 %). The only isoprenoid quinone detected was ubiquinone-8 (Q-8). C17: 1ω8c, C11: 0 3-OH, summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C9: 0 3-OH and C10: 0 3-OH were observed as the major cellular fatty acids and phosphatidylethanolamine, phosphatidylglycerol and four unidentified lipids were detected as polar lipids. The G+C content of the genomic DNA was 47.5 mol%. Strain SM-6T was most closely related to Pseudomaricurvus alkylphenolicus KU41GT (95.5 %), Maricurvus nonylphenolicus KU41ET (94.4 %) and Pseudoteredinibacter isoporae SW-11T (94.3 %), based on 16S rRNA gene sequences, and phylogenetic analyses showed that strain SM-6T formed a phyletic lineage distinct from the closely related genera. On the basis of phenotypic, chemotaxonomic and molecular features, strain SM-6T represents a novel genus and species of the order Alteromonadales in the class Gammaproteobacteria, for which name Aestuariicella hydrocarbonica gen. nov., sp. nov. is proposed. The type strain is SM-6T ( = KACC 18121T = JCM 30134T).

scholarly journals Luteimonas lutimaris sp. nov., isolated from a tidal flat

2011 ◽  
Vol 61 (11) ◽  
pp. 2729-2733 ◽  
Author(s):  
Youn-Je Park ◽  
Moon Su Park ◽  
Seung Hyeon Lee ◽  
Woojun Park ◽  
Kangseok Lee ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Gram Staining ◽  
Optimum Ph

A Gram-staining-negative, strictly aerobic bacterium, designated strain G3T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were moderately halotolerant and non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain G3T was observed between 15 and 40 °C (optimum 30 °C) and between pH 5.5 and 9.0 (optimum pH 6.5–7.5). Strain G3T contained Q-8 as the predominant lipoquinone and iso-C15 : 0, iso-C17 : 1ω9c, iso-C16 : 0 and iso-C11 : 0 as the major fatty acids. The G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain G3T formed a tight phylogenetic lineage with Luteimonas mephitis B1953/27.1T within the genus Luteimonas and was most closely related to L. mephitis B1953/27.1T with 98.0 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between strain G3T and L. mephitis B1953/27.1T was 35.2±3.3 %. On the basis of chemotaxonomic data and molecular properties, strain G3T represents a novel species of the genus Luteimonas, for which the name Luteimonas lutimaris sp. nov. is proposed. The type strain is G3T ( = KACC 14929T = JCM 16916T).

scholarly journals Marinobacterium lutimaris sp. nov., isolated from a tidal flat

2010 ◽  
Vol 60 (8) ◽  
pp. 1828-1831 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Se Hee Lee ◽  
Ji Young Jung ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Type Strain ◽  
Novel Species ◽  
Halophilic Bacterium ◽  
Molecular Data ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Gram Staining ◽  
Moderately Halophilic Bacterium ◽  
Optimum Ph

A Gram-staining-negative, moderately halophilic bacterium, designated strain AN9T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were catalase- and oxidase-positive short rods that were motile by means of a single polar flagellum. Growth of strain AN9T was observed at 15–40 °C (optimum, 25–30 °C) and at pH 6.0–8.0 (optimum, pH 6.5–7.5). Strain AN9T contained ubiquinone Q-8 as the predominant isoprenoid quinone and C10 : 0 3-OH (31.7 %), C18 : 1 ω7c (24.8 %), C16 : 0 (14.7 %) and summed feature 3 (comprising C16 : 1 ω7c and/or iso-C15 : 0 2-OH, 10.72 %) as the major fatty acids. The G+C content of the genomic DNA of strain AN9T was 58 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AN9T was related to members of the genus Marinobacterium and was related most closely to Marinobacterium litorale IMCC1877T (96.8 % similarity). On the basis of chemotaxonomic and molecular data, strain AN9T is considered to represent a novel species of the genus Marinobacterium, for which the name Marinobacterium lutimaris sp. nov. is proposed. The type strain is AN9T (=KACC 13703T =DSM 22012T).

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3835-3840 ◽  
Author(s):  
Eun Jin Choi ◽  
Hyo Jung Lee ◽  
Jeong Myeong Kim ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Gram Staining

A novel Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain 112T, was isolated from a tidal flat at Taean, Korea. Cells were catalase- and oxidase-positive ovoids to rods and did not produce bacteriochlorophyll a. Optimum growth of strain 112T was observed at 30 °C, at pH 6.5–7.5 and in the presence of 2–4 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 and Q-10 was detected as the predominant ubiquinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and three unidentified lipids. The genomic DNA G+C content of strain 112T was 58.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 112T formed a phyletic lineage within the genus Roseovarius of the class Alphaproteobacteria . Strain 112T was most closely related to Roseovarius mucosus DFL-24T, with 96.52 % similarity. On the basis of phenotypic, chemotaxonomic and molecular properties, strain 112T represents a novel species of the genus Roseovarius , for which the name Roseovarius lutimaris sp. nov. is proposed. The type strain is 112T ( = KACC 16185T = JCM 17743T).

scholarly journals Alteromonas naphthalenivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from tidal-flat sediment

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 4208-4214 ◽  
Author(s):  
Hyun Mi Jin ◽  
Kyung Hyun Kim ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Staining ◽  
Degrading Bacterium ◽  
Polycyclic Aromatic ◽  
Type Strains ◽  
Alteromonas Macleodii

A Gram-staining-negative and halotolerant bacterium, designated SN2T, capable of biodegrading polycyclic aromatic hydrocarbons, was isolated from a tidal flat contaminated with crude oil in Korea. Cells were strictly aerobic, catalase- and oxidase-positive, motile rods, with a single polar flagellum. Growth was observed at 4–37 °C (optimum, 25–30 °C) at pH 6.0–9.0 (optimum, pH 7.0–7.5) and in the presence of 0.5–9.0 % (w/v) NaCl (optimum, 2.0 %). Only ubiquinone 8 was detected as the isoprenoid quinone, and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1ω7c and C12 : 0 were observed as the major cellular fatty acids. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, a glycolipid, an aminolipid and three unidentified lipids. The DNA G+C content was 43.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SN2T formed a phylogenetic lineage with Alteromonas stellipolaris and Alteromonas addita within the genus Alteromonas, which was consistent with multilocus phylogenetic and MALDI-TOF MS analyses. Strain SN2T was most closely related to the type strains of A. stellipolaris, A. addita and Alteromonas macleodii, with 16S rRNA gene sequence similarities of 99.5, 99.3 and 98.4 % and DNA–DNA relatedness of 48.7 ± 6.6, 24.9 ± 7.5 and 27.9 ± 8.4 %, respectively. In conclusion, strain SN2T represents a novel species of the genus Alteromonas, for which the name Alteromonas naphthalenivorans sp. nov. is proposed. The type strain is SN2T ( = KCTC 11700BPT = JCM 17741T = KACC 18427T).

Gramella aestuarii sp. nov., isolated from a tidal flat, and emended description of Gramella echinicola

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2872-2878 ◽  
Author(s):  
Sang Hyeon Jeong ◽  
Hyun Mi Jin ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Molecular Features ◽  
Gram Staining ◽  
Emended Description

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterial strain motile by gliding, designated BS12T, was isolated from a tidal flat at Boseong, Korea. Cells were moderately halotolerant, catalase- and oxidase-positive rods. Growth was observed at 5–40 °C (optimum, 25 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–11 % (w/v) NaCl (optimum, 2–4 %). The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipid pattern comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 42.3 mol% and the only respiratory quinone was menaquinone 6. Phylogenetic inference based on 16S rRNA gene sequences showed that strain BS12T formed a tight phyletic lineage with members of the genus Gramella . Strain BS12T was most closely related to ‘ Gramella jeungdoensis’ HMD3159 with sequence similarity of 97.5 %, but the DNA–DNA relatedness value between the two strains was only 22.1±2.2 %. On the basis of phenotypic and molecular features, strain BS12T was shown to represent a novel species of the genus Gramella , for which the name Gramella aestuarii sp. nov. is proposed. The type strain is BS12T ( = KACC 16188T = JCM 17790T). An emended description of Gramella echinicola is also proposed.

scholarly journals Ramlibacter solisilvae sp. nov., isolated from forest soil, and emended description of the genus Ramlibacter

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1317-1322 ◽  
Author(s):  
Hyo Jung Lee ◽  
Se Hee Lee ◽  
Sang-Suk Lee ◽  
Joon Seon Lee ◽  
Yongseong Kim ◽  
...  
Keyword(s):  
Forest Soil ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Molecular Features ◽  
Gram Staining ◽  
Optimum Ph ◽  
Emended Description

A Gram-staining-negative, strictly aerobic, white-colony-forming bacterium, designated strain 5-10T, was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods or coccoids, showing oxidase- and catalase-positive reactions. Growth was observed at 10–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0–0.5 %). The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, C10 : 0 3-OH and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c). The G+C content of the genomic DNA was 69.9 mol% and the only respiratory quinone detected was ubiquinone 8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5-10T formed a tight phyletic lineage with members of the genus Ramlibacter . Strain 5-10T was most closely related to Ramlibacter tataouinensis TTB310T (97.3 %), but the DNA–DNA relatedness level between the two strains was 38.2±1.8 %. Based on phenotypic, chemotaxonomic and molecular features, strain 5-10T was shown to represent a novel species of the genus Ramlibacter , for which the name Ramlibacter solisilvae sp. nov. is proposed. The type strain is 5-10T ( = KACC 17567T = JCM 19319T). An emended description of the genus Ramlibacter is also proposed.

scholarly journals Chitinophaga ginsengihumi sp. nov., isolated from soil of ginseng rhizosphere

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2599-2604 ◽  
Author(s):  
Jae-Chan Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Polyphasic Analysis ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Optimum Ph

A novel strain designated SR18T was isolated from the rhizosphere soil of a ginseng in Korea. Cells were Gram-staining-negative, motile by gliding, catalase-positive and oxidase-negative, non-spore-forming rods. The isolate grew aerobically at 15–45 °C (optimum 28 °C), pH 5.5–7.5 (optimum pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum 1.5 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SR18T belongs to the genus Chitinophaga with sequence similarity of 97.2 % and 97.0 % to Chitinophaga japonensis 758T and Chitinophaga rupis CS5-B1T, respectively. Similarity to other species of the genus Chitinophaga was 92.8–95.5 %. The predominant menaquinone was MK-7. Major fatty acids were iso-C15 : 0 and C16 : 1ω5c. The polar lipids included phosphatidylethanolamine, unidentified phospholipids, unknown aminolipids and unknown lipids. The genomic DNA G+C content was 45.3 mol%. DNA–DNA relatedness between strain SR18T and C. japonensis NBRC 16041T was 29–32 %. On the basis of polyphasic analysis from this study, strain SR18T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga ginsengihumi sp. nov. is proposed. The type strain is SR18T ( = KACC 17604T = NBRC 109832T).

scholarly journals Belliella marina sp. nov., isolated from seawater

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4353-4357 ◽  
Author(s):  
Lei Song ◽  
Hongcan Liu ◽  
Jian Wang ◽  
Ying Huang ◽  
Xin Dai ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Optimum Ph ◽  
The Indian Ocean ◽  
Bacterium Strain

A Gram-stain-negative, rod-shaped bacterium, strain SW112T, was isolated from a seawater sample collected from the Indian Ocean. The strain was strictly aerobic and catalase- and oxidase-positive. Strain SW112T grew at 4–42 °C (optimum 30 °C), at pH 5.5–9.5 (optimum pH 7.5) and in the presence of 0–9.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant cellular fatty acids were iso-C15 : 0 (29.7 %), iso-C17 : 03-OH (14.3 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 15.1 %). The major menaquinone was menaquinone-7 and the major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain SW112T was 39 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SW112T was related to members of the genus Belliella, showing the highest similarity with Belliella aquatica TS-T86Tand Belliella baltica DSM 15883T (96.5 % and 96.4 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW112T represents a novel species of the genus Belliella, for which the name Belliella marina sp. nov. is proposed. The type strain is SW112T ( = CGMCC 1.15180T = KCTC 33694T).

scholarly journals Citreicella aestuarii sp. nov., isolated from a tidal flat

2011 ◽  
Vol 61 (11) ◽  
pp. 2595-2599 ◽  
Author(s):  
Moon Su Park ◽  
Bok-Sil Chung ◽  
Hyo Jung Lee ◽  
Hyun Mi Jin ◽  
Sang-Suk Lee ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Tidal Flat ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Optimum Ph

A Gram-negative bacterium, designated AD8T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8T was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8T was observed at 15–40 °C (optimum 25–30 °C), at pH 5.0–9.5 (optimum pH 6.0–7.5) and with 1.0–11.0 % (w/v) NaCl (optimum 2.0–4.0 %). The predominant fatty acids were C18 : 1ω7c and C19 : 0 cyclo ω8c. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8T belonged to the genus Citreicella and was most closely related to Citreicella marina CK-I3-6T within the class Alphaproteobacteria (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8T represents a novel species within the genus Citreicella, for which the name Citreicella aestuarii sp. nov. is proposed. The type strain is AD8T ( = KACC 13699T  = DSM 22011T).

scholarly journals Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4792-4799 ◽  
Author(s):  
Jae-Chan Lee ◽  
Young-Sook Kim ◽  
Bong-Sik Yun ◽  
Kyung-Sook Whang
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polyphasic Analysis ◽  
Optimum Ph ◽  
Unknown Phospholipid

A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103T, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103T were strictly aerobic, motile, straight rods and grew at pH 7.0–10.8 (optimum, pH 8.5), at 10–55 °C (optimum, 28 °C) and at salinities of 0–23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103T belongs to the genus Halomonas, showing highest sequence similarity to Halomonas boliviensis LC1T (97.7 %), Halomonas neptunia Eplume1T (97.7 %), Halomonas variabilis IIIT (97.7 %), Halomonas alkaliantarctica CRSST (97.7 %), Halomonas olivaria TYRC17T (97.5 %), Halomonas titanicae BH1T (97.2 %) and Halomonas sulfidaeris Esulfide1T (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were C18 : 1ω7c, C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C12 : 0 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA–DNA relatedness between strain BH103T and H. boliviensis KACC 16615T, H. neptunia KCTC 2888T, H. variabilis KCTC 2889T, H. alkaliantarctica KCTC 22844T, H. olivaria DSM 19074T, H. titanicae JCM 16411T and H. sulfidaeris DSM 15722T was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103T represents a novel species of the genus Halomonas, for which the name Halomonas salicampi sp. nov. is proposed. The type strain is BH103T ( = KACC 17609T = NBRC 109914T = NCAIM B 02528T).

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