scholarly journals Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. scarabaeorum and T. gamsii

2004 ◽  
Vol 54 (3) ◽  
pp. 975-986 ◽  
Author(s):  
Wouter J. Middelhoven ◽  
Gloria Scorzetti ◽  
Jack W. Fell
Keyword(s):  
Type Strain ◽  
Phylogenetic Trees ◽  
Novel Species ◽  
Large Subunit ◽  
Carbon Sources ◽  
Unknown Origin ◽  
Maximum Growth ◽  
Basidiomycetous Yeast ◽  
Internal Transcribed Spacer Region ◽  
Yeast Genus

Phylogenetic trees of the anamorphic basidiomycetous yeast genus Trichosporon Behrend, based on molecular sequence analysis of the internal transcribed spacer region and the D1/D2 region of the large subunit of ribosomal (26S) DNA, are presented. This study includes three novel species from soils, Trichosporon vadense sp. nov. (type strain, CBS 8901T), Trichosporon smithiae sp. nov. (type strain, CBS 8370T) and Trichosporon gamsii sp. nov. (type strain, CBS 8245T), one novel species from an insect, Trichosporon scarabaeorum sp. nov. (type strain, CBS 5601T) and one species of unknown origin, Trichosporon dehoogii sp. nov. (type strain, CBS 8686T). The phylogenetic positions and physiological characteristics that distinguish the new taxa from related species, based partly on growth tests that are not traditionally used in yeast taxonomy (uric acid, ethylamine, l-4-hydroxyproline, tyramine and l-phenylalanine as sources of carbon and nitrogen, and polygalacturonate, quinate, 4-ethylphenol, phloroglucinol, 2,3-dihydroxybenzoate and orcinol as sole carbon sources), are discussed. Assimilation of l-rhamnose and erythritol and maximum growth temperature were also used to delineate species.

scholarly journals Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals

2004 ◽  
Vol 54 (2) ◽  
pp. 623-627 ◽  
Author(s):  
Asuka Hirai ◽  
Rui Kano ◽  
Koichi Makimura ◽  
Eduardo Robson Duarte ◽  
Júnia Soares Hamdan ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Yeast Genus ◽  
26S Rdna ◽  
Lipid Source ◽  
Rdna Sequences

Five isolates of a novel species of the yeast genus Malassezia were isolated from animals in Japan and Brazil. Phylogenetic trees based on the D1/D2 domains of the large-subunit (26S) rDNA sequences and nucleotide sequences of the internal transcribed spacer 1 region showed that the isolates were conspecific and belonged to the genus Malassezia. They were related closely to Malassezia dermatis and Malassezia sympodialis, but were clearly distinct from these two species and the other six species of Malassezia that have been reported, indicating that they should be classified as a novel species, Malassezia nana sp. nov. Morphologically and physiologically, M. nana resembles M. dermatis and M. sympodialis, but can be distinguished from these species by its inability to use Cremophor EL (Sigma) as the sole lipid source and to hydrolyse aesculin. The type strain of M. nana is NUSV 1003T (=CBS 9557T=JCM 12085T).

Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 3115-3123 ◽  
Author(s):  
Neža Čadež ◽  
Dénes Dlauchy ◽  
Peter Raspor ◽  
Gábor Péter
Keyword(s):  
Sequence Analysis ◽  
Olive Oil ◽  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Virgin Olive Oil ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Lsu Rrna

Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1α (EF-1α) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322T = CBS 12778T = NRRL Y-63657T), Ogataea histrianica sp. nov. (type strain: ZIM 2463T = CBS 12779T = NRRL Y-63658T) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896T = CBS 12735T = NRRL Y-63656T).

scholarly journals Yamadazyma terventina sp. nov., a yeast species of the Yamadazyma clade from Italian olive oils

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 372-376 ◽  
Author(s):  
Gino Ciafardini ◽  
Biagi Angelo Zullo ◽  
Livio Antonielli ◽  
Laura Corte ◽  
Luca Roscini ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Internal Transcribed Spacer Region ◽  
Gene Encoding ◽  
Olive Oils ◽  
Yeast Strains ◽  
D2 Domain ◽  
5.8S Rrna

During an investigation of olive oil microbiota, three yeast strains were found to be divergent from currently classified yeast species according to the sequences of the D1/D2 domain of the gene encoding the rRNA large subunit (LSU) and the internal transcribed spacer region including the gene for 5.8S rRNA. Phylogenetic analysis revealed that these strains, designated CBS 12509, CBS 12510T and CBS 12511, represent a novel anascosporogenous species described herein as Yamadazyma terventina sp. nov; the type strain is DAPES 1924T ( = CBS 12510T = NCAIM Y.02028T). This novel species was placed in the Yamadazyma clade, with Yamadazyma scolyti, Candida conglobata and Candida aaseri as closest relatives. Y. terventina differs from the above-mentioned species in the ability to strongly assimilate dl-lactate and weakly assimilate ethanol.

scholarly journals Bullera cylindrica sp. nov., Bullera hubeiensis sp. nov. and Bullera nakasei sp. nov., ballistoconidium-forming yeast species from plant leaves

2004 ◽  
Vol 54 (5) ◽  
pp. 1877-1882 ◽  
Author(s):  
Qi-Ming Wang ◽  
Feng-Yan Bai ◽  
Hui-Zhong Lu ◽  
Jian-Hua Jia ◽  
Masako Takashima
Keyword(s):  
Type Strain ◽  
Phylogenetic Trees ◽  
Yeast Species ◽  
Novel Species ◽  
The Novel ◽  
Plant Leaves ◽  
Internal Transcribed Spacer Region ◽  
26S Rdna ◽  
D2 Domain ◽  
Taxonomic Characterization

Among yeasts isolated from plant leaves collected in different regions of China that form whitish or yellowish colonies and symmetrical ballistoconidia, four strains were shown to represent three novel Bullera species by conventional and molecular taxonomic characterization. The novel species are described as Bullera cylindrica sp. nov. (type strain CB 169T=AS 2.2308T=CBS 9744T), Bullera hubeiensis sp. nov. (type strain HX 19.3T=AS 2.2466T=CBS 9747T) and Bullera nakasei sp. nov. (type strain HX 15.5T=AS 2.2435T=CBS 9746T). These three species, and another eight previously described Bullera species represented by Bullera mrakii, formed a strongly supported distinct clade among the hymenomycetous yeasts in each of the phylogenetic trees drawn from the 26S rDNA D1/D2 domain and the internal transcribed spacer region sequences.

scholarly journals Characterization and Phylogeny of Fungi from Industrial Wastewater Using Highly Conserved Multiple Gene Loci

2021 ◽  
Author(s):  
Blessing Amaka Ezeonuegbu ◽  
Dauda Abdullahi Machido ◽  
Clement Z. Whong ◽  
Wisdom S. Japhet ◽  
Clement Ameh Yaro ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Industrial Effluent ◽  
Pcr Amplification ◽  
Morphological Characterization ◽  
Likelihood Method ◽  
Internal Transcribed Spacer Region ◽  
Fungal Isolates ◽  
Fungal Dna Extraction

Abstract The aim of this study was isolation and molecular characterization of fungi from untreated industrial effluent by multigene phylogenetic analyses. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubulin (Ben A), calmodulin (CaM), and DNA-directed RNA polymerase second large subunit (RPB2) genes, along with morphological characterization and species diversity. Fungal DNA extraction kits and primers sets for the selected genes were purchased and used following the manufacturer’s instructions. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. All the sequences from this study were deposited in GenBank and the accession number assigned. Phylogenetic trees of the fungal isolates were drawn for each gene by the Maximum Likelihood method using MEGA 7.0 software. Fifteen (15) Fungi species belonging to four genera of Aspergillus, Penicillium, Fusarium and Trichoderma with Aspergillus as the predominant genus were identified.

scholarly journals Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1855-1859 ◽  
Author(s):  
Ana Raquel O. Santos ◽  
Elisa S. Faria ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Mangifera Indica ◽  
Large Subunit ◽  
Its Region ◽  
Methylotrophic Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Mango Leaves

Five strains of a novel methanol-assimilating yeast species were isolated from mango (Mangifera indica) leaves collected at the campus of the Federal University of Minas Gerais in Brazil. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the Ogataea clade and is related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogensis and Candida xyloterini (Ogataea clade). The novel species differs in the D1/D2 domains of the large subunit of the rRNA gene by 12 to 40 substitutions from these Ogataea species. The name Ogataea mangiferae sp. nov. is proposed for this novel species. The type strain of Ogataea mangiferae sp. nov. is UFMG-CM-Y253T ( = CBS 13492T). The Mycobank number is MB 811646.

Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 393-397 ◽  
Author(s):  
Stephen A. James ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Kathryn Cross ◽  
Christopher J. Bond ◽  
...  
Keyword(s):  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Lsu Rrna Gene

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004T) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004T ( = CBS 12653T = NCYC 3782T) designated as the type strain.

scholarly journals Kocuria koreensis sp. nov., isolated from fermented seafood

2010 ◽  
Vol 60 (1) ◽  
pp. 140-143 ◽  
Author(s):  
Eun-Jin Park ◽  
Seong Woon Roh ◽  
Min-Soo Kim ◽  
Mi-Ja Jung ◽  
Kee-Sun Shin ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Genomic Dna ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Cellular Fatty Acids ◽  
The 16S Rrna Gene

A Gram-positive, aerobic, non-motile and coccoid actinobacterium, designated P31T, was isolated from a traditional, fermented seafood. The strain was catalase-positive and oxidase-negative. Cells grew in the presence of 0–15.0 % (w/v) NaCl, and at pH 5–10 and 15–37 °C. Major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. Strain P31T contained MK-7 as the predominant menaquinone. The DNA G+C content of the genomic DNA of strain P31T was 65.2 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain P31T was most closely related to Kocuria kristinae DSM 20032T, with 96.9 % similarity, and these two strains clustered together in constructed phylogenetic trees. The DNA–DNA hybridization value between strain P31T and K. kristinae DSM 20032T was 21.1 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it is suggested that strain P31T represents a novel species of the genus Kocuria, for which the name Kocuria koreensis sp. nov. is proposed. The type strain is P31T (=KCTC 19595T=JCM 15915T).

scholarly journals Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment

2004 ◽  
Vol 54 (2) ◽  
pp. 377-380 ◽  
Author(s):  
Hana Yi ◽  
Kyung Sook Bae ◽  
Jongsik Chun
Keyword(s):  
Tidal Flat ◽  
Type Strain ◽  
Novel Species ◽  
Carbon Sources ◽  
Halophilic Bacterium ◽  
Sister Group ◽  
Monophyletic Clade ◽  
Sequence Analyses ◽  
16S Rdna Sequence ◽  
Moderate Relationship

A Gram-negative, aerobic, halophilic bacterium, designated strain JC2041T, was isolated from a sediment sample of getbol, the Korean tidal flat. Results of 16S rDNA sequence analyses indicated a moderate relationship to Thalassomonas viridans within the γ-Proteobacteria (94·9 % similarity). Depending on the tree-making algorithm used, the isolate either formed a monophyletic clade with T. viridans or was recovered as a sister group of a class containing the genera Thalassomonas and Colwellia. Phenotypic features of the getbol isolate were similar to those of T. viridans, but several physiological and chemotaxonomic properties, including nitrate reduction, amylase, lecithinase, Tweenase and utilization of 13 carbon sources, distinguished strain JC2041T from T. viridans. The polyphasic data presented in this study indicate that the isolate should be classified as a novel species in the genus Thalassomonas. The name Thalassomonas ganghwensis sp. nov. is therefore proposed for the getbol isolate; the type strain is JC2041T (=IMSNU 14005T=KCTC 12041T=DSM 15355T).

scholarly journals Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments

2011 ◽  
Vol 61 (9) ◽  
pp. 2320-2327 ◽  
Author(s):  
Carolina H. Pohl ◽  
Martha S. Smit ◽  
Jacobus Albertyn
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Carbon Sources ◽  
The Other ◽  
26S Rdna ◽  
Rdna Sequencing ◽  
Physiological Tests

Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499T and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499T  = NRRL Y-48408T), Rhodotorula pini sp. nov. (type strain CBS 10735T  = NRRL Y-48410T), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598T  = NRRL Y-48407T) and Rhodotorula orientis sp. nov. (type strain CBS 8594T  = NRRL Y-48719T) are proposed. R. eucalyptica and R. pini can also utilize limonene.

Sign in / Sign up

Export Citation Format

Share Document