Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 254-259 ◽  
Author(s):  
Nathaniel A. Losey ◽  
Bradley S. Stevenson ◽  
Susanne Verbarg ◽  
Stephen Rudd ◽  
Edward R. B. Moore ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
New Family ◽  
Terrestrial Hot Spring

A novel bacterial strain designated HA-01T was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rod-shaped, aerobic, chemo-organotrophic, oxidase- and catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37–60 °C, with an optimum between 45 and 50 °C, and at pH 6.5–8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01T were Solimonas aquatica NAA-16T (93.8 %), Solimonas flava CW-KD 4T (94.1 %), Solimonas soli DCY12T (93.1 %), Solimonas variicoloris MN28T (94.0 %), Nevskia ramosa Soe1T (91.2 %) and Hydrocarboniphaga effusa AP103T (91.1 %). Major fatty acids consisted of C16 : 0, iso-C16 : 0, C16 : 1ω5c and summed feature 8 (C18 : 1ω9c, C18 : 1ω7c and C18 : 1ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01T represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01T ( = DSM 23609T = CCUG 59713T). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.

Caloramator quimbayensis sp. nov., an anaerobic, moderately thermophilic bacterium isolated from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1396-1402 ◽  
Author(s):  
Carolina Rubiano-Labrador ◽  
Sandra Baena ◽  
Carolina Díaz-Cárdenas ◽  
Bharat K. C. Patel
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Glucose Fermentation ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Terrestrial Hot Spring

An anaerobic, moderately thermophilic, terminal-spore-forming bacterium, designated strain USBA AT, was isolated from a terrestrial hot spring located at an altitude of 2683 m in the Andean region of Colombia (04° 50′ 14.0″ N 75° 32′ 53.4″ W). Cells of strain USBA AT were Gram-stain-positive, straight to slightly curved rods (0.9×2.5 µm), that were arranged singly or in pairs, and were motile by means of flagella. Growth occurred at 37–55 °C and pH 6.0–8.0, with a doubling time of 2 h under the optimal conditions (50 °C and pH 7.0). Glucose fermentation in strain USBA AT required yeast extract or peptone (each at 0.2 %, w/v). The novel strain fermented sugars, amino acids, Casamino acids, propanol, propionate, starch and dextrin, but no growth was observed on galactose, lactose, xylose, histidine, serine, threonine, benzoate, butyrate, lactate, pyruvate, succinate, methanol, ethanol, glycerol, casein, gelatin or xylan. The end products of glucose fermentation were formate, acetate, ethanol and lactate. Strain USBA AT did not grow autotrophically (with CO2 as carbon source and H2 as electron donor) and did not reduce thiosulfate, sulfate, elemental sulfur, sulfite, vanadium (V) or Fe (III) citrate. Growth of strain USBA AT was inhibited by ampicillin, chloramphenicol, kanamycin, penicillin and streptomycin (each at 10 µg ml−1). The predominant fatty acids were iso-C15 : 0, C16 : 0 and iso-C17 : 0 and the genomic DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis indicated that strain USBA AT belonged in the phylum Firmicutes and that its closest relative was Caloramator viterbiensis JW/MS-VS5T (95.0 % sequence similarity). A DNA–DNA relatedness value of only 30 % was recorded in hybridization experiments between strain USBA AT and Caloramator viterbiensis DSM 13723T. Based on the phenotypic, chemotaxonomic and phylogenetic evidence and the results of the DNA–DNA hybridization experiments, strain USBA AT represents a novel species of the genus Caloramator , for which the name Caloramator quimbayensis sp. nov. is proposed. The type strain is USBA AT ( = CMPUJ U833T  = DSM 22093T).

scholarly journals Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 849-855 ◽  
Author(s):  
Kadriye Inan ◽  
Sabriye Canakci ◽  
Ali Osman Belduz ◽  
Fikrettin Sahin
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Gene Sequence Analysis

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus ; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15 : 0 (39.30 %), anteiso-C15 : 0 (26.10 %) and iso-C16 : 0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T ( = DSM 24395T = LMG 26289T).

Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1868-1873 ◽  
Author(s):  
Decui Yin ◽  
Liwei Chen ◽  
Jingqun Ao ◽  
Chunxiang Ai ◽  
Xinhua Chen
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Moderate Thermophile ◽  
Terrestrial Hot Spring

An aerobic, motile, moderately thermophilic rod, designated strain CLWT, was isolated from a terrestrial hot spring in an exposition garden in Xiamen City, Fujian Province, the People’s Republic of China. Strain CLWT formed beige, dry colonies on solid 2216E medium and flocks in liquid medium. Cells were Gram-stain-negative, short rods (1.0–3.0 µm long and 0.4–0.6 µm wide) with six or more polar flagella. The temperature and pH for growth of strain CLWT were 28–65 °C (optimum, 50–58 °C) and pH 5.5–9.5 (optimum, pH 6.0–8.0). Growth occurred in the presence of 0.3–6.0 % NaCl (optimum 2.5–4.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the closest relative of the isolate was Amaricoccus kaplicensis Ben 101T (94.3 % sequence similarity). The DNA G+C content of strain CLWT was 72.2 mol%. The respiratory quinone was ubiquinone 10. The predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>10 %) were summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c), C18 : 1ω7c 11-methyl and C18 : 0. Based on phylogenetic, physiological and biochemical data and DNA G+C content, strain CLWT is considered to represent a novel species of a new genus in the family Rhodobacteraceae , for which the name Pleomorphobacterium xiamenense gen. nov., sp. nov. is proposed. The type strain of the type species is CLWT ( = LMG 26245T = CGMCC 1.10808T = MCCC 1A06272T).

Albidovulum xiamenense sp. nov., a moderately thermophilic bacterium from a terrestrial hot spring

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1609-1612 ◽  
Author(s):  
Decui Yin ◽  
Jing Xiao ◽  
Jingqun Ao ◽  
Chunxiang Ai ◽  
Xinhua Chen
Keyword(s):  
Phylogenetic Analysis ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Republic Of China ◽  
Link Type ◽  
Terrestrial Hot Spring

An aerobic, motile, moderately thermophilic, rod-shaped bacterium, strain YBY-7T, was isolated from a terrestrial hot spring of a garden exhibition located in Xiamen City, Fujian Province, People’s Republic of China. Cells of strain YBY-7T were Gram-negative, irregular rods, 2–6 µm long and 0.4–0.6 µm wide, with polar flagella, and the organism formed beige colonies. The temperature and pH ranges for growth of strain YBY-7T were 28–65 °C (optimum 50–58 °C) and pH 6.5–9.5 (optimum pH 7.5–8.5). Growth occurred in the presence of 5.5 % NaCl (optimum 3.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that Albidovulum inexpectatum FRR-10T was its closest neighbour (95.9 % similarity). Ubiquinone (Q-10) was the sole respiratory quinone and the DNA G+C content of strain YBY-7T was 70.6 mol%. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were C18 : 1ω7c (29.3 %), C19 : 0 cyclo ω8c (25.6 %), C18 : 0 (23.6 %) and C16 : 0 (9.6 %). Based on its physiological characteristics and our phylogenetic analysis, we propose that strain YBY-7T represents a novel species of the genus Albidovulum , for which the name Albidovulum xiamenense sp. nov. is proposed, with the type strain YBY-7T ( = MCCC 1A06317T  = CGMCC 1.10789T  = LMG 26247T).

Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 109-113 ◽  
Author(s):  
Zhao-Ming Gao ◽  
Xin Liu ◽  
Xi-Ying Zhang ◽  
Ling-Wei Ruan
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Anaerobic Bacterium ◽  
Hot Spring ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
The Family

A moderately thermophilic and strictly anaerobic bacterium, designated HS1T, was isolated from offshore hot spring sediment in Xiamen, China. Cells were Gram-negative, catalase-positive, oxidase-negative, slender and flexible rods without flagella. The strain could grow at 35–55 °C (optimum at 50 °C) and in 1–8 % NaCl (w/v; optimum 2–4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HS1T was affiliated with the family Marinilabiliaceae and shared a distant relationship with the previously described genera. The isolate was most closely related to Anaerophaga thermohalophila Fru22T with 16S rRNA gene sequence similarity of 92.4 %, followed by the other members of the family Marinilabiliaceae with 88.7–91.1 % similarity. The dominant cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The predominant quinone was MK-7. The major polar lipids were phosphatidylethanolamine (PE) and an unknown polar lipid. The genomic DNA G+C content was 38.7 mol%. Besides the phylogenetically distant relationship, strain HS1T was obviously distinguished from the most closely related genera in several phenotypic properties including colony colour and pigment production, optimal temperature, optimal NaCl, relation to O2, bicarbonate/carbonate requirement, catalase activity, nitrate reduction, fermentation products and cellular fatty acid profile. Based on the phenotypic and phylogenetic data, strain HS1T represents a novel species of a new genus, for which the name Thermophagus xiamenensis gen. nov., sp. nov. is proposed. The type strain of the type species is HS1T ( = DSM 19012T = CGMCCC 1.5071T).

Fonticella tunisiensis gen. nov., sp. nov., isolated from a hot spring

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 1947-1950 ◽  
Author(s):  
Belkis Fraj ◽  
Wajdi Ben Hania ◽  
Anne Postec ◽  
Moktar Hamdi ◽  
Bernard Ollivier ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Fermentation Products ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Nitrate And Nitrite

A strictly anaerobic, moderately thermophilic, halotolerant rod, designated BELH25T, was isolated from a water sample of a Tunisian hot spring. Cells were non-motile, 2–6 µm long and 0.4–0.6 µm wide, appearing singly or in pairs. The isolate grew at 45–70 °C (optimum 55 °C), at pH 6.2–8.0 (optimum pH 7.0) and with 0–4 % NaCl (optimum 0–2.0 %). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25T used cellobiose, fructose, galactose, glucose, maltose, mannose, sucrose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO2. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 and anteiso-C15 : 0. The DNA G+C content was 37.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain BELH25T was most closely related to Caloramator viterbiensis JW/MS-VS5T and Fervidicella metallireducens AeBT (92.2 and 92.1 % sequence similarity, respectively), and the isolate was positioned approximately equidistantly between these genera. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain BELH25T is proposed to be a member of a novel species of a novel genus within the order Clostridiales , family Clostridiaceae , for which the name Fonticella tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is BELH25T ( = DSM 24455T = JCM 17559T).

Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 479-483 ◽  
Author(s):  
Anna A. Perevalova ◽  
Ilya V. Kublanov ◽  
R. V. Baslerov ◽  
Gengxin Zhang ◽  
Elizaveta A. Bonch-Osmolovskaya
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Content Type ◽  
Positive Type ◽  
Link Type ◽  
Terrestrial Hot Spring

A novel thermophilic bacterium, strain Kam1851T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851T were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5–8.5. The optimal growth (doubling time, 6.0 h) was at 60–65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C16 : 0 (34.2 %), iso-C16 : 0 (18 %), C18 : 0 (12.8 %) and iso-C17 : 0 (11.1 %). The G+C content of the genomic DNA of strain Kam1851T was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851T belonged to the order Thermoanaerobacterales , but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2 % sequence similarity, were members of the genera Ammonifex and Caldanaerobacter . On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851T is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851T ( = DSM 22653T = VKM B-2685T).

scholarly journals Tepidisphaera mucosa gen. nov., sp. nov., a moderately thermophilic member of the class Phycisphaerae in the phylum Planctomycetes, and proposal of a new family, Tepidisphaeraceae fam. nov., and a new order, Tepidisphaerales ord. nov.

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 549-555 ◽  
Author(s):  
O. L. Kovaleva ◽  
A. Yu. Merkel ◽  
A. A. Novikov ◽  
R. V. Baslerov ◽  
S. V. Toshchakov ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Hot Springs ◽  
Thermophilic Bacteria ◽  
Physiological Data ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type

Three strains of facultatively aerobic, moderately thermophilic bacteria were isolated from terrestrial hot springs in Baikal Lake region and Kamchatka (Russia). Cells of the new isolates were cocci reproducing by binary fission. The temperature range for growth was between 20 and 56 °C and the pH range for growth from pH 4.5 to 8.5, with optimal growth at 47–50 °C and pH 7.0–7.5. The organisms were chemoheterotrophs preferring sugars and polysaccharides as growth substrates. 16S rRNA gene sequences of strains 2842, 2813 and 2918Kr were nearly identical (99.7–100 % similarity) and indicated that the strains belonged to the phylum Planctomycetes . The phylogenetically closest cultivated relatives were Algisphaera agarilytica 06SJR6-2T and Phycisphaera mikurensis FYK2301M01T with 16S rRNA gene sequence similarity values of 82.4 and 80.3 %, respectively. The novel strains differed from them by higher growth temperature, sensitivity to NaCl concentration above 3.0 % and by their cellular fatty acids profile. On the basis of phylogenetic and physiological data, strains 2842T, 2813 and 2918Kr represent a novel genus and species for which we propose the name Tepidisphaera mucosa sp. nov. The type strain is 2842T ( = VKM B-2832T = JCM 19875T). We also propose that Tepidisphaera gen. nov. is the type genus of a novel family, Tepidisphaeraceae fam. nov. and a novel order, Tepidisphaerales ord. nov.

Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4064-4071 ◽  
Author(s):  
Xin-Qi Zhang ◽  
Zhen-Li Zhang ◽  
Nan Wu ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Hot Spring ◽  
Novel Species ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Bacterium Strain

A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4T, was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4T grew at 38–66 °C (optimum 57–60 °C), at pH 6.0–9.3 (optimum 7.0–7.5) and with 0–4 % (w/v) NaCl (optimum 0–0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA–DNA relatedness values, indicated that the isolate represents a novel species of the genus Anoxybacillus , related most closely to Anoxybacillus voinovskiensis DSM 12111T. Strain 3nP4T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C15 : 0 and iso-C17 : 0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus . The mean DNA G+C content of strain 3nP4T was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4T is considered to represent a novel species of the genus Anoxybacillus , for which the name Anoxybacillus vitaminiphilus sp. nov. is proposed. The type strain is 3nP4T ( = CGMCC 1.8979T = JCM 16594T).

Taxonomic study of the genus Tepidiphilus: transfer of Petrobacter succinatimandens to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov., emended description of the genus Tepidiphilus and description of Tepidiphilus thermophilus sp. nov., isolated from a terrestrial hot spring

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 228-235 ◽  
Author(s):  
Abhijit Poddar ◽  
Rinchen T. Lepcha ◽  
Subrata K. Das
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Hot Spring ◽  
Rrna Gene ◽  
Content Type ◽  
Dna Relatedness ◽  
Link Type ◽  
Terrestrial Hot Spring ◽  
Type Strains

Comparative phenotypic, chemotaxonomic and genetic analysis revealed significant similarities among strains of the genera Tepidiphilus and Petrobacter . Analysis of 16S rRNA gene sequences and DNA–DNA relatedness of the type strains Tepidiphilus margaritifer N2-214T and Petrobacter succinatimandens 4BONT showed sequence similarity of 98.9 % and less than 40 % relatedness, indicating that these strains represent different species of same genus. Both strains had phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol as major polar lipids. Their fatty acid profiles were almost identical, with the predominant fatty acids C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. In view of this, we propose to transfer the member of the genus Petrobacter to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov. and to emend the description of the genus Tepidiphilus . Further, a novel bacterium, strain JHK30T, was isolated from a terrestrial hot spring located at Jharkhand, India, and was identified following a polyphasic approach. Cells were non-sporulating, aerobic, Gram-stain-negative rods and motile by a single polar flagellum. Optimum temperature for growth was 50–55 °C at pH 6.5–7.0. 16S rRNA gene sequence analysis revealed 99.71 % similarity with P. succinatimandens 4BONT ( = DSM 15512T) and 98.71 % with T. margaritifer N2-214T ( = DSM 15129T). However, DNA–DNA relatedness of strain JHK30T with these two type strains was well below 70 %. The DNA G+C base composition was 66.1 mol%. Strain JHK30T represents a novel species of the genus Tepidiphilus for which the name Tepidiphilus thermophilus sp. nov. is proposed. The type strain is JHK30T ( = JCM 19170T = LMG 27587T= DSM 27220T).

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