scholarly journals Flavobacterium gyeonganense sp. nov., isolated from freshwater, and emended descriptions of Flavobacterium chungangense, Flavobacterium aquidurense, Flavobacterium tructae and Flavobacterium granuli

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4173-4178 ◽  
Author(s):  
Haneul Kim ◽  
Heeyoung Kang ◽  
Yochan Joung ◽  
Kiseong Joh
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
High Sequence Similarity ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Distinct Lineage ◽  
Major Respiratory Quinone

A Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated HME7524T, was isolated from freshwater of Gyeong-an stream in Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME7524T formed a distinct lineage within the genus Flavobacterium and exhibited high sequence similarity with Flavobacterium chungangense CJ7T (96.9 %), Flavobacterium aquidurense WB-1.1.56T (96.9 %), Flavobacterium tructae 435-08T (96.9 %) and Flavobacterium granuli Kw05T (96.9 %). The major fatty acids of strain HME7524T were iso-C15 : 0 (26.5 %) and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 17.4 %). The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The major respiratory quinone was MK-6. The DNA G+C content of strain HME7524T was 34.2 mol%. On the basis of the evidence presented in this study, strain HME7524T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium gyeonganense sp. nov., is proposed. The type strain is HME7524T ( = KACC 17688T = CECT 8365T). Emended descriptions of Flavobacterium chungangense , Flavobacterium aquidurense , Flavobacterium tructae and Flavobacterium granuli are also provided.

scholarly journals Flavobacterium palustre sp. nov., isolated from wetland soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 1003-1007 ◽  
Author(s):  
Hao Feng ◽  
Yanhua Zeng ◽  
Yili Huang
Keyword(s):  
Type Species ◽  
Polar Lipids ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Major Respiratory Quinone ◽  
Xixi Wetland

A Gram-staining-negative, non-motile, yellow-coloured, rod-shaped bacterium, designated S44T, was isolated from bankside soil of Xixi wetland, located in Zhejiang province, China. Growth of strain S44T was observed at 6–37 °C (optimum, 28 °C) and at pH 6.0–9.0 (optimum, 7.0). No growth occurred in the presence of >2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S44T represented a member of the genus Flavobacterium , showing the highest sequence similarities to the sequences from Flavobacterium succinicans DSM 4002T (96.9 %), Flavobacterium reichenbachii WB 3.2-61T (96.6 %) and Flavobacterium glycines NCBI 105008T (96.5 %). The G+C content of the genomic DNA was 33.6 mol%. The predominant cellular fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), and the major respiratory quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids and four unknown polar lipids. On the basis of the phenotypic and genotypic data, it is proposed that the isolate S44T be classified as representing a novel species of the genus Flavobacterium , for which the name Flavobacterium palustre sp. nov. is proposed. The type strain is S44T ( = CGMCC 1.12811T = NBRC 110389T).

Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium , Flavobacterium resistens and Flavobacterium johnsoniae

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 2018-2024 ◽  
Author(s):  
Joong-Jae Kim ◽  
Eiko Kanaya ◽  
Hang-Yeon Weon ◽  
Yuichi Koga ◽  
Kazufumi Takano ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Flavobacterium Johnsoniae ◽  
Major Respiratory Quinone

A strictly aerobic, Gram-negative, yellow-pigmented, non-spore-forming rod, designated 15C3T, was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9–33 °C (optimum 25 °C) and pH 5.6–7.9 (optimum pH 6.1–7.0). No growth occurred with >2 % (w/v) NaCl. Strain 15C3T reduced nitrate to nitrogen and showed catalase activity but not oxidase activity. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The isolate contained phosphatidylethanolamine as the major polar lipid and menaquinone-6 as the major respiratory quinone. The G+C content of the genomic DNA of strain 15C3T was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15C3T belonged to the genus Flavobacterium and was most closely related to Flavobacterium hercynium WB 4.2-33T (96.9 % sequence similarity). On the basis of phenotypic and phylogenetic distinctiveness, strain 15C3T is considered to represent a novel species in the genus Flavobacterium , for which the name Flavobacterium compostarboris sp. nov. is proposed. The type strain is 15C3T ( = KACC 14224T  = JCM 16527T). Emended descriptions of F. hercynium , Flavobacterium resistens and Flavobacterium johnsoniae are also given.

scholarly journals Algoriphagus jejuensis sp. nov., isolated from seawater

2012 ◽  
Vol 62 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Dong-Heon Lee ◽  
Hyung-Yeel Kahng ◽  
Sun Bok Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
High Sequence Similarity ◽  
Respiratory Quinone ◽  
Distinct Lineage ◽  
Major Respiratory Quinone

A Gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040T, was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 5.5–10.0 and 0–5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040T belonged to a distinct lineage in the genus Algoriphagus and exhibited high sequence similarity with Algoriphagus terrigena DS-44T (98.3 %) and Algoriphagus alkaliphilus AC-74T (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus Algoriphagus. DNA–DNA relatedness between strain CNU040T and A. terrigena KCTC 12545T was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C15 : 0 (28.6 %) and summed feature 3 (consisting of iso-C15 : 0 2-OH and/or C16 : 1ω7c; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040T represents a novel species within the genus Algoriphagus, for which the name Algoriphagus jejuensis sp. nov. is proposed. The type strain is CNU040T ( = KCTC 22647T  = JCM 16112T).

scholarly journals Marinobacter changyiensis, sp. nov., isolated from offshore sediment

2020 ◽  
Vol 70 (5) ◽  
pp. 3004-3011 ◽  
Author(s):  
Guangyu Li ◽  
Shanshan Wang ◽  
Yingbao Gai ◽  
Xiupian Liu ◽  
Qiliang Lai ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Genomic Analysis ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Promising Alternative ◽  
Content Type ◽  
Link Type ◽  
Marine Sediment Sample ◽  
Major Respiratory Quinone

An aerobic, Gram-stain-negative bacterium, designated CLL7-20T, was isolated from a marine sediment sample from offshore of Changyi, Shandong Province, China. Cells of strain CLL7-20T were rod-shaped, motile with one or more polar flagella, and grew optimally at pH 7.0, at 28 °C and with 3 % (w/v) NaCl. The principal fatty acids of strain CLL7-20T were C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c). The main polar lipids of strain CLL7-20T were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and an unidentified aminolipid (AL). Strain CLL7-20T contained Q-9 as the major respiratory quinone. The G+C content of its genomic DNA was 56.2 mol%. Phylogenetically, strain CLL7-20T branched within the genus Marinobacter , with M. daqiaonensis YCSA40T being its closest phylogenetic relative (96.7 % 16S rRNA gene sequence similarity), followed by M. sediminum R65T (96.6 %). Average nucleotide identity and in silico DNA–DNA hybridization values between strain CLL7-20T and the closest related reference strains were 73.2% and 19.8 %, respectively. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, we suggest that strain CLL7-20T (=MCCC 1A14855T=KCTC 72664T) is the type strain of a novel species in the genus Marinobacter , for which the name Marinobacter changyiensis sp. nov. is proposed. Based on the genomic analysis, siderophore genes were found from strain CLL7-20T, which indicate its potential as a promising alternative to chemical fertilizers in iron-limitated environments such as saline soils.

scholarly journals Roseivivax marinus sp. nov., isolated from deep water

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2540-2544 ◽  
Author(s):  
Xiaofeng Dai ◽  
Xiaochong Shi ◽  
Xin Gao ◽  
Jingli Liu ◽  
Xiao-Hua Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Deep Water ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone

A Gram-stain-negative, strictly aerobic, non-motile, cream, long rod-shaped bacterium, designated strain ZL136T, was isolated from deep water of the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain belonged to the genus Roseivivax with highest sequence similarity to Roseivivax halodurans OCh 239T (97.0 %), followed by Roseivivax isoporae sw-2T (96.9 %). Growth occurred at 4–37 °C (optimum 32 °C), pH 6.0–10.0 (optimum 8.0) and in the presence of 0–12 % (w/v) NaCl (optimum 3–4 %) in marine broth 2216. Strain ZL136T did not produce bacteriochlorophyll a. The predominant fatty acids were C18 : 1ω7c and/or C18 : 1ω6c, C18 : 0, C16 : 0 and 11-methyl C18 : 1ω7c. The major polar lipids of ZL136T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified lipid. The major respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content of strain ZL136T was 67.0 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain ZL136T was classified as a representative of a novel species in the genus of Roseivivax , for which the name Roseivivax marinus sp. nov. is proposed. The type strain is ZL136T ( = JCM 19386T = KCTC 32470T).

Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 21-26 ◽  
Author(s):  
Haneul Kim ◽  
Yochan Joung ◽  
Kiseong Joh
Keyword(s):  
Tidal Flat ◽  
Type Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Emended Description

A Gram-staining-negative, aerobic and rod-shaped bacterial strain, designated HMC4223T, was isolated from a tidal flat in Taean, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMC4223T formed a lineage within the genus Algoriphagus and exhibited highest sequence similarity to Algoriphagus hitonicola 7-UAHT (96.3 %). The major fatty acids of strain HMC4223T were iso-C15 : 0 and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c). The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine and five unidentified polar lipids. The DNA G+C content of strain HMC4223T was 48.4 mol%. On the basis of the evidence presented in this study, strain HMC4223T represents a novel species of the genus Algoriphagus , for which the name Algoriphagus taeanensis sp. nov. is proposed, with the type strain HMC4223T ( = KCTC 22709T = NBRC 105728T). An emended description of Algoriphagus hitonicola is also presented.

scholarly journals Mucilaginibacter aquaedulcis sp. nov., isolated from fresh water

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 698-703 ◽  
Author(s):  
Yochan Joung ◽  
Heeyoung Kang ◽  
Beom-Il Lee ◽  
Haneul Kim ◽  
Kiseong Joh ◽  
...  
Keyword(s):  
Fresh Water ◽  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone ◽  
The Republic

An aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain PGW1-R01T, was isolated from fresh water from the Yeongju in the Republic of Korea. The strain grew optimally at 30 °C and at pH 6-8 on R2A agar. The major cellular fatty acids were summed feature 3 [comprising C16 : 1ω7c and/or C16 : 1ω6c (50.2 %) and iso-C15 : 0 (24.8 %)]. The major respiratory quinone was MK-7. The G+C contents were 39.4 mol% and the predominant respiratory quinone was MK-7. Based on 16S rRNA gene phylogeny, the strain belongs to the genus Mucilaginibacter . The strain PGW1-R01T was closely related to ‘ Mucilaginibacter ginsenosidivorax’ (96.6 % sequence similarity), Mucilaginibacter lappiensis (96.4 %) and Mucilaginibacter flavus (96.4 %). On the basis of the evidence presented in this study, strain PGW1-R01T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter http://dx.doi.org/10.1601/nm.11437 aquaedulcis sp. nov., is proposed. The type strain is PGW1-R01T( = KCTC 23942T = CECT 8102T).

Sediminibacterium ginsengisoli sp. nov., isolated from soil of a ginseng field, and emended descriptions of the genus Sediminibacterium and of Sediminibacterium salmoneum

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 905-912 ◽  
Author(s):  
Yeon-Ju Kim ◽  
Ngoc-Lan Nguyen ◽  
Hang-Yeon Weon ◽  
Deok-Chun Yang
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-negative bacterium, designated DCY13T, was isolated from soil of a ginseng field in South Korea. Comparative analysis of 16S rRNA gene sequences showed that strain DCY13T shared the highest sequence similarity (95.0 %) with Sediminibacterium salmoneum NBRC 103935T and 87.6–91.4 % sequence similarity with other members of the family Chitinophagaceae . Cells were non-spore-forming rods, catalase- and oxidase-positive, motile by gliding and facultatively anaerobic. The only respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G. The G+C content of the genomic DNA was 47.5±1.0 mol%. In addition to phosphatidylethanolamine, the major polar lipids were two unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The major cell-wall sugars were ribose, xylose and galactose. It is proposed that strain DCY13T represents a novel species in the genus Sediminibacterium , for which the name Sediminibacterium ginsengisoli sp. nov. is proposed. The type strain is DCY13T ( = KCTC 12833T  = JCM 15794T  = DSM 22335T). Emended descriptions of the genus Sediminibacterium and of Sediminibacterium salmoneum are also proposed.

Kordia antarctica sp. nov., isolated from Antarctic seawater

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3617-3622 ◽  
Author(s):  
Kiwoon Baek ◽  
Ahyoung Choi ◽  
Ilnam Kang ◽  
Kiyoung Lee ◽  
Jang-Cheon Cho
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
The Antarctic

A Gram-staining-negative, chemoheterotrophic, yellow-pigmented, non-motile, flexirubin-negative, facultatively anaerobic bacterium, designated strain IMCC3317T, was isolated from a coastal seawater sample from the Antarctic Penninsula. Optimal growth of strain IMCC3317T was observed at 20 °C, pH 8.0 and in the presence of 2–3 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC3317T belonged to the genus Kordia and was closely related to Kordia algicida OT-1T (96.7 % sequence similarity) and Kordia periserrulae IMCC1412T (96.1 % sequence similarity). The major fatty acids were 10-methyl C16 : 0 and/or iso-C16 : 1ω9c, iso-C17 : 0 3-OH, iso-C15 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 35.1 mol%. The strain contained menaquinone-6 (MK-6) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine and unknown aminophospholipids, aminolipids and polar lipids. On the basis of phylogenetic distinction and differential phenotypic characteristics, it is suggested that strain IMCC3317T ( = KCTC 32292T = NBRC 109401T) be assigned to the genus Kordia as the type strain of a novel species, for which the name Kordia antarctica sp. nov. is proposed.

scholarly journals Flavobacterium suzhouense sp. nov., isolated from farmland river sludge

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 370-374 ◽  
Author(s):  
Hao Zhang ◽  
Ming-gen Cheng ◽  
Bin Sun ◽  
Su-hui Guo ◽  
Man Song ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone

A Gram-stain-negative bacterium, designated XIN-1T, was isolated from a farmland river sludge sample in Suzhou, China. Cells of strain XIN-1T were strictly aerobic, non-motile and rod-shaped. Strain XIN-1T grew optimally at pH 7.0 and 28 °C. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain XIN-1T was most closely related to Flavobacterium hauense BX12T (98.2 % sequence similarity), followed by Flavobacterium beibuense F44-8T (96.3 %). The major respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. The major fatty acids (>5 %) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH. The genomic DNA G+C content of strain XIN-1T was 39.8 mol%. Strain XIN-1T showed low DNA–DNA relatedness with F. hauense BX12T (38.7±0.5 %). On the basis of genotypic and phenotypic data, strain XIN-1T is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium suzhouense sp. nov. is proposed. The type strain is XIN-1T ( = CCTCC AB 2014200T = KCTC 42107T).

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