scholarly journals Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system

2002 ◽  
Vol 35 (10) ◽  
pp. 1127-1131 ◽  
Author(s):  
M.A.S. Telles ◽  
A. Bori ◽  
A.B.R. Amorim ◽  
A.F. Cruz ◽  
M.I.T. Pini ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Indicator Tube ◽  
Growth Indicator

scholarly journals ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD

2018 ◽  
Vol 11 (3) ◽  
pp. 132
Author(s):  
Retno Wahyuningrum ◽  
Ritmaleni Ritmaleni ◽  
Tatang Irianti ◽  
Subagus Wahyuono ◽  
Takushi Kaneko
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Drug Candidates ◽  
Mycobacterium Tuberculosis H37rv ◽  
Indicator Tube ◽  
Resistant Tuberculosis ◽  
Lowenstein Jensen ◽  
Proportion Method ◽  
Growth Indicator

 Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowenstein–Jensen (LJ) medium.Result: The result of this study showed that ethanolic extract and fractions of T. Crispa did not exhibit anti-TB activity in the range of 100–1000 μg/ml with MGIT method, while with agar proportion method, there were M. tuberculosis colonies growth on the LJ containing 1000 μg/ml extract slants.Conclusion: The tested extract and fractions of T. crispa have no anti-TB activity against M. tuberculosis until 1000 μg/ml.

scholarly journals Crystal violet decolorization assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates: A multicenter study

2018 ◽  
Vol 7 (4) ◽  
pp. 310
Author(s):  
AhmetYilmaz Coban ◽  
Gumral Alakbarova ◽  
Luqman Satti ◽  
Nadia Tayyab ◽  
Gohar Zaman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Crystal Violet ◽  
Multicenter Study ◽  
Rapid Detection ◽  
Multidrug Resistant

scholarly journals Isoniazid Resistance in Mycobacterium tuberculosis Is a Heterogeneous Phenotype Composed of Overlapping MIC Distributions with Different Underlying Resistance Mechanisms

2019 ◽  
Vol 63 (7) ◽  
Author(s):  
Arash Ghodousi ◽  
Elisa Tagliani ◽  
Eranga Karunaratne ◽  
Stefan Niemann ◽  
Jennifer Perera ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Resistance Mechanisms ◽  
Whole Genome ◽  
Tube System ◽  
Isoniazid Resistance ◽  
Content Type ◽  
Indicator Tube ◽  
Promoter Mutations ◽  
Level Resistance ◽  
Growth Indicator

ABSTRACT MIC testing using the Bactec mycobacteria growth indicator tube system 960 of 70 phylogenetically diverse, isoniazid-resistant clinical strains of Mycobacterium tuberculosis revealed a complex pattern of overlapping MIC distributions. Whole-genome sequencing explained most of the levels of resistance observed. The MIC distribution of strains with only inhA promoter mutations was split by the current concentration endorsed by the Clinical and Laboratory Standards Institute to detect low-level resistance to isoniazid and is, consequently, likely not optimally set.

scholarly journals Association between embB Codon 306 Mutations and Drug Resistance in Mycobacterium tuberculosis

2007 ◽  
Vol 51 (7) ◽  
pp. 2618-2620 ◽  
Author(s):  
Xin Shen ◽  
Guo-miao Shen ◽  
Jie Wu ◽  
Xiao-hong Gui ◽  
Xia Li ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Odds Ratio ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Candidate Marker ◽  
Resistant Tuberculosis

ABSTRACT embB306 mutants were detected in both ethambutol (EMB)-resistant and EMB-susceptible strains of Mycobacterium tuberculosis. Multidrug-resistant (MDR) strains had a higher proportion of embB306 mutants than non-MDR strains (odds ratio, 6.78; P < 0.001). The embB306 locus is a candidate marker for rapid detection of MDR and extremely drug resistant tuberculosis.

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