scholarly journals Pseudoalteromonas spongiae sp. nov., a novel member of the γ-Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters

2005 ◽  
Vol 55 (4) ◽  
pp. 1593-1596 ◽  
Author(s):  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Sergey Dobretsov ◽  
Yulia Plakhotnikova ◽  
...  
Keyword(s):  
Hong Kong ◽  
Gene Sequence ◽  
Novel Species ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Hong Kong Waters ◽  
The 16S Rrna Gene

A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006T) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006T did not have flagella and were non-motile. Colonies were pale orange in colour, 2–4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006T was heterotrophic, strictly aerobic and required NaCl for growth (2·0–6·0 %). It grew at pH 5·0–10·0 and between 12 and 44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006T within the genus Pseudoalteromonas of the γ-subclass of the Proteobacteria. The DNA G+C content is 40·6 mol% and the dominant fatty acids were 12 : 0 3-OH, 14 : 0, 15 : 0 iso 2-OH, 16 : 0, 16 : 1ω7, 17 : 1ω8 and 18 : 1ω7 (altogether representing 75·9 % of the total).These data supported the affiliation of UST010723-006T to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95·4 to 96·8 %. UST010723-006T differed from these closest relatives by 9–19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006T constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006T (=NRRL B-41100T=JCM 12884T).

scholarly journals Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core

2007 ◽  
Vol 57 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Yoon-Gon Kim ◽  
Dong Han Choi ◽  
Sangmin Hyun ◽  
Byung Cheol Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sediment Core ◽  
Gene Sequence ◽  
Novel Species ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
The 16S Rrna Gene

A halotolerant, facultatively alkaliphilic bacterium, designated CL-MP28T, was isolated from the surface of a sediment core sample collected at a depth of 2247 m in the Ulleung Basin of the East Sea, Korea. Phylogenetic analysis of the 16S rRNA gene sequence of strain CL-MP28T revealed an affiliation with the genus Oceanobacillus. The sequence similarities between the isolate and type strains of members of the genus Oceanobacillus were in the range 95.0–96.0 %, indicating that strain CL-MP28T represented a novel species within the genus. The strain was found to be Gram-positive, rod-shaped and motile by means of peritrichous flagella and was shown to produce ellipsoidal spores. The strain was strictly aerobic and able to grow with NaCl at concentrations in the range 0–14 % (w/v) at pH 7.5. The strain grew at temperatures of 15–42 °C and at pH 6.5–9.5. The major fatty acids were anteiso-C15 : 0 (64.9 %), anteiso-C17 : 0 (11.9 %) and iso-C16 : 0 (7.7 %). The major isoprenoid quinone was MK-7. The DNA G+C content was 40.2 mol%. According to the 16S rRNA gene sequence, DNA–DNA relatedness and physiological data and the fatty acid composition, CL-MP28T could be assigned to the genus Oceanobacillus, but is distinguishable from the recognized species of this genus. Strain CL-MP28T therefore represents a novel species within the genus Oceanobacillus, for which the name Oceanobacillus profundus sp. nov. is proposed. The type strain is CL-MP28T (=KCCM 42318T=DSM 18246T).

Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 869-873 ◽  
Author(s):  
Bokun Lin ◽  
Guoyong Lu ◽  
Yandan Zheng ◽  
Wei Xie ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Red Alga ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
The 16S Rrna Gene

A novel yellow-pigmented, agarolytic bacterial strain, designated ZC1T, was isolated from the surface of the marine red alga Porphyra haitanensis collected near Nan Ao Island, Guangdong province, China. The isolate was Gram-stain-negative, strictly aerobic and rod-shaped and displayed β-galactosidase, alkaline phosphatase, catalase and oxidase activities. The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH. The major menaquinone was menaquinone 6 (MK-6). The DNA G+C content was 32.8 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain ZC1T was closely related to members of the genus Aquimarina in the family Flavobacteriaceae , phylum Bacteroidetes . Based on phylogenetic and phenotypic evidence, strain ZC1T ( = CCTCC AB 2010229T  = NBRC 107695T) represents the type strain of a novel species in the genus Aquimarina , for which the name Aquimarina agarilytica sp. nov. is proposed.

scholarly journals Mucilaginibacter ginsengisoli sp. nov., isolated from a ginseng-cultivated soil

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3933-3937 ◽  
Author(s):  
Jae-Hyung Ahn ◽  
Byoung-chan Kim ◽  
Jae-Ho Joa ◽  
Soo-Jin Kim ◽  
Jaekyeong Song ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Optimum Ph ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Dark Pink

A dark-pink-coloured bacterial strain, B4Y-8T, was isolated from a soil cultivated with ginseng. The 16S rRNA gene sequence of this strain showed highest similarity with Mucilaginibacter litoreus BR-18T (96.8 %), Mucilaginibacter lutimaris BR-3T (96.6 %) and Mucilaginibacter defluvii A5T (96.2 %) among the type strains of species of the genus Mucilaginibacter. Strain B4Y-8T was a strictly aerobic, Gram-stain-negative, non-motile, short-rod-shaped bacterium producing a large amount of extracellular polymeric substance. The strain grew at 10–35 °C (optimum, 25 °C), at pH 3.0–11.0 (optimum, pH 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). The DNA G+C content of strain B4Y-8T was 49.0 mol%. It contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C15 : 0 as the major fatty acids. On the basis of evidence from the present polyphasic taxonomic study, strain B4Y-8T should be classified as representing a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter ginsengisoli sp. nov. is proposed. The type strain is B4Y-8T ( = KACC 18152T = JCM 30759T).

scholarly journals Actinoalloteichus hymeniacidonis sp. nov., an actinomycete isolated from the marine sponge Hymeniacidon perleve

2006 ◽  
Vol 56 (10) ◽  
pp. 2309-2312 ◽  
Author(s):  
Haitao Zhang ◽  
Wen Zheng ◽  
Jianyu Huang ◽  
Hongli Luo ◽  
Yan Jin ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Gene Sequence ◽  
Novel Species ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Actinomycete Strain ◽  
Type Strains ◽  
The 16S Rrna Gene

A polyphasic study was undertaken to establish the taxonomic status of a Gram-positive, aerobic actinomycete, strain HPA177T, isolated from a marine sponge, Hymeniacidon perleve. The organism formed branching, non-fragmenting vegetative hyphae and produced black pigment. Chemotaxonomic characteristics were consistent with its assignment to the genus Actinoalloteichus. Analysis of the 16S rRNA gene sequence showed that strain HPA177T formed a robust clade with type strains of the genus Actinoalloteichus, but was distinct from them. A number of phenotypic characteristics also readily distinguished strain HPA177T from species of the genus Actinoalloteichus with validly published names. On the basis of the above data, it is proposed that strain HPA177T represents a novel species, Actinoalloteichus hymeniacidonis sp. nov. The type strain of Actinoalloteichus hymeniacidonis is HPA177T (=CGMCC 4.2500T=JCM 13436T).

scholarly journals Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas

2005 ◽  
Vol 55 (4) ◽  
pp. 1589-1592 ◽  
Author(s):  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
Sergey Dobretsov ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Gliding Motility ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Characteristics ◽  
The Bahamas ◽  
The Family ◽  
Winogradskyella Poriferorum ◽  
The 16S Rrna Gene

A Gram-negative, rod-shaped bacterium (designated strain UST030701-295T) with fast gliding motility was isolated from the surface of the sponge Lissodendoryx isodictyalis in the Bahamas. Colonies of UST030701-295T were yellow in colour, 2–4 mm in diameter, convex with a smooth surface and entire margins. UST030701-295T was heterotrophic, strictly aerobic and required NaCl for growth (1·0–4·0 %). Growth was observed at pH 6·0–10·0 and at 12–44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST030701-295T within the genus Winogradskyella of the family Flavobacteriaceae, sharing 94·7–95·8 % similarity with the three recognized members of the genus. The G+C content of the DNA was 32·8 mol% and the predominant fatty acids were iso-C15 : 1, iso-C15 : 0, iso-C15 : 0 2-OH, iso-C15 : 0 3-OH, iso-C16 : 0 3-OH, C16 : 1 ω7 and iso-C17 : 0 3-OH (together representing 75·4 % of the total); these data supported the affiliation of UST030701-295T to the genus Winogradskyella. UST030701-295T differed from the three recognized species of Winogradskyella in 7–17 traits. Molecular evidence together with phenotypic characteristics suggests that UST030701-295T represents a novel species within the genus Winogradskyella, for which the name Winogradskyella poriferorum sp. nov. is proposed. The type strain is UST030701-295T (=NRRL B-41101T=JCM 12885T).

scholarly journals Streptomyces sparsus sp. nov., isolated from a saline and alkaline soil

2011 ◽  
Vol 61 (7) ◽  
pp. 1601-1605 ◽  
Author(s):  
Yi Jiang ◽  
Yan-Ru Cao ◽  
Jutta Wiese ◽  
Shu-Kun Tang ◽  
Li-Hua Xu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Alkaline Soil ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Aerial Hyphae ◽  
Actinomycete Strain ◽  
The 16S Rrna Gene

A salt- and alkali-tolerant actinomycete strain, YIM 90018T, was isolated from a saline and alkaline soil sample collected from Qinghai, China. Aerial hyphae of strain YIM 90018T were only produced on YIM 82 agar. Vegetative hyphae were well developed and did not fragment. Straight or flexuous (rectiflexibiles) spore chains were produced. The isolate grew well with 25 % (w/v) MgCl2 . 6H2O and at pH 10. All of these characters indicated that strain YIM 90018T belonged to the genus Streptomyces. On the basis of phylogenetic analysis of the 16S rRNA gene sequence, DNA–DNA hybridization and phenotypic characteristics, strain YIM 90018T could be differentiated from all recognized species of the genus Streptomyces. A novel species, Streptomyces sparsus sp. nov., is proposed, with strain YIM 90018T ( = CCTCC AA204019T = DSM 41858T) as the type strain.

Rhodanobacter soli sp. nov., isolated from soil of a ginseng field

2010 ◽  
Vol 60 (12) ◽  
pp. 2935-2939 ◽  
Author(s):  
Thi Phuong Nam Bui ◽  
Yeon-Ju Kim ◽  
Hobin Kim ◽  
Deok-Chun Yang
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Respiratory Quinone ◽  
Yellow Pigments ◽  
Type Strains ◽  
The 16S Rrna Gene

Strain DCY45T was isolated from soil of a ginseng field in Pocheon Province, Korea. Strain DCY45T was Gram-negative, oxidase- and catalase-positive, motile and rod-shaped and produced yellow pigments on R2A agar. The organism grew optimally at 30 °C and at pH 7.0. The G+C content of the genomic DNA was 65.4 mol%. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C17 : 1 ω9c, iso-C16 : 0 and iso-C15 : 0. Phylogenetic analysis based on the 16S rRNA gene sequence was used to determine the taxonomic position of strain DCY45T, which is most closely related to species of the genus Rhodanobacter, with similarity levels of 96.0–98.4 %; DNA–DNA relatedness with related strains was lower than 60 %. Strain DCY45T differed significantly from related type strains in phenotypic characteristics. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY45T represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter soli sp. nov. is proposed. The type strain is DCY45T (=KCTC 22620T =JCM 16126T).

scholarly journals Caldithrix palaeochoryensis sp. nov., a thermophilic, anaerobic, chemo-organotrophic bacterium from a geothermally heated sediment, and emended description of the genus Caldithrix

2010 ◽  
Vol 60 (9) ◽  
pp. 2120-2123 ◽  
Author(s):  
Margarita L. Miroshnichenko ◽  
Tatyana V. Kolganova ◽  
Stefan Spring ◽  
Nikolay Chernyh ◽  
Elizaveta A. Bonch-Osmolovskaya
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Single Cells ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Rrna Gene Sequence ◽  
Phenotypic Characteristics ◽  
Emended Description ◽  
The 16S Rrna Gene

A novel thermophilic, strictly anaerobic, chemo-organotrophic bacterium, designated MCT, was isolated from a geothermally heated sediment of a marine hydrothermal system at Palaeochory Bay, Milos, Greece. Cells of strain MCT were rods of variable length (4–12 μm) and width (0.2–0.3 μm), occurring as single cells or forming large aggregates that were visible as flocs. Strain MCT grew optimally at pH 7.0 and 60 °C and with 3 % (w/v) NaCl. Strain MCT grew chemo-organoheterotrophically and fermented peptides and di- and polysaccharides in the presence of 0.1 g yeast extract l−1. The DNA G+C content of strain MCT was 43.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain MCT within the genus Caldithrix. However, strain MCT possessed certain phenotypic features that differentiated it from the type strain of the only species of the genus Caldithrix described to date. On the basis of phylogenetic and phenotypic characteristics, it is proposed that strain MCT represents a novel species, Caldithrix palaeochoryensis sp. nov. The type strain is MCT (=DSM 21940T =VKM B-2536T). In addition, an emended description of the genus Caldithrix is presented.

scholarly journals Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters

2004 ◽  
Vol 54 (6) ◽  
pp. 2281-2284 ◽  
Author(s):  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
Madeline Wu ◽  
...  
Keyword(s):  
Hong Kong ◽  
Fatty Acid ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Nearest Neighbours ◽  
Hong Kong Waters ◽  
The 16S Rrna Gene

A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009PT) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009PT and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65·9–66·2 mol%) and fatty acid (86·22–88·52 % 18 : 1ω7c) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009PT within the Rhodobacter group of the α-subclass of the Proteobacteria. The nearest neighbours belong to the genus Loktanella, with similarity values ranging from 94·5 to 95·5 %. Data on G+C and fatty acid contents support the affiliation to the genus Loktanella. UST950701-009PT and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2·0–14·0 %). Both grow in pH 5·0–10·0 and at 8–44 °C. Both are positive in oxidase, catalase and β-galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009PT constitutes a novel species within the genus Loktanella. The name Loktanella hongkongensis sp. nov. is proposed; the type strain is UST950701-009PT (=NRRL B-41039T=JCM 12479T) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).

scholarly journals Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora

2004 ◽  
Vol 54 (6) ◽  
pp. 2053-2056 ◽  
Author(s):  
Tomohiko Tamura ◽  
Takeshi Sakane
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Subtropical Forest ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Monophyletic Cluster ◽  
Aerial Hyphae ◽  
Emended Description ◽  
The 16S Rrna Gene

An actinomycete that developed sporangia containing four spores in a single row at the ends of short sporangiophores on branched aerial hyphae was isolated from subtropical forest soil. The isolate contained menaquinone MK-9(H4), glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids and madurose in the whole-cell hydrolysate. The 16S rRNA gene sequence indicated that the isolate formed a monophyletic cluster with Planotetraspora mira. On the basis of morphological and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species of the genus Planotetraspora is proposed, Planotetraspora silvatica sp. nov. (type strain, TT 00-51T=NBRC 100141T=DSM 44746T).

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