scholarly journals Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.

2010 ◽  
Vol 60 (4) ◽  
pp. 769-775 ◽  
Author(s):  
Yashawant Kumar ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Streptomyces Hygroscopicus ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data

A polyphasic study was undertaken to determine the taxonomic status of six strains received as Streptomyces hygroscopicus. The strains had chemotaxonomic and morphological properties typical of members of the genus Streptomyces and formed distinct phyletic lines in the Streptomyces 16S rRNA gene tree. These strains were distinguished from one another and from phylogenetically close neighbours using a combination of phenotypic properties. The combined genotypic and phenotypic data showed that all six strains form distinct centres of taxonomic variation within the genus Streptomyces. The following novel species are proposed to accommodate the strains: Streptomyces aldersoniae sp. nov. (type strain DSM 41909T =NRRL 18513T), Streptomyces angustmyceticus sp. nov., comb. nov. (type strain DSM 41683T=NRRL B-2347T), Streptomyces ascomycinicus sp. nov. (type strain DSM 40822T =NBRC 13981T), Streptomyces decoyicus sp. nov., comb. nov. (type strain DSM 41427T =NRRL 2666T), Streptomyces milbemycinicus sp. nov. (type strain DSM 41911T =NRRL 5739T) and Streptomyces wellingtoniae sp. nov. (type strain DSM 40632T =NRRL B-1503T).

scholarly journals Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line

The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA–DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.

scholarly journals Dermacoccus abyssi sp. nov., a piezotolerant actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (6) ◽  
pp. 1233-1237 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Dna Relatedness ◽  
Taxonomic Approach

The taxonomic status of an actinobacterial strain isolated from Mariana Trench sediment was determined using a polyphasic taxonomic approach. The strain, isolate MT1.1T, formed a distinct clade in the Micrococcineae 16S rRNA gene tree together with Dermacoccus nishinomiyaensis DSM 20448T. The organism had chemical and phenotypic properties consistent with its classification in the genus Dermacoccus and could be distinguished from D. nishinomiyaensis DSM 20448T using DNA–DNA relatedness and phenotypic data. The G+C content of the DNA of isolate MT1.1T was 65.2 mol%. It is evident that the organism merits recognition as a novel species in the genus Dermacoccus. The name proposed for this taxon is Dermacoccus abyssi sp. nov.; the type strain is MT1.1T (=DSM 17573T=NCIMB 14084T). The organism grows well at 40 MPa and hence is piezotolerant.

scholarly journals Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov.

2006 ◽  
Vol 56 (5) ◽  
pp. 1109-1115 ◽  
Author(s):  
Chunguang Xu ◽  
Liming Wang ◽  
Qingfeng Cui ◽  
Ying Huang ◽  
Zhiheng Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Phylogenetic Trees ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Acidic Soils ◽  
Phenotypic Data ◽  
Streptomyces Species

The taxonomic status of six neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province, China, was established using a polyphasic approach. The morphological and chemotaxonomic characteristics revealed that the isolates belong to the genus Streptomyces. Almost complete 16S rRNA gene sequences of the isolates were determined and aligned with available corresponding sequences of representatives of the family Streptomycetaceae; phylogenetic trees were inferred using four tree-making algorithms. The isolates formed a distinct, albeit heterogeneous, subclade in the Streptomyces 16S rRNA gene tree together with the type strain of Streptomyces yeochonensis, but were readily distinguishable from the latter using DNA–DNA hybridization and phenotypic data. It was evident from the genotypic and phenotypic data that the isolates belonged to four novel Streptomyces species, for which the following names are proposed: Streptomyces guanduensis sp. nov. (type strain 701T=CGMCC 4.2022T=JCM 13274T), Streptomyces paucisporeus sp. nov. (type strain 1413T=CGMCC 4.2025T=JCM 13276T), Streptomyces rubidus sp. nov. (type strain 13c15T=CGMCC 4.2026T=JCM 13277T) and Streptomyces yanglinensis sp. nov. (type strain 1307T=CGMCC 4.2023T=JCM 13275T); isolates 317 and 913 belong to this latter species.

scholarly journals Rhodococcus gordoniae sp. nov., an actinomycete isolated from clinical material and phenol-contaminated soil

2004 ◽  
Vol 54 (2) ◽  
pp. 407-411 ◽  
Author(s):  
Amanda L. Jones ◽  
June M. Brown ◽  
Vachaspati Mishra ◽  
John D. Perry ◽  
Arnold G. Steigerwalt ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Tree ◽  
Growth Cycle ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Gene Similarity

The taxonomic relationships of two actinomycetes provisionally assigned to the genus Rhodococcus were determined using a polyphasic taxonomic approach. The generic assignment was confirmed by 16S rRNA gene similarity data, as the organisms, strains MTCC 1534 and W 4937T, were shown to belong to the Rhodococcus rhodochrous subclade. These organisms had phenotypic properties typical of rhodococci; they were aerobic, Gram-positive, weakly acid-fast actinomycetes that showed an elementary branching-rod–coccus growth cycle and contained meso-diaminopimelic acid, arabinose and galactose in whole-organism hydrolysates, N-glycolated muramic acid residues, dehydrogenated menaquinones with eight isoprene units as the predominant isoprenologue and mycolic acids that co-migrated with those extracted from the type strain of R. rhodochrous. The strains had identical phenotypic profiles and belong to the same genomic species, albeit one distinguished from Rhodococcus pyridinivorans, with which they formed a distinct phyletic line. They were also distinguished from representatives of all of the species classified in the R. rhodochrous 16S rRNA gene tree using a set of phenotypic features. The genotypic and phenotypic data show that the strains merit recognition as a novel species of Rhodococcus. The name proposed is Rhodococcus gordoniae sp. nov., with the type strain W 4937T (=DSM 44689T=NCTC 13296T).

scholarly journals Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil

2010 ◽  
Vol 60 (8) ◽  
pp. 1813-1823 ◽  
Author(s):  
Byung-Yong Kim ◽  
James E. M. Stach ◽  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Morphological Properties ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
Type Strains

Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequence analysis showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from other Dactylosporangium type strains and from ‘Dactylosporangium salmoneum’ NRRL B-16294. Twelve of 13 representative isolates had identical 16S rRNA gene sequences and formed a subclade that was distinct from corresponding phyletic lines composed of the remaining isolate, strain BK63T, the ‘D. salmoneum’ strain and the type strains of recognized Dactylosporangium species. DNA–DNA relatedness data indicated that representatives of the multi-membered 16S rRNA gene subclade, isolate BK63T and the ‘D. salmoneum’ subclade formed distinct genomic species; all of these organisms had chemotaxonomic and morphological properties consistent with their classification in the genus Dactylosporangium. They were also distinguished from one another and from the type strains of recognized Dactylosporangium species based on a range of phenotypic properties. Combined genotypic and phenotypic data showed that isolate BK63T, isolates BK51T, BK53 and BK69, and strain NRRL B-16294T should be classified in the genus Dactylosporangium as representing novel species. The names proposed for these species are Dactylosporangium luridum sp. nov. (type strain BK63T  = DSM 45324T  = KACC 20933T  = NRRL B-24775T), Dactylosporangium luteum sp. nov. (type strain BK51T  = DSM 45323T  = KACC 20899T  = NRRL B-24774T) and Dactylosporangium salmoneum sp. nov., nom. rev. (type strain NRRL B-16294T  = ATCC 31222T  = DSM 43910T  = JCM 3272T  = NBRC 14103T).

scholarly journals Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China

2004 ◽  
Vol 54 (6) ◽  
pp. 2085-2089 ◽  
Author(s):  
Ying Huang ◽  
Wei Li ◽  
Liming Wang ◽  
Benjamin Lanoot ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Sharp Separation ◽  
Rdna Its ◽  
Phyletic Line

A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14T, was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable γ-region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus Streptomyces. The sharp separation of the organism from representatives of the genus Streptomyces was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized Streptomyces species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14T (=AS 4.1858T=JCM 12278T=LMG 22082T) should be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces glauciniger sp. nov. is proposed.

scholarly journals Dermacoccus barathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetes isolated from deep-sea mud of the Mariana Trench

2006 ◽  
Vol 56 (10) ◽  
pp. 2303-2307 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
Alan T. Bull ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line ◽  
Sea Mud ◽  
Type Strains

The taxonomic positions of two actinobacterial strains isolated from Mariana Trench sediment were established using a combination of genotypic and phenotypic data. The strains, isolates MT2.1T and MT2.2T, formed a distinct phyletic line in the Micrococcineae 16S rRNA gene tree together with Dermacoccus abyssi NCIMB 14084T. The isolates had chemical and phenotypic properties typical of members of the genus Dermacoccus and could be distinguished sharply from one another and from the type strains of Dermacoccus abyssi and Dermacoccus nishinomiyaensis using DNA–DNA relatedness data. A range of phenotypic properties served to distinguish the two novel strains from one another and from the type strains of established Dermacoccus species. The G+C contents of the DNAs of strains MT2.1T and MT2.2T were 66.8 and 69.1 mol%, respectively. It is evident that the two isolates merit recognition as novel species within the genus Dermacoccus. The names proposed for these taxa are Dermacoccus barathri sp. nov. (type strain MT2.1T=DSM 17574T=NCIMB 14081T) and Dermacoccus profundi sp. nov. (type strain MT2.2T=DSM 17575T=NCIMB 14084T).

scholarly journals Actinoplanes liguriensis sp. nov. and Actinoplanes teichomyceticus sp. nov.

2006 ◽  
Vol 56 (9) ◽  
pp. 2125-2130 ◽  
Author(s):  
Joachim M. Wink ◽  
Reiner M. Kroppenstedt ◽  
Peter Schumann ◽  
Gerhard Seibert ◽  
Erko Stackebrandt
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Actinoplanes Teichomyceticus ◽  
Physiological Properties ◽  
Phylogenetic Lineages ◽  
The 16S Rrna Gene

The taxonomic status of ‘Actinoplanes liguriae’ A/6353 and ‘Actinoplanes teichomyceticus’ AB8327 was established by using a polyphasic approach. Strains A/6353 and AB8327 form distinct phylogenetic lineages in the 16S rRNA gene tree of members of the genus Actinoplanes and are related moderately and closely to Actinoplanes rectilineatus and Actinoplanes cyaneus, respectively. Morphological, cultural and physiological properties indicated that strains A/6353 and AB8327 represent separate, novel species of the genus Actinoplanes, Actinoplanes liguriensis sp. nov. (type strain A/6353T=FH 2244T=DSM 43865T=ATCC 31048T=BCRC 12121T=CBS 355.75T=IMSNU 22127T=JCM 3250T=KCTC 9536T=KCC A-0250T=NBRC 13997T=NCIMB 12636T=NRRL B-16723T=SANK 62178T) and Actinoplanes teichomyceticus sp. nov. (type strain AB8327T=FH 2149T=DSM 43866T=ATCC 31121T=BCRC 12106T=FERM P-3462T=IMSNU 20043T=IMET 9254T=JCM 3252T=KCC A-0252T=KCTC 9543T=NBRC 13999T=NCIMB 12640T=NRRL B-16726T=SANK 60479T).

scholarly journals Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses

2007 ◽  
Vol 57 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Nagamani Bora ◽  
Marc Vancanneyt ◽  
Roberto Gelsomino ◽  
Jean Swings ◽  
Noelle Brennan ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Rrna Gene ◽  
Protein Patterns ◽  
Phenotypic Data ◽  
The Subject ◽  
Distinct Branch ◽  
Sequence Similarities

Seven Gram-positive, coryneform bacteria with virtually identical whole-organism protein patterns were isolated from the surface of smear-ripened cheeses. Representatives of these strains were the subject of a polyphasic study designed to establish their taxonomic status. The organisms formed a distinct branch in the Microbacteriaceae 16S rRNA gene tree and were most closely related to members of the genus Agrococcus, sharing sequence similarities of 95.4–98.7 %. The chemotaxonomic profiles of the strains were consistent with their classification in the genus Agrococcus. The combined genotypic and phenotypic data show that the isolates should be classified in the genus Agrococcus as representatives of a novel species. The name Agrococcus casei sp. nov. is proposed for this taxon. Isolate R-17892t2T (=DSM 18061T=LMG 22410T) is the type strain of Agrococcus casei sp. nov.

Lechevalieria atacamensis sp. nov., Lechevalieria deserti sp. nov. and Lechevalieria roselyniae sp. nov., isolated from hyperarid soils

2010 ◽  
Vol 60 (2) ◽  
pp. 296-300 ◽  
Author(s):  
Chinyere K. Okoro ◽  
Alan T. Bull ◽  
Ankur Mutreja ◽  
Xiaoying Rong ◽  
Ying Huang ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Atacama Desert ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Type Strains ◽  
Relatedness Data

The taxonomic positions of three Lechevalieria-like strains isolated from hyperarid soils of the Atacama Desert, Chile, were established by using a polyphasic approach. The organisms had chemical and morphological properties consistent with their classification in the genus Lechevalieria. They formed a distinct subclade in the Lechevalieria 16S rRNA gene clade and were most closely related to the type strain of Lechevalieria xinjiangensis. DNA–DNA relatedness data showed that each of the isolates and Lechevalieria xinjiangensis DSM 45081T belong to distinct genomic species. The new isolates and the type strains of recognized Lechevalieria species were readily distinguished based on a number of phenotypic properties. A combination of the genotypic and phenotypic data showed that the three isolates represent three novel species of the genus Lechevalieria. The names proposed for these taxa are Lechevalieria atacamensis sp. nov. (type strain C61T =CGMCC 4.5536T =NRRL B-24706T), Lechevalieria deserti sp. nov. (type strain C68T =CGMCC 4.5535T =NRRL B-24707T) and Lechevalieria roselyniae sp. nov. (type strain C81T =CGMCC 4.5537T =NRRL B-24708T).

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