scholarly journals Dietzia aerolata sp. nov., isolated from the air of a duck barn, and emended description of the genus Dietzia Rainey et al. 1995

2010 ◽  
Vol 60 (2) ◽  
pp. 393-396 ◽  
Author(s):  
P. Kämpfer ◽  
S. Langer ◽  
E. Martin ◽  
U. Jäckel ◽  
H.-J. Busse
Keyword(s):  
Polar Lipid ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Emended Description ◽  
Phosphatidylinositol Mannosides ◽  
Physiological And Biochemical

A Gram-stain-positive, coccoid, non-endospore-forming actinobacterium (Sj14aT) was isolated from the air of a duck barn on tryptone soy agar after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, strain Sj14aT was shown to belong to the genus Dietzia and was most closely related to Dietzia schimae (98.7 %), Dietzia cercidiphylli (98.4 %) and Dietzia maris (98.6 %). The major menaquinone was MK-8 (H2). The polar lipid profile included the major components diphosphatidylglycerol, phosphatidylglycerol, an unknown polar lipid and two unknown glycolipids. Phosphatidylinositol and two phosphatidylinositol mannosides, as well as several other unknown lipids, were also detected. The polyamine pattern contained the major compounds spermine and spermidine. The fatty acid profile, containing C16 : 0, C17 : 0, C18 : 1 ω9c and 10-methyl C18 : 0 as major fatty acids, supported the affiliation of strain Sj14aT to the genus Dietzia. The results of physiological and biochemical tests and DNA–DNA hybridizations allowed a clear phenotypic differentiation of strain Sj14aT from the most closely related species of the genus Dietzia. Strain Sj14aT represents a novel species, for which the name Dietzia aerolata sp. nov. is proposed, with the type strain Sj14aT (=DSM 45334T =CCM 7659T).

scholarly journals Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005

2010 ◽  
Vol 60 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Jenny Schäfer ◽  
Karin Martin ◽  
Peter Kämpfer
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Phenotypic Differentiation ◽  
Emended Description ◽  
Diamino Acid ◽  
Physiological And Biochemical ◽  
Sequence Similarities

A Gram-positive, coccoid-shaped organism (strain 02-Je-010T), forming yellow-pigmented colonies was isolated from the wall of an indoor environment. On the basis of 16S rRNA gene sequence similarity studies, it was shown that strain 02-Je-010T belongs to the genus Citricoccus with sequence similarities of 98.9 % to Citricoccus alkalitolerans DSM 15665T and 98.6 % to Citricoccus muralis DSM 14442T. Cell-wall sugars were mannose and glucose. The diagnostic diamino acid of the peptidoglycan was lysine. The major menaquinones detected were MK-9(H2) and MK-8(H2). The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and moderate amounts of two unknown phospholipids and two unknown glycolipids. The fatty acid profile comprised major amounts of anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. All these data supported the affiliation of strain 02-Je-010T to the genus Citricoccus. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 02-Je-010T from the two recognized Citricoccus species. For these reasons, strain 02-Je-010T represents a novel species, for which the name Citricoccus parietis sp. nov. is proposed, with the type strain 02-Je-010T (=CCUG 57388T=CCM 7609T).

scholarly journals Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli

2007 ◽  
Vol 57 (6) ◽  
pp. 1183-1187 ◽  
Author(s):  
Peter Kämpfer ◽  
Birgit Huber ◽  
Sandra Buczolits ◽  
Kathrin Thummes ◽  
Iris Grün-Wollny ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Polar Lipid ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Phenotypic Differentiation ◽  
Bacterium Strain ◽  
Phosphatidylinositol Mannosides

A Gram-positive, non-spore-forming bacterium (strain GW4-1778T) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778T is a member of the genus Nocardia, most closely related to Nocardia pseudobrasiliensis (GenBank accession no. DQ659914; 98.6 %), Nocardia nova (Z36930; 98.6 %), Nocardia niigatensis (AB092563; 98.4 %), Nocardia jiangxiensis (AY639902; 98.0 %), Nocardia uniformis (Z46752; 98.0 %) and Nocardia miyunensis (AY639901; 97.8 %). Strain GW4-1778T could be distinguished from any other established Nocardia species by sequence similarity values of less than 97.5 %. Strain GW4-1778T exhibited a quinone system with the predominant compound MK-8 (H4, ω-cycl) (99.5 %) and traces of MK-8 (H4), characteristic for the genus Nocardia. The polar lipid profile of strain GW4-1778T consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C15 : 0, C16 : 0, C17 : 1 ω8c and 10-methyl C17 : 0. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778T from related species with 16S rRNA gene similarities of >97.5 %. Therefore, strain GW4-1778T merits species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778T (=CCUG 53410T=CIP 109315T=DSM 45049T).

scholarly journals Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment

2010 ◽  
Vol 60 (4) ◽  
pp. 909-913 ◽  
Author(s):  
Peter Kämpfer ◽  
Jenny Schäfer ◽  
Nicole Lodders ◽  
Hans-Jürgen Busse
Keyword(s):  
Indoor Environment ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Diamino Acid ◽  
Type Strains

A Gram-stain-positive, rod-shaped, non-endospore-forming, orange-pigmented (coloured) actinobacterium (01-Je-003T) was isolated from the wall of an indoor environment primarily colonized with moulds. On the basis of 16S rRNA gene sequence similarity studies, strain 01-Je-003T was shown to belong to the genus Brevibacterium and was most similar to the type strains of Brevibacterium picturae (98.8 % similarity), Brevibacterium marinum (97.3 %) and Brevibacterium aurantiacum (97.2 %). Chemotaxonomic data [predominant quinone menaquinone MK-8(H2); polar lipid profile consisting of major compounds diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; polyamine pattern showing major compounds putrescine and cadaverine; major fatty acids anteiso-C15 : 0 and anteiso-C17 : 0] supported the affiliation of strain 01-Je-003T to the genus Brevibacterium. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 01-Je-003T from the two most closely related species, B. picturae and B. marinum. Strain 01-Je-003T therefore represents a novel species, for which the name Brevibacterium sandarakinum sp. nov. is proposed, with the type strain 01-Je-003T (=DSM 22082T =CCM 7649T).

scholarly journals Georgenia satyanarayanai sp. nov., an alkaliphilic and thermotolerant amylase-producing actinobacterium isolated from a soda lake

2012 ◽  
Vol 62 (Pt_10) ◽  
pp. 2405-2409 ◽  
Author(s):  
A. Srinivas ◽  
K. Rahul ◽  
Ch. Sasikala ◽  
Y. Subhash ◽  
E. V. V. Ramaprasad ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Soda Lake ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Content Type ◽  
Link Type ◽  
Alkaline Amylase ◽  
Phosphatidylinositol Mannosides ◽  
Physiological And Biochemical

A Gram-stain-positive, oxidase-negative, starch-hydrolysing, actinobacterium (strain JC82T) was isolated from a soda lake in Lonar, India. Based on 16S rRNA gene sequence similarity studies, strain JC82T belonged to the genus Georgenia and was most closely related to Georgenia muralis 1A-CT (96.8 %) and other members of the genus Georgenia (<96.5 %). The DNA G+C content of strain JC82T was 73.4 mol%. The cell-wall amino acids were alanine, glutamic acid and lysine with peptidoglycan type A4α. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid (L1) and an unidentified glycolipid (GL3). The predominant isoprenoid quinone was menaquinone MK-8(H4). Anteiso-C15 : 0 was the predominant fatty acid and significant proportions of iso-C14 : 0, C14 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 were also detected. Strain JC82T produced thermostable alkaline amylase. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain JC82T from all other members of the genus Georgenia . Based on these data, strain JC82T represents a novel species of the genus Georgenia , for which the name Georgenia satyanarayanai sp. nov. is proposed. The type strain is JC82T ( = KCTC 19802T = NBRC 107612T).

scholarly journals Actinophytocola burenkhanensis sp. nov., isolated from Mongolian soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1033-1038 ◽  
Author(s):  
Ismet Ara ◽  
Baljinova Tsetseg ◽  
Damdinsuren Daram ◽  
Manabu Suto ◽  
Katsuhiko Ando
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Actinomycete Strain ◽  
Physiological And Biochemical

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0203T, that formed pale yellow to orange-brown colonies and non-fragmented branched substrate mycelium is described. The strain, which produced very scanty aerial mycelium-like structures and scanty formation of spherical bodies on the aerial mycelium on Bennett’s agar medium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0203T grouped with the genus Actinophytocola, being most closely related to the type strain of Actinophytocola oryzae (97.8 %). Chemotaxonomic data [menaquinone MK-9(H4); iso-C16 : 0 (27 %), iso-C15 : 0 (18 %), C16 : 1 H (8 %), C16 : 0 9-methyl (8 %) as major fatty acids; glucose, galactose, ribose, arabinose, mannose, rhamnose and xylose (trace) as whole cell sugars; diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids as polar phospholipids] supported allocation of the strain to the genus Actinophytocola. Furthermore, the results of DNA–DNA hybridization of strain MN08-A0203T with the type strain of Actinophytocola oryzae revealed that the two strains were genetically distinct from each other. Moreover, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MN08-A0203T from closely related species. Thus, MN08-A0203T represents a novel species of the genus Actinophytocola, for which the name Actinophytocola burenkhanensis sp. nov. is proposed, with MN08-A0203T ( = NBRC 105883T  = VTCC D9-23T) as the type strain.

scholarly journals Jeotgalicoccus coquinae sp. nov. and Jeotgalicoccus aerolatus sp. nov., isolated from poultry houses

2011 ◽  
Vol 61 (2) ◽  
pp. 237-241 ◽  
Author(s):  
E. Martin ◽  
K. Klug ◽  
A. Frischmann ◽  
H.-J. Busse ◽  
P. Kämpfer ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Food Supplement ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Unidentified Phospholipid ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Poultry Houses

Two Gram-stain-positive, non-motile, non-spore-forming cocci (strains MK-7T and MPA-33T) were isolated from poultry houses. Strain MK-7T was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. Strain MPA-33T was isolated from the air of a turkey house on TSA after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, both strains were shown to belong to the genus Jeotgalicoccus; MK-7T was most closely related to Jeotgalicoccus psychrophilus YKJ-115T (99.3 % similarity) and MPA-33T was most closely related to Jeotgalicoccus halotolerans YKJ-101T (98.8 %). The quinone system of MK-7T was composed of equal amounts of menaquinones MK-7 and MK-6 and that of MPA-33T contained 76 % MK-7 and 24 % MK-6. The polar lipid profile of strain MK-7T consisted of the major compounds diphosphatidylglycerol and phosphatidylglycerol and six unidentified lipids present in minor to moderate amounts. In strain MPA-33T, diphosphatidylglycerol was the single predominant lipid, whereas phosphatidylglycerol was detected in moderate amounts. In addition, one unidentified phospholipid and four unidentified lipids were detected. Fatty acid profiles with iso-15 : 0 and anteiso-15 : 0 as major fatty acids supported the affiliation of the strains to the genus Jeotgalicoccus. The results of physiological and biochemical tests as well as DNA–DNA hybridizations allowed clear phenotypic differentiation of strains MK-7T and MPA-33T from the most closely related species. Strains MK-7T and MPA-33T therefore represent novel species, for which the names Jeotgalicoccus coquinae sp. nov. (type strain MK-7T =DSM 22419T =CCM 7682T =CCUG 57956T) and Jeotgalicoccus aerolatus sp. nov. (type strain MPA-33T =DSM 22420T =CCM 7679T =CCUG 57953T) are proposed.

scholarly journals Flavobacterium endophyticum sp. nov., a nifH gene-harbouring endophytic bacterium isolated from maize root

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3900-3904 ◽  
Author(s):  
Jun-lian Gao ◽  
Fan-yang Lv ◽  
Xu-ming Wang ◽  
Mei Yuan ◽  
Ji-wei Li ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Nifh Gene ◽  
Endophytic Bacterium ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Beijing China

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522T, was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus Flavobacterium and showed less than 93.9 % similarity to the type strains of all recognized species of the genus Flavobacterium. The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C15 : 0, iso-C15 : 0, iso-C15 : 1 G and iso-C16 : 0.The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522T from closely related species of the genus Flavobacterium. Strain 522T therefore represents a novel species within the genus Flavobacterium, for which the name Flavobacterium endophyticum sp. nov. is proposed. The type strain is 522T ( = ACCC 19708T = DSM 29537T).

scholarly journals Janibacter anophelis sp. nov., isolated from the midgut of Anopheles arabiensis

2006 ◽  
Vol 56 (2) ◽  
pp. 389-392 ◽  
Author(s):  
Peter Kämpfer ◽  
Olle Terenius ◽  
Jenny M. Lindh ◽  
Ingrid Faye
Keyword(s):  
Dna Hybridization ◽  
Anopheles Arabiensis ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Diamino Acid ◽  
Physiological And Biochemical

A Gram-positive, aerobic, non-motile strain, H2.16BT, isolated from the midgut of the mosquito Anopheles arabiensis was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain H2.16BT was shown to belong to the genus Janibacter, being most closely related to Janibacter melonis (98·3 %), Janibacter terrae (98·5 %) and Janibacter limosus (98·5 %). Chemotaxonomic data (meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall and major fatty acids of iso-C16 : 0, C17 : 1 ω8c and C17 : 0) supported the allocation of the strain to the genus Janibacter. The results of DNA–DNA hybridization and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain H2.16BT from closely related species. Thus, H2.16BT represents a novel species of the genus Janibacter, for which the name Janibacter anophelis sp. nov. is proposed. The type strain is H2.16BT (=CCUG 49715T=CIP 108728T).

scholarly journals Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments

2006 ◽  
Vol 56 (7) ◽  
pp. 1525-1529 ◽  
Author(s):  
Melissa M. Kendall ◽  
Yitai Liu ◽  
Magdalena Sieprawska-Lupa ◽  
Karl O. Stetter ◽  
William B. Whitman ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sediments ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Physiological And Biochemical

Three strains of CO2-reducing methanogens were isolated from marine sediments. Strain PL-15/HP was isolated from marine sediments of the Lipari Islands, near Sicily and the other two strains, Nankai-2 and Nankai-3T, were isolated from deep marine sediments of the Nankai Trough, about 50 km from the coast of Japan. Analysis of the cellular proteins and 16S rRNA gene sequences indicated that these three strains represented a single novel species that formed a deep branch of the mesophilic methanococci. Phylogenetic analysis indicated that the three strains were most closely related to Methanothermococcus okinawensis (95 % 16S rRNA gene sequence similarity). However, strains PL-15/HP, Nankai-2 and Nankai-3T grew at temperatures that were more similar to those of recognized species within the genus Methanococcus. Strain Nankai-3T grew fastest at 46 °C. Results of physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strains PL-15/HP, Nankai-2 and Nankai-3T from closely related species. The name Methanococcus aeolicus sp. nov. is proposed, with strain Nankai-3T (=OCM 812T=DSM 17508T) as the type strain.

Georgenia soli sp. nov., isolated from iron-ore-contaminated soil in India

2010 ◽  
Vol 60 (5) ◽  
pp. 1027-1030 ◽  
Author(s):  
P. Kämpfer ◽  
A. B. Arun ◽  
H.-J. Busse ◽  
S. Langer ◽  
C.-C. Young ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Iron Ore ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Polar Lipid Profile ◽  
Phenotypic Differentiation ◽  
Physiological And Biochemical

A Gram-positive actinobacterium (CC-NMPT-T3T) was isolated from iron-ore-contaminated soil near New Mangalore Port, Karnataka, India. Based on 16S rRNA gene sequence similarity studies, strain CC-NMPT-T3T belongs to the genus Georgenia and is most closely related to Georgenia muralis (98.6 %), Georgenia ruanii (97.4 %) and Georgenia thermotolerans (97.4 %). The peptidoglycan is of the type A4α l-Lys←l-Glu. The predominant isoprenoid quinone is menaquinone MK-8(H4) and the polar lipid profile is composed of the predominant compound diphosphatidylglycerol, moderate amounts of a phosphatidylinositol-mannoside, phosphatidylinositol and minor amounts of another phosphatidylmannoside and phosphatidylglycerol. The major fatty acids of strain CC-NMPT-T3T are anteiso-C15 : 0 and iso-C15 : 0. The results of DNA–DNA hybridizations, and physiological and biochemical tests allowed a clear phenotypic differentiation of strain CC-NMPT-T3T from all other Georgenia species. Strain CC-NMPT-T3T represents a novel species, for which the name Georgenia soli sp. nov. is proposed. The type strain is CC-NMPT-T3T (=DSM 21838T=CCM 7658T).

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