scholarly journals Cryptococcus mujuensis sp. nov. and Cryptococcus cuniculi sp. nov., basidiomycetous yeasts isolated from wild rabbit faeces

2006 ◽  
Vol 56 (9) ◽  
pp. 2241-2244 ◽  
Author(s):  
Kee-Sun Shin ◽  
Hee-Mock Oh ◽  
Yong-Ha Park ◽  
Kang Hyun Lee ◽  
Haryoung Poo ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Phylogenetic Analyses ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
Wild Rabbit ◽  
Faecal Pellets ◽  
Phenotypic Differences ◽  
Taxonomic Methods ◽  
26S Rrna

Two previously undescribed anamorphic yeasts, strains T-11T and T-26T, recovered from wild rabbit faecal pellets collected in Muju, Korea, were identified using phenotypic and molecular taxonomic methods. The isolates were characterized by the proliferation of budding cells, positive diazonium blue B and urease reactions, the presence of Q-10 as the major ubiquinone, the presence of xylose in whole-cell hydrolysates and the inability to ferment sugars. Phylogenetic analyses based on 26S rRNA gene partial sequences revealed that strain T-11T was located in the Bulleromyces clade and was related to Sirobasidium intermedium, Tremella exigua, Cryptococcus cellulolyticus and Bullera pseudoalba. Strain T-26T was located in the Mesenterica clade and was closely related to Cryptococcus sp. F6 and Cryptococcus heveanensis CBS 8976. Sequence divergence values of more than 4 % from other described Cryptococcus species, together with the phenotypic differences, showed that the isolated yeasts represent previously unrecognized members of this genus. Therefore, two novel yeast species are proposed: Cryptococcus mujuensis sp. nov., with strain T-11T (=KCTC 17231T=CBS 10308T) as the type strain, and Cryptococcus cuniculi sp. nov., with strain T-26T (=KCTC 17232T=CBS 10309T) as the type strain.

scholarly journals Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil

2010 ◽  
Vol 60 (1) ◽  
pp. 244-248 ◽  
Author(s):  
Melissa Fontes Landell ◽  
Raisa Billodre ◽  
Jesus P. Ramos ◽  
Orílio Leoncini ◽  
Marilene H. Vainstein ◽  
...  
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
The Novel ◽  
D2 Domain ◽  
Its Gene ◽  
Ascomycetous Yeasts ◽  
Positive Results

Two novel yeast species, Candida aechmeae sp. nov. and Candida vrieseae sp. nov., were isolated from bromeliads in Itapuã Park, Rio Grande do Sul, Brazil. These species are genetically isolated from all other currently recognized ascomycetous yeasts based on their sequence divergence in the D1/D2 domain of the LSU rRNA gene. C. aechmeae sp. nov. is phylogenetically close to Candida ubatubensis, a species also isolated from bromeliads in Brazil, but the novel species can be differentiated on the basis of differences in the D1/D2 domain and positive results for the assimilation of l-arabinose, raffinose, inulin and citrate. Candida vrieseae sp. nov. is phylogenetically placed in a clade near Candida membranifaciens that is composed of several species associated with insects, but the novel species can be differentiated from them by the D1/D2 and ITS gene sequences, positive results for the assimilation of nitrite and a negative result for the assimilation of ethylamine. The type strain for Candida aechmeae sp. nov. is BI153T (=CBS 10831T=NRRL Y-48456T) and the type strain for C. vrieseae sp. nov. is BI146T (=CBS 10829T=NRRL Y-48461T).

The reassignment of three ‘lost’ Taphrina species (Taphrina bullata, Taphrina insititiae and Taphrina rhizophora) supported by the divergence of nuclear and mitochondrial DNA

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 3091-3098 ◽  
Author(s):  
Jana Petrýdesová ◽  
Kamila Bacigálová ◽  
Pavol Sulo
Keyword(s):  
Vascular Plants ◽  
Type Strain ◽  
Gene Sequence ◽  
Ribosomal Subunit ◽  
Sequence Divergence ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
As Species ◽  
Morphological And Physiological Traits ◽  
26S Rrna

The ascomycetous genus Taphrina Fries originally contained more than 90 phytopathogenic microscopic dimorphic ascomycetes causing specific infections in different vascular plants. Although species have mainly been identified historically according to their host and morphological and physiological traits, they can be studied and preserved in the form of yeasts arising from germinating ascospores. Due to low DNA sequence divergence and the lack of available strains, the number of accepted Taphrina species has currently been reduced to 28. The aim of this study is the description of three previously accepted species. Taphrina bullata (type strain CCY 58-4-1 = CBS 12783), Taphrina insititiae (type strain CCY 58-5-1 = CBS 12782) and Taphrina rhizophora (type strain CCY 58-6-1 = CBS 12781), which have been omitted from a recent key. The host range, the divergence of the 26S rRNA gene sequence, internal transcribed spacers (ITS) and the mitochondrial small ribosomal subunit (rns) sequence strongly support their reassignment as species.

Candida ficus sp. nov., a novel yeast species from the gut of Apriona germari larvae

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2805-2809 ◽  
Author(s):  
Feng-Li Hui ◽  
Qiu-Hong Niu ◽  
Tao Ke ◽  
Zheng Liu
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Central China ◽  
Rrna Gene ◽  
The Novel ◽  
Ascomycetous Yeasts ◽  
Large Subunit Rrna ◽  
Wood Boring

A novel yeast species is described based on three strains from the gut of wood-boring larvae collected in a tree trunk of Ficus carica cultivated in parks near Nanyang, central China. Phylogenetic analysis based on sequences of the D1/D2 domains of the large subunit rRNA gene showed that these strains occurred in a separate clade that was genetically distinct from all known ascomycetous yeasts. In terms of pairwise sequence divergence, the novel strains differed by 15.3 % divergence from the type strain of Pichia terricola, and by 15.8 % divergence from the type strains of Pichia exigua and Candida rugopelliculosa in the D1/D2 domains. All three are ascomycetous yeasts in the Pichia clade. Unlike P. terricola, P. exigua and C. rugopelliculosa, the novel isolates did not ferment glucose. The name Candida ficus sp. nov. is proposed to accommodate these highly divergent organisms, with STN-8T ( = CICC 1980T = CBS 12638T) as the type strain.

scholarly journals Candida asparagi sp. nov., Candida diospyri sp. nov. and Candida qinlingensis sp. nov., novel anamorphic, ascomycetous yeast species

2004 ◽  
Vol 54 (4) ◽  
pp. 1409-1414 ◽  
Author(s):  
Hui-Zhong Lu ◽  
Jian-Hua Jia ◽  
Qi-Ming Wang ◽  
Feng-Yan Bai
Keyword(s):  
Type Strain ◽  
Nature Reserve ◽  
Yeast Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Molecular Phylogenetic ◽  
Ascomycetous Yeasts ◽  
Close Relatives ◽  
26S Rrna

Among ascomycetous yeasts that were isolated from several nature reserve areas in China, three anamorphic strains isolated from soil (QL 5-5T) and fruit (QL 21-2T and SN 15-1T) were revealed, by conventional characterization and molecular phylogenetic analysis based on internal transcribed spacer and large subunit (26S) rRNA gene D1/D2 region sequencing, to represent three novel species in the genus Candida. Candida qinlingensis sp. nov. (type strain, QL 5-5T=AS 2.2524T=CBS 9768T) was related closely to a teleomorphic species, Williopsis pratensis. The close relatives of Candida diospyri sp. nov. (type strain, QL 21-2T=AS 2.2525T=CBS 9769T) are Candida friedrichii and Candida membranifaciens. Candida asparagi sp. nov. (type strain, SN 15-1T=AS 2.2526T=CBS 9770T) forms a clade with Candida fructus.

scholarly journals Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica)

2004 ◽  
Vol 54 (3) ◽  
pp. 925-928 ◽  
Author(s):  
Matthew D. Collins ◽  
Lesley Hoyles ◽  
Geoffrey Foster ◽  
Enevold Falsen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Facultative Anaerobe ◽  
Phenotypic Differences ◽  
Rrna Gene Sequencing ◽  
Caspian Seal

A previously unknown Gram-positive, non-spore-forming, non-lipophilic, catalase-positive, irregular rod-shaped bacterium (M/106/00/5T) was isolated, in mixed culture, from the penis of a Caspian seal (Phoca caspica). The strain was a facultative anaerobe that was able to grow at 22 and 42 °C. Comparative 16S rRNA gene sequencing showed that the organism formed a hitherto unknown subline within the genus Corynebacterium. Sequence divergence values of more than 5 % from other described Corynebacterium species, together with phenotypic differences, showed that the unidentified bacterium represents a previously unrecognized member of this genus. On the basis of phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium isolated from a Caspian seal (strain M/106/00/5T=CCUG 44566T=CIP 107965T) be classified as the type strain of a novel species of the genus Corynebacterium, Corynebacterium caspium sp. nov.

scholarly journals Leucosporidium drummii sp. nov., a member of the Microbotryomycetes isolated from soil

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 728-734 ◽  
Author(s):  
A. M. Yurkov ◽  
A. M. Schäfer ◽  
D. Begerow
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Rrna Gene ◽  
Grassland Soil ◽  
Core Group ◽  
The Core ◽  
26S Rrna ◽  
Phenotypic Tests

Two strains of a novel teleomorphic basidiomycete were isolated from grassland soil. Standard phenotypic tests and phylogenetic analyses of 26S rRNA gene (D1/D2 domains) and ITS region sequences showed that the species belongs to the core group of the genus Leucosporidium. A novel species, Leucosporidium drummii sp. nov., is proposed to accommodate the two strains, with SEG-3-2-AY220T ( = CBS 11562T = MUCL 52878T) as the type strain. In addition, phylogenetic analysis revealed great genetic variability in the Leucosporidium scottii complex.

Corynebacterium ureicelerivorans sp. nov., a lipophilic bacterium isolated from blood culture

2007 ◽  
Vol 57 (6) ◽  
pp. 1200-1203 ◽  
Author(s):  
A. F. Yassin
Keyword(s):  
Blood Culture ◽  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Phenotypic Differences ◽  
Mycolic Acids ◽  
Urease Test ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

A lipophilic coryneform bacterium isolated from a blood culture from a patient with signs of septicaemia was characterized by means of phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids, which are consistent with the genus Corynebacterium. The isolate was characterized biochemically by the very rapid (approx. 60 s) positive result that was obtained in a urease test in the API Coryne system. Comparative 16S rRNA gene sequencing demonstrated that the isolate belonged phylogenetically to the genus Corynebacterium. The values for sequence divergence (⩾1.4 %) with respect to known Corynebacterium species, together with phenotypic differences, show that the unidentified bacterium represents a novel member of this genus. On the basis of both the phenotypic and phylogenetic data, this isolate should be classified within a novel species of the genus Corynebacterium, for which the name Corynebacterium ureicelerivorans sp. nov. is proposed. The type strain is IMMIB RIV-2301T (=DSM 45051T=CCUG 53377T).

scholarly journals Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’

2004 ◽  
Vol 54 (4) ◽  
pp. 1129-1136 ◽  
Author(s):  
Jang-Cheon Cho ◽  
Stephen J. Giovannoni
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Phenotypic Differences ◽  
Monophyletic Lineage ◽  
Wide Range ◽  
Utilization Patterns ◽  
Dominant Fatty Acid

Three Gram-negative, chemoheterotrophic, non-motile, rod-shaped bacterial strains that accumulate poly-β-hydroxybutyrate granules were isolated from the Bermuda Atlantic Time-series Study site by high-throughput culturing methods and characterized by polyphasic approaches. DNA–DNA hybridization, DNA G+C content and phylogenetic analyses based on 16S rRNA gene sequences divided the three isolates into two distinct genospecies that were clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic susceptibility and biochemical characteristics. The strains utilized a wide range of substrates, including pentoses, hexoses, oligosaccharides, sugar alcohols, organic acids and amino acids. DNA G+C contents were 71·5, 70·9 and 67·3 mol% for strains HTCC2516T, HTCC2523 and HTCC2597T, respectively. The most dominant fatty acid was 18 : 1ω7c in strains HTCC2516T and HTCC2523, and cyclo 19 : 0 in strain HTCC2597T. The type strains HTCC2516T and HTCC2597T were clearly differentiated by the presence or absence of 12 : 0, 12 : 1ω11c, 14 : 0, 15 : 0 and methyl 18 : 1. Phylogenetic analyses indicated that the strains formed a distinct monophyletic lineage within the Roseobacter clade in the order ‘Rhodobacterales’ of the Alphaproteobacteria, and which did not associate with any of the described genera. Genotypic and phenotypic differences of the isolates from the previously described genera support the description of Oceanicola granulosus gen. nov., sp. nov. with the type strain HTCC2516T (=ATCC BAA-861T=DSM 15982T=KCTC 12143T) and of Oceanicola batsensis sp. nov. with the type strain HTCC2597T (=ATCC BAA-863T=DSM 15984T=KCTC 12145T).

scholarly journals Wickerhamiella kiyanii f.a., sp. nov. and Wickerhamiella fructicola f.a., sp. nov., two yeasts isolated from native plants of Atlantic rainforest in Brazil

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2152-2158 ◽  
Author(s):  
Ifeloju Dayo-Owoyemi ◽  
Carlos Augusto Rosa ◽  
André Rodrigues ◽  
Fernando Carlos Pagnocca
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Sequence Divergence ◽  
Atlantic Rainforest ◽  
Rrna Gene ◽  
The Novel ◽  
Taxonomic Descriptions ◽  
Lsu Rrna Gene ◽  
Brazilian Atlantic Rainforest

Two novel species, Wickerhamiella kiyanii f.a., sp. nov. (type strain FB1-1DASPT = CBS 12905T = CBMAI 1613T) and Wickerhamiella fructicola f.a., sp. nov. (type strain H10YT = CBS 12902T = CBMAI 1614T) are proposed in the Wickerhamiella clade (Saccharomycetes, Saccharomycetales) to accommodate three strains isolated from flowers and fruits typical of the Brazilian Atlantic rainforest. The novel status of these yeast species was established by sequence divergence observed in the D1/D2 domains of the LSU rRNA gene from the most closely related, described species as well as by phylogenetic analysis. Wickerhamiella kiyanii sp. nov. differs from its nearest phylogenetic neighbours W. pagnoccae CBS 12178T, Candida jalapaonensis CBS 10935T and Candida drosophilae CBS 8459T by 2.2–4.2 % in the D1/D2 sequences. By contrast, a sequence divergence of 13.2–13.8 % was observed between W. fructicola sp. nov. and its closest, described phylogenetic relative Candida kazoui JCM 12558T and Candida hasegawae JCM 12559T. Taxonomic descriptions of the two novel species are given.

scholarly journals Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2875-2881 ◽  
Author(s):  
Yong-Cheng Ren ◽  
Si-Tong Liu ◽  
Ying Li ◽  
Feng-Li Hui
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
Insect Larvae ◽  
Rotten Wood ◽  
Yeast Strains ◽  
Lsu Rrna Gene ◽  
Established Species

Seven yeast strains were isolated from the gut of insect larvae and decayed wood, which were collected from three localities near Nanyang, Henan Province, China. These strains were identified as two novel species through comparison of sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and other taxonomic characteristics. Pichia dushanensis sp. nov. was closely related to species in the Pichia clade and produced one to four spheroid ascospores in a deliquescent ascus. The D1/D2 sequence of P. dushanensis sp. nov. differed from its closest relative, Issatchenkia (Pichia) sp. NRRL Y-12824, by 3.6  % sequence divergence (16 substitutions and 4 gaps). The species also differed from its four closest known species, Candida rugopelliculosa, Pichia occidentalis, Pichia exigua and Candida phayaonensis, by 4.1–4.4  % sequence divergence (22–24 substitutions and 0–2 gaps) in the D1/D2 sequences. Hyphopichia paragotoi sp. nov. belonged to the Hyphopichia clade, and its nearest phylogenetic neighbours were Candida gotoi, Candida pseudorhagii, Candida rhagii and Hyphopichia heimii with 3.2–4.2  % sequence divergence (16–21substitutions and 1 gap) in the D1/D2 sequences. In comparison with previously established species, H. paragotoi sp. nov. formed one hat-shaped ascospore in a persistent ascus. The type strain of P. dushanensis sp. nov. is NYNU 14658T ( = CICC 33049T = CBS 13912T), and the type strain of H. paragotoi sp. nov. is NYNU 14666T ( = CICC 33048T = CBS 13913T).

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