scholarly journals Hanseniaspora nectarophila sp. nov., a yeast species isolated from ephemeral flowers

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2364-2369 ◽  
Author(s):  
Neža Čadež ◽  
Fernando C. Pagnocca ◽  
Peter Raspor ◽  
Carlos A. Rosa
Keyword(s):  
Novel Species ◽  
Elongation Factor ◽  
Rrna Gene ◽  
The Novel ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Protein Coding ◽  
Yeast Strains ◽  
Close Relationship ◽  
Translation Elongation Factor 1Α

Seven apiculate yeast strains that were isolated from the flowers of Syphocampylus corymbiferus Pohl in Brazil are genetically, morphologically and phenotypically distinct from recognized species of the genera Hanseniaspora and Kloeckera. Genetic discontinuities between the novel strains and their closest relatives were found using a networking approach based on the concatenated sequences of the rRNA gene (internal transcribed spacer and D1/D2 of the LSU), and the protein-coding genes for actin and translation elongation factor-1α. Phylogenetic analysis based on the rRNA and the actin gene placed the novel species represented by the strains in close relationship to Hanseniaspora meyeri and Hanseniaspora clermontiae. PCR fingerprinting with microsatellite primers confirmed the genetic heterogeneity of the novel species. The name Hanseniaspora nectarophila sp. nov. is proposed, with UFMG POG a.1T ( = ZIM 2311T = CBS 13383T) as the type strain; MycoBank no. MB807210. As the current description of the genus does not allow the presence of multilateral budding, an emended diagnosis of the genus Hanseniaspora Zikes is proposed.

scholarly journals Muerte descendente de Prunus sp. asociada a Armillaria spp. en huertas comerciales de Michoacán, México

2017 ◽  
Vol 35 (3) ◽  
Author(s):  
Patricia Rivas-Valencia ◽  
Alejandra Almaraz-Sánchez ◽  
Adriana Cano-Salgado ◽  
Lervin Hernández-Ramos ◽  
Iobana Alanís-Martínez ◽  
...  
Keyword(s):  
Prunus Persica ◽  
Elongation Factor ◽  
Prunus Domestica ◽  
Translation Elongation Factor ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Translation Elongation Factor 1Α

<p>El área productora de durazno (<em>Prunus persica</em>) y ciruelo (<em>Prunus domestica</em>) en Michoacán, se localiza en el municipio de Zinapécuaro. Recientemente se han presentado síntomas de un deterioro progresivo de los árboles que les ocasiona la muerte, poniendo en riesgo la producción de fruta. El objetivo de este estudio fue identificar y caracterizar molecularmente el patógeno responsable. En las localidades de Ucareo, Jeráhuaro, Huajúmbaro, San Joaquín y La Galera con antecedentes del problema, se colectaron muestras de raíz, tronco y suelo. Se identificaron signos característicos del hongo <em>Armillaria</em> spp., confirmado por secuenciación molecular del gen Tef-1α (translation elongation factor 1α). El análisis filogenético agrupó las secuencias como dos especies nuevas, filogenéticamente emparentada en el mismo clado que <em>A. mellea</em>, que es una especie patógena agresiva. En México no se cuenta actualmente con portainjertos tolerantes, y por tanto, se recomienda continuar con estudios de búsqueda de resistencia genética y manejo de la enfermedad en las huertas.</p>

scholarly journals Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nimali I. de Silva ◽  
Alan J. L. Phillips ◽  
Jian-Kui Liu ◽  
Saisamorn Lumyong ◽  
Kevin D. Hyde
Keyword(s):  
New Species ◽  
Base Pair ◽  
Sequence Data ◽  
Elongation Factor ◽  
Internal Transcribed Spacers ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Life Styles ◽  
Forest Plants ◽  
Translation Elongation Factor 1Α

Abstract Two new species of Lasiodiplodia (Lasiodiplodia endophytica and Lasiodiplodia magnoliae) are described and illustrated from Magnolia forests in Yunnan, China. Endophytic and saprobic Lasiodiplodia pseudotheobromae and endophytic L. thailandica are new records from this host. The internal transcribed spacers (ITS), part of the translation elongation factor-1α (tef1) and partial β-tubulin (tub2) sequence data were analyzed to investigate the phylogenetic relationships of the new species with other Lasiodiplodia species. Lasiodiplodia magnoliae is phylogenetically sister to L. mahajangana and L. pandanicola but morphologically distinct from L. mahajangana in having larger conidia. Lasiodiplodia endophytica is most closely related to L. iraniensis and L. thailandica and the three species can be distinguished from one another by 2 base pair differences in ITS and three or four base pair differences in tef1. The new collections suggest that Magnolia forest plants are good hosts for Lasiodiplodia species with endophytic and saprobic life-styles.

scholarly journals Trichosporon xylopini sp. nov., a hemicellulose-degrading yeast isolated from the wood-inhabiting beetle Xylopinus saperdioides

2011 ◽  
Vol 61 (10) ◽  
pp. 2538-2542 ◽  
Author(s):  
Pushpa Gujjari ◽  
Sung-Oui Suh ◽  
Ching-Fu Lee ◽  
Jianlong J. Zhou
Keyword(s):  
Type Strain ◽  
Sole Carbon Source ◽  
Novel Species ◽  
Internal Transcribed Spacers ◽  
Rrna Genes ◽  
Rrna Gene ◽  
The Novel ◽  
Yeast Strains ◽  
Lsu Rrna Gene ◽  
Coleoptera Tenebrionidae

Four arthroconidium-producing yeasts were isolated from the gut of wood-inhabiting tenebrionid and passalid beetles. The rRNA genes of these yeast strains were sequenced, compared and analysed. The sequence results and other taxonomic characterizations placed two of the strains into Trichosporon porosum, and the remaining strains, EH024T and EH026 which were isolated from Xylopinus saperdioides (Coleoptera: Tenebrionidae), into a novel species of the genus Trichosporon in the Porosum clade. Strain EN6S23 was independently isolated from forest soil in Taiwan and was identified as the same novel species based on identical sequences in the internal transcribed spacers (ITS) and the D1/D2 region of the LSU rRNA gene and similar physiological characteristics to those of strains EH024T and EH026. The three strains can assimilate cellulose and xylan as sole carbon source, and are clearly distinguished from their closest taxon, T. porosum, by 14 nt differences in the ITS and D1/D2 region. These strains did not reproduce sexually under the laboratory conditions tested. The novel species is proposed as Trichosporon xylopini sp. nov. (type strain EH024T  = ATCC MYA-4670T  = CBS 11841T).

scholarly journals Identification and Pathogenicity of Fungal Species Associated with Canker Diseases of Pistachio in California

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2397-2411 ◽  
Author(s):  
Mohamed T. Nouri ◽  
Daniel P. Lawrence ◽  
Leslie A. Holland ◽  
David A. Doll ◽  
Craig E. Kallsen ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Schizophyllum Commune ◽  
Elongation Factor ◽  
Fungal Species ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Brown Discoloration ◽  
Pathogenicity Tests ◽  
Branch Dieback ◽  
Translation Elongation Factor 1Α

A survey was conducted during 2015 and 2016 in pistachio orchards throughout the San Joaquin Valley of California to investigate the occurrence of canker diseases and identify the pathogens involved. Cankers and dieback symptoms were observed mainly in orchards aged >15 years. Symptoms of canker diseases included brown to dark brown discoloration of vascular tissues, wood necrosis, and branch dieback. In total, 58 fungal isolates were obtained from cankers and identified based on multilocus phylogenetic analyses (internal transcribed spacer, glyceraldehyde 3-phosphate dehydrogenase, β-tubulin, calmodulin, actin 1, and translation elongation factor 1α) representing 11 fungal species: Colletotrichum karstii, Cytospora californica, Cytospora joaquinensis, Cytospora parapistaciae, Cytospora pistaciae, Diaporthe ambigua, Didymella glomerata, Diplodia mutila, Neofusicoccum mediterraneum, Phaeoacremonium canadense, and Schizophyllum commune. Pathogenicity tests conducted in the main pistachio cultivars Kerman, Golden Hills, and Lost Hills using the mycelium-plug method indicated that all fungal species were pathogenic to Pistacia vera. All species tested caused cankers in pistachio branches, although virulence among species varied from high to moderate. Overall, N. mediterraneum and Cytospora spp. were the most widespread and virulent species associated with canker diseases of pistachio in California.

Kwoniella shandongensis sp. nov., a basidiomycetous yeast isolated from soil and bark from an apple orchard

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2774-2777 ◽  
Author(s):  
Ru Chen ◽  
Yuan-Mao Jiang ◽  
Shao-Chong Wei ◽  
Qi-Ming Wang
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Apple Orchard ◽  
Basidiomycetous Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Yeast Strains ◽  
Its Regions ◽  
Pr China ◽  
26S Rrna

Four basidiomycetous yeast strains (Y13-1T, Y2-1, Y6-3 and Y8-2) were isolated from soil and bark collected from an apple orchard in Tai’an, Shandong province, PR China. Phylogenetic analysis based on 26S rRNA gene D1/D2 domains and ITS regions revealed that these novel strains were located in the Kwoniella clade in the class Tremellomycetes and were closely related to Cryptococcus cuniculi and Kwoniella heveanensis, but were clearly distinct from these species. Therefore, it is proposed that the new strains represent a novel species, Kwoniella shandongensis sp. nov., with the type strain Y13-1T( = CGMCC 2.04458T = CBS 12478T). The MycoBank number for the novel species is MB 564868.

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