scholarly journals Adhaeribacter arboris sp. nov., and Adhaeribacter pallidiroseus sp. nov., novel bacteria isolated from birch tree (Betula platyphylla)

2020 ◽  
Vol 70 (12) ◽  
pp. 6195-6202 ◽  
Author(s):  
Heeyoung Kang ◽  
Haneul Kim ◽  
Inseong Cha ◽  
Kiseong Joh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
Content Type ◽  
Link Type ◽  
Birch Tree ◽  
The Family

Two Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HMF7605T and HMF7616T, were isolated from birch tree, in Yong-in, Republic of Korea. Strains HMF7605T and HMF7616T exhibited the highest 16S rRNA gene sequence similarities of 95.9 and 97.5 % to Adhaeribacter swui 17mud1-7T, 97.9 % between themselves. The values of average nucleotide identity and in silico DNA–DNA hybridization between strains HMF7605T and HMF7616T were 77.6 and 22.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus Adhaeribacter within the family Hymenobacteraceae . The predominant fatty acids of both strains were iso-C15 : 0, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c), C16 : 1  ω5c and summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B). The both strains contained menaquinone-7 as the only isoprenoid quinone. The major polar lipid profiles of the two strains were similar with phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified glycolipid and three unidentified polar lipids. The DNA G+C contents of strains HMF7605T and HMF7616T were 42.0 and 42.8 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Adhaeribacter arboris sp. nov. and Adhaeribacter pallidiroseus sp. nov. are proposed. The type strains are HMF7605T (=KCTC 62465T=NBRC 113228T) and HMF7616T (=KCTC 62466T=NBRC 113229T), respectively.

Flavobacterium solisilvae sp. nov. and Flavobacterium silvaticum sp. nov., isolated from forest soil

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Hye Su Jung ◽  
Byung Hee Chun ◽  
Hyung Min Kim ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Forest Soil ◽  
Type Species ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

Two Gram-stain-negative, yellow-pigmented and strictly aerobic bacteria, designated strains SE-s27T and SE-s28T, were isolated from forest soil. Both strains were non-motile rods that were catalase-positive and oxidase-negative and grew optimally at 25–30 °C, pH 8.0 and with 0 % (w/v) NaCl. Strain SE-s28T produced flexirubin-type pigments, but strain SE-s27T did not produce them. Both strains contained menaquinone-6 as the sole respiratory quinone and phosphatidylethanolamine as a major polar lipid. As the major cellular fatty acids (>10 %), SE-s27T contained iso-C15 : 1 and iso-C15 : 1G, whereas SE-s28T contained iso-C15 : 0 and summed feature 3 comprising C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH. The DNA G+C contents of strains SE-s27T and SE-s28T were 33.1 and 44.3 mol%, respectively. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that SE-s27T and SE-s28T formed respective distinct phylogenetic lineages within the genus Flavobacterium . Strains SE-s27T and SE-s28T were most closely related to Flavobacterium macrobrachii an-8T and Flavobacterium piscinae ICH-30T with 98.0 and 94.5 % 16S rRNA gene sequence similarities, respectively. In conclusion, strains SE-s27T and SE-s28T represent novel species of the genus Flavobacterium , for which the names Flavobacterium solisilvae sp. nov. and Flavobacterium silvaticum sp. nov. are proposed. The type strains of F. solisilvae and F. silvaticum are SE-s27T (=KACC 18802T=JCM 31544T) and SE-s28T (=KACC 18803T=JCM 31545T), respectively.

scholarly journals Mangroviflexus xiamenensis gen. nov., sp. nov., a member of the family Marinilabiliaceae isolated from mangrove sediment

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1819-1824 ◽  
Author(s):  
Chao Zhao ◽  
Zhaoming Gao ◽  
Qiwei Qin ◽  
Lingwei Ruan
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Analyses ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Fermentation Products ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-negative, obligately anaerobic, non-spore-forming, long rod-shaped bacterium strain P2T was isolated from the offshore mangrove sediment of the South China Sea. Growth was observed at between 22 and 39 °C, with an optimum at 35 °C. The pH range for growth was 5.0–8.5, with an optimum around pH 7.0–7.5. Salt tolerance was determined between 0.2 and 3.5 % (w/v), optimum at 0.5–1.0 %. Catalase and oxidase activities were negative. Strain P2T utilized cysteine, lactate, pyruvate, yeast extract or H2/CO2+acetate as electron donors, and sulfate or sulfite as electron acceptors. Metabolism was strictly fermentative. The main organic fermentation products were propionate, acetate and succinate. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain P2T formed a distinct evolutionary lineage within the family Marinilabiliaceae . Strain P2T was most closely related to members of the genera Alkaliflexus (92.0 % 16S rRNA gene sequence similarity), Marinilabilia (91.7 %) and Anaerophaga (89.9 %) of the family Marinilabiliaceae . The DNA G+C content of the novel strain was 44.2±1.0 mol%. The dominant fatty acids of strain P2T were iso-C15 : 0 (33.5 %), anteiso-C15 : 0 (18.9 %), C16 : 0 (5.4 %), C16 : 0 3-OH (7.7 %) and iso-C17 : 0 3-OH (13.3 %). The respiratory quinone was menaquinone 7 (100 % of total quinone) and the major polar lipid was phosphatidylethanolamine. Strain P2T was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of phylogenetic, phenotypic and physiological evidence, a novel genus, Mangroviflexus, is proposed to harbour strain P2T ( = CGMCC 1.5167T = DSM 24214T) which is described as the type strain of a novel species, Mangroviflexus xiamenensis gen. nov., sp. nov.

scholarly journals Kangsaoukella pontilimi gen. nov., sp. nov., a new member of the family Rhodobacteraceae isolated from a tidal mudflat

2020 ◽  
Vol 70 (10) ◽  
pp. 5235-5242 ◽  
Author(s):  
Soon Dong Lee ◽  
Hanna Choe ◽  
Ji-Sun Kim ◽  
In Seop Kim
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Draft Genome ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Gene Similarity

A strictly aerobic, Gram-stain-negative, non-motile, ovoid- and rod-shaped bacterium, designated strain GH1-50T, was isolated from a tidal mudflat sample collected from Dongmak seashore on Gangwha Island, Republic of Korea. The organism showed growth at 20–40 °C (optimum, 30 °C), pH 7–8 (optimum, pH 7) and 2–6  % (w/v) NaCl (optimum, 5 %). The pufLM genes were present but bacteriochlorophyll a was not detected. The major isoprenoid quinone was Q-10. The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified aminolipid and five unidentified lipids. The predominant cellular fatty acids were C18 : 1  ω7c, C18 : 1  ω7c 11-methyl and C18 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the isolate belonged to the family Rhodobacteraceae and was loosely associated with members of the recognized genera. The closest relative was the type strain of Pseudoruegeria marinistellae (96.8 % similarity) followed by Boseongicola aestuarii (96.4 %). Other members of the family shared 16S rRNA gene similarity values below 96.0 % to the novel isolate. The DNA G+C content calculated from the draft genome sequence was 64.0 %. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values between genome sequences of strain GH1-50T and all the type strains of the recognized taxa compared were <70.0, <84.1 and <20.5 %, respectively. Based on data obtained by a polyphasic approach, strain GH1-50T (=KCTC 72224T=NBRC 113929T) represents a novel species of a new genus in the family Rhodobacteraceae , for which the name Kangsaoukella pontilimi gen. nov., sp. nov. is proposed.

Mucilaginibacter aquatilis sp. nov., Mucilaginibacter arboris sp. nov., and Mucilaginibacter ginkgonis sp. nov., novel bacteria isolated from freshwater and tree bark

2021 ◽  
Author(s):  
Heeyoung Kang ◽  
Haneul Kim ◽  
Seokhyeon Bae ◽  
Kiseong Joh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Tree Bark ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

Three Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HME9299T, HMF7410T and HMF7856T, were isolated from freshwater and tree bark collected in Yong-in, Republic of Korea. Strains HME9299T, HMF7410T and HMF7856T exhibited the highest 16S rRNA gene sequence similarities of 97.2, 94.4 and 96.4 % to Mucilaginibacter daejeonensis Jip 10T, Mucilaginibacter terrae CCM 8645T and Mucilaginibacter phyllosphaerae PP-F2F-G21T, respectively. Among themselves, the values were 94.1–95.7 %. Phylogenetic analysis of the 16S rRNA gene sequences of the three isolates revealed that they belonged to the genus Mucilaginibacter within the family Sphingobacteriaceae . The predominant fatty acids of three strains were summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c) and iso-C15 : 0. Strain HME9299T contained a relatively large amount of C16 : 1  ω5c. The predominant respiratory quinone was menaquinone-7. The genome sizes of strains HME9299T, HMF7410T and HMF7856T were 4.33, 4.16 and 3.68 Mbp, respectively, and their DNA G+C contents were 41.6, 38.4 and 43.9 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, three novel species, Mucilaginibacter aquatilis sp. nov, Mucilaginibacter arboris sp. nov. and Mucilaginibacter ginkgonis sp. nov., are proposed. The type strains are HME9299T (=KCTC 42122T=DSM 29146T), HMF7410T (=KCTC 62464T=NBRC 113227T) and HMF7856T (=KCTC 72782T=NBRC 114275T), respectively.

scholarly journals Spongiibacterium flavum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the marine sponge Halichondria oshoro, and emended descriptions of the genera Croceitalea and Flagellimonas

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1158-1164 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Duck-Chul Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sponge ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, yellow-pigmented rod, designated A11T, was isolated from a marine sponge, Halichondria oshoro, collected on the coastline of Jeju Island, Republic of Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A11T was a member of the family Flavobacteriaceae . Its closest relatives were members of the genera Muricauda , Flagellimonas and Croceitalea (94.4–94.8 % 16S rRNA gene sequence similarity). The only polar lipid detected in strain A11T was phosphatidylethanolamine. The dominant fatty acids were iso-C15 : 0 (30.4 %), iso-C15 : 1 G (26.7 %), iso-C17 : 0 3-OH (12.4 %) and iso-C15 : 0 3-OH (7.3 %). The DNA G+C content of strain A11T was 41.7 mol% and its major respiratory quinone was MK-6. On the basis of combined data from phenotypic and phylogenetic analyses, strain A11T represents a novel genus and species within the family Flavobacteriaceae , for which the name Spongiibacterium flavum gen. nov., sp. nov. is proposed. The type strain of the type species is A11T ( = KCTC 22665T = DSM 22638T). Emended descriptions of the genera Croceitalea and Flagellimonas are also given.

scholarly journals Carideicomes alvinocaridis gen. nov., sp. nov., a marine bacterium isolated from shrimp gill in a hydrothermal field of Okinawa Trough

2020 ◽  
Vol 70 (3) ◽  
pp. 1777-1784 ◽  
Author(s):  
Yiting Wang ◽  
Lijun Liu ◽  
Min Yu ◽  
Shun Zhou ◽  
Tianyu Fu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Okinawa Trough ◽  
Hydrothermal Field ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-stain-negative, strictly aerobic, oval-shaped, non-motile bacterium with no flagella, designated strain SCR17T, was isolated from a shrimp gill habitat in Tangyin hydrothermal field of Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SCR17T formed a lineage within the family ‘ Rhodobacteraceae ’, and shared 16S rRNA gene sequence similarity of 93.2–96.2 % to the related genera Aquicoccus and Roseivivax . Strain SCR17T was able to grow with 0–14 % (w/v) NaCl (optimum, 9–10 %). The sole respiratory quinone was ubiquinone-10. The major polar lipids of strain SCR17T comprised phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unidentified aminolipid (AL), an unidentified phospholipid (PL) and an unidentified lipid (L). The predominant fatty acids (more than 10 % of the total fatty acids) were C18 : 1ω7c or/and C18 : 1ω6c, anteiso-C15 : 0, C16 : 0 and C19 : 0 cyclo ω8c . The genomic DNA G+C content of strain SCR17T was 67.7 mol%. Based on polyphasic taxonomic analyses, strain SCR17T is considered to represent a novel species in a new genus of the family ‘ Rhodobacteraceae ’, for which the name Carideicomes alvinocaridis gen. nov., sp. nov. is proposed. The type strain of Carideicomes alvinocaridis is SCR17T (=JCM 33426T=MCCC 1K03732T). The discovery of a novel host-associated bacterium in hydrothermal fields provides an opportunity for the study of host–bacterial symbiosis in extreme environments.

scholarly journals Frigidibacter albus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from lake water

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1199-1206 ◽  
Author(s):  
Ai-Hua Li ◽  
Yu-Guang Zhou
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Cold Water ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family

Three Gram-staining-negative, strictly aerobic, non-pigmented, non-motile, rod-shaped bacterial strains, SP32T ( = SLM-1T), SR68 ( = SLM-3) and SP95 ( = SLM-2), were isolated from two water samples of a cold-water lake in Xinjiang province, China. Growth was observed at 4–25 °C and pH 6.0–9.0, and optimum growth occurred at 18–20 °C and at pH 7.0–7.5. Phylogenetic analysis of 16S rRNA gene sequences revealed that these isolates belonged to the family Rhodobacteraceae , but formed an evolutionary lineage distinct from other species of this family with validly published names. Strain SP32T showed the highest 16S rRNA gene sequence similarity (96.7 %) to Rhodobacter veldkampii ATCC 35703T, and the similarity to members of the genera Defluviimonas , Haematobacter and Pseudorhodobacter was respectively 95.8–96.4, 96.0–96.1 and 95.3–96.1 %. The genomic DNA G+C content of strain SP32T was 67.6 mol%. The major fatty acids (>5 %) were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and11-methyl C18 : 1ω7c. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified glycolipid and one unidentified polar lipid were the main polar lipids. Ubiquinone 10 (Q-10) was the sole respiratory quinone. Strain SP32T did not produce photosynthetic pigments and did not contain the gene pufM, by which it differed from the phototrophic species of the family Rhodobacteraceae . Based on its distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SP32T represents a novel species in a novel genus within the family Rhodobacteraceae , for which we propose the name Frigidibacter albus gen. nov., sp. nov. The type strain of Frigidibacter albus is strain SP32T ( = SLM-1T = CGMCC 1.13995T = NBRC 109671T).

Flavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum , Flavobacterium beibuense and Flavobacterium rivuli

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3237-3242 ◽  
Author(s):  
Kun Dong ◽  
Biao Xu ◽  
Fengqiu Zhu ◽  
Gejiao Wang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A strictly aerobic, Gram-staining-negative, rod-shaped, non-motile, yellow-pigmented bacterial strain, designated BX12T, was isolated from soil collected from the peak area of Wudang Mountain in the city of Shiyan, Hubei province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain BX12T was most closely related to Flavobacterium subsaxonicum WB 4.1-42T (95.9 % 16S rRNA gene sequence similarity), followed by Flavobacterium beibuense F44-8T (95.6 %) and Flavobacterium rivuli WB 3.3-2T (94.1 %). The major fatty acids (≥5 %) of strain BX12T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, C16 : 0, iso-C17 : 0 3-OH, and C16 : 0 3-OH. The major polar lipid was phosphatidylethanolamine, and the major respiratory quinone was menaquinone-6. The genomic DNA G+C content was 43.9 mol%. On the basis of a high number of phenotypic differentiating properties and phylogenetic uniqueness, strain BX12T represents a novel species of the genus Flavobacterium for which the name Flavobacterium hauense sp. nov. is proposed. The type strain is BX12T ( = CCTCC AB 2012197T = KCTC 32147T). Emended descriptions of Flavobacterium subsaxonicum , Flavobacterium beibuense and Flavobacterium rivuli are also proposed.

Huanghella arctica gen. nov., sp. nov., a bacterium of the family Cytophagaceae isolated from Arctic tundra soil

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 696-702 ◽  
Author(s):  
Fan Jiang ◽  
Mengchen Xiao ◽  
Lu Chen ◽  
Wenjing Kan ◽  
Fang Peng ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Tundra Soil ◽  
Svalbard Archipelago ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel, strictly aerobic, red-pigmented, Gram-reaction-negative bacterium, designated strain R9-9T, was isolated from tundra soil collected near Ny-Ålesund, Svalbard Archipelago, Norway (78° N). The novel strain was subjected to a taxonomic study using a polyphasic approach. It grew optimally at 20–22 °C and at pH 7.0. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R9-9T represents a distinct phyletic line that reflects a novel generic status within the family Cytophagaceae . The novel strain showed relatively low 16S rRNA gene sequence similarities (<88.0 %) to members of established genera. Strain R9-9T contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, iso-C15 : 0 and C16 : 1ω5c as its major cellular fatty acids, phosphatidylethanolamine as its main polar lipid, and MK-7 as its major respiratory quinone. The genomic DNA G+C content was 56.1 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain R9-9T is considered to represent a novel species in a new genus in the family Cytophagaceae , for which the name Huanghella arctica gen. nov., sp. nov. is proposed. The type strain is R9-9T ( = CCTCC AB 2010418T = NRRL B-59750T).

scholarly journals Psychroflexus salarius sp. nov., isolated from Gomso salt pan

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3467-3472 ◽  
Author(s):  
Jeesun Chun ◽  
Ji Young Kang ◽  
Kwang Yeop Jahng
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Salt Pan

A taxonomic study was carried out on a novel bacterial strain, designated MIC1008T, which was isolated from the Gomso salt pan, located in Buan County, Korea. Cells were Gram-stain-negative, aerobic, non-motile and moderately halophilic rods, 0.2–0.4 µm wide and 0.7–1.2 µm long. The optimum temperature and pH for growth were 25 °C and pH 6.5–8.5, respectively. Catalase and oxidase activities were positive. Carotenoid pigments were produced. Comparison of 16S rRNA gene sequences indicated that the isolate belonged to the genus Psychroflexus , with Psychroflexus halocasei WCC 4520T as its closest relative, with a 16S rRNA gene sequence similarity of 95.4 %. The isolate contained iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, anteiso-C15 : 0 and iso-C15 : 0 3-OH as the major fatty acids and menaquinone MK-6 as the isoprenoid quinone. The major polar lipid profile of strain MIC1008T revealed the presence of phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of the isolate was 32.9 mol%. On the basis of the evidences presented, it is concluded that strain MIC1008T represents a novel species of the genus Psychroflexus within the family Flavobacteriaceae , for which the name Psychroflexus http://dx.doi.org/10.1601/nm.8182 salarius sp. nov. is proposed. The type strain is MIC1008T ( = KACC 17063T = DSM 25661T).

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