scholarly journals Evaluation of a PCR-Based Universal Heteroduplex Generator Assay as a Tool for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis in Peru

2003 ◽  
Vol 41 (12) ◽  
pp. 5774-5777 ◽  
Author(s):  
H. Mayta ◽  
R. H. Gilman ◽  
F. Arenas ◽  
T. Valencia ◽  
L. Caviedes ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Multidrug Resistant

scholarly journals Crystal violet decolorization assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates: A multicenter study

2018 ◽  
Vol 7 (4) ◽  
pp. 310
Author(s):  
AhmetYilmaz Coban ◽  
Gumral Alakbarova ◽  
Luqman Satti ◽  
Nadia Tayyab ◽  
Gohar Zaman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Crystal Violet ◽  
Multicenter Study ◽  
Rapid Detection ◽  
Multidrug Resistant

scholarly journals Association between embB Codon 306 Mutations and Drug Resistance in Mycobacterium tuberculosis

2007 ◽  
Vol 51 (7) ◽  
pp. 2618-2620 ◽  
Author(s):  
Xin Shen ◽  
Guo-miao Shen ◽  
Jie Wu ◽  
Xiao-hong Gui ◽  
Xia Li ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Odds Ratio ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Candidate Marker ◽  
Resistant Tuberculosis

ABSTRACT embB306 mutants were detected in both ethambutol (EMB)-resistant and EMB-susceptible strains of Mycobacterium tuberculosis. Multidrug-resistant (MDR) strains had a higher proportion of embB306 mutants than non-MDR strains (odds ratio, 6.78; P < 0.001). The embB306 locus is a candidate marker for rapid detection of MDR and extremely drug resistant tuberculosis.

scholarly journals Evaluation of a line probe assay for the rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis

2011 ◽  
Vol 60 (2) ◽  
pp. 184-188 ◽  
Author(s):  
Hiroki Ando ◽  
Satoshi Mitarai ◽  
Yuji Kondo ◽  
Toshinori Suetake ◽  
Seiya Kato ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Global Health ◽  
Dna Sequencing ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Surveillance Study ◽  
Fluoroquinolone Resistance ◽  
National Surveillance ◽  
Line Probe Assay ◽  
Probe Assay

The aim of this study was to establish the importance of detecting fluoroquinolone (FQ) resistance in multidrug resistant (MDR) Mycobacterium tuberculosis, and to show the usefulness of a hybridization-based line probe assay (LiPA) for detecting gyrA mutations. Thirty-three MDR M. tuberculosis isolates were collected from a total of sixty MDR isolates identified in Japan over 6 months during a national surveillance study in 2002. Seventeen MDR isolates were collected by the National Center for Global Health and Medicine in Japan over 6 years from 2003 to 2008. These 50 isolates were examined for FQ susceptibility, and analysed by LiPA and gyrA sequencing. Among them, 22 (44 %) showed FQ resistance. All FQ-resistant isolates had at least one mutation in gyrA. The results of the LiPA were fully consistent with the DNA sequencing results. Given that on the basis of our results almost half of the MDR M. tuberculosis isolates in Japan might have resistance to FQ, it is important to monitor FQ resistance in patients with MDR tuberculosis (TB), as well as with drug-susceptible TB, prior to commencing treatment. For the detection of FQ resistance, LiPA is useful and can rapidly and efficiently assess FQ resistance.

scholarly journals Mycobacterium tuberculosis Mutations Associated with Reduced Susceptibility to Linezolid

2016 ◽  
Vol 60 (4) ◽  
pp. 2542-2544 ◽  
Author(s):  
Shuo Zhang ◽  
Jiazhen Chen ◽  
Peng Cui ◽  
Wanliang Shi ◽  
Xiaohong Shi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Mechanisms Of Resistance ◽  
Content Type ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Mdr Tb

ABSTRACTLinezolid (LZD) has become increasingly important for the treatment of multidrug-resistant tuberculosis (MDR-TB), but its mechanisms of resistance are not well characterized. We isolated 32 mutants ofMycobacterium tuberculosiswith reduced susceptibility to LZD, which was accounted for byrrlandrplCmutations in almost equal proportions, causing lower and higher MICs, respectively. Our findings provide useful information for the rapid detection of LZD resistance for improved treatment of MDR-TB.

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