scholarly journals Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays

2008 ◽  
Vol 57 (8) ◽  
pp. 1024-1027 ◽  
Author(s):  
Dissou Affolabi ◽  
N'Dira Sanoussi ◽  
Mathieu Odoun ◽  
Anandi Martin ◽  
Louis Koukpemedji ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nitrate Reductase ◽  
Rapid Detection ◽  
Low Cost ◽  
Multidrug Resistant ◽  
Colorimetric Methods

scholarly journals A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

2011 ◽  
Vol 106 (3) ◽  
pp. 378-380 ◽  
Author(s):  
Ahmet Yilmaz Coban ◽  
Yeliz Tanriverdi Cayci ◽  
Aydin Deveci ◽  
Alper Akgunes ◽  
Meltem Uzun ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nitrate Reductase ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Blood Agar ◽  
Nitrate Reductase Assay

scholarly journals Crystal violet decolorization assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates: A multicenter study

2018 ◽  
Vol 7 (4) ◽  
pp. 310
Author(s):  
AhmetYilmaz Coban ◽  
Gumral Alakbarova ◽  
Luqman Satti ◽  
Nadia Tayyab ◽  
Gohar Zaman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Crystal Violet ◽  
Multicenter Study ◽  
Rapid Detection ◽  
Multidrug Resistant

Field evaluation of the direct detection of multidrug resistant Mycobacterium tuberculosis by nitrate reductase assay on 7H11 agar

Tuberculosis ◽  
2013 ◽  
Vol 93 (3) ◽  
pp. 308-311 ◽  
Author(s):  
Luqman Satti ◽  
Aamer Ikram ◽  
Juan Carlos Palomino ◽  
Anandi Martin ◽  
Farooq Ahmad Khan
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nitrate Reductase ◽  
Direct Detection ◽  
Field Evaluation ◽  
Multidrug Resistant ◽  
Nitrate Reductase Assay

scholarly journals Association between embB Codon 306 Mutations and Drug Resistance in Mycobacterium tuberculosis

2007 ◽  
Vol 51 (7) ◽  
pp. 2618-2620 ◽  
Author(s):  
Xin Shen ◽  
Guo-miao Shen ◽  
Jie Wu ◽  
Xiao-hong Gui ◽  
Xia Li ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Odds Ratio ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Candidate Marker ◽  
Resistant Tuberculosis

ABSTRACT embB306 mutants were detected in both ethambutol (EMB)-resistant and EMB-susceptible strains of Mycobacterium tuberculosis. Multidrug-resistant (MDR) strains had a higher proportion of embB306 mutants than non-MDR strains (odds ratio, 6.78; P < 0.001). The embB306 locus is a candidate marker for rapid detection of MDR and extremely drug resistant tuberculosis.

scholarly journals Rapid, Low-Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay

1998 ◽  
Vol 36 (2) ◽  
pp. 362-366 ◽  
Author(s):  
Scott G. Franzblau ◽  
Richard S. Witzig ◽  
James C. McLaughlin ◽  
Patricia Torres ◽  
Guillermo Madico ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Low Cost ◽  
Multidrug Resistant ◽  
Appropriate Technology ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Solid Media ◽  
Resistant Strains ◽  
Stable Reagent ◽  
Initial Results

A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 PeruvianMycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H37Rv strain by using bacterial suspensions prepared directly from solid media. Results for all isolates were available within 8 days. Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 system). The overall agreements between the MICs obtained by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.6% after retesting 12 of 17 samples with discrepant results. Interpretation of MABA endpoints improved with technical experience. The MABA is a simple, rapid, low-cost, appropriate technology which does not require expensive instrumentation and which makes use of a nontoxic, temperature-stable reagent.

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