Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 479-483 ◽  
Author(s):  
Anna A. Perevalova ◽  
Ilya V. Kublanov ◽  
R. V. Baslerov ◽  
Gengxin Zhang ◽  
Elizaveta A. Bonch-Osmolovskaya
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Content Type ◽  
Positive Type ◽  
Link Type ◽  
Terrestrial Hot Spring

A novel thermophilic bacterium, strain Kam1851T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851T were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5–8.5. The optimal growth (doubling time, 6.0 h) was at 60–65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C16 : 0 (34.2 %), iso-C16 : 0 (18 %), C18 : 0 (12.8 %) and iso-C17 : 0 (11.1 %). The G+C content of the genomic DNA of strain Kam1851T was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851T belonged to the order Thermoanaerobacterales , but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2 % sequence similarity, were members of the genera Ammonifex and Caldanaerobacter . On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851T is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851T ( = DSM 22653T = VKM B-2685T).

Caloramator quimbayensis sp. nov., an anaerobic, moderately thermophilic bacterium isolated from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1396-1402 ◽  
Author(s):  
Carolina Rubiano-Labrador ◽  
Sandra Baena ◽  
Carolina Díaz-Cárdenas ◽  
Bharat K. C. Patel
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Glucose Fermentation ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Terrestrial Hot Spring

An anaerobic, moderately thermophilic, terminal-spore-forming bacterium, designated strain USBA AT, was isolated from a terrestrial hot spring located at an altitude of 2683 m in the Andean region of Colombia (04° 50′ 14.0″ N 75° 32′ 53.4″ W). Cells of strain USBA AT were Gram-stain-positive, straight to slightly curved rods (0.9×2.5 µm), that were arranged singly or in pairs, and were motile by means of flagella. Growth occurred at 37–55 °C and pH 6.0–8.0, with a doubling time of 2 h under the optimal conditions (50 °C and pH 7.0). Glucose fermentation in strain USBA AT required yeast extract or peptone (each at 0.2 %, w/v). The novel strain fermented sugars, amino acids, Casamino acids, propanol, propionate, starch and dextrin, but no growth was observed on galactose, lactose, xylose, histidine, serine, threonine, benzoate, butyrate, lactate, pyruvate, succinate, methanol, ethanol, glycerol, casein, gelatin or xylan. The end products of glucose fermentation were formate, acetate, ethanol and lactate. Strain USBA AT did not grow autotrophically (with CO2 as carbon source and H2 as electron donor) and did not reduce thiosulfate, sulfate, elemental sulfur, sulfite, vanadium (V) or Fe (III) citrate. Growth of strain USBA AT was inhibited by ampicillin, chloramphenicol, kanamycin, penicillin and streptomycin (each at 10 µg ml−1). The predominant fatty acids were iso-C15 : 0, C16 : 0 and iso-C17 : 0 and the genomic DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis indicated that strain USBA AT belonged in the phylum Firmicutes and that its closest relative was Caloramator viterbiensis JW/MS-VS5T (95.0 % sequence similarity). A DNA–DNA relatedness value of only 30 % was recorded in hybridization experiments between strain USBA AT and Caloramator viterbiensis DSM 13723T. Based on the phenotypic, chemotaxonomic and phylogenetic evidence and the results of the DNA–DNA hybridization experiments, strain USBA AT represents a novel species of the genus Caloramator , for which the name Caloramator quimbayensis sp. nov. is proposed. The type strain is USBA AT ( = CMPUJ U833T  = DSM 22093T).

scholarly journals Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 849-855 ◽  
Author(s):  
Kadriye Inan ◽  
Sabriye Canakci ◽  
Ali Osman Belduz ◽  
Fikrettin Sahin
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Gene Sequence Analysis

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus ; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15 : 0 (39.30 %), anteiso-C15 : 0 (26.10 %) and iso-C16 : 0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T ( = DSM 24395T = LMG 26289T).

Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1868-1873 ◽  
Author(s):  
Decui Yin ◽  
Liwei Chen ◽  
Jingqun Ao ◽  
Chunxiang Ai ◽  
Xinhua Chen
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Moderate Thermophile ◽  
Terrestrial Hot Spring

An aerobic, motile, moderately thermophilic rod, designated strain CLWT, was isolated from a terrestrial hot spring in an exposition garden in Xiamen City, Fujian Province, the People’s Republic of China. Strain CLWT formed beige, dry colonies on solid 2216E medium and flocks in liquid medium. Cells were Gram-stain-negative, short rods (1.0–3.0 µm long and 0.4–0.6 µm wide) with six or more polar flagella. The temperature and pH for growth of strain CLWT were 28–65 °C (optimum, 50–58 °C) and pH 5.5–9.5 (optimum, pH 6.0–8.0). Growth occurred in the presence of 0.3–6.0 % NaCl (optimum 2.5–4.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the closest relative of the isolate was Amaricoccus kaplicensis Ben 101T (94.3 % sequence similarity). The DNA G+C content of strain CLWT was 72.2 mol%. The respiratory quinone was ubiquinone 10. The predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>10 %) were summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c), C18 : 1ω7c 11-methyl and C18 : 0. Based on phylogenetic, physiological and biochemical data and DNA G+C content, strain CLWT is considered to represent a novel species of a new genus in the family Rhodobacteraceae , for which the name Pleomorphobacterium xiamenense gen. nov., sp. nov. is proposed. The type strain of the type species is CLWT ( = LMG 26245T = CGMCC 1.10808T = MCCC 1A06272T).

Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 254-259 ◽  
Author(s):  
Nathaniel A. Losey ◽  
Bradley S. Stevenson ◽  
Susanne Verbarg ◽  
Stephen Rudd ◽  
Edward R. B. Moore ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
New Family ◽  
Terrestrial Hot Spring

A novel bacterial strain designated HA-01T was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rod-shaped, aerobic, chemo-organotrophic, oxidase- and catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37–60 °C, with an optimum between 45 and 50 °C, and at pH 6.5–8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01T were Solimonas aquatica NAA-16T (93.8 %), Solimonas flava CW-KD 4T (94.1 %), Solimonas soli DCY12T (93.1 %), Solimonas variicoloris MN28T (94.0 %), Nevskia ramosa Soe1T (91.2 %) and Hydrocarboniphaga effusa AP103T (91.1 %). Major fatty acids consisted of C16 : 0, iso-C16 : 0, C16 : 1ω5c and summed feature 8 (C18 : 1ω9c, C18 : 1ω7c and C18 : 1ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01T represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01T ( = DSM 23609T = CCUG 59713T). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.

Albidovulum xiamenense sp. nov., a moderately thermophilic bacterium from a terrestrial hot spring

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1609-1612 ◽  
Author(s):  
Decui Yin ◽  
Jing Xiao ◽  
Jingqun Ao ◽  
Chunxiang Ai ◽  
Xinhua Chen
Keyword(s):  
Phylogenetic Analysis ◽  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Republic Of China ◽  
Link Type ◽  
Terrestrial Hot Spring

An aerobic, motile, moderately thermophilic, rod-shaped bacterium, strain YBY-7T, was isolated from a terrestrial hot spring of a garden exhibition located in Xiamen City, Fujian Province, People’s Republic of China. Cells of strain YBY-7T were Gram-negative, irregular rods, 2–6 µm long and 0.4–0.6 µm wide, with polar flagella, and the organism formed beige colonies. The temperature and pH ranges for growth of strain YBY-7T were 28–65 °C (optimum 50–58 °C) and pH 6.5–9.5 (optimum pH 7.5–8.5). Growth occurred in the presence of 5.5 % NaCl (optimum 3.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that Albidovulum inexpectatum FRR-10T was its closest neighbour (95.9 % similarity). Ubiquinone (Q-10) was the sole respiratory quinone and the DNA G+C content of strain YBY-7T was 70.6 mol%. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were C18 : 1ω7c (29.3 %), C19 : 0 cyclo ω8c (25.6 %), C18 : 0 (23.6 %) and C16 : 0 (9.6 %). Based on its physiological characteristics and our phylogenetic analysis, we propose that strain YBY-7T represents a novel species of the genus Albidovulum , for which the name Albidovulum xiamenense sp. nov. is proposed, with the type strain YBY-7T ( = MCCC 1A06317T  = CGMCC 1.10789T  = LMG 26247T).

scholarly journals Rhodobacter flagellatus sp. nov., a thermophilic bacterium isolated from a hot spring

2020 ◽  
Vol 70 (3) ◽  
pp. 1541-1546 ◽  
Author(s):  
Wen-Dong Xian ◽  
Ze-Tao Liu ◽  
Meng-Meng Li ◽  
Lan Liu ◽  
Yu-Zhen Ming ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Intracytoplasmic Membrane ◽  
Respiratory Quinone ◽  
Content Type ◽  
Phenotypic Characters ◽  
Link Type

A thermophilic bacterium, designated SYSU G03088T, was isolated from Moincer hot spring, Tibet, PR China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomic position and genomic profiles of the strain. Phylogenetic analysis using 16S rRNA gene sequence indicated that SYSU G03088T showed highest sequence similarity to Rhodobacter blasticus CGMCC 1.3365T (96.0 % sequence identity). The strain could be differentiated from most recognized species of the genus Rhodobacter by its slightly purple colony colour, distinct phenotypic characters and low ANI values. Cells were Gram-staining negative, and oval-to-rod shaped. Poly-β-hydroxybutyrate and vesicular intracytoplasmic membrane structures were formed inside cells. Growth occurred optimally at 45 °C and pH 7.0. Ubiquinone 10 was the only respiratory quinone. The major fatty acids (>10 %) were C18 : 0, C18 : 1ω7c 11-methyl and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The detected polar lipids of SYSU G03088T included diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The DNA G+C content of SYSU G03088T was 67.7 % (genome). On the basis of the differences in the phenotypic characteristics and phylogenetic analyses, SYSU G03088T is considered to represent a novel species of the genus Rhodobacter , for which the name Rhodobacter flagellatus sp. nov. is proposed. The type strain is SYSU G03088T (=CGMCC 1.16876T=KCTC 72354T).

Litorilinea aerophila gen. nov., sp. nov., an aerobic member of the class Caldilineae , phylum Chloroflexi , isolated from an intertidal hot spring

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1149-1154 ◽  
Author(s):  
Varsha Kale ◽  
Snædís H. Björnsdóttir ◽  
Ólafur H. Friðjónsson ◽  
Sólveig K. Pétursdóttir ◽  
Sesselja Ómarsdóttir ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Phenotypic Differences ◽  
Link Type ◽  
Fermentative Growth ◽  
Distinct Lineage ◽  
Ph Range

A thermophilic, aerobic, Gram-stain-negative, filamentous bacterium, strain PRI-4131T, was isolated from an intertidal hot spring in Isafjardardjup, NW Iceland. The strain grew chemo-organotrophically on various carbohydrates. The temperature range for growth was 40–65 °C (optimum 55 °C), the pH range was pH 6.5–9.0 (optimum pH 7.0) and the NaCl range was 0–3 % (w/v) (optimum 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRI-4131T represented a distinct lineage within the class Caldilineae of the phylum Chloroflexi. The highest levels of sequence similarity, about 91 %, were with Caldilinea aerophila STL-6-O1T and Caldilinea tarbellica D1-25-10-4T. Fermentative growth was not observed for strain PRI-4131T, which, in addition to other characteristics, distinguished it from the two Caldilinea species. Owing to both phylogenetic and phenotypic differences from the described members of the class Caldilineae , we propose to accommodate strain PRI-4131T in a novel species in a new genus, Litorilinea aerophila gen. nov., sp. nov. The type strain of Litorilinea aerophila is PRI-4131T ( = DSM 25763T  = ATCC BAA-2444T).

Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum

2014 ◽  
Vol 64 (Pt_2) ◽  
pp. 501-505 ◽  
Author(s):  
Jonathan Kennedy ◽  
Lekha Menon Margassery ◽  
Niall D. O’Leary ◽  
Fergal O’Gara ◽  
John Morrissey ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Phenotypic Differences ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
The Family

A Gram-stain-negative, rod-shaped, orange-coloured, catalase- and oxidase-positive, non-motile bacterium, designated strain 92VT, was isolated from the marine sponge Amphilectus fucorum, collected from Lough Hyne, County Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92VT clustered with members of the family Flavobacteriaceae , the closest member being Aquimarina latercula NCIMB 1399T, with a gene sequence similarity of 97.5 %. Strain 92VT required seawater for growth with optimal growth occurring at 25 °C, at pH 6–7 and with 3 % (w/v) NaCl. MK-6 was the sole respiratory quinone present and the major fatty acids were iso-C17 : 0 3-OH, iso-C15 : 0, iso-C17 : 1ω9c and iso-C15 : 0 3-OH. The DNA G+C content was 36.1 mol%. Combined phenotypic differences and phylogenetic analysis indicate that strain 92VT represents a novel species of the genus Aquimarina , for which the name Aquimarina amphilecti sp. nov. is proposed. The type strain is 92VT ( = NCIMB 14723T = DSM 25232T).

scholarly journals Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3478-3484 ◽  
Author(s):  
Lars Ganzert ◽  
Janosch Schirmack ◽  
Mashal Alawi ◽  
Kai Mangelsdorf ◽  
Wolfgang Sand ◽  
...  
Keyword(s):  
Type Species ◽  
Dimethyl Sulfide ◽  
Membrane Lipids ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Methanosarcina Barkeri ◽  
Rrna Gene ◽  
Genotypic Differences ◽  
Content Type ◽  
Link Type

A novel methanogenic archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15T were archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species, Methanosarcina vacuolata and Methanosarcina horonobensis, had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15T was similar to those of Methanosarcina vacuolata DSM 1232T (sequence similarity 99.3 %), Methanosarcina horonobensis HB-1T (98.8 %), Methanosarcina barkeri DSM 800T (98.7 %) and Methanosarcina siciliae T4/MT (98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15T and Methanosarcina vacuolata DSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15T represents a novel species of the genus Methanosarcina , for which the name Methanosarcina spelaei sp. nov. is proposed. The type strain is MC-15T ( = DSM 26047T = JCM 18469T).

Methylophaga lonarensis sp. nov., a moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1613-1618 ◽  
Author(s):  
Chakkiath Paul Antony ◽  
Nina V. Doronina ◽  
Rich Boden ◽  
Yuri A. Trotsenko ◽  
Yogesh S. Shouche ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Carbon Assimilation ◽  
Soda Lake ◽  
Optimal Growth ◽  
Cellular Fatty Acid ◽  
Methylotrophic Bacterium ◽  
Vitamin B ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type

A moderately haloalkaliphilic methylotrophic bacterium possessing the ribulose monophosphate pathway for carbon assimilation, designated MPLT, was isolated from Lonar Lake sediment microcosms that were oxidizing methane for two weeks. The isolate utilized methanol and was an aerobic, Gram-negative, asporogenous, motile, short rod that multiplied by binary fission. The isolate required NaHCO3 or NaCl for growth and, although not auxotrophic for vitamin B12, had enhanced growth with vitamin B12. Optimal growth occurred with 0.5–2 % (w/v) NaCl, at 28–30 °C and at pH 9.0–10.0. The cellular fatty acid profile consisted primarily of straight-chain saturated C16 : 0 and unsaturated C16 : 1ω7c and C18 : 1ω7c. The major ubiquinone was Q-8. The dominant phospholipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Cells accumulated ectoine as the main compatible solute. The DNA G+C content was 50.0 mol%. The isolate exhibited 94.0–95.4 % 16S rRNA gene sequence similarity with the type strains of methylotrophs belonging to the genus Methylophaga and 31 % DNA–DNA relatedness with the reference strain, Methylophaga alcalica VKM B-2251T. It is proposed that strain MPLT represents a novel species, Methylophaga lonarensis sp. nov. (type strain MPLT = VKM B-2684T = MCC 1002T).

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