scholarly journals Agromyces bauzanensis sp. nov., isolated from soil

2010 ◽  
Vol 60 (10) ◽  
pp. 2341-2345 ◽  
Author(s):  
De-Chao Zhang ◽  
Peter Schumann ◽  
Hong-Can Liu ◽  
Yu-Hua Xin ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Cell Wall ◽  
Type Strain ◽  
Novel Species ◽  
Molar Ratio ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Genotypic Analysis

A Gram-stain-positive, aerobic bacterium, designated strain BZ41T, was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41T was related to members of the genus Agromyces and showed highest similarity with the type strain of Agromyces ramosus (96.8 %). The morphological, biochemical and chemotaxonomic characteristics of the new isolate were consistent with the description of the genus Agromyces. The cell-wall peptidoglycan of strain BZ41T was of type B2γ and contained the amino acids 2,4-diaminobutyric acid, alanine, glycine and glutamic acid in an approximate molar ratio of 1.8 : 0.7 : 1.1 : 1.0. The predominant cell-wall sugars were galactose, glucose, mannose and rhamnose. Strain BZ41T had MK-12 and MK-11 as major menaquinones and contained anteiso-C15 : 0 and anteiso-C17 : 0 as major fatty acids. The genomic DNA G+C content of strain BZ41T was 69.7 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ41T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces bauzanensis sp. nov. is proposed. The type strain is BZ41T (=DSM 22275T =CGMCC 1.8984T).

scholarly journals Hymenobacter wooponensis sp. nov., isolated from wetland freshwater

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1871-1876 ◽  
Author(s):  
Joo Won Kang ◽  
Ji Hee Lee ◽  
Keun Sik Baik ◽  
Sang Suk Lee ◽  
Chi Nam Seong
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Nearest Neighbour ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Study Support

A non-motile and rod-shaped bacterium, designated strain WM78T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 :0, anteiso-C15 :0, C16 :1ω7c and/or C16 :1ω6c and iso-C17 :1 I and/or anteiso-C17 :1 B. The strain contained MK-7 as the major isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The genomic DNA G+C content was 62 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WM78T forms evolutionary lineage within the radiation comprising members of the genus Hymenobacter with Hymenobacter gelipurpurascens Txg1T (97.7 % sequence similarity) as its nearest neighbour. A number of phenotypic characteristics distinguished strain WM78T from related members of the genus Hymenobacter. The evidence presented in this study support the designation of strain WM78T as a representative of a novel species in the genus Hymenobacter for which the name Hymenbactor wooponensis sp. nov. is proposed. The type strain is WM78T ( = KCTC 32528T = JCM 19491T).

scholarly journals Bacillus taiwanensis sp. nov., isolated from a soil sample from Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2078-2084 ◽  
Author(s):  
Bo Liu ◽  
Guo-Hong Liu ◽  
Cetin Sengonca ◽  
Peter Schumann ◽  
Ming-Kuang Wang ◽  
...  
Keyword(s):  
Soil Sample ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Optimum Ph ◽  
Diamino Acid

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-14571T) was isolated from a soil sample in Taiwan. Strain FJAT-14571T grew at 20–40 °C (optimum 35 °C), pH 6–10 (optimum pH 8) and 0–2 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-14571T was a member of the genus Bacillus and was most closely related to Bacillus oceanisediminis DSM 24771T (96.2 %). DNA–DNA relatedness between strain FJAT-14571T and B. oceanisediminis DSM 24771T was low (32.0 % ± 0.88 %). The diagnostic diamino acid of the peptidoglycan of strain FJAT-14571T was meso-diaminopimelic acid and the predominant menaquinone was MK-7 (96.6 %). The major cellular fatty acids were iso-C15 : 0 (46.4 %), anteiso-C15 : 0 (7.6 %), iso-C17 : 0 (8.2 %) and iso-C16 : 0 (10.0 %) and the DNA G+C content was 40.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain FJAT-14571T represents a novel species within the genus Bacillus, for which the name Bacillus taiwanensis sp. nov. is proposed. The type strain is FJAT-14571T ( = DSM 27845T = CGMCC1.1 2698T).

scholarly journals Carboxylicivirga linearis sp. nov., isolated from a sea cucumber culture pond

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3271-3275 ◽  
Author(s):  
Feng-Qing Wang ◽  
Yan-Xia Zhou ◽  
Xue-Zheng Lin ◽  
Guan-Jun Chen ◽  
Zong-Jun Du
Keyword(s):  
Sea Cucumber ◽  
Novel Species ◽  
Optimal Growth ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Respiratory Quinone ◽  
Emended Description

A yellow-pigmented, Gram-stain-negative and facultatively anaerobic bacterium, designated FB218T, was isolated from a sediment sample collected from a sea cucumber culture pond in Rongcheng, China (36° 54′ 36″ N 122° 14′ 34″ E). Cells of strain FB218T were slender, gliding, catalase-positive and oxidase-negative. Optimal growth occurred at 30 °C, pH 6.5–7.0 and in medium containing 2–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FB218T belonged to the genus Carboxylicivirga, family Marinilabiliaceae. The predominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. MK-7 was the main respiratory quinone. The major polar lipids of strain FB218T were two unidentified lipids and a phospholipid. The genomic DNA G+C content was 40.0 mol%. Based on the distinct phylogenetic position and the combination of physiological and phenotypic characteristics, strain FB218T represents a novel species of the genus Carboxylicivirga, for which the name Carboxylicivirga linearis sp. nov. is proposed. The type strain is FB218T ( = KCTC 42254T = MCCC 1H00106T). An emended description of the genus Carboxylicivirga is also provided.

Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite

2012 ◽  
Vol 62 (2) ◽  
pp. 397-402 ◽  
Author(s):  
Rosa Margesin ◽  
Peter Schumann ◽  
De-Chao Zhang ◽  
Mersiha Redzic ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Alpine Glacier ◽  
Cellular Fatty Acids

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1–25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys–l-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0 and iso-C15 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T ( = DSM 23324T  = LMG 26052T  = CGMCC 1.10698T).

Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 66-71 ◽  
Author(s):  
Jin-Jin Liu ◽  
Xin-Qi Zhang ◽  
Fang-Tao Chi ◽  
Jie Pan ◽  
Cong Sun ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Sea Salts ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, non-motile and aerobic bacterium, designated CF17T, was isolated from coastal planktonic seaweeds, East China Sea. The isolate grew at 18–37 °C (optimum 25–28 °C), pH 6.5–9.0 (optimum 7.0–8.0) and with 0–5 % NaCl (optimum 1–2 %, w/v) and 0.5–10 % sea salts (optimum 2–3 %, w/v). Growth of strain CF17T could be stimulated prominently by supplementing the growth medium with the autoclaved supernatant of a culture of strain CF5, which was isolated from the same sample along with strain CF17T. The cell morphology of strain CF17T was a bean-shaped rod consisting of a swollen end and a long prostheca. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CF17T clustered with Gemmobacter nectariphilus DSM 15620T within the genus Gemmobacter . The DNA G+C content of strain CF17T was 61.4 mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids included C18 : 1ω7c and C18 : 0. The polar lipids of strain CF17T consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, one uncharacterized aminolipid, three uncharacterized glycolipids and one uncharacterized lipid. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CF17T ( = CGMCC 1.11024T = JCM 18498T) is considered to represent a novel species of the genus Gemmobacter , for which the name Gemmobacter megaterium sp. nov. is proposed.

scholarly journals Chryseobacterium flavum sp. nov., isolated from polluted soil

2007 ◽  
Vol 57 (8) ◽  
pp. 1765-1769 ◽  
Author(s):  
Yu Zhou ◽  
Jing Dong ◽  
Xu Wang ◽  
Xing Huang ◽  
Ke-Yun Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Novel Species ◽  
Polluted Soil ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Gram Negative ◽  
Genotypic Characteristics

A Gram-negative, non-motile, rod-shaped bacterial strain, designated CW-E 2T, was isolated from a polluted soil sample collected from Jiangsu Province, China. A taxonomic study of the isolate, including phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics, was carried out. The predominant menaquinone was MK-6 and the major fatty acids were i-C15 : 0, i-C17 : 0 3-OH, i-C17 : 1 ω9c and summed feature 4. The G+C content of the DNA was 37.2 mol%. Based on phenotypic and genotypic characteristics, strain CW-E 2T represents a novel species of the genus Chryseobacterium for which the name Chryseobacterium flavum sp. nov. is proposed. The type strain is CW-E 2T (=KCTC 12877T=CCTCC AB 206147T).

scholarly journals Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4038-4042 ◽  
Author(s):  
Keun Sik Baik ◽  
Mi Sun Kim ◽  
Ji Hee Lee ◽  
Sang Suk Lee ◽  
Wan-Taek Im ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A non-motile and rod-shaped bacterium, designated strain 02SUJ3T, was isolated from freshwater collected from the Juam Reservoir (Republic of Korea). Cells were Gram-stain-negative, aerobic, oxidase-negative and catalase-positive. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The strain contained MK-7 as the major isoprenoid quinone. The main polar lipid was phosphatidylethanolamine. The DNA G+C content was 46.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 02SUJ3T forms an independent lineage within the genus Flavisolibacter with low sequence similarity to Flavisolibacter ginsengiterrae Gsoil 492T and Flavisolibacter ginsengisoli Gsoil 643T (95.7 %). Phenotypic characteristics distinguished strain 02SUJ3T from members of the genus Flavisolibacter . On the basis of the evidence presented in this study, strain 02SUJ3T is considered to represent a novel species, for which the name Flavisolibacter rigui sp. nov. is proposed. The type strain is 02SUJ3T ( = JCM 17515T = KCTC 23328T). An emended description of the genus Flavisolibacter is also provided.

scholarly journals Sphingosinicella vermicomposti sp. nov., isolated from vermicompost, and emended description of the genus Sphingosinicella

2010 ◽  
Vol 60 (3) ◽  
pp. 580-584 ◽  
Author(s):  
Muhammad Yasir ◽  
Zubair Aslam ◽  
Geun Cheol Song ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
The Family ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Total Dna

A Gram-stain-negative, rod-shaped bacterium, designated strain YC7378T was isolated from vermicompost (VC) collected at Masan, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC7378T grew optimally at 30 °C and at pH 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC7378T belongs to the genus Sphingosinicella in the family Sphingomonadaceae. The most closely related strains are Sphingosinicella soli KSL-125T (95.7 %), Sphingosinicella xenopeptidilytica 3-2W4T (95.6 %) and Sphingosinicella microcystinivorans Y2T (95.5 %). Strain YC7378T contained ubiquinone Q-10 as the major respiratory quinone system and sym-homospermidine as the major polyamine. The major fatty acids of strain YC7378T were C18 : 1 ω7c, C16 : 1 ω7c and/or iso-C15 : 0 2-OH, C14 : 0 2-OH and C16 : 0. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The total DNA G+C content was 59.4 mol%. The phenotypic, phylogenetic and chemotaxonomic data showed that strain YC7378T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella vermicomposti sp. nov. is proposed. The type strain is YC7378T (=KCTC 22446T =DSM 21593T).

scholarly journals Paenibacillus hunanensis sp. nov., isolated from rice seeds

2010 ◽  
Vol 60 (6) ◽  
pp. 1266-1270 ◽  
Author(s):  
Yang Liu ◽  
Lin Liu ◽  
Fubin Qiu ◽  
Peter Schumann ◽  
Yu Shi ◽  
...  
Keyword(s):  
Type Strain ◽  
Hybrid Rice ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids

Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05T, FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05T was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C15 : 0 (61.7 %), C16 : 0 (10.9 %), iso-C16 : 0 (7.0 %), anteiso-C17 : 0 (6.7 %) and iso-C15 : 0 (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05T (=ACCC 10718T =CGMCC 1.8907T =DSM 22170T).

scholarly journals Carboxylicivirga marinus sp. nov., Isolated From Marine Sediment, and Genome Insight of the Genus Carboxylicivirga

2021 ◽  
Vol 8 ◽  
Author(s):  
Tian-Shu Zhang ◽  
Ya-Ning Zhang ◽  
De-Chen Lu ◽  
Ya Gong ◽  
Zong-Jun Du
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Average Nucleotide Identity ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
The 16S Rrna Gene ◽  
Optimal Ph

A Gram-stain-negative, facultatively aerobic, beige and slender bacterium, designed N1Y132T, was isolated from a sediment sample taken from coastal zone of Weihai, China (37°34′31.44″ N, 122°9′15″ E). Growth occurred at 15-33°C (optimal 25–28°C), at pH 6.5–8.5 (optimal pH 7.5), in 0–9% (w/v) NaCl (optimal 2.0–2.5%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain N1Y132T showed the highest sequence similarity (97.9%) with Carboxylicivirga taeanensis JCM 19490T, followed by C. sediminis JR1T (97.4%), C. mesophila JCM 18290T (96.4%) and C. linearis FB218T (95.1%). Thus, strain N1Y132T was a member of the genus Carboxylicivirga. The average nucleotide identity (ANI) values between strain N1Y132T and C. sediminis JR1T, C. linearis FB218T and C. mesophila JCM 18290T were 74.8%, 71.1%, and 74.9%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was MK-7 and the major fatty acids (> 5.0%) included iso-C15:0, anteiso-C15:0, iso-C13:0, iso-C14:0, iso-C15:0 3-OH, and C15:1ω6c. The polar lipids were consisted of a phosphatidylethanolamine, two phosphoaminolipids and six unidentified lipids. The DNA G + C content was 38.1 mol%. Based on the evidence presented in this study, strain N1Y132T represents a novel species of the genus Carboxylicivirga, for which the name Carboxylicivirga marinus sp. nov. is proposed. The type strain is N1Y132T (= KCTC 72934T = MCCC 1H00431T).

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