One Bat’s Waste is Another Man’s Treasure: A DNA Metabarcoding Approach for the Assessment of Biodiversity and Ecosystem Services Using Bat Faeces

Arthropod populations are constantly changing due to changes in climate and the globalisation of trade and travel. Effective and diverse monitoring techniques are required to understand these changes. DNA metabarcoding has facilitated the development of a broad monitoring method to sample arthropod diversity from environmental and faecal samples. In this study, we applied DNA metabarcoding to DNA extracted from bat faecal pellets of Rhinolophus hipposideros, the lesser horseshoe bat in Ireland, a highly protected bat species of conservation concern in Europe. From as few as 24 bat faecal pellets, we detected 161 arthropod species, spanning 11 orders, including 38 pest species of which five were determined to be priority pests, highlighting important ecosystem services. We also report the identification 14 species not previously reported in Ireland, but upon further investigation found that many of these are likely misidentified due to inadequacies in the genetic reference database. For the first time, we were able to use non-invasively collected bat samples to examine the role of sex in the diet of bats and found that the male and female diets did not differ significantly. However, sampling location did explain variation within the diet, highlighting how landscape features influence arthropod composition and diversity. We discuss the current limitations of the methodology in Ireland, how these can be overcome in future studies, and how this data can be used for biodiversity monitoring and informing conservation management of protected bat species.

Termite coprolites (Blattodea: Isoptera) from the Early Cretaceous of eastern Inner Mongolia, Northeast China

Well-preserved coprolites (fossil faecal pellets) were found from lignite seams of the Lower Cretaceous Huolinhe Formation at the Huolinhe Basin in eastern Inner Mongolia, Northeast China. These coprolites provide a combination of following features: oval to cylindrical shaped with six longitudinal ridges, hexagonal to elliptical cross-sections, and one blunt end and the other pointed end. According to these distinct features and their size range, the producers of these coprolites are attributed to termites. Termites were estimated to have originated in the earliest Cretaceous with an evolutionary radiation in the Early Cretaceous. The presence of wood debris in the coprolites indicate that the Early Cretaceous termites from the Huolinhe Basin had wood-feeding habits; and anatomical features displaying on the wood debris further suggest their feeding preference was coniferous wood. Besides, the results of a k-means clustering analysis performed for these coprolites indicate that three clusters with different proportion were present, suggesting the division of labor in termites’ sociality existed as early as the Early Cretaceous.

Genetic identification of bat species for pathogen surveillance across France

With more than 1400 chiropteran species identified to date, bats comprise one-fifth of all mammalian species worldwide. Many studies have associated viral zoonoses with 45 different species of bats in the EU, which cluster within 5 families of bats. For example, the Serotine bats are infected by European Bat 1 Lyssavirus throughout Europe while Myotis bats are shown infected by coronavirus, herpesvirus and paramyxovirus. Correct host species identification is important to increase our knowledge of the ecology and evolutionary pattern of bat viruses in the EU. Bat species identification is commonly determined using morphological keys. Morphological determination of bat species from bat carcasses can be limited in some cases, due to the state of decomposition or nearly indistinguishable morphological features in juvenile bats and can lead to misidentifications. The overall objective of our study was to identify insectivorous bat species using molecular biology tools with the amplification of the partial cytochrome b gene of mitochondrial DNA. Two types of samples were tested in this study, bat wing punches and bat faeces. A total of 163 bat wing punches representing 22 species, and 31 faecal pellets representing 7 species were included in the study. From the 163 bat wing punches tested, a total of 159 were genetically identified from amplification of the partial cyt b gene. All 31 faecal pellets were genetically identified based on the cyt b gene. A comparison between morphological and genetic determination showed 21 misidentifications from the 163 wing punches, representing ~12.5% of misidentifications of morphological determination compared with the genetic method, across 11 species. In addition, genetic determination allowed the identification of 24 out of 25 morphologically non-determined bat samples. Our findings demonstrate the importance of a genetic approach as an efficient and reliable method to identify bat species precisely.

Gut Microbiota-Derived PGF2α Fights against Radiation-Induced Lung Toxicity through the MAPK/NF-κB Pathway

Radiation pneumonia is a common and intractable side effect associated with radiotherapy for chest cancer and involves oxidative stress damage and inflammation, prematurely halting the remedy and reducing the life quality of patients. However, the therapeutic options for the complication have yielded disappointing results in clinical application. Here, we report an effective avenue for fighting against radiation pneumonia. Faecal microbiota transplantation (FMT) reduced radiation pneumonia, scavenged oxidative stress and improved lung function in mouse models. Local chest irradiation shifted the gut bacterial taxonomic proportions, which were preserved by FMT. The level of gut microbiota-derived PGF2α decreased following irradiation but increased after FMT. Experimental mice with PGF2α replenishment, via an oral route, exhibited accumulated PGF2α in faecal pellets, peripheral blood and lung tissues, resulting in the attenuation of inflammatory status of the lung and amelioration of lung respiratory function following local chest irradiation. PGF2α activated the FP/MAPK/NF-κB axis to promote cell proliferation and inhibit apoptosis with radiation challenge; silencing MAPK attenuated the protective effect of PGF2α on radiation-challenged lung cells. Together, our findings pave the way for the clinical treatment of radiotherapy-associated complications and underpin PGF2α as a gut microbiota-produced metabolite.

Krill and salp faecal pellets contribute equally to the carbon flux at the Antarctic Peninsula

Krill and salps are important for carbon flux in the Southern Ocean, but the extent of their contribution and the consequences of shifts in dominance from krill to salps remain unclear. We present a direct comparison of the contribution of krill and salp faecal pellets (FP) to vertical carbon flux at the Antarctic Peninsula using a combination of sediment traps, FP production, carbon content, microbial degradation, and krill and salp abundances. Salps produce 4-fold more FP carbon than krill, but the FP from both species contribute equally to the carbon flux at 300 m, accounting for 75% of total carbon. Krill FP are exported to 72% to 300 m, while 80% of salp FP are retained in the mixed layer due to fragmentation. Thus, declining krill abundances could lead to decreased carbon flux, indicating that the Antarctic Peninsula could become a less efficient carbon sink for anthropogenic CO2 in future.

Over time Decay of Cortisol Metabolites in Faecal Pellets of Koalas in Central Queensland

Faecal material can be a valuable source of information for a range of animal health aspects and can be used to measure faecal cortisol metabolites (FCMs). FCM values can relate to physiological stress responses. However, freshly defecated pellets are not always available and environmental conditions, such as temperature and humidity, might affect faecal pellet consistency and FCM levels. Therefore, the impact of environmental conditions on FCMs needs to be evaluated. We collected 107 pellets from two female and two male koalas, exposed them to three types of treatments, and analysed FCMs in these samples with three enzyme immunoassays (EIAs). After analysis, the original FCM values were mathematically corrected for water loss. Results show that the FCMs were more stable when measured using tetrahydrocorticosterone (50c) and 5α-pregnane-3β,11β,21-triol-20-one (37e) EIAs, and were less stable when measured with the cortisol EIA. With 50c, the FCM values did not vary significantly over time either before or after the adjustment with water in the environment treatment group. For samples kept under constant low (25 °C) and high (35 °C) temperatures, the 50c FCM values did not vary significantly over time, after adjustments were made for water loss. Thus, this study highlights the importance of considering the suitability of faecal field samples for FCM analysis. Because water loss was the main driver of FCM changes, we strongly recommend collecting koala pellets that are freshly defecated, despite the effort and time it might take to collect such pellets.

Highly Active Antiretroviral Therapy-silver Nanoparticle Conjugate Alleviates Anxiety-like Behaviour in Streptozotocin-induced Diabetic Rats

Background: The inception of highly active antiretroviral therapy (HAART) has changed the management of human immunodeficiency virus (HIV) positive patients, and their life expectancy has improved. However, neurological complications associated with chronic HAART administration has not been fully addressed. Therefore, this study aimed to evaluate the potential benefits of silver nanoparticles (AgNPs) conjugates on the anxiogenic effects of HAART in type-2 diabetic rats. Methods: Forty-two (42) adult male Sprague-Dawley rats (250 ± 13 g) were divided into non-diabetic and diabetic groups. After induction of diabetes, non-diabetic and diabetic animals were administered either with de-ionized water (DW), HAART (98.2 mg/kg, p.o) or AgNPs +HAART (24.5 mg/kg, i. p) for 8 weeks. Thereafter, metabolic biomarkers, oxidative injury, tissue inflammation, and behavioural changes were evaluated, while the prefrontal cortex was excised for neurochemical analysis. Results: The HAART-treated diabetic rats showed a significant increase in blood glucose level, number of faecal pellets, malondialdehyde (MDA), and pro-inflammatory cytokines (TNF-α, IL-1β) while locomotion, reduced glutathione (GSH), superoxide dismutase (SOD) activity, and PFC-GFAP positive cells were significantly reduced compared with diabetic control. However, administration of AgNPs +HAART to diabetic rats significantly improved the blood glucose level, metabolic activities, SOD, GSH, PFC-GFAP positive cells while reducing MDA and anxiety-like behaviour in the open field test. Conclusion: Administration of HAART aggravates anxiety-like behaviours and promotes neurotoxic effects in the PFC of diabetic rats. However, AgNPs +HAART alleviates the 3 anxiogenic effects of HAART and preserves PFC-GFAP positive cells by reducing oxidative and neuroinflammatory injury.

Manure Pulverizers and Applicators: A Review

Background: Manures (FYM, vermicompost, edible oil cakes etc.) are important resources which provide nutrients that could reduce bagged fertilizer costs and improves the crop growth and performance. A well-managed manure is a valuable resource in providing nutrients for crop production. As manure dries, nutrients not only get concentrated on a weight basis, but also on a volume basis due to structural changes. Compared to fresh manure, it is easier to handle and transport because of decreased volume and weight. Hence, it is necessary to pulverize the manure when it is applied to the land. Methods: Various research works previously carried out on manure pulverizers and applicators have been considered in the study to come up with an idea of developing a tractor powered manure pulverizer cum applicator. Studies concluded the importance of manure pulverization, effect of wind, angle of repose and coefficient of friction in the designing and development of manure dispensing equipment. Result: Manure pulverizer integrated with tractor PTO along with an applicator was designed and developed. The machine consists of KAU manure pulverizer, feed chute, blower, frame and hitch, gearbox and extension shaft. Power to the gearbox was drawn from the tractor PTO. Dried manure was continuously fed through the feeding chute and rotating blade helps in pulverizing the manure until it achieves a size smaller than the sieve. The pulverized manure reaches the blower unit by means of a chute and gets discharged through the flexible pipes. Maximum field capacity was noted at a traveling speed of 3.0 km h-1. A larger application rate of 1387.1 kg ha-1 for cow dung, 1624.4 kg ha-1 for goat faecal pellets and 1618.6 kg ha-1 for neem cake was noted at an engine rpm of 2500, forward speed of 2 km h-1 with a field capacity of 0.31 ha h-1.

Population Studies of Deer (Odocoileus virginianus, Mazama americana) in Southern Yucatán, Mexico

Aims: Estimate the population density of deer in the municipality of Tzucacab, Yucatán in the periods of 2003-2004, 2007-2008 and 2008-2009, determine the use of the habitat by these populations and the sustainability of the deer harvest from the estimated population densities. Study Design: A descriptive and vertical free-living deer population study was carried out in southern Yucatan, Mexico over a three-year period. Methodology: The map of the municipality of Tuzcacab was zoned in quadrants of 36 km2, completing a total of 36 quadrants; Unrestricted random sampling was applied to select seven quadrants in the period from 2003 to 2004 and 18 in each annual period between 2007 and 2009. Population samplings were carried out by applying three population estimation methods: direct sighting in a linear transect of 5 km in length, count of tracks in transect except period 2003-2004 and faecal pellets group count in plots. The evaluation of the use of habitat was carried out using the Bonferroni intervals, from the data of faecal pellets count. The evaluation of the deer harvest was carried out using the sustainable harvest model. Results: The population densities were different in each method, the density by the excreta count was 4.63 ± 2.49 deer / km2 in 2003-2004, 0.294 ± 0.198 deer / km2 in 2007-2008, and in the year 2008-2009 was 0.419 ± 0.0000085 deer / km2. Habitat use in 2007-08 and 2008-2009 was higher in the tropical forest, lower in agriculture and similar to that expected in secondary succession forest (acahual). The values of sustainable harvest, taking as a value the density per count of excreta in the plot because it showed the highest statistical precision, in the period 2003-04 it is sustainable, but in the period from 2007 to 2009 it is not sustainable. Conclusion: The population densities of deer (O. virginianus and M. americana) in Tuzcacab by means of the excreta count method, have decreased significantly. The habitat use preference is the tropical forest. The deer harvest in the period from 2007 to 2009 is not sustainable.