scholarly journals Alloactinosynnema iranicum sp. nov., a rare actinomycete isolated from a hypersaline wetland, and emended description of the genus Alloactinosynnema

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1173-1179 ◽  
Author(s):  
Mahdi Moshtaghi Nikou ◽  
Mohaddaseh Ramezani ◽  
Mohammad Ali Amoozegar ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Peter Schumann ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Emended Description

A Gram-staining-positive actinobacterial strain, Chem10T, was isolated from soil around Inche-Broun hypersaline wetland in the north of Iran. Strain Chem10T was strictly aerobic, and catalase- and oxidase-positive. The isolate grew with 0–3 % NaCl, at 20–40 °C and at pH 6.0–8.0. The optimum temperature and pH for growth were 30 °C and pH 7.0, respectively. The cell wall of strain Chem10T contained meso-diaminopimelic acid as diamino acid and galactose, ribose and arabinose as whole-cell sugars. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain Chem10T synthesized cellular fatty acids of the straight-chain saturated and mono-unsaturated, and iso- and anteiso-branched types C14 : 0, C16 : 0, iso-C16 : 1, anteiso-C17 : 0, iso-C16 : 0, iso-C14 : 0 and iso-C15 : 0, and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Chem10T belonged to the family Pseudonocardiaceae and showed the closest phylogenetic similarity to Alloactinosynnema album KCTC 19294T (98.3 %) and Actinokineospora cibodasensis DSM 45658T (97.9 %). DNA–DNA relatedness values between the novel strain and strains Alloactinosynnema album KCTC 19294T and Actinokineospora cibodasensis DSM 45658T were only 52 % and 23 %, respectively. On the basis of phylogenetic analysis, phenotypic characteristics and DNA–DNA hybridization data, a novel species of the genus Alloactinosynnema is proposed, Alloactinosynnema iranicum sp. nov. The type strain is Chem10T ( = IBRC-M 10403T = CECT 8209T). In addition, an emended description of the genus Alloactinosynnema is proposed.

Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., isolated from surface seawater, and emended description of the genus Sphingomicrobium

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4469-4476 ◽  
Author(s):  
Mariyam Shahina ◽  
Asif Hameed ◽  
Shih-Yao Lin ◽  
Yi-Han Hsu ◽  
You-Cheng Liu ◽  
...  
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Surface Seawater ◽  
Bacterial Strains ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Emended Description

Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30MT and CC-AMZ-30NT, were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30MT shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0 % to Sphingomicrobium lutaoense CC-TBT-3T, Sphingomicrobium astaxanthinifaciens CC-AMO-30BT and other sphingomonads, respectively. Strain CC-AMZ-30NT shared 97.0, 96.7, 95.0 and <95.1 % similarities to strain CC-AMZ-30MT, Sphingomicrobium lutaoense CC-TBT-3T, Sphingomicrobium astaxanthinifaciens CC-AMO-30BT and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained C18 : 1ω7c/C18 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1 2-OH as major (>5 % of the total) fatty acids. Strains CC-AMZ-30MT and CC-AMZ-30NT had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30MT and triamine sym-homospermidine in strain CC-AMZ-30NT. Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C18 : 1 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30MT and CC-AMZ-30NT as two novel species of the genus Sphingomicrobium , for which we propose the names Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., respectively; corresponding type strains are Sphingomicrobium marinum CC-AMZ-30MT ( = JCM 18554T = BCRC 80466T) and Sphingomicrobium flavum CC-AMZ-30NT ( = JCM 18555T = BCRC 80467T). An emended description of the genus Sphingomicrobium is also proposed.

Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 867-872 ◽  
Author(s):  
Herbert Seiler ◽  
Mareike Wenning ◽  
Verena Schmidt ◽  
Siegfried Scherer
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Acid Type

A novel Gram-staining-positive, rod-shaped, motile, strictly aerobic, endospore-forming bacterium, designated WCC 4585T, was isolated from a pharmaceutical production line. The organism grew optimally at 30 °C, at pH 8 and in the presence of 0.5 % (w/v) NaCl. Oval endospores were formed subterminally and terminally in swollen sporangia. The cell-wall diamino acid was meso-diaminopimelic acid (type A1γ) and the genomic DNA G+C content was 38.7 mol%. The major menaquinone was MK-7. The cellular fatty acid profile contained major amounts of iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, and the cellular phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. The isolate was most closely related to Bacillus oceanisediminis H2T, Bacillus infantis SMC 4352-1T, Bacillus firmus NCIMB 9366T, Bacillus circulans ATCC 4513T and Bacillus horneckiae DSM 23495T with which it shared less than 98.0 % 16S rRNA gene sequence similarity. DNA–DNA relatedness values between strain WCC 4585T and five type strains of related species were ≤27 % and sequence similarity values based on groEL sequences were ≤88.7 %. On the basis of the characteristics presented, strain WCC 4585T is proposed to represent a novel species, Bacillus gottheilii sp. nov. The type strain is WCC 4585T( = DSM 23668T = CCUG 59876T = LMG 25856T).

scholarly journals Sneathiella chungangensis sp. nov., isolated from a marine sand, and emended description of the genus Sneathiella

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1468-1472 ◽  
Author(s):  
Chatuphon Siamphan ◽  
Hyungjin Kim ◽  
Jung-Sook Lee ◽  
Wonyong Kim
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
Sand Sample ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Marine Sand ◽  
Emended Description

A Gram-stain-negative, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1294T, was isolated from a sand sample and its taxonomic position was investigated using a polyphasic approach. The strain grew optimally at pH 6.5 and 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1294T formed a lineage with member of the genus Sneathiella and exhibited similarity to Sneathiella glossodoripedis MKT133T (96.3 % similarity) and Sneathiella chinensis LMG 23452T (95.1 % similarity). Strain CAU 1294T contained Q-10 as the predominant respiratory quinone. C18 : 1ω7c, C16 : 0 and cyclo-C19 : 0ω8c were the major cellular fatty acids. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine and two unidentified phospholipids. The DNA G+C content was 56.6 mol%. On the basis of these results, strain CAU 1294T is considered to represent a novel species of the genus Sneathiella , for which the name Sneathiella chungangensis is proposed. The type strain is CAU 1294T ( = KCTC 32476T = CECT 8513T). An emended description of the genus Sneathiella is also proposed.

scholarly journals Roseivivax marinus sp. nov., isolated from deep water

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2540-2544 ◽  
Author(s):  
Xiaofeng Dai ◽  
Xiaochong Shi ◽  
Xin Gao ◽  
Jingli Liu ◽  
Xiao-Hua Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Deep Water ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone

A Gram-stain-negative, strictly aerobic, non-motile, cream, long rod-shaped bacterium, designated strain ZL136T, was isolated from deep water of the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain belonged to the genus Roseivivax with highest sequence similarity to Roseivivax halodurans OCh 239T (97.0 %), followed by Roseivivax isoporae sw-2T (96.9 %). Growth occurred at 4–37 °C (optimum 32 °C), pH 6.0–10.0 (optimum 8.0) and in the presence of 0–12 % (w/v) NaCl (optimum 3–4 %) in marine broth 2216. Strain ZL136T did not produce bacteriochlorophyll a. The predominant fatty acids were C18 : 1ω7c and/or C18 : 1ω6c, C18 : 0, C16 : 0 and 11-methyl C18 : 1ω7c. The major polar lipids of ZL136T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified lipid. The major respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content of strain ZL136T was 67.0 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain ZL136T was classified as a representative of a novel species in the genus of Roseivivax , for which the name Roseivivax marinus sp. nov. is proposed. The type strain is ZL136T ( = JCM 19386T = KCTC 32470T).

Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 21-26 ◽  
Author(s):  
Haneul Kim ◽  
Yochan Joung ◽  
Kiseong Joh
Keyword(s):  
Tidal Flat ◽  
Type Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Emended Description

A Gram-staining-negative, aerobic and rod-shaped bacterial strain, designated HMC4223T, was isolated from a tidal flat in Taean, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMC4223T formed a lineage within the genus Algoriphagus and exhibited highest sequence similarity to Algoriphagus hitonicola 7-UAHT (96.3 %). The major fatty acids of strain HMC4223T were iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c). The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine and five unidentified polar lipids. The DNA G+C content of strain HMC4223T was 48.4 mol%. On the basis of the evidence presented in this study, strain HMC4223T represents a novel species of the genus Algoriphagus , for which the name Algoriphagus taeanensis sp. nov. is proposed, with the type strain HMC4223T ( = KCTC 22709T = NBRC 105728T). An emended description of Algoriphagus hitonicola is also presented.

Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3324-3329 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Maryam Didari ◽  
Maryam Bagheri ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Peter Schumann ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Species ◽  
Novel Species ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type

A Gram-staining-positive, endospore-forming, rod-shaped, strictly aerobic, slightly halophilic bacterium, designated strain A24T, was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain A24T were motile rods and produced oval endospores at a terminal position in swollen sporangia. Strain A24T was catalase and oxidase positive. Growth occurred with between 0.5 and 7.5 % (w/v) NaCl and the isolate grew optimally at 3 % (v/w) NaCl. The optimum temperature and pH for growth were 35 °C and pH 8.0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A24T belonged to the genus Bacillus within the phylum Firmicutes and showed the closest phylogenetic similarity with the species Bacillus alkalitelluris BA288T (97.2 %), Bacillus herbersteinensis D-1,5aT (96.0 %) and Bacillus litoralis SW-211T (95.6 %). The G+C content of the genomic DNA of this strain was 35.9 mol%. The polar lipid pattern of strain A24T consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. The major cellular fatty acids of strain A24T were anteiso-C15 : 0 and iso-C15 : 0. The respiratory quinones were MK-7 (94 %) and MK-6 (4 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate A24T within the genus Bacillus . DNA–DNA hybridization experiments revealed a relatedness of 8 % between strain A24T and Bacillus alkalitelluris IBRC-M 10596T, supporting its placement as a novel species. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus Bacillus , for which the name Bacillus salsus sp. nov. is proposed. The type strain is strain A24T ( = IBRC-M 10078 T = KCTC 13816T).

Gramella aestuarii sp. nov., isolated from a tidal flat, and emended description of Gramella echinicola

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2872-2878 ◽  
Author(s):  
Sang Hyeon Jeong ◽  
Hyun Mi Jin ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Molecular Features ◽  
Gram Staining ◽  
Emended Description

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterial strain motile by gliding, designated BS12T, was isolated from a tidal flat at Boseong, Korea. Cells were moderately halotolerant, catalase- and oxidase-positive rods. Growth was observed at 5–40 °C (optimum, 25 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–11 % (w/v) NaCl (optimum, 2–4 %). The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and anteiso-C15 : 0. The polar lipid pattern comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 42.3 mol% and the only respiratory quinone was menaquinone 6. Phylogenetic inference based on 16S rRNA gene sequences showed that strain BS12T formed a tight phyletic lineage with members of the genus Gramella . Strain BS12T was most closely related to ‘ Gramella jeungdoensis’ HMD3159 with sequence similarity of 97.5 %, but the DNA–DNA relatedness value between the two strains was only 22.1±2.2 %. On the basis of phenotypic and molecular features, strain BS12T was shown to represent a novel species of the genus Gramella , for which the name Gramella aestuarii sp. nov. is proposed. The type strain is BS12T ( = KACC 16188T = JCM 17790T). An emended description of Gramella echinicola is also proposed.

scholarly journals Ramlibacter solisilvae sp. nov., isolated from forest soil, and emended description of the genus Ramlibacter

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1317-1322 ◽  
Author(s):  
Hyo Jung Lee ◽  
Se Hee Lee ◽  
Sang-Suk Lee ◽  
Joon Seon Lee ◽  
Yongseong Kim ◽  
...  
Keyword(s):  
Forest Soil ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Molecular Features ◽  
Gram Staining ◽  
Optimum Ph ◽  
Emended Description

A Gram-staining-negative, strictly aerobic, white-colony-forming bacterium, designated strain 5-10T, was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods or coccoids, showing oxidase- and catalase-positive reactions. Growth was observed at 10–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0–0.5 %). The major cellular fatty acids were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, C10 : 0 3-OH and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c). The G+C content of the genomic DNA was 69.9 mol% and the only respiratory quinone detected was ubiquinone 8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5-10T formed a tight phyletic lineage with members of the genus Ramlibacter . Strain 5-10T was most closely related to Ramlibacter tataouinensis TTB310T (97.3 %), but the DNA–DNA relatedness level between the two strains was 38.2±1.8 %. Based on phenotypic, chemotaxonomic and molecular features, strain 5-10T was shown to represent a novel species of the genus Ramlibacter , for which the name Ramlibacter solisilvae sp. nov. is proposed. The type strain is 5-10T ( = KACC 17567T = JCM 19319T). An emended description of the genus Ramlibacter is also proposed.

Erythrobacter jejuensis sp. nov., isolated from seawater

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1421-1426 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Dong-Heon Lee ◽  
Duck-Chul Oh
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A Gram-staining-negative, yellow-pigmented, non-motile, strictly aerobic, rod-shaped bacterium, designated strain CNU001T, was isolated from seawater collected on the coast of Jeju Island, South Korea, and subjected to a polyphasic taxonomic study. The temperature, pH and NaCl ranges for growth were 10–30 °C, pH 6.0–10.0 and 2.0–5.0 %, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CNU001T belonged to the genus Erythrobacter in the family Erythrobacteraceae , with Erythrobacter longus DSM 6997T (96.6 % sequence similarity), Erythrobacter gaetbuli SW-161T (96.3 %), Erythrobacter vulgaris 022 2-10T (96.2 %), Erythrobacter nanhaisediminis T30T (96.1 %) and other members of the genus Erythrobacter (<96.0 %) identified as the novel strain’s closest relatives. The major cellular fatty acids were C18 : 1ω7c and C17 : 1ω6c. The polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, one sphingoglycolipid, one unidentified aminolipid and six other unidentified lipids. The major respiratory quinone was ubiquinone-10 (UQ-10) and the genomic DNA G+C content of the novel strain was 58.9 mol%. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU001T represents a novel species within the genus Erythrobacter , for which the name Erythrobacter jejuensis sp. nov. is proposed. The type strain is CNU001T ( = KCTC 23090T  = JCM 16677T).

Kordia aquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4790-4796 ◽  
Author(s):  
Asif Hameed ◽  
Mariyam Shahina ◽  
Shih-Yao Lin ◽  
Jang-Cheon Cho ◽  
Wei-An Lai ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Surface Seawater ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301T was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301T predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412T (95.0 %), K. algicida KCTC 8814PT (94.4 %) and K. antarctica IMCC3317T (94.2 %), and formed a phylogenetic lineage tightly associated with species of the genus Kordia . The polar lipid profile of strain CC-AMZ-301T consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5 % of total) were iso-C17 : 0 3-OH, iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301T is proposed to represent a novel species of the genus Kordia , for which the name Kordia aquimaris sp. nov. is proposed; the type strain is CC-AMZ-301T ( = JCM 18556T = BCRC 80464T). An emended description of the genus Kordia is also proposed.

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