scholarly journals Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line

The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA–DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.

scholarly journals Dermacoccus barathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetes isolated from deep-sea mud of the Mariana Trench

2006 ◽  
Vol 56 (10) ◽  
pp. 2303-2307 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
Alan T. Bull ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line ◽  
Sea Mud ◽  
Type Strains

The taxonomic positions of two actinobacterial strains isolated from Mariana Trench sediment were established using a combination of genotypic and phenotypic data. The strains, isolates MT2.1T and MT2.2T, formed a distinct phyletic line in the Micrococcineae 16S rRNA gene tree together with Dermacoccus abyssi NCIMB 14084T. The isolates had chemical and phenotypic properties typical of members of the genus Dermacoccus and could be distinguished sharply from one another and from the type strains of Dermacoccus abyssi and Dermacoccus nishinomiyaensis using DNA–DNA relatedness data. A range of phenotypic properties served to distinguish the two novel strains from one another and from the type strains of established Dermacoccus species. The G+C contents of the DNAs of strains MT2.1T and MT2.2T were 66.8 and 69.1 mol%, respectively. It is evident that the two isolates merit recognition as novel species within the genus Dermacoccus. The names proposed for these taxa are Dermacoccus barathri sp. nov. (type strain MT2.1T=DSM 17574T=NCIMB 14081T) and Dermacoccus profundi sp. nov. (type strain MT2.2T=DSM 17575T=NCIMB 14084T).

scholarly journals Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.

2010 ◽  
Vol 60 (4) ◽  
pp. 769-775 ◽  
Author(s):  
Yashawant Kumar ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Streptomyces Hygroscopicus ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data

A polyphasic study was undertaken to determine the taxonomic status of six strains received as Streptomyces hygroscopicus. The strains had chemotaxonomic and morphological properties typical of members of the genus Streptomyces and formed distinct phyletic lines in the Streptomyces 16S rRNA gene tree. These strains were distinguished from one another and from phylogenetically close neighbours using a combination of phenotypic properties. The combined genotypic and phenotypic data showed that all six strains form distinct centres of taxonomic variation within the genus Streptomyces. The following novel species are proposed to accommodate the strains: Streptomyces aldersoniae sp. nov. (type strain DSM 41909T =NRRL 18513T), Streptomyces angustmyceticus sp. nov., comb. nov. (type strain DSM 41683T=NRRL B-2347T), Streptomyces ascomycinicus sp. nov. (type strain DSM 40822T =NBRC 13981T), Streptomyces decoyicus sp. nov., comb. nov. (type strain DSM 41427T =NRRL 2666T), Streptomyces milbemycinicus sp. nov. (type strain DSM 41911T =NRRL 5739T) and Streptomyces wellingtoniae sp. nov. (type strain DSM 40632T =NRRL B-1503T).

scholarly journals Dermacoccus abyssi sp. nov., a piezotolerant actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (6) ◽  
pp. 1233-1237 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Dna Relatedness ◽  
Taxonomic Approach

The taxonomic status of an actinobacterial strain isolated from Mariana Trench sediment was determined using a polyphasic taxonomic approach. The strain, isolate MT1.1T, formed a distinct clade in the Micrococcineae 16S rRNA gene tree together with Dermacoccus nishinomiyaensis DSM 20448T. The organism had chemical and phenotypic properties consistent with its classification in the genus Dermacoccus and could be distinguished from D. nishinomiyaensis DSM 20448T using DNA–DNA relatedness and phenotypic data. The G+C content of the DNA of isolate MT1.1T was 65.2 mol%. It is evident that the organism merits recognition as a novel species in the genus Dermacoccus. The name proposed for this taxon is Dermacoccus abyssi sp. nov.; the type strain is MT1.1T (=DSM 17573T=NCIMB 14084T). The organism grows well at 40 MPa and hence is piezotolerant.

scholarly journals Actinomadura sputi sp. nov., isolated from the sputum of a patient with pulmonary infection

2010 ◽  
Vol 60 (1) ◽  
pp. 149-153 ◽  
Author(s):  
A. F. Yassin ◽  
C. Spröer ◽  
C. Siering ◽  
H.-P. Klenk
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Gene Tree ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Phyletic Line ◽  
Actinomycete Strain ◽  
Taxonomic Approach ◽  
The 16S Rrna Gene

The taxonomic position of an actinomycete, strain IMMIB L-889T, isolated from the sputum of a 64-year-old man, was determined using a polyphasic taxonomic approach. The strain had chemical and morphological properties that were consistent with its classification in the genus Actinomadura. It formed a distinct phyletic line in the 16S rRNA gene tree of Actinomadura and was most closely related to the type strain of Actinomadura hallensis (98.4 % sequence similarity), but could be readily distinguished from the latter species using DNA–DNA relatedness and phenotypic data. The combined genotypic and phenotypic data indicate that strain IMMIB L-889T represents a novel species of the genus Actinomadura, for which the name Actinomadura sputi sp. nov. is proposed. The type strain is IMMIB L-889T (=DSM 45233T=CCUG 56587T).

scholarly journals Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses

2007 ◽  
Vol 57 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Nagamani Bora ◽  
Marc Vancanneyt ◽  
Roberto Gelsomino ◽  
Jean Swings ◽  
Noelle Brennan ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Protein Patterns ◽  
Phenotypic Data ◽  
The Subject ◽  
Distinct Branch ◽  
Sequence Similarities

Seven Gram-positive, coryneform bacteria with virtually identical whole-organism protein patterns were isolated from the surface of smear-ripened cheeses. Representatives of these strains were the subject of a polyphasic study designed to establish their taxonomic status. The organisms formed a distinct branch in the Microbacteriaceae 16S rRNA gene tree and were most closely related to members of the genus Agrococcus, sharing sequence similarities of 95.4–98.7 %. The chemotaxonomic profiles of the strains were consistent with their classification in the genus Agrococcus. The combined genotypic and phenotypic data show that the isolates should be classified in the genus Agrococcus as representatives of a novel species. The name Agrococcus casei sp. nov. is proposed for this taxon. Isolate R-17892t2T (=DSM 18061T=LMG 22410T) is the type strain of Agrococcus casei sp. nov.

scholarly journals Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov.

2006 ◽  
Vol 56 (5) ◽  
pp. 1109-1115 ◽  
Author(s):  
Chunguang Xu ◽  
Liming Wang ◽  
Qingfeng Cui ◽  
Ying Huang ◽  
Zhiheng Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Acidic Soils ◽  
Phenotypic Data ◽  
Streptomyces Species

The taxonomic status of six neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province, China, was established using a polyphasic approach. The morphological and chemotaxonomic characteristics revealed that the isolates belong to the genus Streptomyces. Almost complete 16S rRNA gene sequences of the isolates were determined and aligned with available corresponding sequences of representatives of the family Streptomycetaceae; phylogenetic trees were inferred using four tree-making algorithms. The isolates formed a distinct, albeit heterogeneous, subclade in the Streptomyces 16S rRNA gene tree together with the type strain of Streptomyces yeochonensis, but were readily distinguishable from the latter using DNA–DNA hybridization and phenotypic data. It was evident from the genotypic and phenotypic data that the isolates belonged to four novel Streptomyces species, for which the following names are proposed: Streptomyces guanduensis sp. nov. (type strain 701T=CGMCC 4.2022T=JCM 13274T), Streptomyces paucisporeus sp. nov. (type strain 1413T=CGMCC 4.2025T=JCM 13276T), Streptomyces rubidus sp. nov. (type strain 13c15T=CGMCC 4.2026T=JCM 13277T) and Streptomyces yanglinensis sp. nov. (type strain 1307T=CGMCC 4.2023T=JCM 13275T); isolates 317 and 913 belong to this latter species.

scholarly journals Lechevalieria xinjiangensis sp. nov., a novel actinomycete isolated from radiation-polluted soil in China

2007 ◽  
Vol 57 (12) ◽  
pp. 2819-2822 ◽  
Author(s):  
Wei Wang ◽  
Zhidong Zhang ◽  
Qiyong Tang ◽  
Jun Mao ◽  
Dong Wei ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polluted Soil ◽  
Western China ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Phyletic Line ◽  
North Western

A novel actinomycete was isolated from radiation-polluted soil collected from Xinjiang in north-western China. The isolate, strain R24T, was found to have chemical and morphological properties associated with members of the genus Lechevalieria. An almost-complete 16S rRNA gene sequence of the isolate was aligned with corresponding sequences from representatives of the genus Lechevalieria and related taxa, using three tree-making algorithms, and was shown to form a distinct phyletic line in the Lechevalieria phylogenetic tree. DNA–DNA relatedness and phenotypic data served to distinguish strain R24T from members of the three Lechevalieria species with validly published names. The genotypic and phenotypic data show that the isolate should be classified as a novel species within the genus Lechevalieria. The name proposed for this taxon is Lechevalieria xinjiangensis sp. nov. The type strain is R24T (=CGMCC 4.3525T=DSM 45081T).

scholarly journals Streptomyces atacamensis sp. nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Chile

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2680-2684 ◽  
Author(s):  
Rakesh Santhanam ◽  
Chinyere K. Okoro ◽  
Xiaoying Rong ◽  
Ying Huang ◽  
Alan T. Bull ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Tree ◽  
Atacama Desert ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Content Type ◽  
Arid Soil ◽  
Link Type ◽  
Phyletic Line

The taxonomic position of a Streptomyces strain isolated from an extreme hyper-arid soil sample collected from the Atacama Desert was determined using a polyphasic approach. The strain, isolate C60T, had chemical and morphological features typical of members of the genus Streptomyces and formed a distinct phyletic line in the Streptomyces 16S rRNA gene tree, together with the type strain of Streptomyces radiopugnans . The two strains were distinguished readily using a combination of phenotypic properties and by a DNA–DNA relatedness value of 23.17 (±0.95) %. On the basis of these genotypic and phenotypic data, it is proposed that isolate C60T ( = CGMCC 4.7018T = KACC 15492T) be classified in the genus Streptomyces as Streptomyces atacamensis sp. nov.

scholarly journals Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius

2007 ◽  
Vol 57 (12) ◽  
pp. 2799-2804 ◽  
Author(s):  
Martha E. Trujillo ◽  
Reiner M. Kroppenstedt ◽  
Carmen Fernández-Molinero ◽  
Peter Schumann ◽  
Eustoquio Martínez-Molina
Keyword(s):  
Type Strain ◽  
Sequence Data ◽  
Novel Species ◽  
Root Nodules ◽  
Taxonomic Status ◽  
Lupinus Angustifolius ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Data ◽  
Fatty Acid Patterns

A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora. According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis, Micromonospora matsumotoense and Micromonospora purpureochromogenes. Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA–DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14NT =DSM 44874T =LMG 24055T) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09T =DSM 44871T =LMG 24056T) are proposed.

scholarly journals Streptomyces burgazadensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4043-4048 ◽  
Author(s):  
Salih Saricaoglu ◽  
Kamil Isik ◽  
Aysel Veyisoglu ◽  
Hayrettin Saygin ◽  
Demet Cetin ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Tree ◽  
Diaminopimelic Acid ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Marmara Sea ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Phyletic Line

A novel actinobacterial strain, designated Z1R7T, was isolated from a soil sample collected from Burgazada, in the Marmara Sea (Turkey), and the strain identity was determined using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and it formed a distinct phyletic line in the 16S rRNA gene tree, together with the type strains Streptomyces specialis GW41-1564T (95.76 %), Streptomyces mayteni YIM 60475T (95.64 %), Streptomyces hainanensis YIM 47672T (95.53 %), Streptomyces hoynatensis S1412T (95.29 %), Streptomyces avicenniae MCCC 1A01535T (94.74 %), Streptomyces sedi YIM 65188T (94.59 %) and Streptomyces zhaozhouensis NEAU-LZS-5T (94.68 %). Chomotaxonomic data revealed that strain Z1R7T possesed MK-9 (H8) as the predominant menaquinone, ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and ribose as whole cell sugars. Diphosphatidylglycerol, phoshphatidylethanolamine and phosphatidylinositol were the predominant polar lipids; iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0 were the major fatty acids, and the genomic DNA G+C content was 69.4 mol%. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1R7T ( = KCTC 29434T = DSM 42126T) should be classified in the genus Streptomyces as Streptomyces burgazadensis sp. nov.

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