Pedobacter aquatilis sp. nov., isolated from drinking water, and emended description of the genus Pedobacter

A Gram-negative, rod-shaped, non-spore-forming bacterium (strain AR107T) was isolated from the drinking water distribution system of Seville (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus Pedobacter. On the basis of 16S rRNA gene sequence comparisons, strain AR107T was shown to belong to the phylum Bacteroidetes, being related to members of the genus Pedobacter. It showed 95.2 % sequence similarity with respect to the type strains of Pedobacter heparinus and Pedobacter piscium, and 94.1 % similarity with respect to the type strain of Pedobacter himalayensis. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), which supports the affiliation of strain AR107T to the genus Pedobacter. The DNA G+C content of this strain was 38 mol%. On the basis of the phenotypic, phylogenetic and genotypic results, strain AR107T represents a novel species, for which the name Pedobacter aquatilis sp. nov. is proposed. The type strain is AR107T (=CCM 7347T=CECT 7114T=JCM 13454T).

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Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64264-0 ◽

2006 ◽

Vol 56 (7) ◽

pp. 1589-1592 ◽

Cited By ~ 32

Author(s):

Virginia Gallego ◽

Maria Teresa García ◽

Antonio Ventosa

Keyword(s):

Drinking Water ◽

Distribution System ◽

Water Distribution ◽

Bacterial Population ◽

Water Distribution System ◽

Sequence Similarity ◽

Rrna Gene ◽

Drinking Water Distribution ◽

Type Strains ◽

The 16S Rrna Gene

Strain VP48T was isolated from drinking water during a screening programme to monitor the bacterial population present in the water distribution system of Sevilla (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus Chryseobacterium, members of which are widely distributed in soil, water and clinical sources. However, the 16S rRNA gene sequence similarity values of strain VP48T to the type strains of Chryseobacterium species were 96 % or lower. Furthermore, phenotypic characteristics clearly indicated that the isolate represents a novel Chryseobacterium species, for which the name Chryseobacterium hispanicum sp. nov. is proposed; strain VP48T (=CECT 7129T=CCM 7359T=JCM 13554T) is the type strain. The DNA G+C content of this strain is 34.3 mol%.

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Roseomonas aquatica sp. nov., isolated from drinking water

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64379-0 ◽

2006 ◽

Vol 56 (10) ◽

pp. 2291-2295 ◽

Cited By ~ 49

Author(s):

Virginia Gallego ◽

Cristina Sánchez-Porro ◽

Maria Teresa García ◽

Antonio Ventosa

Keyword(s):

Drinking Water ◽

16S Rrna ◽

16S Rrna Gene ◽

Distribution System ◽

Water Distribution ◽

Sequence Data ◽

Sequence Similarity ◽

Rrna Gene ◽

Strictly Aerobic ◽

Drinking Water Distribution

Strain TR53T, a Gram-negative, non-motile, non-spore-forming and strictly aerobic coccobacillus, isolated from the drinking water distribution system of Seville, Spain, was subjected to polyphasic taxonomic analysis using a combination of phenotypic, genotypic and phylogenetic features. Phylogenetic analysis of 16S rRNA gene sequences showed that strain TR53T had highest similarity to members of the genus Roseomonas, with sequence similarity values between 95.7 % (to Roseomonas genomospecies 5 strain ATCC 49960) and 94.0 % (to Roseomonas gilardii subsp. rosea ATCC 49956T). On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (68.6 mol%), strain TR53T represents a novel species of the genus Roseomonas, for which the name Roseomonas aquatica sp. nov. is proposed. The type strain of Roseomonas aquatica is TR53T (=CECT 7131T=JCM 13556T).

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Massilia aurea sp. nov., isolated from drinking water

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64389-0 ◽

2006 ◽

Vol 56 (10) ◽

pp. 2449-2453 ◽

Cited By ~ 60

Author(s):

Virginia Gallego ◽

Cristina Sánchez-Porro ◽

Maria Teresa García ◽

Antonio Ventosa

Keyword(s):

Drinking Water ◽

Distribution System ◽

Water Distribution ◽

Rrna Gene ◽

Phenotypic Properties ◽

Sequence Comparisons ◽

Hybridization Data ◽

A Value ◽

Drinking Water Distribution ◽

Taxonomic Approach

A Gram-negative, motile, rod-shaped organism, strain AP13T, able to produce yellow-pigmented colonies, was isolated from the drinking water distribution system of Seville (Spain) and was characterized by using a polyphasic taxonomic approach. In 16S rRNA gene sequence comparisons, strain AP13T exhibited 96.9–95.6 % similarity with respect to the five recognized species of the genus Massilia. The DNA G+C content of strain AP13T was 66.0 mol%, a value that supports the affiliation of strain AP13T to the genus Massilia. DNA–DNA hybridization data and phenotypic properties confirmed that strain AP13T represents a novel species of the genus Massilia, for which the name Massilia aurea sp. nov. is proposed. The type strain is AP13T (=CECT 7142T=CCM 7363T=DSM 18055T=JCM 13879T).

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Microbial Community Profile of a Lead Service Line Removed from a Drinking Water Distribution System

Applied and Environmental Microbiology ◽

10.1128/aem.02446-10 ◽

2011 ◽

Vol 77 (15) ◽

pp. 5557-5561 ◽

Cited By ~ 21

Author(s):

Colin White ◽

Matthew Tancos ◽

Darren A. Lytle

Keyword(s):

Drinking Water ◽

Microbial Community ◽

Distribution System ◽

Water Distribution ◽

Water Distribution System ◽

Rrna Gene ◽

Drinking Water Distribution System ◽

Service Line ◽

Drinking Water Distribution ◽

Microbial Community Profile

ABSTRACTA corroded lead service line was removed from a drinking water distribution system, and the microbial community was profiled using 16S rRNA gene techniques. This is the first report of the characterization of a biofilm on the surface of a corroded lead drinking water service line. The majority of phylotypes have been linked to heavy-metal-contaminated environments.

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Ammonia- and Nitrite-Oxidizing Bacterial Communities in a Pilot-Scale Chloraminated Drinking Water Distribution System

Applied and Environmental Microbiology ◽

10.1128/aem.68.1.73-81.2002 ◽

2002 ◽

Vol 68 (1) ◽

pp. 73-81 ◽

Cited By ~ 163

Author(s):

John M. Regan ◽

Gregory W. Harrington ◽

Daniel R. Noguera

Keyword(s):

Drinking Water ◽

Distribution System ◽

Distribution Systems ◽

Water Distribution ◽

Rflp Analysis ◽

Drinking Water Treatment ◽

Pilot Scale ◽

Rrna Gene ◽

Ammonia Oxidizing Bacteria ◽

Drinking Water Distribution

ABSTRACT Nitrification in drinking water distribution systems is a common operational problem for many utilities that use chloramines for secondary disinfection. The diversity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the distribution systems of a pilot-scale chloraminated drinking water treatment system was characterized using terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene (ribosomal DNA [rDNA]) cloning and sequencing. For ammonia oxidizers, 16S rDNA-targeted T-RFLP indicated the presence of Nitrosomonas in each of the distribution systems, with a considerably smaller peak attributable to Nitrosospira-like AOB. Sequences of AOB amplification products aligned within the Nitrosomonas oligotropha cluster and were closely related to N. oligotropha and Nitrosomonas ureae. The nitrite-oxidizing communities were comprised primarily of Nitrospira, although Nitrobacter was detected in some samples. These results suggest a possible selection of AOB related to N. oligotropha and N. ureae in chloraminated systems and demonstrate the presence of NOB, indicating a biological mechanism for nitrite loss that contributes to a reduction in nitrite-associated chloramine decay.

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Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63966-0 ◽

2006 ◽

Vol 56 (2) ◽

pp. 339-342 ◽

Cited By ~ 39

Author(s):

Virginia Gallego ◽

Maria Teresa García ◽

Antonio Ventosa

Keyword(s):

Drinking Water ◽

Distribution System ◽

Water Distribution ◽

Sequence Data ◽

Methylotrophic Bacterium ◽

Rrna Gene ◽

Strictly Aerobic ◽

Solid Media ◽

Drinking Water Distribution ◽

Agar Surface

A pink-pigmented, facultatively methylotrophic strain, AR27T, isolated from the drinking water distribution system of Seville, Spain, was characterized taxonomically. Cells were Gram-negative rods, motile, non-spore-forming and strictly aerobic. Growth in liquid media was flocculant whereas on solid media growth produced colonies that usually adhered to the agar surface. On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (63·6 mol%), strain AR27T (=CECT 7069T=CCM 7305T) is proposed as the type strain of a novel species of the genus Methylobacterium, Methylobacterium adhaesivum sp. nov.

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Bacterial composition in a metropolitan drinking water distribution system utilizing different source waters

Journal of Water and Health ◽

10.2166/wh.2014.057 ◽

2014 ◽

Vol 13 (1) ◽

pp. 140-151 ◽

Cited By ~ 18

Author(s):

Vicente Gomez-Alvarez ◽

Ben W. Humrighouse ◽

Randy P. Revetta ◽

Jorge W. Santo Domingo

Keyword(s):

Drinking Water ◽

Community Structure ◽

Distribution System ◽

Water Distribution ◽

Water Distribution System ◽

Rrna Gene ◽

Drinking Water Distribution System ◽

Bacterial Composition ◽

Service Areas ◽

Drinking Water Distribution

We investigated the bacterial composition of water samples from two service areas within a drinking water distribution system (DWDS), each associated with a different primary source of water (groundwater, GW; surface water, SW) and different treatment process. Community analysis based on 16S rRNA gene clone libraries indicated that Actinobacteria (Mycobacterium spp.) and α-Proteobacteria represented nearly 43 and 38% of the total sequences, respectively. Sequences closely related to Legionella, Pseudomonas, and Vibrio spp. were also identified. In spite of the high number of sequences (71%) shared in both areas, multivariable analysis revealed significant differences between the GW and SW areas. While the dominant phylotypes where not significantly contributing in the ordination of samples, the populations associated with the core of phylotypes (1–10% in each sample) significantly contributed to the differences between both service areas. Diversity indices indicate that the microbial community inhabiting the SW area is more diverse and contains more distantly related species coexisting with local assemblages as compared with the GW area. The bacterial community structure of SW and GW service areas were dissimilar, suggesting that their respective source water and/or water quality parameters shaped by the treatment processes may contribute to the differences in community structure observed.

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Assessment of Lisbon drinking water distribution network biofilm colonization and associated hazards

Water Science & Technology Water Supply ◽

10.2166/ws.2008.103 ◽

2008 ◽

Vol 8 (4) ◽

pp. 421-426

Author(s):

J. Menaia ◽

M. Benoliel ◽

A. Lopes ◽

C. Neto ◽

E. Ferreira ◽

...

Keyword(s):

Drinking Water ◽

Distribution System ◽

Water Distribution ◽

Water Distribution System ◽

Water Distribution Network ◽

Storage Tanks ◽

Water Pipe ◽

Cultural Methods ◽

Drinking Water Distribution ◽

And Storage

Concerns arise from the possible occurrence of pathogens in drinking water pipe biofilms and storage tank sediments. In these studies, biofilm samples from pipes and sediments from storage tanks of the Lisbon drinking water distribution system were analyzed. Protein determinations and heterotrophic counts on pipe biofilm samples were used to assess the Lisbon network sessile colonization intensity and distribution. Indicator and pathogenic microorganisms were analyzed in pipe biofilm samples, as well as in storage tanks biofilm and sediments, by using cultural methods and PCR, to assess risks. Results have shown that the Lisbon network sessile colonization is relatively weak in intensity. In addition, no meaningful hazards were apparent for both the network biofilm and the storage tanks biofilm and sediments.

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