scholarly journals An outbreak of oropharyngeal tularaemia linked to natural spring water

2009 ◽  
Vol 58 (1) ◽  
pp. 112-116 ◽  
Author(s):  
A. Willke ◽  
M. Meric ◽  
R. Grunow ◽  
M. Sayan ◽  
E. J. Finke ◽  
...  

A tularaemia outbreak was investigated involving 188 suspected cases in the Kocaeli region of Turkey between December 2004 and April 2005. A case–control study comprising 135 laboratory-confirmed cases and 55 controls was undertaken to identify risk factors for the development of the outbreak and to evaluate laboratory diagnostic methods. Tularaemia was confirmed by a microagglutination test (MAT) titre of ≥1 : 160 in 90 of the patients. In MAT-negative sera, 23/44 (52 %) were positive by ELISA with Francisella tularensis LPS and 1/9 (11 %) by Western blotting with this antigen. A species-specific PCR was positive in 16/25 (64 %) throat swabs and 8/13 (62 %) lymph node aspirates. Multivariate analysis showed that drinking natural spring water was the leading risk factor for the development of tularaemia (P=0.0001, odds ratio 0.165, 95 % CI 0.790–0.346). The outbreak ceased after abandonment of the suspected natural water springs.

2007 ◽  
Vol 56 (5) ◽  
pp. 608-613 ◽  
Author(s):  
Oralak Serichantalergs ◽  
Nurul Amin Bhuiyan ◽  
Gopinath Balakrish Nair ◽  
Orapan Chivaratanond ◽  
Apichai Srijan ◽  
...  

Vibrio parahaemolyticus is a major cause of gastroenteritis worldwide. A total of 95 V. parahaemolyticus isolates belonging to 23 different serovars were identified in a case–control study of expatriates and Thai adults from 2001 to 2002 in Thailand. Fifty-two per cent of isolates (49/95) were resistant to ampicillin and sulfisoxazole, but all isolates were susceptible to ciprofloxacin and trimethoprim–sulfamethoxazole, two antibiotics commonly used to treat traveller’s diarrhoea. All isolates were positive for the species-specific toxR gene, and 91 and 5 were positive for the thermostable direct haemolysin (tdh) gene and the tdh-related (trh) gene, respectively. Sixty-five isolates were assigned to the pandemic group of V. parahaemolyticus by a group-specific PCR and the presence of the orf8 gene. The pandemic isolates belonged to three recognized serovars (O3 : K6, O1 : K25, O1 : KUT) and a new serovar, O3 : K46. This new serovar harboured pandemic traits. PFGE analysis revealed that all pandemic isolates including serovar O3 : K46 were closely related and clearly distinct from the non-pandemic isolates. In summary, three well-known serovars of pandemic V. parahaemolyticus isolates were identified as a major cause of diarrhoea in Thailand and a new V. parahaemolyticus isolate, serovar O3 : K46, with pandemic traits was detected.


2017 ◽  
Vol 23 (5-6) ◽  
pp. 299-313 ◽  
Author(s):  
Tiziano Boschetti ◽  
Lorenzo Toscani ◽  
Paola Iacumin ◽  
Enricomaria Selmo

2011 ◽  
Vol 16 (2) ◽  
pp. 1-5 ◽  
Author(s):  
Kwang-Rae Kim ◽  
Hae-Kyung Gil ◽  
Man-Ho Lee ◽  
Seok-Won Eom ◽  
Jai-Young Lee

2020 ◽  
Vol 08 (12) ◽  
pp. 200-210
Author(s):  
Eblin Sampah Georges ◽  
Konan Kouakou Séraphin ◽  
Ohou-Yao Marie-Jeanne Adélaïde ◽  
Mangoua Oi Mangoua Jules ◽  
Dibi Brou ◽  
...  

Author(s):  
Reza Najafipour ◽  
Abdolmabood Momeni ◽  
Farideh Yousefipour ◽  
Shaghayegh Mousavi ◽  
Sahar Moghbelinejad

Background: Post-transcriptional microRNAs (miRNAs) have an important pattern in the spermatogenesis process. Objective: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men. Materials and Methods: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samples. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts. Results: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%). Conclusion: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis. Key words: Spermatogenesis, miR-449, Expression, Epigenetic.


2012 ◽  
Vol 140-141 ◽  
pp. 12-20 ◽  
Author(s):  
G. Lutterodt ◽  
J.W.A. Foppen ◽  
S. Uhlenbrook

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