scholarly journals Hanseniaspora jakobsenii sp. nov., a yeast isolated from Bandji, a traditional palm wine of Borassus akeassii

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3576-3579 ◽  
Author(s):  
Labia Irène I. Ouoba ◽  
Dennis S. Nielsen ◽  
Amarachukwu Anyogu ◽  
Christine Kando ◽  
Bréhima Diawara ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
No Production ◽  
The Novel ◽  
Closely Related Species ◽  
Palm Wine ◽  
26S Rrna ◽  
Genotypic Characteristics

Investigation of the microbial diversity of Bandji, a traditional palm wine from Burkina Faso (West Africa) revealed the presence of two yeast isolates (YAV16 and YAV17T) with unusual phenotypic and genotypic characteristics. The isolates divide by bipolar budding with no production of ascospores. Phylogenetic analysis of concatenated sequences of the 26S rRNA gene D1/D2 and internal transcribed spacer (ITS) regions indicated that the novel species was most closely related to Kloeckera lindneri and Hanseniaspora valbyensis. The new isolates differed from K. lindneri NRRL Y-17531T and H. valbyensis CBS 479T by substitutions in the D1/D2 region of 12 and 16 nt respectively. The divergence in the ITS region from the closely related species was characterized by substitutions of 45–46 nt. Repetitive palindromic PCR (rep-PCR) profiles of YAV16 and YAV17T were also significantly different from those of K. lindneri MUCL 31146T ( = NRRL Y-17531T), H. valbyensis NCYC 17T ( = CBS 479T) and other species of the genus Hanseniaspora. Based on the results of the phenotypic and genotypic characterizations, it was concluded that the new isolates represent a novel species for which the name Hanseniaspora jakobsenii sp. nov. is proposed with YAV17T ( = CBS 12942T = DSM 26339T = NCYC 3828T; MycoBank number MB 805785) as the type strain.

scholarly journals Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1855-1859 ◽  
Author(s):  
Ana Raquel O. Santos ◽  
Elisa S. Faria ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Mangifera Indica ◽  
Large Subunit ◽  
Its Region ◽  
Methylotrophic Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Mango Leaves

Five strains of a novel methanol-assimilating yeast species were isolated from mango (Mangifera indica) leaves collected at the campus of the Federal University of Minas Gerais in Brazil. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the Ogataea clade and is related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogensis and Candida xyloterini (Ogataea clade). The novel species differs in the D1/D2 domains of the large subunit of the rRNA gene by 12 to 40 substitutions from these Ogataea species. The name Ogataea mangiferae sp. nov. is proposed for this novel species. The type strain of Ogataea mangiferae sp. nov. is UFMG-CM-Y253T ( = CBS 13492T). The Mycobank number is MB 811646.

Sympodiomycopsis yantaiensis sp. nov., a basidiomycetous yeast isolated from insect frass

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3501-3505 ◽  
Author(s):  
Liang Chen ◽  
Lin Zhang ◽  
Zhi-Hui Li ◽  
Feng-Li Hui
Keyword(s):  
Type Strain ◽  
Large Subunit ◽  
Its Region ◽  
Basidiomycetous Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Sophora Japonica ◽  
Closely Related Species ◽  
D2 Domain ◽  
Lsu Rrna Gene

Two strains (NYNU 121010T and NYNU 121032) of a novel basidiomycetous yeast species belonging to the genus Sympodiomycopsis were isolated from insect frass collected from trunks of a pagoda tree (Sophora japonica L.) in Yantai, Shandong province, east China. The sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the closest relatives were Sympodiomycopsis kandeliae FIRDI 007T, Sympodiomycopsis paphiopedili CBS 7429T and Sympodiomycopsis sp. S6A. The D1/D2 sequences of the novel strains differed by 12 nt substitutions (2 %) from the type strain of S. kandeliae, and by 13 nt substitutions (2.2 %) from the type strain of S. paphiopedili and from Sympodiomycopsis sp. S6A. The novel strains differed from closely related species by more than 4.6 % substitutions in the ITS region. The novel strains can also be distinguished from S. kandeliae and S. paphiopedili on the basis of a number of morphological and physiological characteristics and represent a novel species in the genus Sympodiomycopsis, for which the name Sympodiomycopsis yantaiensis sp. nov. is proposed. The type strain is NYNU 121010T ( = CICC 32998T = CBS 12813T). The Mycobank deposit number is MB 804119.

scholarly journals Candida halmiae sp. nov., Geotrichum ghanense sp. nov. and Candida awuaii sp. nov., isolated from Ghanaian cocoa fermentations

2010 ◽  
Vol 60 (6) ◽  
pp. 1460-1465 ◽  
Author(s):  
Dennis S. Nielsen ◽  
Mogens Jakobsen ◽  
Lene Jespersen
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Closely Related Species ◽  
Group A ◽  
Group B ◽  
26S Rrna ◽  
Sequence Similarities

During an investigation of the microbiology of Ghanaian cocoa fermentations, a number of yeast isolates with unusual pheno- and genotypic properties representing three possible novel species were isolated. Members of Group A divided by multilateral budding and ascospores were not produced. Group B strains produced true hyphae and ascospores were not produced. Group C representatives divided by budding and formed chains and star-like aggregates. Ascospores were not produced. Sequence analysis of the 26S rRNA gene (D1/D2 region) revealed that the Group A isolates were phylogenetically most closely related to Saturnispora mendoncae (gene sequence similarity 92.4 %), Saturnispora besseyi (88.8 %), Saturnispora saitoi (88.8 %) and Saturnispora ahearnii (88.3 %). Members of Group B were most closely related to representatives of the genera Dipodascus and Galactomyces and the asporogenous genus Geotrichum, but in all cases with 26S rRNA gene (D1/D2 region) similarities below 87 %. For Group C, the most closely related species were Candida rugopelliculosa (92.4 %), Pichia occidentalis (91.6 %) and Pichia exigua (91.9 %). The very low gene sequence similarities obtained for the three groups of isolates clearly indicated that they represented novel species. Repetitive Palindromic PCR (Rep-PCR) of the isolates and their closest phylogenetic relatives confirmed that the new isolates belonged to previously undescribed species. In conclusion, based on the genetic and phenotypic results, the new isolates were considered to represent three novel species, for which the names Candida halmiae (group A, type strain G3T=CBS 11009T=CCUG 56721T); Geotrichum ghanense (group B, type strain G6T=CBS 11010T=CCUG 56722T) and Candida awuaii (group C, type strain G15T=CBS 11011T=CCUG 56723T) are proposed.

Kwoniella shandongensis sp. nov., a basidiomycetous yeast isolated from soil and bark from an apple orchard

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2774-2777 ◽  
Author(s):  
Ru Chen ◽  
Yuan-Mao Jiang ◽  
Shao-Chong Wei ◽  
Qi-Ming Wang
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Apple Orchard ◽  
Basidiomycetous Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Yeast Strains ◽  
Its Regions ◽  
Pr China ◽  
26S Rrna

Four basidiomycetous yeast strains (Y13-1T, Y2-1, Y6-3 and Y8-2) were isolated from soil and bark collected from an apple orchard in Tai’an, Shandong province, PR China. Phylogenetic analysis based on 26S rRNA gene D1/D2 domains and ITS regions revealed that these novel strains were located in the Kwoniella clade in the class Tremellomycetes and were closely related to Cryptococcus cuniculi and Kwoniella heveanensis, but were clearly distinct from these species. Therefore, it is proposed that the new strains represent a novel species, Kwoniella shandongensis sp. nov., with the type strain Y13-1T( = CGMCC 2.04458T = CBS 12478T). The MycoBank number for the novel species is MB 564868.

scholarly journals Bullera vrieseae sp. nov., a tremellaceous yeast species isolated from bromeliads

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2466-2471 ◽  
Author(s):  
Melissa Fontes Landell ◽  
Luciana R. Brandão ◽  
Silvana V. B. Safar ◽  
Fatima C. O. Gomes ◽  
Ciro R. Félix ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
The Novel ◽  
D2 Domain ◽  
Lsu Rrna Gene

Two independent surveys of yeasts associated with different bromeliads in different Brazilian regions led to the proposal of a novel yeast species, Bullera vrieseae sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences in the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested affinity to a phylogenetic lineage that includes Bullera miyagiana and Bullera sakaeratica. Six isolates of the novel species were obtained from different bromeliads and regions in Brazil. Sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene showed that the novel species differs from B. miyagiana and B. sakaeratica by 85 and 64 nt substitutions, respectively and by more than 75 nt substitutions in the ITS region. Phenotypically, Bullera vrieseae sp. nov. can be distinguished from both species based on the assimilation of meso-erythritol, which was negative for B. vrieseae sp. nov. but positive for the others, assimilation of d-glucosamine, which was positive for B. vrieseae sp. nov. but negative for B. miyagiana and of l-sorbose, which was negative for B. vrieseae sp. nov. but positive for B. sakaeratica. The novel species Bullera vrieseae sp. nov. is proposed to accommodate these isolates. The type strain of Bullera vrieseae sp. nov. is UFMG-CM-Y379T (BRO443T; ex-type CBS 13870T).

scholarly journals Leucosporidium drummii sp. nov., a member of the Microbotryomycetes isolated from soil

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 728-734 ◽  
Author(s):  
A. M. Yurkov ◽  
A. M. Schäfer ◽  
D. Begerow
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Rrna Gene ◽  
Grassland Soil ◽  
Core Group ◽  
The Core ◽  
26S Rrna ◽  
Phenotypic Tests

Two strains of a novel teleomorphic basidiomycete were isolated from grassland soil. Standard phenotypic tests and phylogenetic analyses of 26S rRNA gene (D1/D2 domains) and ITS region sequences showed that the species belongs to the core group of the genus Leucosporidium. A novel species, Leucosporidium drummii sp. nov., is proposed to accommodate the two strains, with SEG-3-2-AY220T ( = CBS 11562T = MUCL 52878T) as the type strain. In addition, phylogenetic analysis revealed great genetic variability in the Leucosporidium scottii complex.

scholarly journals Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004

2007 ◽  
Vol 57 (9) ◽  
pp. 1988-1994 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Kae Kyoung Kwon ◽  
Dong Sung Shin ◽  
Xuseong Luo ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Genotypic Characteristics ◽  
Sequence Similarities

A taxonomic study of a novel marine, heterotrophic, non-gliding, halotolerant and light-pink-pigmented bacterium was carried out using a polyphasic approach. 16S rRNA gene sequence analysis revealed that strain KMM 6241T is a member of the phylum Bacteroidetes. Strain KMM 6241T formed a cluster with the genera Algoriphagus, Chimaereicella and Hongiella with sequence similarities of 94.0–98.2 %. Hongiella ornithinivorans was the closest relative of the novel isolate. Comparative analysis of phenotypic, chemotaxonomic and genotypic characteristics of strain KMM 6241T and representatives of the genera Algoriphagus, Chimaereicella and Hongiella revealed many similar features. Consequently, phylogenetic evidence supported by phenotypic and genotypic similarities support the transfer of members of the genera Chimaereicella and Hongiella to the genus Algoriphagus and the establishment of a novel species, Algoriphagus vanfongensis sp. nov., with strain KMM 6241T (=DSM 17529T=KCTC 12716T) as the type strain.

scholarly journals Cryptococcus thermophilus sp. nov., isolated from cassava sourdough

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1715-1720 ◽  
Author(s):  
Stephanie A. Vogelmann ◽  
Sandra Chaves ◽  
Christian Hertel
Keyword(s):  
Sodium Nitrite ◽  
Type Strain ◽  
Novel Species ◽  
Soluble Starch ◽  
Rrna Gene ◽  
The Novel ◽  
Cassava Flour ◽  
D2 Domain ◽  
Anamorphic Yeast ◽  
26S Rrna

A novel anamorphic yeast, strain LTH 6662T, was isolated from cassava sourdough. The isolate supposedly originated from cassava flour or was a contaminant thereof. Sequencing of the D1/D2 domain of the 26S rRNA gene indicated that strain LTH 6662T represents a novel species. Its closest relatives were members of the Cryptococcus humicola complex. The novel strain had several physiological characteristics that differed from those of related species: the ability to assimilate raffinose and cadaverine; the inability to assimilate soluble starch, xylitol, galactitol, butane-2,3-diol, sodium nitrite and lysine; the ability to grow without vitamins and at 42 °C; and the inability to produce starch-like substances. Its major ubiquinone was Q-10. In addition, buds were formed on small neck-like structures. In liquid medium, green or blue fluorescent substances were produced. The name Cryptococcus thermophilus sp. nov. is proposed, with LTH 6662T ( = DSM 19443T = CBS 10687T) as the type strain.

Nakazawaea siamensis f.a., sp. nov., a yeast species isolated from phylloplane

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 266-270 ◽  
Author(s):  
Rungluk Kaewwichian ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
External Surface ◽  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Strain DMKU-RK467T, representing a novel yeast species, was isolated from the external surface of sugar cane leaves collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK467T was assigned to a novel species of the genus Nakazawaea. The novel species was related most closely to the type strain of Candida wickerhamii but they differed by 1.9 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and by 5.2 % nucleotide substitutions in the ITS region. The name Nakazawaea siamensis f.a., sp. nov. is proposed (type strain DMKU-RK467T = BCC 50734T = NBRC 108903T = CBS 12569T).

scholarly journals Pontibacter akesuensis sp. nov., isolated from a desert soil in China

2007 ◽  
Vol 57 (2) ◽  
pp. 321-325 ◽  
Author(s):  
Yu Zhou ◽  
Xu Wang ◽  
Huan Liu ◽  
Ke-Yun Zhang ◽  
Yu-Qin Zhang ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Desert Soil ◽  
Rrna Gene ◽  
Taxonomic Study ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Gram Negative ◽  
Genotypic Characteristics

A Gram-negative, non-motile, rod-shaped bacterium, designated strain AKS 1T, was isolated from a desert soil sample collected from Akesu, XinJiang Province, China. A taxonomic study, including phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics, was performed on the novel isolate. The predominant menaquinone of strain AKS 1T was MK-7. The major fatty acids included i-C15 : 0, ai-C17 : 1 B/i-C17 : 1 I and i-C17 : 0 3-OH. The G+C content of the DNA was 51.4 mol%. Based on the results of phenotypic and genotypic characteristics, strain AKS 1T should be assigned as representing a novel species of the genus Pontibacter, for which the name Pontibacter akesuensis is proposed. The type strain is AKS 1T (=KCTC 12758T=CCTCC AB 206086T).

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