scholarly journals Deinococcus reticulitermitis sp. nov., isolated from a termite gut

2012 ◽  
Vol 62 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Wen Chen ◽  
Baojun Wang ◽  
Huazhu Hong ◽  
Hong Yang ◽  
Shuang-Jiang Liu
Keyword(s):  
Type Strain ◽  
Uv Light ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Polar Lipid Profile ◽  
Cellular Fatty Acids ◽  
Optimum Ph ◽  
Sequence Similarities

Bacterial strain TM-1T was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Cells of strain TM-1T were Gram-negative, spherical (1.0–2.0 µm in diameter), non-motile, non-sporulating and red-pigmented. Strain TM-1T was resistant to UV radiation, showing 34 % survival after exposure to UV light at a dose of 100 J m−2. Growth occurred at 20–40 °C (optimum, 30 °C), at pH 6.0–10.0 (optimum, pH 6.0–7.0), and in the presence of 0–1 % (w/v) NaCl (optimum, 0–0.4 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TM-1T was related to members of the genus Deinococcus, with sequence similarities ranging from 87.0 to 94.0 %. The peptidoglycan of strain TM-1T contained ornithine, alanine, glycine and glutamic acid. The most abundant cellular fatty acids of strain TM-1T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 22.3 %) and C16 : 0 (37.5 %). MK-8 was the predominant quinone. The polar lipid profile contained three glycophospholipids, six glycolipids, one aminolipid and three unknown lipids. DNA of the type strain had a G+C content of 65.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain TM-1T represents a novel species of the genus Deinococcus, for which the name Deinococcus reticulitermitis sp. nov. is proposed, with TM-1T ( = CGMCC 1.10218T = NBRC 106334T) as the type strain.

Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite

2012 ◽  
Vol 62 (2) ◽  
pp. 397-402 ◽  
Author(s):  
Rosa Margesin ◽  
Peter Schumann ◽  
De-Chao Zhang ◽  
Mersiha Redzic ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Polar Lipid Profile ◽  
Alpine Glacier ◽  
Cellular Fatty Acids

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08T, was isolated from alpine glacier cryoconite. Growth of strain Cr6-08T occurred at 1–25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08T is most closely related to members of the genus Arthrobacter. Strain Cr6-08T possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys–l-Ala4), MK-9(H2) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C15 : 0 and iso-C15 : 0) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08T was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain Cr6-08T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08T ( = DSM 23324T  = LMG 26052T  = CGMCC 1.10698T).

scholarly journals Aquabacterium limnoticum sp. nov., isolated from a freshwater spring

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 698-704 ◽  
Author(s):  
Wen-Ming Chen ◽  
Nian-Tsz Cho ◽  
Shwu-Harn Yang ◽  
A. B. Arun ◽  
Chiu-Chung Young ◽  
...  
Keyword(s):  
Hydroxy Fatty Acid ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Polar Lipid Profile ◽  
Optimum Ph ◽  
Freshwater Spring

A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4T, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 °C (optimum, 30–37 °C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4T, together with Aquabacterium fontiphilum CS-6T (96.4 % sequence similarity), Aquabacterium commune B8T (96.1 %), Aquabacterium citratiphilum B4T (95.5 %) and Aquabacterium parvum B6T (94.7 %), formed a deep line within the order Burkholderiales. Strain ABP-4T contained summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), C18 : 1 ω7c and C16 : 0 as predominant fatty acids. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA–DNA relatedness of strain ABP-4T with respect to recognized species of the genus Aquabacterium was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4T represents a novel species in the genus Aquabacterium, for which the name Aquabacterium limnoticum sp. nov. is proposed. The type strain is ABP-4T ( = BCRC 80167T = KCTC 23306T).

scholarly journals Brevundimonas basaltis sp. nov., isolated from black sand

2010 ◽  
Vol 60 (7) ◽  
pp. 1488-1492 ◽  
Author(s):  
Jung-Hye Choi ◽  
Min-Soo Kim ◽  
Seong Woon Roh ◽  
Jin-Woo Bae
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Black Sand ◽  
Dna Relatedness ◽  
Cellular Fatty Acids ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Type Strains ◽  
Sequence Similarities

A Gram-negative, aerobic, rod-shaped, motile Brevundimonas-like bacterial strain, J22T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Growth of strain J22T was observed in R2A medium at temperatures between 10 and 42 °C (optimum 30 °C), between pH 6.5 and 10.5 (optimum pH 7.5) and at a NaCl concentration between 0 and 4 % (w/v) (optimum 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J22T belonged to the genus Brevundimonas, with high sequence similarities of >97 % to the sequence of the type strains Brevundimonas alba CB88T, Brevundimonas lenta DS-18T, Brevundimonas variabilis CB17T, Mycoplana bullata TK0051T, Brevundimonas kwangchunensis KSL-102T, Brevundimonas intermedia CB63T, Brevundimonas subvibrioides CB81T and Brevundimonas bacteroides CB7T. Strain J22T exhibited DNA–DNA relatedness values of less than 22.2 % with the phylogenetically related species of the genus Brevundimonas. The DNA G+C content of strain J22T was 66.3 mol%. The predominant cellular fatty acids were C18 : 1 ω7c, C16 : 0 and C16 : 1 ω9c; C12 : 0 3-OH was present, which chemotaxonomically characterizes the members of the genus Brevundimonas. Phylogenetic, genomic and biochemical characteristics served to differentiate this isolate from recognized members of the genus Brevundimonas. Strain J22T (=KCTC 22177T=JCM 15911T) should be classified as a novel species in the genus Brevundimonas, for which the name Brevundimonas basaltis sp. nov. is proposed.

scholarly journals Gynuella sunshinyii gen. nov., sp. nov., an antifungal rhizobacterium isolated from a halophyte, Carex scabrifolia Steud

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 1038-1043 ◽  
Author(s):  
Eu Jin Chung ◽  
Jeong Ae Park ◽  
Che Ok Jeon ◽  
Young Ryun Chung
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Sequence Similarities

An antifungal bacterial strain, designated YC6258T, was isolated from the rhizosphere of a halophyte (Carex scabrifolia Steud.) growing in a tidal flat area of Namhae Island, Korea. Cells of the strain were Gram-stain-negative, facultatively anaerobic, moderately halophilic, rod-shaped and motile by a single polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC6258T formed a phyletic lineage distinct from members of the most closely related genera, Saccharospirillum and Reinekea, with less than 91.2 % sequence similarities. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and Summed feature 3 (C16 : 1ω7c/ C16 : 1ω6c). The quinone system of strain YC6258T consisted mainly of ubiquinone Q-8. The polar lipid profile exhibited phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unknown lipids. The DNA G+C content was 48.9 mol%. Based on the phylogenetic and phenotypic characteristics, strain YC6258T should be classified as a representative of a novel species in a novel genus for which the name Gynuella sunshinyii gen. nov., sp. nov. is proposed. The type strain is YC6258T (KCCM 43015T = NBRC 109345T).

scholarly journals Flavobacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond

2011 ◽  
Vol 61 (6) ◽  
pp. 1402-1407 ◽  
Author(s):  
Shih-Yi Sheu ◽  
Tzu-Fang Chiu ◽  
Chiu-Chung Young ◽  
A. B. Arun ◽  
Wen-Ming Chen
Keyword(s):  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Freshwater Shrimp ◽  
Phenotypic Data ◽  
Polar Lipid Profile ◽  
Shrimp Culture ◽  
Optimum Ph ◽  
Taxonomic Approach ◽  
Sequence Similarities

A bacterial strain, designated an-8T, was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain an-8T were Gram-reaction-negative, aerobic, rod-shaped and non-motile, formed yellow-pigmented colonies and grew at 15–30 °C (optimum 25 °C), pH 7–8 (optimum pH 8.0) and in 0–1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain an-8T belonged to the genus Flavobacterium and its most closely related neighbours were Flavobacterium terrigena DS-20T and Flavobacterium terrae R2A1-13T with sequence similarities of 95.1 and 94.9 %, respectively. Strain an-8T contained iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C16 : 0 3-OH, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and iso-C15 : 1 as the major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylserine and several unidentified polar lipids. The G+C content of the genomic DNA was 39.8 mol%. On the basis of the phylogenetic and phenotypic data, strain an-8T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium macrobrachii sp. nov. is proposed. The type strain is an-8T ( = BCRC 17965T  = LMG 25203T).

scholarly journals Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease

2010 ◽  
Vol 60 (10) ◽  
pp. 2371-2376 ◽  
Author(s):  
Kwang Kyu Kim ◽  
Keun Chul Lee ◽  
Heekyung Yu ◽  
Sungweon Ryoo ◽  
Youngkil Park ◽  
...  
Keyword(s):  
Pulmonary Disease ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Polar Lipid Profile ◽  
Diamino Acid

Strain KIT 00200-70066-1T was isolated from the sputum of a patient with pulmonary disease. Cells of the strain were Gram-variable, facultatively anaerobic, motile, spore-forming rods and formed colourless to white colonies on tryptic soy agar at 30 °C and pH 7. The pathogenicity of the strain is not known. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 as the major fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids in the polar lipid profile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Paenibacillus, sharing the highest levels of sequence similarity with Paenibacillus nanensis MX2-3T, Paenibacillus agaridevorans DSM 1355T and Paenibacillus alkaliterrae KSL-134T (95.4, 95.2 and 94.8 %, respectively), and that it occupied a distinct position within this genus. Combined phylogenetic and phenotypic data supported the conclusion that strain KIT 00200-70066-1T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus sputi sp. nov. is proposed; the type strain is KIT 00200-70066-1T (=KCTC 13252T =DSM 22699T).

scholarly journals Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost

2011 ◽  
Vol 61 (7) ◽  
pp. 1595-1600 ◽  
Author(s):  
Joong-Jae Kim ◽  
Hyun Mi Jin ◽  
Hyo Jung Lee ◽  
Che Ok Jeon ◽  
Eiko Kanaya ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Optimum Ph ◽  
Motile By Gliding

A strictly aerobic, Gram-stain-negative, yellow-pigmented, non-spore-forming, motile (by gliding), rod-shaped bacterium, designated strain 15F3T, was isolated from leaf-and-branch compost. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15F3T was most closely related to Flavobacterium reichenbachii WB 3.2-61T and formed a distinct phyletic lineage within the genus Flavobacterium, the type genus of the family Flavobacteriaceae. Growth was observed at 10–34 °C (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). No growth occurred in the presence of ≥2 % (w/v) NaCl. Strain 15F3T reduced nitrate to nitrogen and showed catalase activity but no oxidase activity. The predominant cellular fatty acids were iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major isoprenoid quinone was menaquinone-6. The G+C content of the genomic DNA was 31.1 mol%. On the basis of data from this polyphasic study, strain 15F3T may be classified as a representative of a novel species within the genus Flavobacterium, for which the name Flavobacterium banpakuense sp. nov. is proposed; the type strain is 15F3T ( = KACC 14225T  = JCM 16466T).

scholarly journals Lactobacillus vespulae sp. nov., isolated from gut of a queen wasp (Vespula vulgaris)

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3326-3332 ◽  
Author(s):  
Van-An Hoang ◽  
Yeon-Ju Kim ◽  
Ngoc-Lan Nguyen ◽  
Si-Kwan Kim ◽  
Deok-Chun Yang
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Housekeeping Genes ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Vespula Vulgaris ◽  
Optimum Ph ◽  
Peptidoglycan Structure ◽  
Sequence Similarities

A Gram-stain-positive, oxidase- and catalase-negative, rod-shaped, facultatively anaerobic bacterial strain, DCY75T, was isolated from a queen wasp (Vespula vulgaris). Growth occurred at 4–37 °C (optimum, 30 °C), at pH 3.5–8.0 (optimum, pH 5.0–6.0) and with ≤ 7.0 % (w/v) NaCl. Strain DCY75T produced gas during growth on glucose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DCY75T belonged to the genus Lactobacillus and was closely related to Lactobacillus sanfranciscensis ATCC 27651T and Lactobacillus lindneri DSM 20690T at sequence similarities of 96.7 and 96.4 %, respectively. A comparison of two housekeeping genes, pheS and rpoA, revealed that strain DCT75T was well separated from other species of the genus Lactobacillus. Strain DCY75T produced d- and l-lactic acid isomers in a ratio of 22.5 : 77.5 (v/v). The major fatty acids were summed feature 8 (comprising C18 :  1ω7c and/or C18 : 1ω6c), C16 : 0, C18 : 1ω9c and C18 : 0.The peptidoglycan structure was of the A4α (l-Lys–d-Asp) type. Cell-wall sugars were glucose, galactose and ribose. The DNA G+C content was 35.5 ± 1.3 mol%. Based on phenotypic and genotypic properties, strain DCY75T represents a novel species of the genus Lactobacillus, for which the name Lactobacillus vespulae sp. nov. is proposed. The type strain is DCY75T ( = KCTC 21023T = JCM 19742T).

scholarly journals Sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium

2007 ◽  
Vol 57 (8) ◽  
pp. 1868-1871 ◽  
Author(s):  
Sun-Young An ◽  
Tomomi Haga ◽  
Hiroaki Kasai ◽  
Keiichi Goto ◽  
Akira Yokota
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Brackish Lake ◽  
Cellular Fatty Acids ◽  
Type Strains ◽  
Sequence Similarities

Two Gram-positive, endospore-forming, rod-shaped bacterial strains, HG645T and HG711, were respectively isolated from surface water of a brackish lake and sediment of a fishery harbour in Japan and were subsequently characterized taxonomically using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains HG645T and HG711 are affiliated phylogenetically to the genus Sporosarcina, and they exhibit sequence similarities of 95.7–97.3 % to the type strains of Sporosarcina species. DNA–DNA relatedness between strain HG645T and the type strain of the phylogenetically related species Sporosarcina aquimarina was less than 10 %. The DNA G+C content of strains HG645T and HG711 were respectively 46.0 and 45.2 mol%. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell-wall peptidoglycan type (Lys–Glu), major cellular fatty acids (iso-C15 : 0 and anteiso-C15 : 0) and quinone type (MK-7) of the isolates support their affiliation to the genus Sporosarcina. On the basis of phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent a novel species of the genus Sporosarcina, for which the name Sporosarcina saromensis sp. nov. is proposed. The type strain is strain HG645T (=MBIC08270T=IAM 15429T =KCTC 13119T).

Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe)

2012 ◽  
Vol 62 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Byung-Chun Kim ◽  
Doo-Sang Park ◽  
Hyangmi Kim ◽  
Hyun-Woo Oh ◽  
Kang Hyun Lee ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Culturable Bacteria ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Diamino Acid ◽  
Isoprenoid Quinones

A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62T, was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62T grew at 15–30 °C and pH 5.0–8.5. The isoprenoid quinones were menaquinones MK-11 (77.1 %), MK-10 (11.7 %) and MK-12 (11.2 %). The major cellular fatty acids were anteiso-C15 : 0 (34.6 %), anteiso-C17 : 0 (29.8 %), iso-C16 : 0 (17.0 %) and cyclohexyl-C17 : 0 (11.4 %). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62T was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62T was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11T (98.08 % similarity). The level of DNA–DNA relatedness between strain RB-62T and H. ginsengi wged11T was 43.2 % (reciprocal 66.7 %). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62T from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62T is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62T ( = KCTC 19653T = JCM 16117T).

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