Marinobacter antarcticus sp. nov., a halotolerant bacterium isolated from Antarctic intertidal sandy sediment

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1838-1844 ◽  
Author(s):  
Chang Liu ◽  
Chun-Xiao Chen ◽  
Xi-Ying Zhang ◽  
Yong Yu ◽  
Ang Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Tween 80 ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30T, was isolated from Antarctic intertidal sandy sediment. The strain grew at 4–35 °C (optimum, 25 °C) and in 0–25 % (w/v) NaCl (optimum, 3.0–4.0 %). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C18 : 1ω9c, C16 : 1ω9c, C12 : 0 3-OH and C12 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30T was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30T was affiliated with the genus Marinobacter . It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus Marinobacter , namely Marinobacter maritimus (98.3 %), Marinobacter psychrophilus (98.1 %) and Marinobacter goseongensis (97.1 %), but the DNA–DNA relatedness values between strain ZS2-30T and the above three species were all lower than 45 %. Moreover, strain ZS2-30T could be distinguished from closely related species of the genus Marinobacter by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30T is considered to represent a novel species in the genus Marinobacter , for which the name Marinobacter antarcticus sp. nov. is proposed. The type strain of Marinobacter antarcticus is ZS2-30T ( = CGMCC 1.10835T = KCTC 23684T).

Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 685-690 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Chul-Hyung Kang ◽  
Yong-Taek Jung ◽  
So-Jung Kang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Oyster Farm

A Gram-staining-negative, non-spore-forming, aerobic, non-flagellated, non-gliding, rod-shaped bacterium, designated strain T-y7T, was isolated from seawater collected in an oyster farm in the South Sea, South Korea, and subjected to a polyphasic study. Strain T-y7T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, strain T-y7T fell within a clade comprising Bizionia species. It formed a coherent cluster with the type strains of Bizionia algoritergicola , B. argentinensis , B. echini and B. myxarmorum , with which it exhibited 16S rRNA gene sequence similarities of 97.4–98.9 % and mean DNA–DNA relatedness values of 11–27 %. Strain T-y7T had MK-6 as its predominant menaquinone and iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0 and iso-C17 : 0 3-OH as its major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The genomic DNA G+C content was 37.1 mol%. Its phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain T-y7T does not belong to any established Bizionia species. On the basis of the data presented, strain T-y7T is considered to represent a novel species of the genus Bizionia , for which the name Bizionia hallyeonensis sp. nov. is proposed. The type strain is T-y7T ( = KCTC 23881T  = CCUG 62110T).

scholarly journals Lutibacter oricola sp. nov., a marine bacterium isolated from seawater

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 485-490 ◽  
Author(s):  
Hye-Ri Sung ◽  
Kee-Sun Shin ◽  
Sa-Youl Ghim
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Seawater Samples

A bacterial strain, UDC377T, was isolated from seawater samples collected at Seo-do on the coast of Dokdo island in the East Sea, and was subjected to taxonomic study using a polyphasic approach. Strain UDC377T was pale-yellow, Gram-staining-negative, non-motile, rod-shaped and aerobic. The strain grew optimally at 25–28 °C, in the presence of 2 % (w/v) NaCl and at pH 7.0–7.5. Strain UDC377T produced carotenoid pigments; however, it did not produce flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC377T clustered with members of the genus Lutibacter and appeared most closely related to Lutibacter agarilyticus KYW566T (96.0 % 16S rRNA gene sequence similarity) followed by L. aestuarii MA-My1T (95.0 %), L. litoralis CL-TF09T (94.9 %), L. maritimus S7-2T (94.1 %) and L. flavus IMCC1507T (94.0 %). The DNA G+C content of strain UDC377T was 30.8 mol%. Strain UDC377T contained MK-6 as the predominant menaquinone, iso-C15 : 0 3-OH, iso-C15 : 0 and iso-C16 : 0 3-OH as the major fatty acids, and phosphatidylethanolamine, two unknown aminolipids and six unknown lipids as the major polar lipids. Based on phenotypic properties and phylogenetic data presented, strain UDC377T is considered to represent a novel species of the genus Lutibacter , for which the name Lutibacter oricola sp. nov. is proposed. The type strain is UDC377T ( = DSM 24956T = KCTC 23668T).

scholarly journals Amycolatopsis rhabdoformis sp. nov., an actinomycete isolated from a tropical forest soil

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1786-1793 ◽  
Author(s):  
Wallace Rafael Souza ◽  
Rafael Eduardo Silva ◽  
Michael Goodfellow ◽  
Kanungnid Busarakam ◽  
Fernanda Sales Figueiro ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Gene Tree ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type

Strain SB026T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847T, Amycolatopsis plumensis NRRL B-24324T, Amycolatopsis tolypomycina DSM 44544T and Amycolatopsis vancoresmycina NRRL B-24208T. It was related most closely to A. bullii NRRL B-24847T (99.0 % 16S rRNA gene sequence similarity), but was distinguished from this strain by a low level of DNA–DNA relatedness (~46 %) and discriminatory phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the isolate should be classified in the genus Amycolatopsis as representing a novel species, Amycolatopsis rhabdoformis sp. nov. The type strain is SB026T ( = CBMAI 1694T = CMAA 1285T = NCIMB 14900T).

scholarly journals Erythrobacter pelagi sp. nov., a member of the family Erythrobacteraceae isolated from the Red Sea

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1348-1353 ◽  
Author(s):  
Hui-xian Wu ◽  
Pok Yui Lai ◽  
On On Lee ◽  
Xiao-jian Zhou ◽  
Li Miao ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Red Sea ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Type Strains

A novel Gram-negative, aerobic, catalase- and oxidase-positive, non-sporulating, non-motile, rod-shaped bacterium, designated strain UST081027-248T, was isolated from seawater of the Red Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UST081027-248T fell within the genus Erythrobacter . Levels of 16S rRNA gene sequence similarity between the novel strain and the type strains of Erythrobacter species ranged from 95.3 % (with Erythrobacter gangjinensis ) to 98.2 % (with Erythrobacter citreus ). However, levels of DNA–DNA relatedness between strain UST081027-248T and the type strains of closely related species were below 70 %. Optimal growth of the isolate occurred in the presence of 2.0 % NaCl, at pH 8.0–9.0 and at 28–36 °C. The isolate did not produce bacteriochlorophyll a. The predominant cellular fatty acids were C17 : 1ω6c, summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C15 : 0 2-OH. The genomic DNA G+C content of strain UST081027-248T was 60.4 mol%. Phenotypic properties and phylogenetic distinctiveness clearly indicated that strain UST081027-248T represents a novel species of the genus Erythrobacter , for which the name Erythrobacter pelagi sp. nov. is proposed. The type strain is UST081027-248T ( = JCM 17468T = NRRL 59511T).

scholarly journals Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov.

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3768-3774 ◽  
Author(s):  
Naysim LO ◽  
Hyo Jung Kang ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Degrading Bacterium

A Gram-staining-negative, facultatively aerobic bacterium, designated SM-2T, was isolated from a sea-tidal flat of Yellow Sea, South Korea. Cells were catalase- and oxidase-positive motile rods with a single polar flagellum. Growth of strain SM-2T was observed at 10–37 °C (optimum, 25–30 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5) and in the presence of 0–11 % (w/v) NaCl (optimum, 2 %). Strain SM-2T contained ubiquinone-8 (Q-8) as the sole isoprenoid quinone and C17 : 1ω8c, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C17 : 0 and C18 : 1ω7c as the major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified lipid were identified as the major cellular polar lipids. The G+C content of the genomic DNA was 52.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SM-2T formed a tight phyletic lineage with Zhongshania antarctica ZS5-23T, Zhongshania guokunii ZS6-22T and Spongiibacter borealis CL-AS9T, but that S. borealis CL-AS9T was distinct from other species of the genus Spongiibacter . Based on 16S rRNA gene sequence similarities, strain SM-2T was most closely related to S. borealis CL-AS9T, Z. antarctica ZS5-23T and Z. guokunii ZS6-22T, with similarities of 99.5 %, 98.9 % and 98.7 %, respectively, but the DNA–DNA hybridization values among these species were clearly lower than 70 %. On the basis of chemotaxonomic data and molecular properties, we propose strain SM-2T represents a novel species of the genus Zhongshania with the name Zhongshania aliphaticivorans sp. nov. (type strain SM-2T = KACC 18120T = JCM 30138T). We also propose the transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov. (type strain CL-AS9T = KCCM 90094T = JCM 17304T).

Celeribacter baekdonensis sp. nov., isolated from seawater, and emended description of the genus Celeribacter Ivanova et al. 2010

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1359-1364 ◽  
Author(s):  
Soo-Young Lee ◽  
Sooyeon Park ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, non-motile, ovoid or rod-shaped bacterial strain, designated L-6T, was isolated from seawater of Baekdo harbour of the East Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain L-6T grew optimally at 30 °C, at pH 7.5–8.0 and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain L-6T formed a cluster with the type strain of Celeribacter neptunius at a bootstrap resampling value of 100 %. Strain L-6T exhibited 16S rRNA gene sequence similarity values of 97.7 % to C. neptunius H 14T and of less than 96.2 % to the type strains of other species used in the phylogenetic analysis. The G+C content of the chromosomal DNA of strain L-6T was 60.9 mol%. The predominant ubiquinone found in strain L-6T and C. neptunius CIP 109922T was ubiquinone-10 (Q-10). The predominant fatty acid of strain L-6T and C. neptunius CIP 109922T was C18 : 1ω7c. The major polar lipids of strain L-6T were phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The mean level of DNA–DNA relatedness between strain L-6T and C. neptunius CIP 109922T was 17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain L-6T is distinguishable from C. neptunius . On the basis of the data presented, strain L-6T is considered to represent a novel species of the genus Celeribacter , for which the name Celeribacter baekdonensis sp. nov. is proposed. The type strain is L-6T ( = KCTC 23497T  = CCUG 60799T).

scholarly journals Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 163-168 ◽  
Author(s):  
Rosanna A. Alegado ◽  
Jonathan D. Grabenstatter ◽  
Richard Zuzow ◽  
Andrea Morris ◽  
Sherri Y. Huang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Core Sample ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Unknown Phospholipid

A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1T, was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1T grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1T contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C15 : 0 (29.4 %), iso-C17 : 1ω9c (18.5 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 11.3 %). The major polar lipids detected in strain PR1T were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1T fell within the cluster comprising the genus Algoriphagus and was most closely related to Algoriphagus halophilus JC 2051T (95.4 % sequence similarity) and Algoriphagus lutimaris S1-3T (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1T and the type strains of other species of the genus Algoriphagus were in the range 91–95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1T demonstrated that this strain was distinct from other members of the genus Algoriphagus , including its closest relative, A. halophilus . Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1T should be placed in the genus Algoriphagus as a representative of a novel species, for which the name Algoriphagus machipongonensis sp. nov. is proposed. The type strain is PR1T ( = ATCC BAA-2233T  = DSM 24695T).

Dyella jiangningensis sp. nov., a γ-proteobacterium isolated from the surface of potassium-bearing rock

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3154-3157 ◽  
Author(s):  
Fei Zhao ◽  
Xin-qi Guo ◽  
Peng Wang ◽  
Lin-yan He ◽  
Zhi Huang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Many

A Gram-stain-negative, aerobic, motile with one polar flagellum γ-proteobacterium, designated strain SBZ3-12T, was isolated from surfaces of weathered potassic trachyte. Phylogenetic analysis of this strain based on 16S rRNA gene sequences showed that it was most closely related to Dyella japonica XD53T (97.9 % 16S rRNA gene sequence similarity), Dyella terrae JS14-6T (97.7 %), Dyella soli JS12-10T (97.5 %) and Dyella koreensis BB4T (97.0 %). The DNA G+C content of strain SBZ3-12T was 64.0 mol%. In addition, iso-C17 : 1ω9c, iso-C15 : 0 and iso-C16 : 0 were the major cellular fatty acids and ubiquinone Q-8 was the predominant respiratory quinone. The low DNA–DNA relatedness values between strain SBZ3-12T and recognized species of the genus Dyella and the many phenotypic properties supported the classification of strain SBZ3-12T as a representative of a novel species of the genus Dyella , for which the name Dyella jiangningensis sp. nov. is proposed. The type strain is SBZ3-12T ( = CCTCC AB 2012160T = KACC 16539T = DSM 26119T).

Reyranella soli sp. nov., isolated from forest soil, and emended description of the genus Reyranella Pagnier et al. 2011

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3164-3167 ◽  
Author(s):  
Soo-Jin Kim ◽  
Jae-Hyung Ahn ◽  
Tae-Hyung Lee ◽  
Hang-Yeon Weon ◽  
Seung-Beom Hong ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Forest Soil ◽  
Type Species ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated KIS14-15T, was isolated from forest soil of Baengnyeong Island in the Yellow Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain KIS14-15T grew optimally at 30 °C, at pH 6.0–7.0 and without NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain KIS14-15T formed a cluster with the strains of Reyranella massiliensis with a bootstrap resampling value of 100 %. Strain KIS14-15T exhibited 16S rRNA gene sequence similarity values of 98.0 % to R. massiliensis 521T and of less than 89 % to the type strains of other taxa. The mean level of DNA–DNA relatedness between strain KIS14-15T and R. massiliensis KACC 16548T was 21 % (reciprocal, 24 %). The predominant ubiquinone found in strain KIS14-15T and R. massiliensis KACC 16548T was ubiquinone-10 (Q-10). The predominant fatty acids of strain KIS14-15T and R. massiliensis KACC 16548T were C18 : 1ω7c, C18 : 1 2-OH and 11-methyl C18 : 1ω7c. Total polar lipids of strain KIS14-15T were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain KIS14-15T is distinguishable from R. massiliensis . On the basis of the data presented, strain KIS14-15T is considered to represent a novel species of the genus Reyranella , for which the name Reyranella soli sp. nov. is proposed. The type strain is KIS14-15T ( = KACC 13034T = NBRC 108950T).

scholarly journals Winogradskyella aquimaris sp. nov., isolated from seawater

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1814-1818 ◽  
Author(s):  
Soo-Young Lee ◽  
Sooyeon Park ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
South Sea ◽  
Winogradskyella Poriferorum

A Gram-negative, non-flagellated, motile by gliding, aerobic rod, designated DPG-24T, was isolated from seawater of Geoje Island in the South Sea, Korea. Strain DPG-24T grew optimally at 30–37 °C, at pH 7.0–7.5 and with 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DPG-24T belonged to the genus Winogradskyella and clustered with Winogradskyella poriferorum UST030701-295T and Winogradskyella exilis 022-2-26T. Strain DPG-24T exhibited 97.6 and 95.8 % 16S rRNA gene sequence similarities with W. poriferorum UST030701-295T and W. exilis 022-2-26T, respectively, and 92.4–95.7 % with other members of the genus Winogradskyella . Strain DPG-24T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The DNA G+C content was 36.7 mol%. DNA–DNA relatedness between the isolate and W. poriferorum JCM 12885T was 10.8 %. The differential phenotypic properties and phylogenetic and genetic distinctiveness enabled strain DPG-24T to be differentiated from recognized members of the genus Winogradskyella . On the basis of the data presented, strain DPG-24T is considered to represent a novel species of the genus Winogradskyella , for which the name Winogradskyella aquimaris sp. nov. is proposed; the type strain is DPG-24T ( = KCTC 23502T  = CCUG 60798T).

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