scholarly journals Runella limosa sp. nov., isolated from activated sludge

2006 ◽  
Vol 56 (12) ◽  
pp. 2757-2760 ◽  
Author(s):  
Seung Hyun Ryu ◽  
Thi Thu Hien Nguyen ◽  
Woojun Park ◽  
Chang-Jin Kim ◽  
Che Ok Jeon
Keyword(s):  
Activated Sludge ◽  
Sequencing Batch Reactor ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Batch Reactor ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Biological Phosphorus Removal ◽  
The Family ◽  
Biological Phosphorus

A Gram-negative bacterium, designated strain EMB111T, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Cells were long and rod-shaped. The isolate was strictly aerobic and non-motile. The strain grew optimally at 25–30 °C and pH 7.5–8.0. The predominant fatty acids of strain EMB111T were iso-C15 : 0, C16 : 1 ω5c, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH, C16 : 0 3-OH, C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The strain contained a large amount of phosphatidylglycerol and small amounts of two unknown phospholipids (PL1, PL2) as the polar lipids. The major isoprenoid quinone was menaquinone-7. The G+C content of the genomic DNA was 42.7 mol%. Phylogenetic analysis showed that strain EMB111T formed a phyletic cluster with members of the genus Runella within the family Flexibacteraceae and was most closely related to Runella slithyformis ATCC 29530T with a 16S rRNA gene sequence similarity of 94.8 %. On the basis of chemotaxonomic data and molecular properties, strain EMB111T represents a novel species within the genus Runella, for which the name Runella limosa sp. nov. is proposed. The type strain is EMB111T (=KCTC 12615T=DSM 17973T).

scholarly journals Flavobacterium croceum sp. nov., isolated from activated sludge

2006 ◽  
Vol 56 (10) ◽  
pp. 2443-2447 ◽  
Author(s):  
Minjeong Park ◽  
Shipeng Lu ◽  
Seung Hyun Ryu ◽  
Bok Sil Chung ◽  
Woojun Park ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Activated Sludge ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Batch Reactor ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Rrna Gene Sequence

A Gram-negative, non-motile, rod-shaped bacterium, designated strain EMB47T, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Growth was observed between 10 and 40 °C (optimum, 25–35 °C) and between pH 5.0 and 8.5 (optimum, pH 7.5–8.0). The predominant fatty acids of strain EMB47T were iso-C16 : 0 3-OH, iso-C15 : 1 G, C15 : 0, iso-C15 : 0, iso-C14 : 0 and iso-C16 : 0 and it contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine as polar lipids. The G+C content of the genomic DNA was 40.8 mol% and the major quinone was MK-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB47T formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with respect to Flavobacterium species were below 94.7 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB47T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium croceum sp. nov. is proposed. The type strain is EMB47T (=KCTC 12611T=DSM 17960T).

scholarly journals Hydrogenophaga caeni sp. nov., isolated from activated sludge

2007 ◽  
Vol 57 (5) ◽  
pp. 1126-1130 ◽  
Author(s):  
Bok Sil Chung ◽  
Seung Hyun Ryu ◽  
Minjeong Park ◽  
Yeji Jeon ◽  
Young Ryun Chung ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Activated Sludge ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Batch Reactor ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Rrna Gene Sequence

A Gram-negative bacterium, designated strain EMB71T, was isolated from activated sludge used for enhanced biological phosphorus removal in a sequencing batch reactor. The cells of the isolate were facultatively aerobic, motile rods with single polar flagella. Growth was observed to occur at 15–35 °C (optimally at 30 °C) and at pH 6.0–9.0 (optimally at pH 7.0–8.0). The predominant fatty acids of strain EMB71T were C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), and the polar lipids comprised a large amount of phosphatidylethanolamine and a small amount of diphosphatidylglycerol. The G+C content of the genomic DNA was 61.6 mol % and the major quinone was Q-8. Comparative 16S rRNA gene sequence analyses showed that strain EMB71T formed a phyletic lineage with the genus Hydrogenophaga within the family Comamonadaceae. The levels of 16S rRNA gene sequence similarity with respect to the type strains of Hydrogenophaga species ranged from 95.1 to 96.9 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB71T represents a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga caeni sp. nov. is proposed. The type strain is EMB71T (=KCTC 12613T=DSM 17962T).

scholarly journals Brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge

2007 ◽  
Vol 57 (7) ◽  
pp. 1561-1565 ◽  
Author(s):  
Seung Hyun Ryu ◽  
Minjeong Park ◽  
Jung Ro Lee ◽  
Pil-Yong Yun ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Activated Sludge ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Batch Reactor ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Rrna Gene Sequence

A Gram-negative, rod-like, stalk-producing bacterium, designated strain EMB102T, was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. Cells without stalks were motile with single polar flagella, but cells that did produce stalks were non-motile and lacked polar flagella. Growth of strain EMB102T was observed at temperatures between 15 and 35 °C (optimum, 30 °C) and between pH 6.0 and 9.0 (optimum, pH 7.5–8.5). The predominant fatty acids of strain EMB102T were C18 : 1 ω7c, C16 : 0 and C15 : 0. The predominant polar lipid was phosphatidylglycerol. The G+C content of the genomic DNA was 64.1 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analyses showed that strain EMB102T formed a distinct phyletic lineage within the genus Brevundimonas. The levels of 16S rRNA gene sequence similarity between the type strains of Brevundimonas species ranged from 95.8 to 97.5 %. DNA–DNA relatedness levels between the EMB102T and closely related Brevundimonas species were below 15.0 %. On the basis of chemotaxonomic data and molecular properties, strain EMB102T represents a novel species within the genus Brevundimonas, for which the name Brevundimonas aveniformis sp. nov. is proposed. The type strain is EMB102T (=KCTC 12609T=DSM 17977T).

scholarly journals Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent

2010 ◽  
Vol 60 (12) ◽  
pp. 2780-2785 ◽  
Author(s):  
Ying Liu ◽  
Jing-Hua Jin ◽  
Ying-Hao Liu ◽  
Yu-Guang Zhou ◽  
Zhi-Pei Liu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Malachite Green ◽  
Sequencing Batch Reactor ◽  
Sequence Similarity ◽  
Batch Reactor ◽  
Membrane System ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Family

A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22T, was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22T were slightly curved to straight rods (0.3–0.5×0.6–1.0 μm) and motile by a single polar flagellum. Strain LM22T was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll a were absent. Growth occurred at 20–40 °C (optimum 30–35 °C) and pH 6.0–10.0 (optimum pH 7.0–7.5). Strain LM22T did not require NaCl for growth and tolerated up to 2.0 % NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10 % of the total) were C18 : 1 ω7c (32.9 %), C19 : 0 cyclo ω8c (18.7 %), C16 : 0 (12.1 %) and C16 : 0 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that Inquilinus limosus AU0476T was the closest relative (90.4 % 16S rRNA gene sequence similarity). The DNA G+C content was 65.6 mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22T was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain of Dongia mobilis is LM22T (=CGMCC 1.7660T =JCM 15798T).

Simplicispira limi sp. nov., isolated from activated sludge

2007 ◽  
Vol 57 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Shipeng Lu ◽  
Seung Hyun Ryu ◽  
Bok Sil Chung ◽  
Young Ryun Chung ◽  
Woojun Park ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Novel Species ◽  
Batch Reactor ◽  
Molecular Characteristics ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
16S Rrna Gene Sequences ◽  
Biological Phosphorus

A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T, was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1 ω7c and C18 : 1 ω7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G+C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9 %, respectively. Levels of DNA–DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chemotaxonomic data and molecular characteristics, strain EMB325T is considered to represent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is EMB325T (=KCTC 12608T=DSM 17964T).

scholarly journals Sphingobacterium daejeonense sp. nov., isolated from a compost sample

2006 ◽  
Vol 56 (9) ◽  
pp. 2031-2036 ◽  
Author(s):  
Kyoung-Ho Kim ◽  
Leonid N. Ten ◽  
Qing-Mei Liu ◽  
Wan-Taek Im ◽  
Sung-Taik Lee
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
The Family

A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated TR6-04T, was isolated from compost and characterized taxonomically by using a polyphasic approach. The organism grew optimally at 30 °C and at pH 6.5–7.0. The isolate was positive for catalase and oxidase tests but negative for gelatinase, indole and H2S production. Comparative 16S rRNA gene sequence analysis showed that strain TR6-04T fell within the radiation of the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii ATCC 33299T (96.7 % sequence similarity); the similarity to sequences of other species within the family Sphingobacteriaceae was less than 92.0 %. The G+C content of the genomic DNA was 38.7 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c). These chemotaxonomic data supported the affiliation of strain TR6-04T to the genus Sphingobacterium. However, on the basis of its phenotypic properties and phylogenetic distinctiveness, strain TR6-04T (=KCTC 12579T=LMG 23402T=CCUG 52468T) should be classified as the type strain of a novel species, for which the name Sphingobacterium daejeonense sp. nov. is proposed.

scholarly journals Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood

2007 ◽  
Vol 57 (3) ◽  
pp. 633-638 ◽  
Author(s):  
Zubair Aslam ◽  
Ju Hyoung Lim ◽  
Wan-Taek Im ◽  
Muhammad Yasir ◽  
Young Ryun Chung ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Gene Sequence Analysis ◽  
The Family

A novel, moderately halophilic, Gram-positive coccus, designated strain S2R53-5T, was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. Strain S2R53-5T grew in the presence of 0.5–15 % (w/v) NaCl and at pH 6.5–11.0, with optimum growth at 5 % (w/v) NaCl and pH 7.0. The temperature range for growth was 20.0–30.0 °C, with an optimum temperature of 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2R53-5T belongs to the family Staphylococcaceae and was most closely related to Salinicoccus roseus DSM 5351T (96.8 % gene sequence similarity), Salinicoccus hispanicus DSM 5352T (96.1 %), Salinicoccus alkaliphilus T8T (95.2 %) and Jeotgalicoccus halotolerans YKJ-101T (95.1 %). The genomic DNA G+C content was 47.0 mol%, which is in the range of 46–51 mol% that is characteristic for the genus Salinicoccus. Levels of DNA–DNA relatedness between strain S2R53-5T and S. roseus DSM 5351T, S. hispanicus DSM 5352T and S. alkaliphilus KCTC 13928T were 32.2, 15.4 and 4.6 %, respectively. Chemotaxonomic data (major menaquinone, MK-6; major fatty acids, iso-C15 : 0 and anteiso-C15 : 0; cell-wall murein type, Lys and Gly) and 16S rRNA gene sequence analysis supported the affiliation of strain S2R53-5T with the genus Salinicoccus. The combined evidence from the low DNA–DNA relatedness, physiological, biochemical and other genotypic data indicate that strain S2R53-5T clearly represents a novel species of the genus Salinicoccus, for which the name Salinicoccus jeotgali sp. nov. is proposed. The type strain is S2R53-5T (=KCTC 13030T=LMG 23640T).

pH: Keyfactor in the Biological Phosphorus Removal Process

1994 ◽  
Vol 29 (7) ◽  
pp. 71-74 ◽  
Author(s):  
G. J. F. Smolders ◽  
M. C. M. van Loosdrecht ◽  
J. J. Heijnen
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Glycogen Metabolism ◽  
Activated Sludge Process ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Biochemical Mechanisms ◽  
Biological Phosphorus

Experiments have been performed, using a sequencing batch reactor, to examine the effect of pH on biological phosphorus removal in the activated sludge process. The results, which indicate that glycogen metabolism occurs during anaerobic conditions, are useful in elucidating the biochemical mechanisms involved in phosphorus-removal, and have potential implications for systems such as Phostrip.

scholarly journals Chryseobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a Rhus vernicifera-cultivated field

2011 ◽  
Vol 61 (6) ◽  
pp. 1430-1435 ◽  
Author(s):  
Wan-Taek Im ◽  
Jung-Eun Yang ◽  
Se-Young Kim ◽  
Tae-Hoo Yi
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Compound K ◽  
Active Components ◽  
The Family ◽  
Rhus Vernicifera ◽  
Sequence Similarities

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated THG 15T, was isolated from soil of a field cultivated with Rhus vernicifera in Okcheon province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain THG 15T grew optimally at 25–30 °C and at pH 7 in the absence of NaCl on nutrient agar. Strain THG 15T displayed β-glucosidase (aesculinase) activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) into compound K via Rd and F2. On the basis of 16S rRNA gene sequence similarities, strain THG 15T was shown to belong to the family Flavobacteriaceae and was most closely related to Chryseobacterium soldanellicola PSD1-4T (97.7 % sequence similarity), Chryseobacterium soli JS6-6T (97.5 %) and Chryseobacterium indoltheticum LMG 4025T (97.3 %). The G+C content of the genomic DNA was 35.7 mol%. The major menaquinone was MK-6 and the major fatty acids were iso-C15 : 0 (50.3 %), iso-C17 : 0 3-OH (21.9 %), summed feature 4 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH; 9.5 %) and iso-C17 : 1ω9c (9.3 %). DNA sequence analysis and chemotaxonomic data supported the affiliation of strain THG 15T to the genus Chryseobacterium. DNA–DNA relatedness values between strain THG 15T and its closest phylogenetic neighbours were <15 %. Strain THG 15T could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium. The isolate therefore represents a novel species, for which the name Chryseobacterium ginsenosidimutans sp. nov. is proposed. The type strain is THG 15T ( = KACC 14527T  = JCM 16719T).

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