scholarly journals Halovivax limisalsi sp. nov., an extremely halophilic archaeon from hypersaline mud

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3422-3426 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Siavash Riazi ◽  
Antonio Ventosa
Keyword(s):  
Type Species ◽  
Optimal Growth ◽  
Rpob Gene ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon ◽  
Ph Range

A Gram-stain-negative, cream‐pigmented, motile, extremely halophilic archaeon, designated strain IC38T, was isolated from a saline mud sample taken from a hypersaline lake, Aran-Bidgol, in Iran. The strain required at least 2.5 M NaCl for growth. However, MgCl2 was not required. Optimal growth occurred with 4.3 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 35 °C, respectively, and strain IC38T was able to grow over a pH range of 6.5–9.0, and a temperature range of 25–45 °C. Analysis of the 16S rRNA gene sequence revealed that strain IC38T clustered with the two species of the genus Halovivax , Halovivax asiaticus EJ-46T and Halovivax ruber XH-70T, with sequence similarities of 96.4 % and 96.1 %, respectively. The similarities between the rpoB′ gene of the novel strain and Halovivax asiaticus and Halovivax ruber were 90.7 % and 90.3 %, respectively. The polar lipid pattern of strain IC38T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Three unidentified glycolipids and two minor phospholipids were also observed. The DNA G+C content of strain IC38T was 62.6 mol%. On the basis of the phylogenetic analysis, as well as the biochemical and physiological characteristics, the new isolate is suggested to be a representative of a novel species of the genus Halovivax , for which the name Halovivax limisalsi sp. nov. is proposed. The type strain of Halovivax limisalsi is IC38T ( = IBRC-M 10022T = KCTC 4051T).

scholarly journals Halovivax cerinus sp. nov., an extremely halophilic archaeon from a hypersaline lake

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 65-70 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Mehrnoosh Rasooli ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
...  
Keyword(s):  
Type Species ◽  
Salt Lake ◽  
Optimal Growth ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
The Novel ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain IC35T, was isolated from a mud sample of the Aran-Bidgol salt lake in Iran. The novel strain was cream, non-motile, rod-shaped and required at least 2.5 M NaCl, but not MgCl2, for growth. Optimal growth was achieved with 3.4 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 (grew over a pH range of 6.5–9.0) and 40 °C (grew over a temperature range of 30–50 °C), respectively. Analysis of 16S rRNA gene sequences revealed that strain IC35T clustered with species of the genus Halovivax , with sequence similarities of 97.3 %, 96.6 % and 96.3 %, respectively, to Halovivax limisalsi IC38T, Halovivax asiaticus EJ-46T and Halovivax ruber XH-70T. The rpoB′ gene similarities between the novel strain and Halovivax limisalsi IBRC-M 10022T, Halovivax ruber JCM 13892T and Halovivax asiaticus JCM 14624T were 90.2 %, 90.2 % and 89.9 %, respectively. The polar lipid pattern of strain IC35T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; six unknown glycolipids and two minor phospholipids were also observed. The only quinone present was MK-8 (II-H2). The G+C content of the genomic DNA was 63.2 mol%. DNA–DNA hybridization studies (29 % hybridization with Halovivax limisalsi IBRC-M 10022T), as well as biochemical and physiological characterization, allowed strain IC35T to be differentiated from other species of the genus Halovivax . A novel species, Halovivax cerinus sp. nov., is therefore proposed to accommodate this strain. The type strain is IC35T ( = IBRC-M 10256T = KCTC 4050T).

Halopiger salifodinae sp. nov., an extremely halophilic archaeon isolated from a salt mine

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3563-3567 ◽  
Author(s):  
Wei-Yan Zhang ◽  
Yuan Meng ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Type Species ◽  
Lipid Analysis ◽  
Rrna Gene ◽  
Salt Mine ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon ◽  
Sulfated Glycolipid ◽  
Genotypic Characteristics

A novel extremely halophilic archaeon KCY07-B2T was isolated from a salt mine in Kuche county, Xinjiang province, China. Colonies were cream-pigmented and cells were pleomorphic rod-shaped. Strain KCY07-B2T was able to grow at 25–50 °C (optimum 37–45 °C) and pH 6.0–8.0 (optimum 7.0). The strain required at least 1.9 M NaCl for growth. MgCl2 was not required. Cells lysed in distilled water. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both C20C20 and C20C25 glycerol diethers, together with five glyolipids. The bis-sulfated glycolipid S2-DGD-1 was present. The DNA G+C content was 62.5 mol%. Analysis of the 16S rRNA gene sequence revealed that strain KCY07-B2T was closely related to Halopiger xanaduensis SH-6T and Halopiger aswanensis 56T (95.8 % and 95.5 % similarity, respectively). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KCY07-B2T is considered to represent a novel species of the genus Halopiger , for which the name Halopiger salifodinae sp. nov. is proposed. The type strain is KCY07-B2T ( = JCM 18547T = CGMCC 1.12284T).

Halopenitus malekzadehii sp. nov., an extremely halophilic archaeon isolated from a salt lake

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3232-3236 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Antonio Ventosa
Keyword(s):  
Type Species ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Brine Sample ◽  
Extremely Halophilic Archaeon

Strain CC65T, a novel extremely halophilic archaeon, was isolated from a brine sample of a salt lake in Iran. The novel strain was light yellow-pigmented, non-motile, pleomorphic and required at least 1.7 M NaCl and 0.02 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.4 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, while it was able to grow over a pH and a temperature range of pH 6.5–9.0 and 30–50 °C, respectively. Analysis of 16S rRNA gene sequence revealed that strain CC65T clustered with the sole member of the genus Halopenitus , Halopenitus persicus DC30T with a sequence similarity of 98.0 %. The polar lipid profile of strain CC65T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. An unidentified glycolipid and two minor phospholipids were also observed. The only quinone present was MK-8(II-H2). The DNA G+C content of strain CC65T was 63.8 mol%. On the basis of the biochemical and physiological characteristics, as well as DNA–DNA hybridization (44 % with Halopenitus persicus IBRC 10041T), strain CC65T is classified as a novel species of the genus Halopenitus , for which the name Halopenitus malekzadehii sp. nov. is proposed. The type strain is CC65T ( = IBRC-M 10418T = KCTC 4045T).

scholarly journals Tessaracoccus lapidicaptus sp. nov., an actinobacterium isolated from the deep subsurface of the Iberian pyrite belt

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3546-3552 ◽  
Author(s):  
Fernando Puente-Sánchez ◽  
Mónica Sánchez-Román ◽  
Ricardo Amils ◽  
Víctor Parro
Keyword(s):  
Type Species ◽  
Optimal Growth ◽  
Iberian Pyrite Belt ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Optimal Value ◽  
Ph Range

A novel actinobacterium, designated IPBSL-7T, was isolated from a drilling core 297 m deep obtained from the Iberian Pyrite Belt. The strain was isolated anaerobically using nitrate as the electron acceptor. 16S rRNA gene sequence analysis revealed that it was related to Tessaracoccus flavescens SST-39T (95.7 % similarity), Tessaracoccus bendigoensis Ben 106T (95.7 %), Tessaracoccus lubricantis KSS-17SeT (95.6 %) and Tessaracoccus oleiagri SL014B-20A1T (95.0 %), while its similarity to any other member of the family Propionibacteriaceae was less than 94 %. Cells were non-motile, non-spore-forming, Gram-positive, oval to rod-shaped, and often appeared in pairs or small groups. The strain was facultatively anaerobic, oxidase-negative, catalase-positive and capable of reducing nitrate. Colonies were circular, convex, smooth and colourless. The organism could grow at between 15 and 40 °C, with an optimal growth at 37 °C. The pH range for growth was from pH 6 to 9, with pH 8 being the optimal value. Strain IPBSL-7T had peptidoglycan type A3-γ′, with ll-diaminopimelic acid as the diagnostic diamino-acid and glycine at position 1 of the peptide subunit. The dominant menaquinone was MK-9(H4) (93.8 %). The major cellular fatty acid was anteiso-C15 : 0 (55.0 %). The DNA G+C content was 70.3 mol%. On the basis of phenotypic and phylogenetic results, strain IPBSL-7T can be differentiated from previously described species of the genus Tessaracoccus and, therefore, represents a novel species, for which the name Tessaracoccus lapidicaptus sp. nov. is proposed. The type strain is IPBSL-7T ( = CECT 8385T = DSM 27266T).

Georgenia sediminis sp. nov., a moderately thermophilic actinobacterium isolated from sediment

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4243-4247 ◽  
Author(s):  
Zhi-Qing You ◽  
Jie Li ◽  
Sheng Qin ◽  
Xin-Peng Tian ◽  
Fa-Zuo Wang ◽  
...  
Keyword(s):  
Type Species ◽  
Minor Component ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Nacl Concentration ◽  
Ph Range ◽  
A Minor

A Gram-stain-positive actinobacterium, designated strain SCSIO 15020T, was isolated from sediment of the South China Sea, and characterized by using a polyphasic approach. The temperature range for growth was 24–60 °C, with optimal growth occurring at 50 °C. The pH range for growth was 6–10 (optimum pH 8–9). The NaCl concentration range for growth was 0–5 % (w/v). The peptidoglycan type was A4α. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown polar lipid. The major menaquinone was MK-8(H4); MK-7(H4) was present as a minor component. The major fatty acids (>5 %) were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. The DNA G+C content of strain SCSIO 15020T was 73.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 15020T belonged to the genus Georgenia , with the closest neighbours being Georgenia muralis 1A-CT (96.3 % similarity), Georgenia thermotolerans TT02-04T (95.7 %) and Georgenia ruanii YIM 004T (95.6 %). Based on evidence from the present polyphasic study, strain SCSIO 15020T is considered to represent a novel species of the genus Georgenia , for which the name Georgenia sediminis sp. nov. is proposed. The type strain is SCSIO 15020T ( = DSM 25884T = NBRC 108941T).

scholarly journals Halorubrum persicum sp. nov., an extremely halophilic archaeon isolated from sediment of a hypersaline lake

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1770-1778 ◽  
Author(s):  
Paulina Corral ◽  
Rafael R. de la Haba ◽  
Cristina Sánchez-Porro ◽  
Mohammad Ali Amoozegar ◽  
R. Thane Papke ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon belonging to the genus Halorubrum , strain C49T, was isolated from sediment of the hypersaline lake Aran-Bidgol in Iran. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain C49T was closely related to Halorubrum saccharovorum JCM 8865T (99.5 %) and other species of the genus Halorubrum . Studies based on multilocus sequence analysis revealed that strain C49T is placed among the species of Halorubrum ; the strain constituted a defined branch in comparison with the type strains of species of Halorubrum , while the 16S rRNA gene sequence divergence could not define the status of the newly isolated strain. For optimum growth, strain C49T required 20 % (w/v) salts at pH 7.0 and 37 °C under aerobic conditions. Mg2+ was not required. The cells were pleomorphic rods, motile and stained Gram-variable. Colonies of the strain were pink. Hypotonic treatment with <12 % NaCl provoked cell lysis. The polar lipid pattern of strain C49T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester derived from both C20C20 and C20C25 archaeol, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The DNA G+C content was 64.2 mol%. DNA–DNA hybridization studies and average nucleotide identity confirmed that strain C49T constitutes a distinct genospecies. Data obtained in this study show that strain C49T represents a novel species, for which the name Halorubrum persicum sp. nov. is proposed. The type strain is C49T ( = IBRC-M 10232T = JCM 30541T).

scholarly journals Vulcanisaeta thermophila sp. nov., a hyperthermophilic and acidophilic crenarchaeon isolated from solfataric soil

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 201-205 ◽  
Author(s):  
Kyung June Yim ◽  
In-Tae Cha ◽  
Jin-Kyu Rhee ◽  
Hye Seon Song ◽  
Dong-Wook Hyun ◽  
...  
Keyword(s):  
Type Species ◽  
Optimal Growth ◽  
The Philippines ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Mayon Volcano ◽  
Final Electron ◽  
The Republic ◽  
Ph Range

An anaerobic, rod-shaped, hyperthermophilic and acidophilic crenarchaeon, designated strain CBA1501T, was isolated from solfataric soil of the Mayon volcano in the Republic of the Philippines. Phylogenetic analysis showed that strain CBA1501T is affiliated with the genus Vulcanisaeta in the phylum Crenarchaeota . DNA sequence similarities between the 16S rRNA gene of strain CBA1501T and those of Vulcanisaeta distributa IC-017T and Vulcanisaeta souniana IC-059T were 98.5 and 97.4 %, respectively. Strain CBA1501T grew between 75–90 °C, over a pH range of 4.0–6.0 and in the presence of 0–1.0 % (w/v) NaCl, with optimal growth occurring at 85 °C, pH 5.0, and with 0 % (w/v) NaCl. Fumarate, malate, oxidized glutathione, sulfur and thiosulfate were used as final electron acceptors, but FeCl3, nitrate and sulfate were not. The DNA G+C content of strain CBA1501T was 43.1 mol%. On the basis of polyphasic taxonomic analysis, strain CBA1501T represents a novel species of the genus Vulcanisaeta in the phylum Crenarchaeota , for which we propose the name Vulcanisaeta thermophila sp. nov. The type strain is CBA1501T ( = ATCC BAA-2415T = JCM 17228T).

Halorientalis persicus sp. nov., an extremely halophilic archaeon isolated from a salt lake and emended description of the genus Halorientalis

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 940-944 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Maliheh Mehrshad ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Salt Lake ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Content Type ◽  
Link Type ◽  
Brine Sample ◽  
Emended Description ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain D108T, was isolated from a brine sample of Aran-Bidgol salt lake in Iran. The novel strain was cream-pigmented, motile, pleomorphic rod-shaped and required at least 2.5 M NaCl but not MgCl2 for growth. Optimal growth was achieved with 4.3 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, and the strain was able to grow over a pH range of 6.5 to 9.0, and a temperature range of 30 to 50 °C. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain D108T clustered with the type strain of the sole species of the genus Halorientalis , Halorientalis regularis TNN28T, with a sequence similarity of 98.8 %. The polar lipid pattern of strain D108T consisted of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, one phosphoglycolipid and two glycolipids. The only quinone present was MK-8(II-H2). The DNA G+C content of strain D108T was 62.8 mol%. DNA–DNA hybridization studies (45 % with Halorientalis regularis IBRC-M 10760T), as well as biochemical and physiological characterization, allowed strain D108T to be differentiated from Halorientalis regularis . A novel species of the genus Halorientalis , Halorientalis persicus sp. nov., is therefore proposed to accommodate this strain. The type strain is D108T ( = IBRC-M 10043T = CECT 8375T). An emended description of the genus Halorientalis is also proposed.

Litorilinea aerophila gen. nov., sp. nov., an aerobic member of the class Caldilineae , phylum Chloroflexi , isolated from an intertidal hot spring

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1149-1154 ◽  
Author(s):  
Varsha Kale ◽  
Snædís H. Björnsdóttir ◽  
Ólafur H. Friðjónsson ◽  
Sólveig K. Pétursdóttir ◽  
Sesselja Ómarsdóttir ◽  
...  
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Phenotypic Differences ◽  
Link Type ◽  
Fermentative Growth ◽  
Distinct Lineage ◽  
Ph Range

A thermophilic, aerobic, Gram-stain-negative, filamentous bacterium, strain PRI-4131T, was isolated from an intertidal hot spring in Isafjardardjup, NW Iceland. The strain grew chemo-organotrophically on various carbohydrates. The temperature range for growth was 40–65 °C (optimum 55 °C), the pH range was pH 6.5–9.0 (optimum pH 7.0) and the NaCl range was 0–3 % (w/v) (optimum 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRI-4131T represented a distinct lineage within the class Caldilineae of the phylum Chloroflexi. The highest levels of sequence similarity, about 91 %, were with Caldilinea aerophila STL-6-O1T and Caldilinea tarbellica D1-25-10-4T. Fermentative growth was not observed for strain PRI-4131T, which, in addition to other characteristics, distinguished it from the two Caldilinea species. Owing to both phylogenetic and phenotypic differences from the described members of the class Caldilineae , we propose to accommodate strain PRI-4131T in a novel species in a new genus, Litorilinea aerophila gen. nov., sp. nov. The type strain of Litorilinea aerophila is PRI-4131T ( = DSM 25763T  = ATCC BAA-2444T).

Rhizobium paknamense sp. nov., isolated from lesser duckweeds (Lemna aequinoctialis)

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3823-3828 ◽  
Author(s):  
Chokchai Kittiwongwattana ◽  
Chitti Thawai
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Sequence Similarity ◽  
Housekeeping Genes ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Low Levels ◽  
Sequence Similarity Analysis ◽  
Ph Range

A Gram-stain-negative, rod-shaped bacterium was isolated and designated strain L6-8T during a study of endophytic bacterial communities in lesser duckweed (Lemna aequinoctialis). Cells of strain L6-8T were motile with peritrichous flagella. The analysis of the nearly complete 16S rRNA gene sequence indicated that strain L6-8T was phylogenetically related to species of the genus Rhizobium . Its closest relatives were Rhizobium borbori DN316T (97.6 %), Rhizobium oryzae Alt 505T (97.3 %) and Rhizobium pseudoryzae J3-A127T (97.0 %). The sequence similarity analysis of housekeeping genes recA, glnII, atpD and gyrB showed low levels of sequence similarity (<91.5 %) between strain L6-8T and other species of the genus Rhizobium with validly published names. The pH range for growth was 4.0–9.0 (optimum 6.0–7.0), and the temperature range for growth was 20–45 °C (optimum 30 °C). Strain L6-8T tolerated NaCl up to 2 % (w/v) (optimum 1 % NaCl). The predominant components of cellular fatty acids were C19 : 0 cyclo ω8c (31.32 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 25.39 %) and C16 : 0 (12.03 %). The DNA G+C content of strain L6-8T was 60.4 mol% (T m). nodC and nifH were not amplified in strain L6-8T. DNA–DNA relatedness between strain L6-8T and R. borbori DN316T, R. oryzae Alt505T and R. pseudoryzae J3-A127T was between 11.2 and 18.3 %. Based on the sequence similarity analyses, phenotypic, biochemical and physiological characteristics and DNA–DNA hybridization, strain L6-8T could be readily distinguished from its closest relatives and represents a novel species of the genus Rhizobium , for which the name Rhizobium paknamense sp. nov. is proposed. The type strain is L6-8T ( = NBRC 109338T = BCC 55142T).

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