scholarly journals Marinicella litoralis gen. nov., sp. nov., a gammaproteobacterium isolated from coastal seawater

2010 ◽  
Vol 60 (7) ◽  
pp. 1613-1619 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova ◽  
Valery V. Mikhailov
Keyword(s):  
Sequence Similarity ◽  
Fatty Acid Analysis ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Coastal Seawater ◽  
Separate Branch ◽  
Marine Isolate ◽  
Novel Genus And Species ◽  
Bacterium Strain

A Gram-negative, aerobic, greyish–yellowish-pigmented, stenohaline, rod-shaped, non-motile bacterium, strain KMM 3900T, was isolated from a coastal seawater sample collected from the Sea of Japan. Based on phylogenetic analysis, strain KMM 3900T was positioned within the Gammaproteobacteria on a separate branch adjacent to members of the genera Reinekea and Kangiella, sharing less than 88 % 16S rRNA gene sequence similarity with all recognized species of the Gammaproteobacteria. The major isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown phospholipids. Fatty acid analysis revealed iso-C15 : 0, C16 : 1 ω7c and C16 : 0 as the dominant components. The DNA G+C content was 43.8 mol%. Based on its unique phenotypic characteristics and phylogenetic remoteness, marine isolate KMM 3900T is considered to represent a novel genus and species, for which the name Marinicella litoralis gen. nov., sp. nov. is proposed. The type strain of Marinicella litoralis is KMM 3900T (=NRIC 0758T =JCM 16154T).

scholarly journals Arenicella xantha gen. nov., sp. nov., a gammaproteobacterium isolated from a marine sandy sediment

2010 ◽  
Vol 60 (8) ◽  
pp. 1832-1836 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova ◽  
Valery V. Mikhailov
Keyword(s):  
Sequence Similarity ◽  
Phospholipid Fatty Acid ◽  
Fatty Acid Analysis ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
Phylogenetic Distance ◽  
Separate Branch ◽  
Novel Genus And Species ◽  
Bacterium Strain

A Gram-negative, aerobic, yellow-pigmented, rod-shaped, non-motile bacterium, strain KMM 3895T, was isolated from a marine sandy sample collected offshore from the Sea of Japan. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KMM 3895T in the class Gammaproteobacteria, forming a separate branch, sharing 89.5 % sequence similarity with Nitrincola lacisaponensis 4CAT and 88–87 % similarity with the other members of the cluster, including members of Kangiella, Spongiibacter, Alcanivorax and Microbulbifer. The major isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid and an unknown phospholipid. Fatty acid analysis revealed C16 : 1 ω7, iso-C16 : 0, iso-C18 : 0 and C18 : 1 ω7 as dominant components. The DNA G+C content was determined to be 48.1 mol%. Based on its unique phenotypic characteristics and phylogenetic distance, the marine coastal sediment isolate KMM 3895T should be classified as a representative of a novel genus and species, for which the name Arenicella xantha gen. nov., sp. nov. is proposed. The type strain of Arenicella xantha is KMM 3895T (=NRIC 0759T =JCM 16153T).

scholarly journals Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

2010 ◽  
Vol 60 (3) ◽  
pp. 603-609 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova
Keyword(s):  
Sea Of Japan ◽  
Sequence Similarity ◽  
Fatty Acid Analysis ◽  
Phenotypic Characterization ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Phylogenetic Distance ◽  
The Novel ◽  
Bacterial Strains ◽  
Separate Branch

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

scholarly journals Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea

2007 ◽  
Vol 57 (4) ◽  
pp. 764-769 ◽  
Author(s):  
Soon Dong Lee
Keyword(s):  
Marine Bacterium ◽  
Sequence Similarity ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Natural Seawater ◽  
Phylogenetic Distance ◽  
Phenotypic Characteristics ◽  
Beach Sediment ◽  
The Family ◽  
Novel Genus And Species

A Gram-negative, aerobic, saffron-coloured marine bacterium, designated HST1-43T, was isolated from beach sediment on the coast in Jeju, Korea, and its taxonomic status was established in a polyphasic study. 16S rRNA gene sequence analyses revealed that the isolate belonged to the family Flavobacteriaceae. The closest phylogenetic neighbours of strain HST1-43T were members of the genera Algibacter, Gaetbulibacter and Yeosuana, with levels of sequence similarity in the range 96.3–96.5 %. The isolate was non-motile and required sea salts or natural seawater for growth. The optimum temperature and pH ranges for growth were 25–30 °C and pH 6.1–8.1, respectively. MK-6 was the major menaquinone. The dominant cellular fatty acids were iso-C15 : 0, C18 : 0, C16 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The DNA G+C content was 36.2±0.4 mol%. On the basis of phylogenetic distance and phenotypic characteristics, the isolate is considered to represent a novel genus and species in the family Flavobacteriaceae, for which the name Tamlana crocina gen. nov., sp. nov. is proposed. The type strain is HST1-43T (=KCTC 12721T=JCM 14021T).

scholarly journals Cohnella thailandensis sp. nov., a xylanolytic bacterium from Thai soil

2010 ◽  
Vol 60 (10) ◽  
pp. 2284-2287 ◽  
Author(s):  
Saowapar Khianngam ◽  
Somboon Tanasupawat ◽  
Ancharida Akaracharanya ◽  
Kwang Kyu Kim ◽  
Keun Chul Lee ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Physiological And Biochemical Characteristics ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A xylanolytic bacterium, strain S1-3T, was isolated from soil collected in Nan province, Thailand. It was characterized taxonomically based on phenotypic characteristics and 16S rRNA gene sequence comparison. The strain was a Gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. Iso-C16 : 0 (39.5 %) and anteiso-C15 : 0 (26.8 %) were predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The DNA G+C content was 53.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain S1-3T was affiliated to the genus Cohnella, and was closely related to Cohnella ginsengisoli GR21-5T and Cohnella thermotolerans CCUG 47242T with 95.7 and 95.3 % sequence similarity, respectively. Strain S1-3T could be clearly distinguished from related species of the genus Cohnella by its physiological and biochemical characteristics as well as by its phylogenetic position. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella thailandensis sp. nov. is proposed. The type strain is S1-3T (=KCTC 22296T =TISTR 1890T =PCU 306T).

scholarly journals Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum ‘Verrucomicrobia’, isolated from a sponge (Porifera)

2006 ◽  
Vol 56 (9) ◽  
pp. 2119-2124 ◽  
Author(s):  
Matthias Scheuermayer ◽  
Tobias A. M. Gulder ◽  
Gerhard Bringmann ◽  
Ute Hentschel
Keyword(s):  
Genomic Dna ◽  
Type Strain ◽  
Marine Bacterium ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Ph Values ◽  
Novel Genus And Species ◽  
Bacterium Strain

A marine bacterium, strain Pol012T, was isolated from the Mediterranean sponge Axinella polypoides and subsequently characterized as belonging to subphylum 1 of the phylum ‘Verrucomicrobia’. Strain Pol012T was non-motile, Gram-negative, coccoid or rod-shaped and red in colour. The menaquinones MK-8 and MK-9 were detected. The G+C content of the genomic DNA was 50.9 mol%. Growth was possible at temperatures between 8 and 30 °C and at pH values between 6.8 and 8.2. The closest cultured relative of strain Pol012T was Akkermansia muciniphila (83 % sequence similarity), while the closest environmental 16S rRNA gene sequence was the marine clone Arctic96BD-2 (95 % sequence similarity). Strain Pol012T is the first marine pure-culture representative of ‘Verrucomicrobia’ subphylum 1 and represents a novel genus and species, for which the name Rubritalea marina gen. nov., sp. nov. is proposed. The type strain is Pol012T (=DSM 177716T=CIP 108984T).

scholarly journals Simiduia litorea sp. nov., isolated from seashore sediments of the Sea of Japan

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2688-2692 ◽  
Author(s):  
Naoto Tanaka ◽  
Lyudmila A. Romanenko ◽  
Vassilii I. Svetashev ◽  
Valery V. Mikhailov
Keyword(s):  
Sea Of Japan ◽  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis

An aerobic, Gram-stain-negative, agarolytic rod-shaped bacterium, designated KMM 9504T, was isolated from a sediment sample collected from the seashore of the Sea of Japan. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9504T belonged to the genus Simiduia as it was most closely related to Simiduia areninigrae KCTC 23293T (97.3 % sequence similarity). Strain KMM 9504T was characterized by the major ubiquinone Q-8, and by the predominance of C16 : 1ω7c, C17 : 1ω8c, followed by C16 : 0, C15 : 0, C17 : 0 and C12 : 1 in its fatty acid profile. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid, an unknown aminolipid, unknown phospholipids, and unknown lipids. Based on the distinctive phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results, it is concluded that strain KMM 9504T represents a novel species of the genus Simiduia , for which the name Simiduia litorea sp. nov. is proposed. The type strain of the species is strain KMM 9504T ( = NRIC 0917T = JCM 19759T).

scholarly journals Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star

2007 ◽  
Vol 57 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Masataka Uchino ◽  
Galina M. Frolova ◽  
Valery V. Mikhailov
Keyword(s):  
Deep Sea ◽  
Marine Bacterium ◽  
Sequence Similarity ◽  
Brittle Star ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Distinct Evolutionary Lineage ◽  
The Family ◽  
Novel Genus And Species ◽  
Bacterium Strain

An aerobic, Gram-negative, non-motile, yellow-pigmented bacterium, strain KMM 3046T, was isolated from a deep-sea brittle star from the Fiji Sea and was subjected to a polyphasic taxonomic analysis. Strain KMM 3046T grew at 5–32 °C and in the presence of 1–12 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and 3-OH i16 : 0, 3-OH i17 : 0 and 3-OH a17 : 0 as the major fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KMM 3046T forms a distinct evolutionary lineage within the family Flavobacteriaceae (phylum Bacteroidetes), displaying 92.3–91.9 % sequence similarity with respect to Salegentibacter species. On the basis of the phenotypic and phylogenetic data, strain KMM 3046T represents a novel genus and species of the family Flavobacteriaceae, for which the name Marixanthomonas ophiurae gen. nov., sp. nov. is proposed. The type strain of Marixanthomonas ophiurae is KMM 3046T (=NRIC 0684T=JCM 14121T).

scholarly journals Cocleimonas flava gen. nov., sp. nov., a gammaproteobacterium isolated from sand snail (Umbonium costatum)

2011 ◽  
Vol 61 (2) ◽  
pp. 412-416 ◽  
Author(s):  
Naoto Tanaka ◽  
Lyudmila A. Romanenko ◽  
Takao Iino ◽  
Galina M. Frolova ◽  
Valery V. Mikhailov
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Novel Genus And Species ◽  
Unknown Phospholipid ◽  
Sulfur Oxidizing ◽  
Distinct Phylogenetic Lineage

A Gram-stain-negative, aerobic, yellow-pigmented, heterotrophic, sulfur-oxidizing, non-motile, rod-shaped bacterium, designated KMM 3898T, was isolated from an internal tissue of the sand snail Umbonium costatum, collected from the shallow sediments of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KMM 3898T formed a distinct phylogenetic lineage within the class Gammaproteobacteria and was most closely related to Leucothrix mucor DSM 2157T (89.2 % 16S rRNA gene sequence similarity) and members of the genus Thiothrix (86.7–88.5 %). Chemotaxonomically, strain KMM 3898T contained the isoprenoid quinone Q-8, the polar lipids phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid and the fatty acids C18 : 1 ω7c, C16 : 1 ω7c and C16 : 0 as predominant components (>10 %). The DNA G+C content of strain KMM 3898T was 43.4 mol%. On the basis of phenotypic features and phylogenetic analysis, strain KMM 3898T represents a novel genus and species, for which the name Cocleimonas flava gen. nov., sp. nov. is proposed. The type strain is KMM 3898T (=NRIC 0757T =JCM 16494T).

Luteimonas vadosa sp. nov., isolated from seashore sediment

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1261-1266 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Vassilii I. Svetashev ◽  
Valeriya V. Kurilenko ◽  
Valery V. Mikhailov
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Organic Substrates ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis

An aerobic, Gram-stain-negative, yellow-pigmented, non-motile, rod-shaped bacterium designated strain KMM 9005T was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9005T belonged to the genus Luteimonas and was most closely related to Luteimonas cucumeris KCTC 23627T (96.5 % sequence similarity) and Luteimonas aquatica LMG 24212T (96.1 % sequence similarity). Strain KMM 9005T was characterized by the presence of thin fimbriae, the major ubiquinone Q-8, by the predominance of iso-C17 : 1 followed by iso-C16 : 0, iso-C15 : 0 and iso-C17 : 0 in its fatty acid profile, weak hydrolytic capacity and the inability to assimilate most organic substrates. Based on these distinctive phenotypic characteristics and phylogenetic analysis, strain KMM 9005T represents a novel species of the genus Luteimonas , for which the name Luteimonas vadosa sp. nov. is proposed. The type strain is KMM 9005T ( = NRIC 0881T = JCM 18392T).

scholarly journals Aestuariibacter litoralis sp. nov., isolated from a sandy sediment of the Sea of Japan

2010 ◽  
Vol 60 (2) ◽  
pp. 317-320 ◽  
Author(s):  
Naoto Tanaka ◽  
Lyudmila A. Romanenko ◽  
Galina M. Frolova ◽  
Valery V. Mikhailov
Keyword(s):  
Sea Of Japan ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Fatty Acid Analysis ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis

The phenotypic and phylogenetic characteristics of an aerobic, Gram-negative, motile, non-pigmented Alteromonas-like bacterium (designated strain KMM 3894T), isolated from a sandy sediment sample collected offshore of the Sea of Japan, were investigated. Comparative 16S rRNA gene sequence analysis revealed that strain KMM 3894T belonged to the genus Aestuariibacter and was most closely related to Aestuariibacter halophilus JC2043T (95.5 % sequence similarity). Fatty acid analysis showed C16 : 1 ω7c, C18 : 1 ω7c, and C16 : 0 as the dominant components. Strain KMM 3894T could be differentiated from recognized species of the genus Aestuariibacter by its ability to grow at 4 °C and at 30 °C, the optimum temperature for growth, and its inability to utilize most carbohydrates. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain KMM 3894T is considered to represent a novel species of the genus Aestuariibacter, for which the name Aestuariibacter litoralis sp. nov. is proposed. The type strain is KMM 3894T (=NRIC 0754T=JCM 15896T).

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