scholarly journals Two novel Saccharomycopsis species isolated from black olive brines and a tropical plant. Description of Saccharomycopsis olivae f. a., sp. nov. and Saccharomycopsis guyanensis f. a., sp. nov. Reassignment of Candida amapae to Saccharomycopsis amapae f. a., comb. nov., Candida lassenensis to Saccharomycopsis lassenensis f. a., comb. nov. and Arthroascus babjevae to Saccharomycopsis babjevae f. a., comb. nov.

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2169-2175 ◽  
Author(s):  
Noémie Jacques ◽  
Christelle Louis-Mondesir ◽  
Monika Coton ◽  
Emmanuel Coton ◽  
Serge Casaregola
Keyword(s):  
Type Strain ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Tropical Plant ◽  
Black Olive ◽  
Novel Taxa ◽  
Large Subunit Rrna

Three yeast strains related to members of the genus Saccharomycopsis were isolated. One strain (CLIB 1310) was isolated from olive brines of fermented black olives in France and two strains (CLIB 1454 and CLIB 1455) were isolated from a plant in French Guiana. Sequence analyses based on the D1/D2 domains of the nuclear large subunit rRNA gene, small-subunit rRNA gene and partial EF-1α gene revealed that the strains represented two novel taxa exhibiting extensive sequence divergence from the previously described species of the genus Saccharomycopsis. Two novel species are described to accommodate these newly isolated strains: Saccharomycopsis olivae sp. nov. (type strain CLIB 1310T = CBS 12701T) and Saccharomycopsis guyanensis sp. nov. (type strain CLIB 1455T = CBS 12914T and strain CLIB 1454). Both strains CLIB 1454 and CLIB 1455T displayed identical sequences but differed in their ability to metabolize sorbitol and in their morphology on agar medium. Candida amapae, Candida lassensensis and Arthroascus babjevae belonging to the Saccharomycopsis clade, are reassigned to Saccharomycopsis as novel combinations.

Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 543-556 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin M. Giblin-Davis ◽  
Changhui Li ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Wasp Species ◽  
Dna Sequence Data ◽  
Ficus Hirta ◽  
Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

Candida ficus sp. nov., a novel yeast species from the gut of Apriona germari larvae

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2805-2809 ◽  
Author(s):  
Feng-Li Hui ◽  
Qiu-Hong Niu ◽  
Tao Ke ◽  
Zheng Liu
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Central China ◽  
Rrna Gene ◽  
The Novel ◽  
Ascomycetous Yeasts ◽  
Large Subunit Rrna ◽  
Wood Boring

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of Ficus carica cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of Pichia terricola, and by 15.8 % divergence from the type strains of Pichia exigua and Candida rugopelliculosa in the D1/D2 domains. All three are ascomycetous yeasts in the Pichia clade. Unlike P. terricola, P. exigua and C. rugopelliculosa, the novel isolates did not ferment glucose. The name Candida ficus sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8T ( = CICC 1980T = CBS 12638T) as the type strain.

Ficophagus auriculatae n. sp. (Nematoda: Aphelenchoididae), an associate of the Ficus auriculata-complex in China

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 497-508
Author(s):  
Yongsan Zeng ◽  
Jianfeng Huang ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Rongrong Shi ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Small Subunit Rrna Gene ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus auriculata-complex, including F. auriculata, F. oligodon and F. hainanensis, from Xishuangbanna Tropical Botanical Garden, China. It is described herein as Ficophagus auriculatae n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located between the metacorpus and the stylet basal knobs, testis multi-reflexed, amoeboid sperm, three pairs of subventral papillae on male tail, rounded male tail tip without mucron, absence of gubernaculum (= apophysis) and sickle-shaped spicules with terminal cucullus. Ficophagus auriculatae n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analysis with the above-mentioned sequences suggested that F. auriculatae n. sp. has a closer relationship with F. fleckeri in the same highly supported monophyletic clade than with the other sequenced Ficophagus and Schistonchus sensu lato species.

Phylogeny of mitosporic entomopathogenic fungi: Is the genus Paecilomyces polyphyletic?

2001 ◽  
Vol 47 (9) ◽  
pp. 813-819 ◽  
Author(s):  
Miroslav Oborník ◽  
Milan Jirku ◽  
David Dolezel
Keyword(s):  
Ribosomal Rna ◽  
Entomopathogenic Fungi ◽  
Large Subunit ◽  
Sister Group ◽  
Small Subunit ◽  
Verticillium Lecanii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Large Subunit Rrna ◽  
Rna Genes

We analyzed sequences of the divergent domain at the 5' end of the large subunit rRNA gene from the mitosporic entomopathogenic fungi Paecilomyces sp., Paecilomyces fumosoroseus, Paecilomyces farinosus, Paecilomyces lilacinus, Verticillium lecanii, Verticillium psalliotae, Beauveria bassiana, Aschersonia sp., Aschersonia placenta, ascomycetous Cordyceps sp., and Cordyceps militaris. Phylogenetic analysis showed P. fumosorseus as the best characterized out of the analyzed species with the B. bassiana clade as its sister group. Two of the P. farinosus isolates were invariably placed within the Verticillium cluster, which also contained C. militaris. The only analyzed P. lilacinus isolate appeared on the root of the hyphomycetous fungi and was characterized as the most distinct from all the hyphomycetous fungi tested. Polyphyly of the genus Paecilomyces was well supported by the Kishino-Hasegawa test. In all trees based on the small subunit rRNA gene sequences obtained from the GenBank(tm), V. lecanii, V. psalliotae, P. fumosoroseus, P. tenuipes and B. bassiana form, together with that of C. militaris, the best supported cluster in the tree. The rest of Cordyceps spp. constitute a distinct clade. Phylogenetic relationships derived from both tested DNA regions show polyphyly of the genus Paecilomyces and close relationships among entomopathogenic species of the genera Verticillium, Paecilomyces, and Beauveria.Key words: Paecilomyces, Verticillium, Beauveria, Aschersonia, entomopathogenic fungi, molecular phylogeny, ribosomal RNA genes.

Description of Schistonchus superbus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus superba in China

Nematology ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 771-781 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Changhui Li ◽  
Xinrong Wang ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Academy Of Sciences ◽  
Large Subunit Rrna ◽  
Nematode Biodiversity

A new nematode species was recovered from the syconia of Ficus superba from the South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong Province, China, during a survey of nematode biodiversity from 2007 to 2012. It is described herein as Schistonchus superbus n. sp. and is characterised by possessing the combined characters of a short post-uterine sac and digitate tail without a mucron in females, excretory pore located near the metacorpus, amoeboid sperm, a unique wedge-shaped male tail with variable tip and two pairs of subventral papillae, absence of a gubernaculum and possession of sickle-shaped spicules without a cucullus and with a thorn-shaped rostrum. Schistonchus superbus n. sp. is typologically differentiated from all other described species in this genus by the possession of a wedge-shaped tail in males and a digitate tail without mucron in females. Schistonchus superbus n. sp. was easily differentiated from other sequenced species by the partial small subunit rRNA gene (SSU), D3 expansion segment of the large subunit rRNA gene (LSU) and mitochondrial DNA subunit I (mtCOI). Phylogenetic analysis with partial SSU sequences suggests that S. superbus n. sp. is sister to S. caprifici and S. hirtus, and is a polyphyletic relative to other Schistonchus species that have been sequenced. Based on inferences using LSU D3 sequence data, S. superbus n. sp. is in a well-supported monophyletic clade with S. caprifici, S. hirtus and S. macrophylla.

Ficophagus curtipes n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus curtipes in China

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1091-1101
Author(s):  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
Yanqiong Peng ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus curtipes from Xishuangbanna Tropical Botanical Garden, Yunnan, China. It is described herein as Ficophagus curtipes n. sp. and is differentiated from all currently described species in the genus by the possession of a spicule with a ventrally recurved tip (terminal barb). It is further characterised by possessing a short post-uterine sac, an ovoid spermatheca, presence of crustaformeria, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip without mucron, rose-thorn-shaped spicules without cucullus and absence of gubernaculum (= apophysis). Ficophagus curtipes n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analyses suggested that F. curtipes n. sp. has a closer relationship with F. benjamina and F. microcarpus in the same highly supported monophyletic clade than to the other sequenced Ficophagus and Schistonchus s.l. species.

scholarly journals Pre-AIDS Era Isolates of Pneumocystis carinii f. sp. hominis: High Genotypic Similarity with Contemporary Isolates

1998 ◽  
Vol 36 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Anthony G. Tsolaki ◽  
Pieter Beckers ◽  
Ann E. Wakefield
Keyword(s):  
Large Subunit ◽  
Pneumocystis Carinii ◽  
Small Subunit ◽  
Its Sequence ◽  
Small Subunit Rrna ◽  
Aids Pandemic ◽  
Genes Encoding ◽  
Lsu Rrna ◽  
Large Subunit Rrna ◽  
Sequence Types

Isolates of Pneumocystis carinii f. sp.hominis were examined from six individuals who died ofP. carinii pneumonia between 1968 and 1981 and who had underlying immunodeficiencies which were not due to human immunodeficiency virus infection. DNA sequence variation was analyzed in the genes encoding the mitochondrial large subunit rRNA (mt LSU rRNA), the internal transcribed spacer (ITS) regions of the nuclear rRNA, the arom locus, and the mitochondrial small subunit rRNA. No major variations were observed when these isolates were compared to isolates from HIV-infected individuals. A small number of minor differences were detected. A new position at which variation occurred in the mt LSU rRNA was observed in one sample. Three new ITS sequence types were identified. A total of nine different ITS sequence types were found in the six samples. Mixed infection with different ITS sequence types of P. carinii f. sp. hominis was observed in four of the six samples. The ITS locus was the most informative of the four loci for distinguishing among the isolates ofP. carinii f. sp. hominis. The data suggest that isolates of P. carinii f. sp. hominis from before the AIDS pandemic are genetically very similar to those currently found in HIV-infected individuals.

scholarly journals Three Small Nucleolar RNAs Identified from the Spliced Leader-Associated RNA Locus in Kinetoplastid Protozoans

1998 ◽  
Vol 18 (8) ◽  
pp. 4409-4417 ◽  
Author(s):  
T. Guy Roberts ◽  
Nancy R. Sturm ◽  
Billy K. Yee ◽  
Michael C. Yu ◽  
Toinette Hartshorne ◽  
...  
Keyword(s):  
Large Subunit ◽  
Small Subunit ◽  
Cell Fractionation ◽  
Small Nucleolar Rnas ◽  
Small Subunit Rrna ◽  
Base Pairs ◽  
Spliced Leader ◽  
Repeat Units ◽  
Large Subunit Rrna ◽  
Nucleolar Rnas

ABSTRACT First characterized in Trypanosoma brucei, the spliced leader-associated (SLA) RNA gene locus has now been isolated from the kinetoplastids Leishmania tarentolae and Trypanosoma cruzi. In addition to the T. brucei SLA RNA, bothL. tarentolae and T. cruzi SLA RNA repeat units also yield RNAs of 75 or 76 nucleotides (nt), 92 or 94 nt, and ∼450 or ∼350 nt, respectively, each with significant sequence identity to transcripts previously described from the T. brucei SLA RNA locus. Cell fractionation studies localize the three additional RNAs to the nucleolus; the presence of box C/D-like elements in two of the transcripts suggests that they are members of a class of small nucleolar RNAs (snoRNAs) that guide modification and cleavage of rRNAs. Candidate rRNA-snoRNA interactions can be found for one domain in each of the C/D element-containing RNAs. The putative target site for the 75/76-nt RNA is a highly conserved portion of the small subunit rRNA that contains 2′-O-ribose methylation at a conserved position (Gm1830) in L. tarentolae and in vertebrates. The 92/94-nt RNA has the potential to form base pairs near a conserved methylation site in the large subunit rRNA, which corresponds to position Gm4141 of small rRNA 2 in T. brucei. These data suggest that trypanosomatids do not obey the general 5-bp rule for snoRNA-mediated methylation.

Characterisation of, and entomopathogenic studies on, Pristionchus aerivorus (Cobb in Merrill & Ford, 1916) Chitwood, 1937 (Rhabditida: Diplogastridae) from North Carolina, USA

Nematology ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 567-580
Author(s):  
Weimin Ye ◽  
Qing Yu ◽  
Natsumi Kanzaki ◽  
Paul R. Adams ◽  
Yasmin J. Cardoza
Keyword(s):  
North Carolina ◽  
Helicoverpa Zea ◽  
Large Subunit ◽  
Small Subunit ◽  
Morphological Observation ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Morphological Studies ◽  
The Status ◽  
Molecular Phylogenetic

During a survey of entomopathogenic nematodes in North Carolina, USA, aPristionchusspecies was recovered using theGalleriabait method. Morphological studies with light microscopy and scanning electron microscopy, mating tests with reference strains, as well as molecular analyses of the near-full-length small subunit rRNA gene (18S) and D2-D3 expansion segments of the large subunit rRNA gene (28S) identified this isolate asPristionchus aerivorus. ExposedGallerialarvae were killed within 48 h and high numbers of nematodes were recovered from the cadavers about 5 days later. Preliminary tests revealed that this nematode is capable of infecting at least two other insect species (Helicoverpa zeaandTenebrio molitor) under laboratory conditions. The status of the genusChroniodiplogasteris discussed and confirmed as a junior synonym ofPristionchusbased on morphological observation and molecular phylogenetic analysis.

Sign in / Sign up

Export Citation Format

Share Document