scholarly journals Micromonospora humi sp. nov., isolated from peat swamp forest soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1176-1181 ◽  
Author(s):  
Apakorn Songsumanus ◽  
Somboon Tanasupawat ◽  
Chitti Thawai ◽  
Khanit Suwanborirux ◽  
Takuji Kudo
Keyword(s):  
Forest Soil ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Phenotypic Differences ◽  
Peat Swamp ◽  
Close Relationship ◽  
Taxonomic Approach

A novel actinomycete, strain P0402T, was isolated from peat swamp forest soil collected in Thailand. Its taxonomic position was determined by using a polyphasic taxonomic approach. The chemotaxonomic characteristics of this strain matched those of the genus Micromonospora, i.e. the presence of meso-diaminopimelic acid and N-glycolyl muramic acid in the peptidoglycan, whole-cell sugar pattern D, phospholipid type II, and cellular fatty acid type 3b. Phylogenetic analysis based on 16S rRNA gene sequences revealed a close relationship between strain P0402T and Micromonospora coxensis JCM 13248T (99.0 % similarity), Micromonospora eburnea JCM 12345T (99.0 %), Micromonospora marina JCM 12870T (98.9 %), Micromonospora halophytica JCM 3125T (98.7 %), Micromonospora chalcea JCM 3031T (98.7 %), Micromonospora purpureochromogenes JCM 3156T (98.6 %) and Micromonospora aurantiaca JCM 10878T (98.5 %). It could be clearly distinguished from these type strains based on low levels of DNA–DNA relatedness and phenotypic differences. On the basis of the data presented, strain P0402T is suggested to represent a novel species of the genus Micromonospora, for which the name Micromonospora humi sp. nov. is proposed. The type strain is P0402T ( = JCM 15292T  = PCU 315T  = TISTR 1883T).

Acrocarpospora phusangensis sp. nov., isolated from a temperate peat swamp forest soil

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2174-2179 ◽  
Author(s):  
Nantawan Niemhom ◽  
Chanwit Suriyachadkun ◽  
Tomohiko Tamura ◽  
Chitti Thawai
Keyword(s):  
Forest Soil ◽  
Type Species ◽  
Biochemical Properties ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinomycete, strain PS33-18T, that formed club-shaped and spherical structures borne on the tip of the aerial mycelia was isolated from a temperate peat swamp forest soil in Phu-Sang National Park, Phayao Province, Thailand. The isolate contained glutamic acid, alanine and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS33-18T were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and ninhydrin-positive phosphoglycolipids. The predominant menaquinone was MK-9(H4). The major cellular fatty acids were C17 : 1ω8c, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA of strain PS33-18T was 71.0 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS33-18T should be classified in the genus Acrocarpospora . The level of similarity between this strain and the closely related species Acrocarpospora macrocephala NBRC 16266T was 98.3 %, Acrocarpospora pleiomorpha NBRC 16267T was 97.9 %, Acrocarpospora corrugata NBRC 13972T was 97.6 %, Herbidospora sakaeratensis NBRC 102641T was 97.6 % and Planotetraspora kaengkrachanensis NBRC 104272T was 97.3 %. DNA–DNA hybridization results and physiological and biochemical properties indicated that strain PS33-18T could be distinguished readily from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Acrocarpospora phusangensis sp. nov. is proposed. The type strain is PS33-18T ( = BCC 46906T = NBRC 108782T).

scholarly journals Actinomadura rayongensis sp. nov., isolated from peat swamp forest soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 890-895 ◽  
Author(s):  
Wongsakorn Phongsopitanun ◽  
Somboon Tanasupawat ◽  
Khanit Suwanborirux ◽  
Moriya Ohkuma ◽  
Takuji Kudo
Keyword(s):  
Forest Soil ◽  
Type Strain ◽  
Type Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinomycete strain RY35-68T, isolated from a peat swamp forest soil sample in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus Actinomadura based on morphological and chemotaxonomic characteristics. Cell-wall analysis revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan layer. The diagnostic sugar in whole-cell hydrolysates was identified as madurose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major cellular fatty acids were C16 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The genomic DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY35-68T was closely related to the species Actinomadura atramentaria JCM 6250T (97.5 %). The value of DNA–DNA relatedness between strain RY35-68T and A. atramentaria JCM 6250T was 37.6–42.6 %. On the basis of its phenotypic characteristics and these results mentioned, this strain could be distinguished from the closely related type strain and represents a novel species of the genus Actinomadura , for which the name Actinomadura rayongensis sp. nov. (type strain RY35-68T = JCM 19830T = TISTR 2211T = PCU 332T) is proposed.

scholarly journals Micromonospora marina sp. nov., isolated from sea sand

2010 ◽  
Vol 60 (3) ◽  
pp. 648-652 ◽  
Author(s):  
Somboon Tanasupawat ◽  
Suchada Jongrungruangchok ◽  
Takuji Kudo
Keyword(s):  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Phenotypic Differences ◽  
Sea Sand ◽  
Acid Type ◽  
Close Relationship ◽  
Micromonospora Chalcea ◽  
Type Strains ◽  
Fatty Acid Type

Two actinomycete strains, JSM1-1T and JSM1-3, were isolated from sea sand collected in Thailand. Their taxonomic position was determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora, i.e. the presence of meso-diaminopimelic acid and N-glycolyl muramic acid in the peptidoglycan, whole cell sugar pattern D, phospholipids type II, and cellular fatty acid type 3b. Phylogenetic analysis of 16S rRNA gene sequences revealed a close relationship between strains JSM1-1T and JSM1-3 (99.8 %), and between JSM1-1T and Micromonospora aurantiaca JCM 10878T (99.3 %), Micromonospora chalcea JCM 3031T (99.0 %), and Micromonospora coxensis JCM 13248 T (99.0 %). However, strains JSM1-1T and JSM1-3 could be clearly distinguished from these type strains by a low DNA–DNA relatedness and by phenotypic differences. On the basis of the data presented, a new species, Micromonospora marina sp. nov., is proposed. The type strain is JSM1-1T (=JCM 12870T =PCU 269T =TISTR 1566T).

Asanoa siamensis sp. nov., isolated from soil from a temperate peat swamp forest

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 66-71 ◽  
Author(s):  
Nantawan Niemhom ◽  
Chanwit Suriyachadkun ◽  
Tomohiko Tamura ◽  
Chitti Thawai
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Biochemical Properties ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinomycete strain, PS7-2T, which produced spore chains borne on the tips of short sporophores, was isolated from soil collected from a temperate peat swamp forest in Phu-Sang National Park, Phayao province, Thailand. The isolate contained glutamic acid, glycine, alanine, 3-hydroxy-diaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars were glucose, mannose, rhamnose and xylose, and the major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The predominant menaquinones were MK-10(H8) and MK-9(H8) and the predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.3 mol%. On the basis of 16S rRNA gene sequence analysis, strain PS7-2T clustered with members of the genus Asanoa and appeared most closely related to the type strains of Asanoa hainanensis (99.5 % sequence similarity), Asanoa iriomotensis (99.0 %), Asanoa ishikariensis (98.9 %) and Asanoa ferruginea (98.5 %). DNA–DNA hybridizations and some physiological and biochemical properties indicated that strain PS7-2T could be readily distinguished from its closest phylogenetic relatives. Based on the phenotypic and genotypic evidence and DNA–DNA relatedness values, strain PS7-2T represents a novel species in the genus Asanoa , for which the name Asanoa siamensis sp. nov. is proposed; the type strain is PS7-2T ( = BCC 41921T = NBRC 107932T).

scholarly journals Pseudonocardia acidicola sp. nov., a novel actinomycete isolated from peat swamp forest soil

2020 ◽  
Vol 70 (11) ◽  
pp. 5648-5653 ◽  
Author(s):  
Chollachai Klaysubun ◽  
Kenika Lipun ◽  
Kannika Duangmal
Keyword(s):  
Type Species ◽  
Peat Soil ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Polar Lipid Profile ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinobacterium, designated strain K10HN5T, was isolated from a peat soil sample collected from Kantulee peat swamp forest, Surat Thani Province, Thailand and its taxonomic position was determined using a polyphasic approach. Strain K10HN5T contained meso-diaminopimelic acid, arabinose, galactose, glucose and ribose in its whole-cell hydrolysates. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C16 : 0, iso-C15 : 0 and iso-C16 : 1H. Mycolic acids were not present. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine and phosphatidylinositol. The 16S rRNA gene sequence analysis indicated that it was closely related to Pseudonocardia bannensis DSM 45300T (97.9 %) and Pseudonocardia xinjiangensis JCM 11839T (97.9 %). Strain K10HN5T exhibited low average nucleotide identity and digital DNA–DNA hybridization values with P. bannensis DSM 45300T (82.6, 28.7 %) and P. xinjiangensis JCM11839T (76.3, 22.2 %). The DNA G+C content of strain K10HN5T was 72.4 mol%. Based on polyphasic data, strain K10HN5T represents a novel species of the genus Pseudonocardia , for which the name Pseudonocardia acidicola sp. nov. is proposed. The type strain is K10HN5T (=TBRC 10048T=NBRC 113897T).

scholarly journals Amycolatopsis acidicola sp. nov., isolated from peat swamp forest soil

2020 ◽  
Vol 70 (3) ◽  
pp. 1547-1554 ◽  
Author(s):  
Wee Fei Aaron Teo ◽  
Nantana Srisuk ◽  
Kannika Duangmal
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinobacterial strain, designated K81G1T, was isolated from a soil sample collected in Kantulee peat swamp forest, Surat Thani Province, Thailand, and its taxonomic position was determined using a polyphasic approach. Optimal growth of strain K81G1T occurred at 28–30 °C, at pH 5.0–6.0 and without NaCl. Strain K81G1T had cell-wall chemotype IV (meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and galactose as diagnostic sugars) and phospholipid pattern type II, characteristic of the genus Amycolatopsis . It contained MK-9(H4) as the predominant menaquinone, iso-C16 : 0, C17 : 0 cyclo and C16 : 0 as the major cellular fatty acids, and phospholipids consisting of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids. Based on 16S rRNA gene sequence similarity and phylogenetic analyses, strain K81G1T was most closely related to Amycolatopsis rhizosphaerae TBRC 6029T (97.8 % similarity), Amycolatopsis acidiphila JCM 30562T (97.8 %) and Amycolatopsis bartoniae DSM 45807T (97.6 %). Strain K81G1T exhibited low average nucleotide identity and digital DNA–DNA hybridization values with A. rhizosphaerae TBRC 6029T (76.4 %, 23.0 %), A. acidiphila JCM 30562T (77.9 %, 24.6 %) and A. bartoniae DSM 45807T (77.8 %, 24.3 %). The DNA G+C content of strain K81G1T was 69.7 mol%. Based on data from this polyphasic study, strain K81G1T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis acidicola sp. nov. is proposed. The type strain is K81G1T (=TBRC 10047T=NBRC 113896T).

Amycolatopsis acididurans sp. nov., isolated from peat swamp forest soil in Thailand

2020 ◽  
Author(s):  
Wee Fei Aaron Teo ◽  
Kenika Lipun ◽  
Nantana Srisuk ◽  
Kannika Duangmal
Keyword(s):  
Forest Soil ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Peat Swamp

scholarly journals Teunia siamensis f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area

2020 ◽  
Vol 70 (6) ◽  
pp. 3673-3678 ◽  
Author(s):  
Pannida Khunnamwong ◽  
Noppon Lertwattanasakul ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Peat Swamp ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

The strain DMKU-XD44, representing an anamorphic novel yeast species, was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Teunia cuniculi CBS 10309T was the most closely related species. The novel species differed from the T. cuniculi type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis, based on the combined sequences of the ITS region and the D1/D2 region, indicated that DMKU-XD44 represents a member of the Teunia clade in the Cryptococcaceae (Tremellales, Tremellomycetes, Agaricomycotina and Basidiomycota) and is phylogenetically distinct from other species of the genus Teunia in the clade. Therefore, DMKU-XD44 represents a novel species of the genus Teunia. The name Teunia siamensis f.a., sp. nov. is proposed. The holotype is DMKU-XD44, while the MycoBank number is MB 832816.

scholarly journals Identification and Characterization of Lipolytic Enzymes from a Peat-Swamp Forest Soil Metagenome

2010 ◽  
Vol 74 (9) ◽  
pp. 1848-1854 ◽  
Author(s):  
Benjarat BUNTERNGSOOK ◽  
Pattanop KANOKRATANA ◽  
Taksawan THONGARAM ◽  
Sutipa TANAPONGPIPAT ◽  
Tanaporn UENGWETWANIT ◽  
...  
Keyword(s):  
Forest Soil ◽  
Swamp Forest ◽  
Lipolytic Enzymes ◽  
Peat Swamp Forest ◽  
Peat Swamp ◽  
Soil Metagenome ◽  
Identification And Characterization

scholarly journals Dactylosporangium sucinum sp. nov., isolated from Thai peat swamp forest soil

2015 ◽  
Vol 68 (6) ◽  
pp. 379-384 ◽  
Author(s):  
Wongsakorn Phongsopitanun ◽  
Takuji Kudo ◽  
Moriya Ohkuma ◽  
Khanit Suwanborirux ◽  
Somboon Tanasupawat
Keyword(s):  
Forest Soil ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Peat Swamp
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