scholarly journals Paenibacillus hunanensis sp. nov., isolated from rice seeds

2010 ◽  
Vol 60 (6) ◽  
pp. 1266-1270 ◽  
Author(s):  
Yang Liu ◽  
Lin Liu ◽  
Fubin Qiu ◽  
Peter Schumann ◽  
Yu Shi ◽  
...  
Keyword(s):  
Type Strain ◽  
Hybrid Rice ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids

Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05T, FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05T was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C15 : 0 (61.7 %), C16 : 0 (10.9 %), iso-C16 : 0 (7.0 %), anteiso-C17 : 0 (6.7 %) and iso-C15 : 0 (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05T (=ACCC 10718T =CGMCC 1.8907T =DSM 22170T).

scholarly journals Carboxylicivirga marinus sp. nov., Isolated From Marine Sediment, and Genome Insight of the Genus Carboxylicivirga

2021 ◽  
Vol 8 ◽  
Author(s):  
Tian-Shu Zhang ◽  
Ya-Ning Zhang ◽  
De-Chen Lu ◽  
Ya Gong ◽  
Zong-Jun Du
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Average Nucleotide Identity ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
The 16S Rrna Gene ◽  
Optimal Ph

A Gram-stain-negative, facultatively aerobic, beige and slender bacterium, designed N1Y132T, was isolated from a sediment sample taken from coastal zone of Weihai, China (37°34′31.44″ N, 122°9′15″ E). Growth occurred at 15-33°C (optimal 25–28°C), at pH 6.5–8.5 (optimal pH 7.5), in 0–9% (w/v) NaCl (optimal 2.0–2.5%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain N1Y132T showed the highest sequence similarity (97.9%) with Carboxylicivirga taeanensis JCM 19490T, followed by C. sediminis JR1T (97.4%), C. mesophila JCM 18290T (96.4%) and C. linearis FB218T (95.1%). Thus, strain N1Y132T was a member of the genus Carboxylicivirga. The average nucleotide identity (ANI) values between strain N1Y132T and C. sediminis JR1T, C. linearis FB218T and C. mesophila JCM 18290T were 74.8%, 71.1%, and 74.9%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was MK-7 and the major fatty acids (> 5.0%) included iso-C15:0, anteiso-C15:0, iso-C13:0, iso-C14:0, iso-C15:0 3-OH, and C15:1ω6c. The polar lipids were consisted of a phosphatidylethanolamine, two phosphoaminolipids and six unidentified lipids. The DNA G + C content was 38.1 mol%. Based on the evidence presented in this study, strain N1Y132T represents a novel species of the genus Carboxylicivirga, for which the name Carboxylicivirga marinus sp. nov. is proposed. The type strain is N1Y132T (= KCTC 72934T = MCCC 1H00431T).

scholarly journals Primorskyibacter insulae sp. nov., isolated from the junction between the ocean and a freshwater spring

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3971-3976 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Yong-Taek Jung ◽  
Sung-Min Won ◽  
Jung-Hoon Yoon
Keyword(s):  
Type Strain ◽  
Phylogenetic Trees ◽  
Novel Species ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Freshwater Spring

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped bacterial strain, designated SSK3-2T, was isolated from the locality where the ocean and a freshwater spring meet at Jeju island, South Korea, and subjected to a polyphasic taxonomic study. Strain SSK3-2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK3-2T clustered with the type strain of Primorskyibacter sedentarius, with which it exhibited 97.3 % sequence similarity. Strain SSK3-2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain SSK3-2T were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain SSK3-2T was 60.6 mol% and its mean DNA–DNA relatedness value with P. sedentarius JCM 16874T was 19 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK3-2T is separated from P. sedentarius. On the basis of the data presented, strain SSK3-2T is considered to represent a novel species of the genus Primorskyibacter, for which the name Primorskyibacter insulae sp. nov. is proposed. The type strain is SSK3-2T ( = KCTC 42602T = CECT 8871T).

Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite

2012 ◽  
Vol 62 (2) ◽  
pp. 397-402 ◽  
Author(s):  
Rosa Margesin ◽  
Peter Schumann ◽  
De-Chao Zhang ◽  
Mersiha Redzic ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Alpine Glacier ◽  
Cellular Fatty Acids

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1–25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys–l-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0 and iso-C15 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T ( = DSM 23324T  = LMG 26052T  = CGMCC 1.10698T).

scholarly journals Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field

2011 ◽  
Vol 61 (7) ◽  
pp. 1715-1719 ◽  
Author(s):  
Sang-Hoon Baek ◽  
Yingshun Cui ◽  
Sun-Chang Kim ◽  
Chang-Hao Cui ◽  
Chengri Yin ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A Gram-reaction-positive, rod-shaped, spore-forming bacterium, designated Gsoil 1105T, was isolated from soil of a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the order Bacillales, showing the highest level of sequence similarity with respect to Tumebacillus permanentifrigoris Eur1 9.5T (94.6 %). The phylogenetic distances from other described species with validly published names within the order Bacillales were greater than 9.0 %. Strain Gsoil 1105T had a genomic DNA G+C content of 55.6 mol% and menaquinone 7 (MK-7) as the major respiratory quinone. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1105T represents a novel species of the genus Tumebacillus, for which the name Tumebacillus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 1105T ( = KCTC 13942T  = DSM 18389T).

scholarly journals Sphingosinicella vermicomposti sp. nov., isolated from vermicompost, and emended description of the genus Sphingosinicella

2010 ◽  
Vol 60 (3) ◽  
pp. 580-584 ◽  
Author(s):  
Muhammad Yasir ◽  
Zubair Aslam ◽  
Geun Cheol Song ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
The Family ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Total Dna

A Gram-stain-negative, rod-shaped bacterium, designated strain YC7378T was isolated from vermicompost (VC) collected at Masan, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC7378T grew optimally at 30 °C and at pH 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC7378T belongs to the genus Sphingosinicella in the family Sphingomonadaceae. The most closely related strains are Sphingosinicella soli KSL-125T (95.7 %), Sphingosinicella xenopeptidilytica 3-2W4T (95.6 %) and Sphingosinicella microcystinivorans Y2T (95.5 %). Strain YC7378T contained ubiquinone Q-10 as the major respiratory quinone system and sym-homospermidine as the major polyamine. The major fatty acids of strain YC7378T were C18 : 1 ω7c, C16 : 1 ω7c and/or iso-C15 : 0 2-OH, C14 : 0 2-OH and C16 : 0. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The total DNA G+C content was 59.4 mol%. The phenotypic, phylogenetic and chemotaxonomic data showed that strain YC7378T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella vermicomposti sp. nov. is proposed. The type strain is YC7378T (=KCTC 22446T =DSM 21593T).

scholarly journals Tenacibaculum geojense sp. nov., isolated from seawater

2012 ◽  
Vol 62 (1) ◽  
pp. 18-22 ◽  
Author(s):  
So-Jung Kang ◽  
Soo-Young Lee ◽  
Mi-Hwa Lee ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Motile By Gliding

A Gram-negative, non-flagellated, non-spore-forming bacterium, designated YCS-6T, that was motile by gliding, was isolated from seawater on the southern coast of Korea. Strain YCS-6T grew optimally at 30 °C and with 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YCS-6T fell within the genus Tenacibaculum and was most closely associated with Tenacibaculum litopenaei B-IT, with which the isolate exhibited 95.9 % 16S rRNA gene sequence similarity. Sequence similarity between strain YCS-6T and other members of the genus Tenacibaculum was 93.8–95.7 %. Strain YCS-6T contained menaquinone-6 (MK-6) as the predominant respiratory quinone and iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 0 3-OH and iso-C15 : 1 G as the major fatty acids. The DNA G+C content was 32.7 mol%. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain YCS-6T from all other members of the genus Tenacibaculum. On the basis of our phenotypic, chemotaxonomic and phylogenetic data, strain YCS-6T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum geojense sp. nov. is proposed. The type strain is YCS-6T ( = KCTC 23423T  = CCUG 60527T).

scholarly journals Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field

2006 ◽  
Vol 56 (12) ◽  
pp. 2861-2866 ◽  
Author(s):  
Leonid N. Ten ◽  
Sang-Hoon Baek ◽  
Wan-Taek Im ◽  
Qing-Mei Liu ◽  
Zubair Aslam ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
Distinct Phylogenetic Lineage

A Gram-positive, non-motile, endospore-forming bacterium, designated Gsoil 1517T, was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized in order to determine its taxonomic position, using a polyphasic approach. It was found to rod-shaped and aerobic or facultatively anaerobic. It grew optimally at 30 °C and at pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1517T forms a distinct phylogenetic lineage within the genus Bacillus, being related to Bacillus funiculus JCM 11201T (96.8 %). The strain showed less than 94.3 % sequence similarity with other Bacillus species. The G+C content of the genomic DNA was found to be 47.8 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C15 : 0 (42.4 %), anteiso-C15 : 0 (17.4 %), iso-C14 : 0 (9.7 %) and C16 : 0 (6.0 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1517T represents a novel species of the genus Bacillus, for which the name Bacillus panaciterrae sp. nov. is proposed. The type strain is Gsoil 1517T (=KCTC 13929T=CCUG 52470T=LMG 23408T).

scholarly journals Frateuria terrea sp. nov., isolated from forest soil, and emended description of the genus Frateuria

2011 ◽  
Vol 61 (2) ◽  
pp. 443-447 ◽  
Author(s):  
Jia-Yue Zhang ◽  
Xing-Yu Liu ◽  
Shuang-Jiang Liu
Keyword(s):  
Forest Soil ◽  
Novel Species ◽  
Sequence Similarity ◽  
Changbai Mountains ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids ◽  
Emended Description ◽  
Negative Growth

A bacterial strain, designated VA24T, was isolated from forest soil of the Changbai Mountains, Heilongjiang province, China. Cells of strain VA24T were Gram-reaction-negative, aerobic, non-spore-forming, long rods, 0.3–0.5×2.0–3.0 μm in size and were motile by means of a subpolar flagellum. Strain VA24T was oxidase-positive and catalase-negative. Growth occurred at 21–36 °C, pH 5–10 and in 0–2 % (w/v) NaCl but did not occur at 37 °C. The predominant respiratory quinone was Q-8, the major polar lipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine and the major cellular fatty acids were iso-C15 : 0 (14.9 %), iso-C17 : 1 ω9c (14.1 %), iso-C17 : 0 (10.8 %) and iso-C16 : 0 (10.3 %). The DNA G+C content was 67.4 mol% (T m). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain VA24T was closely related to Frateuria aurantia IFO 13300T with 96.9 % sequence similarity. DNA–DNA relatedness of strain VA24T to F. aurantia DSM 6220T was 15.8 %. Based on its phenotypic and genotypic features, strain VA24T represents a novel species of the genus Frateuria, for which the name Frateuria terrea sp. nov. is proposed. The type strain is VA24T (=CGMCC 1.7053T =NBRC 104236T).

scholarly journals Shewanella mangrovi sp. nov., an acetaldehyde-degrading bacterium isolated from mangrove sediment

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2630-2634 ◽  
Author(s):  
Yang Liu ◽  
Xie-Xie Shang ◽  
Zhi-Wei Yi ◽  
Li Gu ◽  
Run-Ying Zeng
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Degrading Bacterium ◽  
Degrading Bacteria ◽  
Major Respiratory Quinone

A taxonomic study was carried out on strain YQH10T, which was isolated from mangrove sediment collected from Zhangzhou, China during the screening of acetaldehyde-degrading bacteria. Cells of strain YQH10T were Gram-stain-negative rods and pale brown-pigmented. Growth was observed at salinities from 0 to 11 % and at temperatures from 4 to 42 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YQH10T is affiliated to the genus Shewanella, showing the highest similarity with Shewanella haliotis DW01T (95.7 %) and other species of the genus Shewanella (91.4–95.6 %). The principal fatty acids were iso-C15 : 0 and C17 : 1ω8c. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA had a G+C content of 48.3 mol%. Strain YQH10T can completely degrade 0.02 % (w/v) acetaldehyde on 2216E at 28 °C within 48 h. Based on these phenotypic and genotypic data, strain YQH10T represents a novel species of the genus Shewanella, for which the name Shewanella mangrovi sp. nov. is proposed. The type strain is YQH10T ( = MCCC 1A00830T = JCM 30121T).

scholarly journals Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake

2011 ◽  
Vol 61 (7) ◽  
pp. 1506-1510 ◽  
Author(s):  
Yochan Joung ◽  
Kiseong Joh
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Artificial Lake ◽  
Respiratory Quinone ◽  
Major Respiratory Quinone ◽  
Type Strains

A non-motile, pale-yellow bacterium, designated strain HMD1056T, was isolated from an artificial lake located within the campus of Hankuk University of Foreign Studies, Yongin, Korea. The major fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 49.1 %) and iso-C15 : 0 (22.4 %). The major respiratory quinone was MK-7. The DNA G+C content was 46.9 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD1056T formed a lineage within the genus Mucilaginibacter and was closely related to the type strains of Mucilaginibacter ximonensis (95.4 % sequence similarity), Mucilaginibacter kameinonensis (94.5 %) and Mucilaginibacter paludis (93.4 %). On the basis of the evidence presented in this study, strain HMD1056T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter myungsuensis sp. nov. is proposed. The type strain is HMD1056T ( = KCTC 22746T  = CECT 7550T).

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