Corynebacterium ureicelerivorans sp. nov., a lipophilic bacterium isolated from blood culture

2007 ◽  
Vol 57 (6) ◽  
pp. 1200-1203 ◽  
Author(s):  
A. F. Yassin
Keyword(s):  
Blood Culture ◽  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Phenotypic Differences ◽  
Mycolic Acids ◽  
Urease Test ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

A lipophilic coryneform bacterium isolated from a blood culture from a patient with signs of septicaemia was characterized by means of phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids, which are consistent with the genus Corynebacterium. The isolate was characterized biochemically by the very rapid (approx. 60 s) positive result that was obtained in a urease test in the API Coryne system. Comparative 16S rRNA gene sequencing demonstrated that the isolate belonged phylogenetically to the genus Corynebacterium. The values for sequence divergence (⩾1.4 %) with respect to known Corynebacterium species, together with phenotypic differences, show that the unidentified bacterium represents a novel member of this genus. On the basis of both the phenotypic and phylogenetic data, this isolate should be classified within a novel species of the genus Corynebacterium, for which the name Corynebacterium ureicelerivorans sp. nov. is proposed. The type strain is IMMIB RIV-2301T (=DSM 45051T=CCUG 53377T).

scholarly journals Corynebacterium caspium sp. nov., from a Caspian seal (Phoca caspica)

2004 ◽  
Vol 54 (3) ◽  
pp. 925-928 ◽  
Author(s):  
Matthew D. Collins ◽  
Lesley Hoyles ◽  
Geoffrey Foster ◽  
Enevold Falsen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Facultative Anaerobe ◽  
Phenotypic Differences ◽  
Rrna Gene Sequencing ◽  
Caspian Seal

A previously unknown Gram-positive, non-spore-forming, non-lipophilic, catalase-positive, irregular rod-shaped bacterium (M/106/00/5T) was isolated, in mixed culture, from the penis of a Caspian seal (Phoca caspica). The strain was a facultative anaerobe that was able to grow at 22 and 42 °C. Comparative 16S rRNA gene sequencing showed that the organism formed a hitherto unknown subline within the genus Corynebacterium. Sequence divergence values of more than 5 % from other described Corynebacterium species, together with phenotypic differences, showed that the unidentified bacterium represents a previously unrecognized member of this genus. On the basis of phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium isolated from a Caspian seal (strain M/106/00/5T=CCUG 44566T=CIP 107965T) be classified as the type strain of a novel species of the genus Corynebacterium, Corynebacterium caspium sp. nov.

scholarly journals Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug

2005 ◽  
Vol 55 (4) ◽  
pp. 1575-1579 ◽  
Author(s):  
A. F. Yassin
Keyword(s):  
Dna Hybridization ◽  
Novel Species ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Mycolic Acids ◽  
Blood Sucking ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

Two bacterial isolates, strains IMMIB RIV-085T and IMMIB RIV-095, isolated from a blood-sucking bug of the genus Triatoma, were characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and mycolic acids consistent with the genus Rhodococcus. Comparative 16S rRNA gene sequencing showed that the two isolates are genealogically highly related (100 % sequence similarity) and constitute a new subline within the genus Rhodococcus, with Rhodococcus corynebacteroides and Rhodococcus rhodnii as their nearest phylogenetic neighbours (98·4 and 98·3 % sequence similarity, respectively). However, DNA–DNA hybridization experiments demonstrated unambiguously that the isolates are genealogically distinct from R. corynebacteroides and R. rhodnii (32 and 43 % relatedness, respectively). The isolates could be distinguished from their phylogenetic relatives and other members of the genus Rhodococcus by means of biochemical tests. On the basis of both phenotypic and phylogenetic evidence, it is proposed that these isolates be classified as a novel species, Rhodococcus triatomae sp. nov. The type strain is strain IMMIB RIV-085T (=CCUG 50202T=DSM 44892T).

scholarly journals Streptococcus marimammalium sp. nov., isolated from seals

2005 ◽  
Vol 55 (1) ◽  
pp. 271-274 ◽  
Author(s):  
Paul A. Lawson ◽  
Geoffrey Foster ◽  
Enevold Falsen ◽  
Matthew D. Collins
Keyword(s):  
Novel Species ◽  
Molecular Genetic ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sequencing Studies ◽  
Streptococcal Species ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing ◽  
Molecular Genetic Evidence

Two strains of an unidentified, Gram-positive, catalase-negative, chain-forming, coccus-shaped organism recovered from seals were characterized using phenotypic and molecular taxonomic methods. Based on morphological and biochemical criteria the strains were tentatively identified as streptococci but they did not appear to correspond to any recognized species of the genus Streptococcus. Comparative 16S rRNA gene sequencing studies showed that the strains were closely related to each other and confirmed their placement in the genus Streptococcus. Sequence divergence values of >5 % with reference streptococcal species demonstrated the organisms from seals represent a novel species. SDS-PAGE analysis of whole-cell proteins confirmed that the two organisms were closely related to each other but were different from all currently defined streptococcal species. Based on biochemical criteria, molecular chemical and molecular genetic evidence, it is proposed that the unknown isolates from seals be assigned to a novel species of the genus Streptococcus, Streptococcus marimammalium sp. nov. The type strain is M54/01/1T (=CCUG 48494T=CIP 108309T).

scholarly journals Sutterella stercoricanis sp. nov., isolated from canine faeces

2004 ◽  
Vol 54 (5) ◽  
pp. 1581-1584 ◽  
Author(s):  
Hazel L. Greetham ◽  
Matthew D. Collins ◽  
Glenn R. Gibson ◽  
Catriona Giffard ◽  
Enevold Falsen ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Molecular Genetic ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Anaerobic Conditions ◽  
Genetic Studies ◽  
Rrna Gene Sequencing

Morphological, biochemical and molecular genetic studies were carried out on an unknown non-spore-forming, Gram-negative, rod-shaped bacterium which was isolated from dog faeces. The bacterium grew under anaerobic conditions, was asaccharolytic, resistant to 20 % (v/v) bile and was oxidase- and urease-negative. Phylogenetic analysis based on comparative 16S rRNA gene sequencing showed that the unidentified bacterium clustered with Sutterella wadsworthensis, although a sequence divergence of >5 % indicated that the bacterium from dog faeces represented a previously unrecognized subline within the genus. On the basis of the presented findings, a novel species, Sutterella stercoricanis sp. nov., is described. The type strain of Sutterella stercoricanis is 5BAC4T (=CCUG 47620T=CIP 108024T).

scholarly journals Actinomyces dentalis sp. nov., from a human dental abscess

2005 ◽  
Vol 55 (1) ◽  
pp. 427-431 ◽  
Author(s):  
Val Hall ◽  
Matthew D. Collins ◽  
Paul A. Lawson ◽  
Enevold Falsen ◽  
Brian I. Duerden
Keyword(s):  
Novel Species ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Dental Abscess ◽  
Unknown Species ◽  
Sequencing Studies ◽  
Molecular Phylogenetic ◽  
Rrna Gene Sequencing ◽  
Biochemical Testing

A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus Actinomyces, but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus Actinomyces, clustering within a group of species that includes Actinomyces bovis, the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, Actinomyces dentalis sp. nov. The type strain is R18165T (=CCUG 48064T=CIP 108337T).

scholarly journals Murdochiella asaccharolytica gen. nov., sp. nov., a Gram-stain-positive, anaerobic coccus isolated from human wound specimens

2010 ◽  
Vol 60 (5) ◽  
pp. 1013-1016 ◽  
Author(s):  
Nurver Ulger-Toprak ◽  
Chengxu Liu ◽  
Paula H. Summanen ◽  
Sydney M. Finegold
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Gram Stain ◽  
Sequencing Studies ◽  
Novel Genus And Species ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

Two strains of previously unknown Gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, WAL 1855CT and WAL 2038E, are genotypically homogeneous and constitute a novel lineage within Clostridium cluster XIII. There was 13–14 % 16S rRNA gene sequence divergence between the novel strains and the most closely related species, Parvimonas micra, Finegoldia magna and species of Helcococcus. Based on the phenotypic and phylogenetic findings, a novel genus and species, Murdochiella asaccharolytica gen. nov., sp. nov., are proposed. Strain WAL 1855CT (=ATCC BAA-1631T =CCUG 55976T) is the type strain of Murdochiella asaccharolytica.

scholarly journals Nocardia polyresistens sp. nov.

2005 ◽  
Vol 55 (4) ◽  
pp. 1465-1470 ◽  
Author(s):  
Ping Xu ◽  
Wen-Jun Li ◽  
Shu-Kun Tang ◽  
Yi Jiang ◽  
Hua-Hong Chen ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Actinomycete Strain ◽  
Rrna Gene Sequencing ◽  
Genotypic Characteristics ◽  
Established Species

A novel actinomycete strain YIM 33361T was isolated from a soil sample collected from Yunnan, China. Comparative 16S rRNA gene sequencing showed that the strain constituted a distinct subclade within the genus Nocardia, displaying more than 3 % sequence divergence from established species. Based on its morphological, chemotaxonomic, phenotypic and genotypic characteristics, strain YIM 33361T (=CCTCC AA 204004T=KCTC 19027T) is proposed as the type strain of a novel species, Nocardia polyresistens sp. nov.

scholarly journals Bacteroides coprosuis sp. nov., isolated from swine-manure storage pits

2005 ◽  
Vol 55 (6) ◽  
pp. 2515-2518 ◽  
Author(s):  
Terence R. Whitehead ◽  
Michael A. Cotta ◽  
Matthew D. Collins ◽  
Enevold Falsen ◽  
Paul A. Lawson
Keyword(s):  
Novel Species ◽  
Molecular Genetic ◽  
Swine Manure ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sequencing Studies ◽  
Manure Storage ◽  
Rrna Gene Sequencing ◽  
Molecular Genetic Evidence

Two Gram-negative, anaerobic, non-spore-forming, rod-shaped organisms were isolated from a swine-manure storage pit. Based on morphological and biochemical criteria, the strains were tentatively identified as belonging to the genus Bacteroides but they did not appear to correspond to any recognized species of the genus. Comparative 16S rRNA gene sequencing studies showed that the strains were related closely to each other and confirmed their placement in the genus Bacteroides, but sequence divergence values of >10 % from reference Bacteroides species demonstrated that the organisms from manure represent a novel species. Based on biochemical criteria and molecular genetic evidence, it is proposed that the unknown isolates from manure be assigned to a novel species of the genus Bacteroides, as Bacteroides coprosuis sp. nov. The type strain is PC139T (=CCUG 50528T=NRRL B-41113T).

scholarly journals Atopococcus tabaci gen. nov., sp. nov., a novel Gram-positive, catalase-negative, coccus-shaped bacterium isolated from tobacco

2005 ◽  
Vol 55 (4) ◽  
pp. 1693-1696 ◽  
Author(s):  
Matthew D. Collins ◽  
Anna Wiernik ◽  
Enevold Falsen ◽  
Paul A. Lawson
Keyword(s):  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
New Genus And Species ◽  
Gram Positive ◽  
Sequencing Studies ◽  
Molecular Phylogenetic ◽  
A Cell ◽  
Taxonomic Methods ◽  
Rrna Gene Sequencing

A novel Gram-positive, aerobic, catalase-negative, coccus-shaped organism originating from tobacco was characterized using phenotypic and molecular taxonomic methods. The organism contained a cell wall murein based on l-lysine (variation A4α, type l-lysine–l-glutamic acid), synthesized long-chain cellular fatty acids of the straight-chain saturated and monounsaturated types (with C16 : 1 ω9, C16 : 0 and C18 : 1 ω9 predominating) and possessed a DNA G+C content of 46 mol%. Based on morphological, biochemical and chemical characteristics, the coccus-shaped organism did not conform to any presently recognized taxon. Comparative 16S rRNA gene sequencing studies confirmed the distinctiveness of the unknown coccus, with the bacterium displaying sequence divergence values of greater than 7 % with other recognized Gram-positive taxa. Treeing analysis reinforced its distinctiveness, with the unidentified organism forming a relatively long subline branching at the periphery of an rRNA gene sequence cluster which encompasses the genera Alloiococcus, Allofustis, Alkalibacterium, Atopostipes, Dolosigranulum and Marinilactibacillus. Based on phenotypic and molecular phylogenetic evidence, it is proposed that the unknown organism from tobacco be classified as a new genus and species, Atopococcus tabaci gen. nov., sp. nov. The type strain of Atopococcus tabaci is CCUG 48253T (=CIP 108502T).

scholarly journals Jeotgalicoccus pinnipedialis sp. nov., from a southern elephant seal (Mirounga leonina)

2004 ◽  
Vol 54 (3) ◽  
pp. 745-748 ◽  
Author(s):  
Lesley Hoyles ◽  
Matthew D. Collins ◽  
Geoffrey Foster ◽  
Enevold Falsen ◽  
Peter Schumann
Keyword(s):  
Sequence Divergence ◽  
16S Rrna Gene Sequencing ◽  
Elephant Seal ◽  
Southern Elephant Seal ◽  
Rrna Gene ◽  
Mirounga Leonina ◽  
Gram Positive ◽  
Phenotypic Differences ◽  
Rrna Gene Sequencing ◽  
Specific Association

A previously unknown Gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium (A/G14/99/10T), originating from the mouth of a female southern elephant seal, was subjected to a taxonomic analysis. Comparative 16S rRNA gene-sequencing showed that the organism formed a hitherto unknown subline within the catalase-positive, low-G+C, Gram-positive cocci, exhibiting a specific association with species of the genus Jeotgalicoccus. Sequence divergence values of approximately 7 %, together with phenotypic differences, showed the unknown bacterium to be distinct from the two described species of this genus, Jeotgalicoccus halotolerans and Jeotgalicoccus psychrophilus. Based on phenotypic and phylogenetic considerations, it is proposed that strain A/G14/99/10T=CCUG 42722T=CIP 107946T from the mouth of a seal be classified as the type strain of a novel species of the genus Jeotgalicoccus, Jeotgalicoccus pinnipedialis sp. nov.

Sign in / Sign up

Export Citation Format

Share Document