scholarly journals Dermacoccus barathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetes isolated from deep-sea mud of the Mariana Trench

2006 ◽  
Vol 56 (10) ◽  
pp. 2303-2307 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
Alan T. Bull ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line ◽  
Sea Mud ◽  
Type Strains

The taxonomic positions of two actinobacterial strains isolated from Mariana Trench sediment were established using a combination of genotypic and phenotypic data. The strains, isolates MT2.1T and MT2.2T, formed a distinct phyletic line in the Micrococcineae 16S rRNA gene tree together with Dermacoccus abyssi NCIMB 14084T. The isolates had chemical and phenotypic properties typical of members of the genus Dermacoccus and could be distinguished sharply from one another and from the type strains of Dermacoccus abyssi and Dermacoccus nishinomiyaensis using DNA–DNA relatedness data. A range of phenotypic properties served to distinguish the two novel strains from one another and from the type strains of established Dermacoccus species. The G+C contents of the DNAs of strains MT2.1T and MT2.2T were 66.8 and 69.1 mol%, respectively. It is evident that the two isolates merit recognition as novel species within the genus Dermacoccus. The names proposed for these taxa are Dermacoccus barathri sp. nov. (type strain MT2.1T=DSM 17574T=NCIMB 14081T) and Dermacoccus profundi sp. nov. (type strain MT2.2T=DSM 17575T=NCIMB 14084T).

scholarly journals Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line

The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA–DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.

scholarly journals Dermacoccus abyssi sp. nov., a piezotolerant actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (6) ◽  
pp. 1233-1237 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Dna Relatedness ◽  
Taxonomic Approach

The taxonomic status of an actinobacterial strain isolated from Mariana Trench sediment was determined using a polyphasic taxonomic approach. The strain, isolate MT1.1T, formed a distinct clade in the Micrococcineae 16S rRNA gene tree together with Dermacoccus nishinomiyaensis DSM 20448T. The organism had chemical and phenotypic properties consistent with its classification in the genus Dermacoccus and could be distinguished from D. nishinomiyaensis DSM 20448T using DNA–DNA relatedness and phenotypic data. The G+C content of the DNA of isolate MT1.1T was 65.2 mol%. It is evident that the organism merits recognition as a novel species in the genus Dermacoccus. The name proposed for this taxon is Dermacoccus abyssi sp. nov.; the type strain is MT1.1T (=DSM 17573T=NCIMB 14084T). The organism grows well at 40 MPa and hence is piezotolerant.

scholarly journals Streptomyces atacamensis sp. nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Chile

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2680-2684 ◽  
Author(s):  
Rakesh Santhanam ◽  
Chinyere K. Okoro ◽  
Xiaoying Rong ◽  
Ying Huang ◽  
Alan T. Bull ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Tree ◽  
Atacama Desert ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Content Type ◽  
Arid Soil ◽  
Link Type ◽  
Phyletic Line

The taxonomic position of a Streptomyces strain isolated from an extreme hyper-arid soil sample collected from the Atacama Desert was determined using a polyphasic approach. The strain, isolate C60T, had chemical and morphological features typical of members of the genus Streptomyces and formed a distinct phyletic line in the Streptomyces 16S rRNA gene tree, together with the type strain of Streptomyces radiopugnans . The two strains were distinguished readily using a combination of phenotypic properties and by a DNA–DNA relatedness value of 23.17 (±0.95) %. On the basis of these genotypic and phenotypic data, it is proposed that isolate C60T ( = CGMCC 4.7018T = KACC 15492T) be classified in the genus Streptomyces as Streptomyces atacamensis sp. nov.

scholarly journals Nocardia jiangxiensis sp. nov. and Nocardia miyunensis sp. nov., isolated from acidic soils

2005 ◽  
Vol 55 (5) ◽  
pp. 1921-1925 ◽  
Author(s):  
Qingfeng Cui ◽  
Liming Wang ◽  
Ying Huang ◽  
Zhiheng Liu ◽  
Michael Goodfellow
Keyword(s):  
Novel Species ◽  
Gene Tree ◽  
Rrna Gene ◽  
Acidic Soils ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Soil Actinomycetes ◽  
Distinct Branch ◽  
Type Strains

The taxonomic positions of two soil actinomycetes, strains 117T and 43401T, provisionally assigned to the genus Nocardia were determined in a polyphasic study. The organisms were found to have phenotypic properties typical of members of the genus Nocardia, and formed a distinct branch in the Nocardia 16S rRNA gene tree. It was evident from the phylogenetic data that the isolates were most closely, albeit loosely, associated with the type strains of Nocardia nova and Nocardia pseudobrasiliensis. However, all four of these organisms were readily distinguishable from one another using DNA–DNA relatedness and phenotypic data. It is evident from the genotypic and phenotypic data that the two isolates should be recognized as novel species of the genus Nocardia. It is proposed, therefore, that strains 117T (=CGMCC 4.1904T=JCM 12860T) and 43401T (=CGMCC 4.1905T=JCM 12861T) be classified in the genus Nocardia as the type strains of Nocardia miyunensis sp. nov. and Nocardia jiangxiensis sp. nov., respectively.

scholarly journals Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.

2010 ◽  
Vol 60 (4) ◽  
pp. 769-775 ◽  
Author(s):  
Yashawant Kumar ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Streptomyces Hygroscopicus ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data

A polyphasic study was undertaken to determine the taxonomic status of six strains received as Streptomyces hygroscopicus. The strains had chemotaxonomic and morphological properties typical of members of the genus Streptomyces and formed distinct phyletic lines in the Streptomyces 16S rRNA gene tree. These strains were distinguished from one another and from phylogenetically close neighbours using a combination of phenotypic properties. The combined genotypic and phenotypic data showed that all six strains form distinct centres of taxonomic variation within the genus Streptomyces. The following novel species are proposed to accommodate the strains: Streptomyces aldersoniae sp. nov. (type strain DSM 41909T =NRRL 18513T), Streptomyces angustmyceticus sp. nov., comb. nov. (type strain DSM 41683T=NRRL B-2347T), Streptomyces ascomycinicus sp. nov. (type strain DSM 40822T =NBRC 13981T), Streptomyces decoyicus sp. nov., comb. nov. (type strain DSM 41427T =NRRL 2666T), Streptomyces milbemycinicus sp. nov. (type strain DSM 41911T =NRRL 5739T) and Streptomyces wellingtoniae sp. nov. (type strain DSM 40632T =NRRL B-1503T).

Lechevalieria atacamensis sp. nov., Lechevalieria deserti sp. nov. and Lechevalieria roselyniae sp. nov., isolated from hyperarid soils

2010 ◽  
Vol 60 (2) ◽  
pp. 296-300 ◽  
Author(s):  
Chinyere K. Okoro ◽  
Alan T. Bull ◽  
Ankur Mutreja ◽  
Xiaoying Rong ◽  
Ying Huang ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Atacama Desert ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Type Strains ◽  
Relatedness Data

The taxonomic positions of three Lechevalieria-like strains isolated from hyperarid soils of the Atacama Desert, Chile, were established by using a polyphasic approach. The organisms had chemical and morphological properties consistent with their classification in the genus Lechevalieria. They formed a distinct subclade in the Lechevalieria 16S rRNA gene clade and were most closely related to the type strain of Lechevalieria xinjiangensis. DNA–DNA relatedness data showed that each of the isolates and Lechevalieria xinjiangensis DSM 45081T belong to distinct genomic species. The new isolates and the type strains of recognized Lechevalieria species were readily distinguished based on a number of phenotypic properties. A combination of the genotypic and phenotypic data showed that the three isolates represent three novel species of the genus Lechevalieria. The names proposed for these taxa are Lechevalieria atacamensis sp. nov. (type strain C61T =CGMCC 4.5536T =NRRL B-24706T), Lechevalieria deserti sp. nov. (type strain C68T =CGMCC 4.5535T =NRRL B-24707T) and Lechevalieria roselyniae sp. nov. (type strain C81T =CGMCC 4.5537T =NRRL B-24708T).

scholarly journals Isoptericola chiayiensis sp. nov., isolated from mangrove soil

2011 ◽  
Vol 61 (7) ◽  
pp. 1667-1670 ◽  
Author(s):  
M. Tseng ◽  
H. C. Liao ◽  
W. P. Chiang ◽  
G. F. Yuan
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Mangrove Soil ◽  
Low Levels ◽  
Type Strains

A novel actinomycete, designated strain 06182M-1T, was isolated from a mangrove soil sample collected from Chiayi County in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.8 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain 06182M-1T within the genus Isoptericola. However, the low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain 06182M-1T represents a novel species of the genus Isoptericola, for which the name Isoptericola chiayiensis sp. nov. is proposed. The type strain is 06182M-1T ( = BCRC 16888T  = KCTC 19740T).

scholarly journals Corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer

2010 ◽  
Vol 60 (5) ◽  
pp. 1135-1140 ◽  
Author(s):  
Jia Tong ◽  
Chengxu Liu ◽  
Paula H. Summanen ◽  
Huaxi Xu ◽  
Sydney M. Finegold
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Corynebacterium Glucuronolyticum ◽  
Taxonomic Methods ◽  
Groin Abscess

A coryneform strain, 06-1773OT (=WAL 19168T), derived from a groin abscess sample was characterized using phenotypic and molecular taxonomic methods. Comparative analyses revealed more than 3 % divergence of the 16S rRNA gene sequence and about 10 % divergence of the partial rpoB gene sequence from the type strain of Corynebacterium glucuronolyticum. The strain could also be differentiated from C. glucuronolyticum by a set of phenotypic properties. A DNA–DNA relatedness study between strain WAL 19168T and C. glucuronolyticum CCUG 35055T showed a relatedness value of 13.3 % (13.7 % on repeat analysis). The genotypic and phenotypic data show that the strain merits classification within a novel species of Corynebacterium. We propose the name Corynebacterium pyruviciproducens sp. nov. for the novel species. The type strain is 06-1773OT (=WAL 19168T =CCUG 57046T =ATCC BAA-1742T).

scholarly journals Rhodococcus gordoniae sp. nov., an actinomycete isolated from clinical material and phenol-contaminated soil

2004 ◽  
Vol 54 (2) ◽  
pp. 407-411 ◽  
Author(s):  
Amanda L. Jones ◽  
June M. Brown ◽  
Vachaspati Mishra ◽  
John D. Perry ◽  
Arnold G. Steigerwalt ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Tree ◽  
Growth Cycle ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Gene Similarity

The taxonomic relationships of two actinomycetes provisionally assigned to the genus Rhodococcus were determined using a polyphasic taxonomic approach. The generic assignment was confirmed by 16S rRNA gene similarity data, as the organisms, strains MTCC 1534 and W 4937T, were shown to belong to the Rhodococcus rhodochrous subclade. These organisms had phenotypic properties typical of rhodococci; they were aerobic, Gram-positive, weakly acid-fast actinomycetes that showed an elementary branching-rod–coccus growth cycle and contained meso-diaminopimelic acid, arabinose and galactose in whole-organism hydrolysates, N-glycolated muramic acid residues, dehydrogenated menaquinones with eight isoprene units as the predominant isoprenologue and mycolic acids that co-migrated with those extracted from the type strain of R. rhodochrous. The strains had identical phenotypic profiles and belong to the same genomic species, albeit one distinguished from Rhodococcus pyridinivorans, with which they formed a distinct phyletic line. They were also distinguished from representatives of all of the species classified in the R. rhodochrous 16S rRNA gene tree using a set of phenotypic features. The genotypic and phenotypic data show that the strains merit recognition as a novel species of Rhodococcus. The name proposed is Rhodococcus gordoniae sp. nov., with the type strain W 4937T (=DSM 44689T=NCTC 13296T).

scholarly journals Amycolatopsis plumensis sp. nov., a novel bioactive actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil

2005 ◽  
Vol 55 (5) ◽  
pp. 2057-2061 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Hamid Amir ◽  
René Pineau ◽  
G. Y. Annie Tan ◽  
Michael Goodfellow
Keyword(s):  
Gene Sequence ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Properties ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Ultramafic Soil ◽  
Type Strains

The taxonomic position of an actinomycete isolated from a brown hypermagnesian ultramafic soil was examined using a polyphasic approach. The organism, which was designated SBHS Strp1T, was found to have chemical and morphological properties typical of Amycolatopsis strains. It was most closely associated with Amycolatopsis kentuckyensis, Amycolatopsis lexingtonensis, Amycolatopsis rifamycinica, Amycolatopsis pretoriensis and Amycolatopsis tolypomycina on the basis of 16S rRNA gene sequence data, and showed a unique pattern of phenotypic properties that distinguished it from the type strains of these taxa. The combined genotypic and phenotypic data show that the organism merits description as a novel species of Amycolatopsis. The name proposed for the novel species is Amycolatopsis plumensis sp. nov.; the type strain is SBHS Strp1T (=DSM 44776T=NRRL B-24324T).

Sign in / Sign up

Export Citation Format

Share Document