A novel actinomycete, strain PS33-18T, that formed club-shaped and spherical structures borne on the tip of the aerial mycelia was isolated from a temperate peat swamp forest soil in Phu-Sang National Park, Phayao Province, Thailand. The isolate contained glutamic acid, alanine and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS33-18T were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and ninhydrin-positive phosphoglycolipids. The predominant menaquinone was MK-9(H4). The major cellular fatty acids were C17 : 1ω8c, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA of strain PS33-18T was 71.0 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS33-18T should be classified in the genus
Acrocarpospora
. The level of similarity between this strain and the closely related species
Acrocarpospora macrocephala
NBRC 16266T was 98.3 %,
Acrocarpospora pleiomorpha
NBRC 16267T was 97.9 %,
Acrocarpospora corrugata
NBRC 13972T was 97.6 %,
Herbidospora sakaeratensis
NBRC 102641T was 97.6 % and
Planotetraspora kaengkrachanensis
NBRC 104272T was 97.3 %. DNA–DNA hybridization results and physiological and biochemical properties indicated that strain PS33-18T could be distinguished readily from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Acrocarpospora phusangensis sp. nov. is proposed. The type strain is PS33-18T ( = BCC 46906T = NBRC 108782T).
Identification and Characterization of Lipolytic Enzymes from a Peat-Swamp Forest Soil Metagenome