scholarly journals Underexpression of hsa-miR-449 family and their promoter hypermethylation in infertile men: A case-control study

2021 ◽  
Author(s):  
Reza Najafipour ◽  
Abdolmabood Momeni ◽  
Farideh Yousefipour ◽  
Shaghayegh Mousavi ◽  
Sahar Moghbelinejad
Keyword(s):  
Case Control Study ◽  
Promoter Hypermethylation ◽  
Case Control ◽  
Methylation Pattern ◽  
Specific Pcr ◽  
Infertile Men ◽  
Pcr Method ◽  
Defective Spermatogenesis ◽  
Control Study ◽  
Control Samples

Background: Post-transcriptional microRNAs (miRNAs) have an important pattern in the spermatogenesis process. Objective: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men. Materials and Methods: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samples. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts. Results: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%). Conclusion: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis. Key words: Spermatogenesis, miR-449, Expression, Epigenetic.

Association of decreased spermatozoa omega-3 fatty acid levels and increased oxidative DNA damage with varicocele in infertile men: a case control study

2016 ◽  
Vol 28 (5) ◽  
pp. 648 ◽  
Author(s):  
Li-Xin Tang ◽  
Dong-Juan Yuan ◽  
Qi-Ling Wang ◽  
Fang Jiang ◽  
Jian Guo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Case Control Study ◽  
Cell Structure ◽  
Case Control ◽  
Omega 3 ◽  
Infertile Men ◽  
Omega 6 ◽  
Control Study

Varicocele is commonly associated with male infertility because it impairs normal sperm morphology and activity. Polyunsaturated fatty acids (PUFA) are important determinants of sperm cell structure and function, but their relationship with varicocele remains unclear. The aim of the present study was to investigate the PUFA composition in spermatozoa of infertile men with varicocele and to evaluate the potential relationship between PUFA and varicocele. This case control study recruited 92 infertile men with varicocele, 99 infertile men without varicocele and 95 fertile male control subjects. Semen morphology and activity parameters were assessed and seminal plasma 8-hydroxy-2-deoxyguanosine (8-OHdG) content was determined by ELISA. Sperm concentrations of omega-3 and omega-6 fatty acids were measured by gas chromatography. Infertile men with varicocele had lower concentrations of omega-3 PUFA, higher omega-6 : omega-3 PUFA ratios and greater oxidative DNA damage in spermatozoa compared with infertile men without varicocele and normal subjects. The degree of varicocele and DNA damage was associated with decreased omega-3 PUFA concentrations and semen quality in infertile men with varicocele. The findings suggest that omega-3 PUFA deficiency could be implicated in varicocele-associated infertility, and highlight the need for intervention trials to test the usefulness of omega-3 supplementation in reducing sperm abnormalities in infertile men with varicocele.

REDUCED RISK OF PROSTATE CANCER IN INFERTILE MEN COMPARED TO MEN WITH PROVEN FERTILITY: A NESTED CASE- CONTROL STUDY

2008 ◽  
Vol 7 (3) ◽  
pp. 197
Author(s):  
Y. Ruhayel ◽  
A. Giwercman ◽  
P.A. Abrahamsson ◽  
L. Rylander ◽  
J. Manjer ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Case Control Study ◽  
Case Control ◽  
Infertile Men ◽  
Nested Case Control ◽  
Control Study ◽  
Reduced Risk

scholarly journals Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Ayad Palani ◽  
Ahmed Alahmar
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Case Control Study ◽  
Case Control ◽  
Semen Parameters ◽  
Infertile Men ◽  
Total Antioxidant ◽  
Control Study

Abstract Background Oxidative stress has been implicated in male infertility through decrease in sperm quality. However, men with normal semen parameters (normozoospermia) may be unable to fertilize their partners even when they have normal sperm function. Thus, they would be considered infertile. The purpose of this study was to investigate the role of oxidative stress in the pathogenesis of unexplained male infertility. Methods In this case–control study, infertile men with normozoospermia (n = 46) and fertile control group (n = 21) underwent seminal fluid analyses according to WHO 2010 criteria. Serum and seminal plasma levels of total antioxidant capacity (TAC), glutathione, malondialdehyde, uric acid and albumin were also measured using colorimetric methods. Results The level of total antioxidant capacity in both serum and seminal plasma was significantly lower in normozoospermic infertile men in comparison with fertile group (p < 0.0001). However, no significant differences were observed in serum and seminal plasma levels of glutathione, uric acid, albumin and malondialdehyde between infertile and fertile groups. Conclusion Low TAC level induces oxidative stress and consequently causes sperm dysfunction and male infertility. Estimation of TAC can be a useful tool in the diagnosis of male infertility. Antioxidant supplementation should be considered in the treatment of oxidative stress-induced male infertility.

scholarly journals Epidemiological and clinical aspects of respiratory pathologies during pregnancy and puerperality

2018 ◽  
Vol 7 (3) ◽  
pp. 829
Author(s):  
Kouamé Arthur Didier ◽  
Diomandé Fatoumata Alice ◽  
Kakou Charles ◽  
Alla Christian ◽  
Koffi Soh Victor ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
General Condition ◽  
Case Control Study ◽  
Case Control ◽  
Clinical Aspects ◽  
Age Group ◽  
Maternal Complications ◽  
Respiratory Pathology ◽  
Control Study ◽  
Control Samples

Background: The purpose of this study was to describe the characteristics of respiratory pathologies during pregnancy and postpartum.Methods: This was a case-control study over a 7-year period from January 2008 to December 2014 at CHU de COCODY. We compiled 86 cases of the PPH department hospitalized patients for pulmonary disease during pregnancy and for postpartum up to 42 days after delivery. The control samples were represented by those hospitalized in Obstetrics for a non-respiratory general condition during the same gravido puerperal period.Results: The age group of 20-29 years was the most affected in both groups with extremes ranging from 16 to 40 years (p=0.827). Respiratory pathology was common among housewives or unemployed women (p=0,001). Pauciparous and multiparous were the most affected (p=0.020). They had a medical history in 55.8% of cases versus 22.8% in controls (p=0.001). Positive HIV serology was also found (p=0.001) and was most often passive tobacco related (p=0.015). Respiratory pathology was progressive in 72.9% in cases vs 8.9% (p=0.001) with dyspnoea as the main sign (58%). Tuberculosis (29.70%) was the most common respiratory disease. Maternal complications accounted for 48.1% of PPH vs 25.6% (p=0.001) with maternal mortality of 11.6% (p = 0.001). As for foetal prognosis, 25.6% of foetal complications were noted in patients admitted to PPH versus 48.1% (p = 0.001).Conclusions: Respiratory disease during the gravido puerperal period is severe with significant maternal repercussion.

Abstract A32: NISCH promoter hypermethylation, smoking and lung cancer: A case control study

2016 ◽  
Author(s):  
Kritika Krishnamurthy ◽  
TK Mishra ◽  
Alpana Saxena ◽  
MK Daga ◽  
Nita Khurana ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Case Control Study ◽  
Promoter Hypermethylation ◽  
Case Control ◽  
Control Study

Association of Polymorphism rs1333049 with Risk of Gout

2013 ◽  
Vol 380-384 ◽  
pp. 4167-4169
Author(s):  
Qing Yao Wang ◽  
Hui Yong Yang
Keyword(s):  
Risk Factors ◽  
Coronary Heart Disease ◽  
Statistical Analysis ◽  
Case Control Study ◽  
Case Control ◽  
Coronary Risk ◽  
Pcr Method ◽  
9P21 Locus ◽  
Healthy Participants ◽  
Control Study

Background: Several independent studies have previously demonstrated an association of some polymorphisms in 9p21 locus with coronary risk disease. So this study aimed to evalute whether the SNP rs1333049 in 9p21 locus influenced susceptibility of gout which has been described as a risk factors for coronary heart disease. Methods: One case-control study was performed consisting of 20 healthy participants and 20 patients with gout. Statistical analysis was performed by software SPSS 16.0 afer genotyping with AS-PCR method. Results: The the cases and controls were in conformity with the Hardy-Weinberg law. The genotypes and alleles in this site was no diffence between the cases and the controls. Conclusions: The number of samples is critical for statistical analysis. It is necessary to study larger samples to get small infinite errors in results.

scholarly journals Evaluating NISCH and CDH1 Promoter Hypermethylation in Nonsmokers, Cancer Free Smokers and Lung Cancer Patients: A Case Control Study

2018 ◽  
Vol 34 (4) ◽  
pp. 458-464 ◽  
Author(s):  
Kritika Krishnamurthy ◽  
T. K. Mishra ◽  
Alpana Saxena ◽  
M. K. Daga ◽  
Nita Khurana ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Case Control Study ◽  
Promoter Hypermethylation ◽  
Case Control ◽  
Lung Cancer Patients ◽  
Control Study

scholarly journals Association of CCL5 rs2107538, and CCL2 rs3760396 Gene Pol-ymorphisms with the Risk of Cardiovascular Disease

2021 ◽  
Author(s):  
Naser Mohtavinejad ◽  
Alireza Nakhaee ◽  
Honey Harati ◽  
Nazila Gholipour ◽  
Yavar Mahmoodzade
Keyword(s):  
Haplotype Analysis ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Large Sample Size ◽  
Increased Risk ◽  
History Of ◽  
Cc Chemokines ◽  
Pcr Method ◽  
Control Study

Background: Chemokines are proinflammatory cytokines that play key roles in development of cardiovascular diseases (CVD). Chemokine-induced recruitment of peripheral leucocytes to tissues is a crucial step in the CVD progression. CC chemokines ligand 5, 2 (CCL5 and CCL2), have been characterized as emerging inflammatory biomarkers of atherosclerotic CVD. The aim of this study was to find out whether genetic polymorphisms of CCL5 -403 G>A (rs2107538) and CCL2 –927 G>C, (rs3760396) were associated with the risk of CVD. Methods: In this case-control study, 500 Iranian individuals including 250 CVD patients and 250 healthy subjects as the control group participated in 2017. Genotyping of CCL5 -403 G>A and CCL2 –927 G>C polymorphisms were executed using Tetra-ARMS PCR method. Results: At genotypic level both CCL5 -403 G>A and CCL2 –927 G>C polymorphisms were not associated with the risk of CVD (P>0.05), even after adjustment by age, sex, race, and history of hypertension, DM and smoking. However, the CCL2 –927 C allele was associated with an increased risk of CVD (OR=1.42, P=0.050) with a higher prevalence in CVD patient than in controls (17% vs. 12%). Moreover, the haplotype analysis revealed that CCL5/CCL2 haplotype (G/C) was a risk factor for CVD (OR=2.13, P=0.001), and that carriers of this haplotype were at 2.13-fold higher risk of CVD than subjects with G/G haplotype. Conclusion: CCL2 –927 C variant and CCL5/CCL2 haplotype (G/C) were associated with susceptibility to CVD, and were risk factors for CVD in our population but more studies with large sample size are recommended.

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