scholarly journals Lechevalieria xinjiangensis sp. nov., a novel actinomycete isolated from radiation-polluted soil in China

2007 ◽  
Vol 57 (12) ◽  
pp. 2819-2822 ◽  
Author(s):  
Wei Wang ◽  
Zhidong Zhang ◽  
Qiyong Tang ◽  
Jun Mao ◽  
Dong Wei ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Polluted Soil ◽  
Western China ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Phyletic Line ◽  
North Western

A novel actinomycete was isolated from radiation-polluted soil collected from Xinjiang in north-western China. The isolate, strain R24T, was found to have chemical and morphological properties associated with members of the genus Lechevalieria. An almost-complete 16S rRNA gene sequence of the isolate was aligned with corresponding sequences from representatives of the genus Lechevalieria and related taxa, using three tree-making algorithms, and was shown to form a distinct phyletic line in the Lechevalieria phylogenetic tree. DNA–DNA relatedness and phenotypic data served to distinguish strain R24T from members of the three Lechevalieria species with validly published names. The genotypic and phenotypic data show that the isolate should be classified as a novel species within the genus Lechevalieria. The name proposed for this taxon is Lechevalieria xinjiangensis sp. nov. The type strain is R24T (=CGMCC 4.3525T=DSM 45081T).

scholarly journals Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench

2006 ◽  
Vol 56 (5) ◽  
pp. 1123-1126 ◽  
Author(s):  
Wasu Pathom-aree ◽  
Yuichi Nogi ◽  
Iain C. Sutcliffe ◽  
Alan C. Ward ◽  
Koki Horikoshi ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Tree ◽  
Taxonomic Status ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Phenotypic Data ◽  
Mariana Trench ◽  
Phyletic Line

The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA–DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.

scholarly journals Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea

2006 ◽  
Vol 56 (7) ◽  
pp. 1645-1649 ◽  
Author(s):  
Byung-Yong Kim ◽  
Hang-Yeon Weon ◽  
Sylvie Cousin ◽  
Seung-Hee Yoo ◽  
Soon-Wo Kwon ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Bacterial Strains ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
Greenhouse Soils ◽  
Sequence Similarities

Two yellow-pigmented, Gram-negative, rod-shaped bacterial strains, GH1-10T and GH29-5T, were isolated from greenhouse soils in Korea. 16S rRNA gene sequence analysis indicated that these strains were related to members of the genus Flavobacterium. Strain GH1-10T was most closely related to Flavobacterium psychrolimnae and Flavobacterium denitrificans, with sequence similarities of 95.9 and 95.2 %, respectively. Strain GH29-5T was most closely related to ‘Flavobacterium saliodium’, F. denitrificans and Flavobacterium frigoris, with sequence similarities of 94.3, 92.5 and 92.5 %, respectively. The major cellular fatty acids of GH1-10T were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c) and iso-C17 : 0 3-OH, and those of GH29-5T were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. Both strains contained menaquinone with six isoprene units (MK-6) as the sole quinone. The DNA G+C contents of GH1-10T and GH29-5T were 35 and 39 mol%, respectively. Based on the phylogenetic and phenotypic data presented, it is concluded that the two bacteria represent two separate novel species of the genus Flavobacterium. The names proposed to accommodate these organisms are Flavobacterium daejeonense sp. nov., with type strain GH1-10T (=KACC 11422T=DSM 17708T), and Flavobacterium suncheonense sp. nov., with type strain GH29-5T (=KACC 11423T=DSM 17707T).

scholarly journals Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil

2004 ◽  
Vol 54 (2) ◽  
pp. 599-603 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Luis A. Maldonado ◽  
Hamid Amir ◽  
René Pineau ◽  
Michael Goodfellow
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Sequence Data ◽  
Morphological Properties ◽  
Nocardia Asteroides ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Ultramafic Soil

The taxonomic position of an actinomycete isolated from a hypermagnesian ultramafic soil was examined using a polyphasic approach. The strain, designated SBHR OA6T, was shown to have chemical and morphological properties typical of members of the genus Nocardia. The organism was most closely associated with Nocardia asteroides using 16S rRNA gene sequence data. It showed a distinctive set of phenotypic properties that distinguished it from representatives of all species with validly published names classified in the genus Nocardia. The combined genotypic and phenotypic data show that strain SBHR OA6T (=DSM 44717T=NCIMB 13955T) merits description as the type strain of a novel Nocardia species, Nocardia neocaledoniensis sp. nov.

scholarly journals Amycolatopsis nigrescens sp. nov., an actinomycete isolated from a Roman catacomb

2007 ◽  
Vol 57 (3) ◽  
pp. 513-519 ◽  
Author(s):  
Ingrid Groth ◽  
Geok Yuan Annie Tan ◽  
Juan M. González ◽  
Leonila Laiz ◽  
Marc René Carlsohn ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Taxonomic Status ◽  
Maximum Parsimony Tree ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Parsimony Tree ◽  
High Bootstrap

The taxonomic status of two actinomycetes isolated from the wall of a hypogean Roman catacomb was established based on a polyphasic investigation. The organisms were found to have chemical and morphological markers typical of members of the genus Amycolatopsis. They also shared a range of chemical, molecular and phenotypic markers which served to separate them from representatives of recognized Amycolatopsis species. The new isolates formed a branch in the Amycolatopsis 16S rRNA gene sequence tree with Amycolatopsis minnesotensis NRRL B-24435T, but this association was not supported by a particularly high bootstrap value or by the product of the maximum-parsimony tree-making algorithm. The organisms were distinguished readily from closely related Amycolatopsis species based on a combination of phenotypic properties and from all Amycolatopsis strains by their characteristic menaquinone profiles, in which tetra-hydrogenated menaquinones with 11 isoprene units predominated. The combined genotypic and phenotypic data indicate that the isolates merit recognition as representing a novel species of the genus Amycolatopsis. The name proposed for this novel species is Amycolatopsis nigrescens sp. nov., with type strain CSC17Ta-90T (=HKI 0330T=DSM 44992T=NRRL B-24473T).

scholarly journals Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall

2007 ◽  
Vol 57 (9) ◽  
pp. 1995-1998 ◽  
Author(s):  
Qiang Gu ◽  
Wen Zheng ◽  
Ying Huang
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Physiological Properties ◽  
Phyletic Line ◽  
The 16S Rrna Gene ◽  
Chinese Medicinal Plant

An actinomycete, designated strain E71T, was isolated from the stem of Sambucus adnata Wall, a Chinese medicinal plant, and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence showed that the organism was a member of the genus Glycomyces, and formed a distinct phyletic line distantly related to recognized species of the genus Glycomyces. Morphological and chemotaxonomic data supported the affiliation of strain E71T to the genus Glycomyces. A number of physiological properties and a unique menaquinone profile allowed differentiation of the strain from related Glycomyces species. It is therefore proposed that strain E71T represents a novel species of the genus Glycomyces, for which the name Glycomyces sambucus sp. nov. is proposed. The type strain is E71T (=CGMCC 4.3147T=DSM 45047T).

scholarly journals Amycolatopsis plumensis sp. nov., a novel bioactive actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil

2005 ◽  
Vol 55 (5) ◽  
pp. 2057-2061 ◽  
Author(s):  
Danielle Saintpierre-Bonaccio ◽  
Hamid Amir ◽  
René Pineau ◽  
G. Y. Annie Tan ◽  
Michael Goodfellow
Keyword(s):  
Gene Sequence ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Properties ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Ultramafic Soil ◽  
Type Strains

The taxonomic position of an actinomycete isolated from a brown hypermagnesian ultramafic soil was examined using a polyphasic approach. The organism, which was designated SBHS Strp1T, was found to have chemical and morphological properties typical of Amycolatopsis strains. It was most closely associated with Amycolatopsis kentuckyensis, Amycolatopsis lexingtonensis, Amycolatopsis rifamycinica, Amycolatopsis pretoriensis and Amycolatopsis tolypomycina on the basis of 16S rRNA gene sequence data, and showed a unique pattern of phenotypic properties that distinguished it from the type strains of these taxa. The combined genotypic and phenotypic data show that the organism merits description as a novel species of Amycolatopsis. The name proposed for the novel species is Amycolatopsis plumensis sp. nov.; the type strain is SBHS Strp1T (=DSM 44776T=NRRL B-24324T).

scholarly journals Flaviflexus salsibiostraticola sp. nov., an actinobacterium isolated from a biofilm reactor

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3293-3296 ◽  
Author(s):  
Long Jin ◽  
So-Ra Ko ◽  
Hyung-Gwan Lee ◽  
Byung-Hyuk Kim ◽  
Hee-Sik Kim ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Biofilm Reactor ◽  
Rrna Gene ◽  
Gram Stain ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type

A Gram-stain-positive, aerobic, non-motile, non-spore-forming, cocci-shaped actinobacterium, designated strain EBR4-1-2T, was isolated from a biofilm reactor in Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class Actinobacteria , and was related most closely to Flaviflexus huanghaiensis H5T, showing 98.9 % similarity. Cells of strain EBR4-1-2T formed yellow colonies on R2A agar, contained MK-9(H4) as the predominant menaquinone, and included C18 : 1ω9c, C16 : 0, C16 : 1ω9c and C14 : 0 as the major fatty acids. The cell-wall peptidoglycan type was A5α (l-Lys–l-Ala–l-Lys–d-Glu). The G+C content of the genomic DNA of strain EBR4-1-2T was 65.6 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain EBR4-1-2T represents a novel species of the genus Flaviflexus , for which the name Flaviflexus salsibiostraticola sp. nov. is proposed. The type strain is EBR4-1-2T ( = KCTC 33148T = JCM 19016T).

scholarly journals Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov.

2004 ◽  
Vol 54 (1) ◽  
pp. 211-214 ◽  
Author(s):  
Seung Bum Kim ◽  
Chi Nam Seong ◽  
Soo Jin Jeon ◽  
Kyung Sook Bae ◽  
Michael Goodfellow
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Sequence Data ◽  
Novel Species ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
Rrna Gene Sequence ◽  
Phyletic Line ◽  
The Subject ◽  
Diverse Groups

Acidophilic actinomycete strains that represent the two major neutrotolerant clusters defined by numerical taxonomy (Seong, 1992) were the subject of a polyphasic taxonomic study. The centrotypes of each cluster, designated as strain JL164 (=KCTC 9924) of cluster 21 and strain CN732T (=KCTC 9926T=IMSNU 50114T=NRRL B-24245T) of cluster 13, were assigned initially to the genus Streptomyces on the basis of morphological and chemotaxonomic characteristics; this assignation was confirmed by 16S rRNA gene sequence data. Strain CN732T formed a distinct phyletic line within the Streptomyces tree, whereas strain JL164 was related closely to the type strain of Streptomyces mirabilis. It is evident from the present and previous studies that neutrotolerant acidophilic actinomycetes comprise taxonomically diverse groups within the variation encompassed by the genus Streptomyces. It is also apparent that strain CN732T and other members of cluster 13 merit recognition as a novel species, for which the name Streptomyces yeochonensis sp. nov. is proposed.

scholarly journals Streptomyces samsunensis sp. nov., a member of the Streptomyces violaceusniger clade isolated from the rhizosphere of Robinia pseudoacacia

2011 ◽  
Vol 61 (6) ◽  
pp. 1309-1314 ◽  
Author(s):  
Anıl Sazak ◽  
Nevzat Şahin ◽  
Kıymet Güven ◽  
Kamil Işık ◽  
Michael Goodfellow
Keyword(s):  
Gene Sequence ◽  
Sequence Data ◽  
Novel Species ◽  
Robinia Pseudoacacia ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Streptomyces Violaceusniger ◽  
Streptomyces Violaceusniger Clade ◽  
Streptomyces Malaysiensis

The taxonomic position of a Streptomyces isolate, strain M1463T, recovered from the rhizosphere of Robinia pseudoacacia was established in a polyphasic study. The organism had chemical and morphological markers that were consistent with its classification in the Streptomyces violaceusniger clade. This assignment was confirmed by 16S rRNA gene sequence data, which also showed that the strain formed a distinct subclade together with Streptomyces malaysiensis DSM 41697T. However, the two strains were readily distinguished on the basis of DNA relatedness and phenotypic data. The combined genotypic and phenotypic data show that strain M1463T should be recognized as a representative of a novel species in the Streptomyces violaceusniger clade, for which the name Streptomyces samsunensis sp. nov. is proposed. The type strain of S. samsunensis is M1463T ( = DSM 42010T = NRRL B-24803T).

scholarly journals Amycolatopsis thailandensis sp. nov., a poly(l-lactic acid)-degrading actinomycete, isolated from soil

2011 ◽  
Vol 61 (4) ◽  
pp. 839-843 ◽  
Author(s):  
Atchareeya Chomchoei ◽  
Wasu Pathom-aree ◽  
Akira Yokota ◽  
Chartchai Kanongnuch ◽  
Saisamorn Lumyong
Keyword(s):  
Lactic Acid ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Phenotypic Data ◽  
Phyletic Line

A novel actinomycete that was capable of degrading poly(l-lactic acid), strain CMU-PLA07T, was isolated from soil in northern Thailand. Strain CMU-PLA07T had biochemical, chemotaxonomic, morphological and physiological properties that were consistent with its classification in the genus Amycolatopsis. 16S rRNA gene sequence analysis showed that the isolate formed a phyletic line within the genus Amycolatopsis. Strain CMU-PLA07T was most similar to Amycolatopsis coloradensis IMSNU 22096T (99.5 % 16S rRNA gene sequence similarity) and Amycolatopsis alba DSM 44262T (99.4 %). However, strain CMU-PLA07T was distinguishable from the type strains of species of the genus Amycolatopsis on the basis of DNA–DNA relatedness and phenotypic data. Therefore, strain CMU-PLA07T is considered to represent a novel species of the genus Amycolatopsis, for which the name Amycolatopsis thailandensis sp. nov. is proposed. The type strain is CMU-PLA07T ( = JCM 16380T = BCC 38279T).

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