scholarly journals Kodamaea (Pichia) ohmeri fungaemia in a premature neonate

2006 ◽  
Vol 55 (2) ◽  
pp. 237-239 ◽  
Author(s):  
S. J. Taj-Aldeen ◽  
S. H. Doiphode ◽  
X. Y. Han
Keyword(s):  
Amphotericin B ◽  
Liposomal Amphotericin ◽  
Gene Sequencing ◽  
Premature Neonate ◽  
Rrna Gene ◽  
Pathogenic Yeast ◽  
First Case ◽  
Biochemical Identification ◽  
Rrna Gene Sequencing ◽  
Human Infections

Kodamaea ohmeri is a yeast that rarely causes human infections. The first case of K. ohmeri fungaemia in a premature neonate is reported; it was successfully treated with liposomal amphotericin B. Biochemical identification of the yeast was performed by Vitek II and API and was confirmed by rRNA gene sequencing. K. ohmeri as a human pathogenic yeast is uncommon to hospitalized neonates and immunocompromised individuals.

scholarly journals Rhodococcus erythropolis septicaemia in a patient with acute lymphocytic leukaemia

2011 ◽  
Vol 60 (2) ◽  
pp. 252-255 ◽  
Author(s):  
Soon Deok Park ◽  
Young Uh ◽  
In Ho Jang ◽  
Kap Jun Yoon ◽  
Hwang Min Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Lymphocytic Leukaemia ◽  
Rhodococcus Erythropolis ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Acute Lymphocytic Leukaemia ◽  
First Case ◽  
Rrna Gene Sequencing ◽  
Human Infections

Rhodococcus erythropolis rarely causes infection in humans. We report the second case of R. erythropolis septicaemia in a 7-year-old child. However, to our knowledge it is the first case in a patient with acute lymphocytic leukaemia who had been undergoing chemotherapy. The identification was performed using 16S rRNA gene sequencing. Even though R. erythropolis is rarely associated with human infections, it should be considered as a potential causative agent of bacteraemia, rather than overlooked as a contaminant.

scholarly journals First case of an invasive Bacteroides dorei infection detected in a patient with a mycotic aortic aneurysm—raising a rebellion of major indigenous bacteria in humans: a case report and review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Takayuki Matsuoka ◽  
Takuya Shimizu ◽  
Tadanori Minagawa ◽  
Wakiko Hiranuma ◽  
Miki Takeda ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Resected Specimen ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
First Case ◽  
Rrna Gene Sequencing

Abstract Background Bacteroides dorei is an anaerobic gram-negative bacterium first described in 2006. Because of the high similarity in mass spectra between B. dorei and Bacteroides vulgatus, discriminating between these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria, including B. dorei and B. vulgatus, at the genus and species levels. Consequently, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. However, there are no reports on invasive infectious diseases caused by B. dorei. This report describes the first case of direct invasion and colonisation of human tissue by B. dorei, thus providing a warning regarding the previously proposed application of B. dorei as a live biotherapeutic for atherosclerotic diseases. Case presentation A 78-year-old Japanese man complained of intermittent chest/back pain and was diagnosed with a mycotic thoracic aortic aneurysm by enhanced computed tomography on admission. Despite strict blood pressure control and empirical antibiotic therapy, the patient’s condition worsened. To prevent aneurysmal rupture and eliminate infectious foci, the patient underwent surgical treatment. The resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/B. vulgatus and B. dorei, respectively. The patient was successfully treated with appropriate antibacterial therapy and after improvement, was transferred to another hospital for rehabilitation on postoperative day 34. There was no recurrence of infection or aneurysm after the patient transfer. Conclusions This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

scholarly journals Septic arthritis due to Roseomonas gilardii in an immunocompetent adolescent

2009 ◽  
Vol 58 (11) ◽  
pp. 1514-1516 ◽  
Author(s):  
Sergio Fanella ◽  
Daryl Schantz ◽  
James Karlowsky ◽  
Ethan Rubinstein
Keyword(s):  
Septic Arthritis ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Sports Injury ◽  
Bordetella Bronchiseptica ◽  
Rrna Gene ◽  
First Case ◽  
Vitek 2 ◽  
Rrna Gene Sequencing ◽  
Slow Growing

The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are associated with immunocompromised adults, often causing bacteraemia. Due to their rarity, members of this genus can be overlooked or misidentified using automated laboratory identification systems. We report on an immunocompetent adolescent patient who developed septic arthritis due to Roseomonas gilardii following surgery for a sports injury. The isolate was initially misidentified as Bordetella bronchiseptica using the Vitek 2 system, but confirmed as R. gilardii based on 16S rRNA gene sequencing. To the best of our knowledge, this is the first case of a healthy paediatric patient with septic arthritis due to R. gilardii.

scholarly journals Sepsis Due to a Novel Urease-Positive Helicobacter Species in a Young Man

2015 ◽  
Vol 53 (7) ◽  
pp. 2378-2380 ◽  
Author(s):  
Nobuaki Mori ◽  
Narito Kagawa ◽  
Akiko Higuchi ◽  
Yasuko Aoki ◽  
Kiyofumi Ohkusu
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Short Term ◽  
Content Type ◽  
First Case ◽  
Rrna Gene Sequencing

We report the first case of sepsis with enterocolitis that was caused by a novel urease-positiveHelicobacterspecies in a young man. The isolate was characterized via 16S rRNA gene sequencing and their biochemical properties, and the patient was successfully treated with short-term antimicrobial therapy; no recurrence was observed.

scholarly journals hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria

1999 ◽  
Vol 37 (3) ◽  
pp. 852-857 ◽  
Author(s):  
H. Ringuet ◽  
C. Akoua-Koffi ◽  
S. Honore ◽  
A. Varnerot ◽  
V. Vincent ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Mycobacterium Abscessus ◽  
Rrna Gene ◽  
Mycobacterium Fortuitum ◽  
Mycobacterium Chelonae ◽  
Rapidly Growing Mycobacteria ◽  
Rrna Gene Sequencing ◽  
Human Infections ◽  
Type Strains

Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175–178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a unique nucleotide sequence, distinguishing it clearly from the other species. A panel of strains from the four main RGM species responsible for human infections, Mycobacterium abscessus, Mycobacterium chelonae,Mycobacterium fortuitum, and Mycobacterium peregrinum, was also studied. There were few sequence differences within each of these species (<2% of bases were different from the type strain sequence), and they had no effect on species assignment.hsp65 sequencing unambiguously differentiated M. chelonae and M. abscessus, two species difficult to identify by classical methods and 16S rRNA gene sequencing. The devised procedure is a rapid and reliable tool for the identification of RGM species.

scholarly journals The First Case of Invasive Bacteroides Dorei Infection Detected in a Mycotic Aortic Aneurysm Patient-Raising A Rebellion of Major Indigenous Bacteria in Humans: A Case Report and Review

2020 ◽  
Author(s):  
Takayuki Matsuoka ◽  
Takuya Shimizu ◽  
Tadanori Minagawa ◽  
Wakiko Hiranuma ◽  
Miki Takeda ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Aortic Aneurysm ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Resected Specimen ◽  
Rrna Gene ◽  
First Case ◽  
Rrna Gene Sequencing

Abstract Background: Bacteroides dorei is an anaerobic, gram-negative bacterium first described in 2006. Due to the high similarity in mass spectrum patterns between B. dorei and Bacteroides vulgatus, discriminating these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria to the genus and species levels, including B. dorei and B. vulgatus. Accordingly, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. Nevertheless, there are no reports on invasive infectious disease caused by B. dorei. This report describes the first case of B. dorei presenting direct invasion and colonisation into human tissue, providing a warning for the previously proposed application of B. dorei as live biotherapeutics for atherosclerotic diseases.Case presentation: A 78-year-old man admitted with suspicion for mycotic thoracic aortic aneurysm was diagnosed by enhanced computed tomography scan, exhibiting the appearance of infection and dissection at the distal aortic arch. Despite strict blood pressure control and empirical antibiotic therapy, the patient's condition worsened. For the prevention of aneurysmal rupture and elimination of infectious foci, the patient underwent surgical treatment, and the resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/vulgatus and B. dorei, respectively. The patient was successfully treated by appropriate antibacterial therapy, improved and was transferred to another hospital for rehabilitation at postoperative day 34. There was no recurrence of symptoms after the patient transfer.Conclusions: This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

Screening and Molecular Characterization of Cellulase Producing Actinobacteria from Litchi Orchard

2019 ◽  
Vol 13 (1) ◽  
pp. 90-101
Author(s):  
Sanju Kumari ◽  
Utkarshini Sharma ◽  
Rohit Krishna ◽  
Kanak Sinha ◽  
Santosh Kumar
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Evolutionary Relationship ◽  
Gene Sequencing ◽  
Cellulase Activity ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Background: Cellulolysis is of considerable economic importance in laundry detergents, textile and pulp and paper industries and in fermentation of biomass into biofuels. Objective: The aim was to screen cellulase producing actinobacteria from the fruit orchard because of its requirement in several chemical reactions. Methods: Strains of actinobacteria were isolated on Sabouraud’s agar medium. Similarities in cultural and biochemical characterization by growing the strains on ISP medium and dissimilarities among them perpetuated to recognise nine groups of actinobacteria. Cellulase activity was measured by the diameter of clear zone around colonies on CMC agar and the amount of reducing sugar liberated from carboxymethyl cellulose in the supernatant of the CMC broth. Further, 16S rRNA gene sequencing and molecular characterization were placed before NCBI for obtaining recognition with accession numbers. Results: Prominent clear zones on spraying Congo Red were found around the cultures of strains of three groups SK703, SK706, SK708 on CMC agar plates. The enzyme assay for carboxymethylcellulase displayed extra cellulase activity in broth: 0.14, 0.82 and 0.66 &#181;mol mL-1 min-1, respectively at optimum conditions of 35°C, pH 7.3 and 96 h of incubation. However, the specific cellulase activities per 1 mg of protein did not differ that way. It was 1.55, 1.71 and 1.83 μmol mL-1 min-1. The growing mycelia possessed short compact chains of 10-20 conidia on aerial branches. These morphological and biochemical characteristics, followed by their verification by Bergey’s Manual, categorically allowed the strains to be placed under actinobacteria. Further, 16S rRNA gene sequencing, molecular characterization and their evolutionary relationship through phylogenetics also confirmed the putative cellulase producing isolates of SK706 and SK708 subgroups to be the strains of Streptomyces. These strains on getting NCBI recognition were christened as Streptomyces glaucescens strain SK91L (KF527284) and Streptomyces rochei strain SK78L (KF515951), respectively. Conclusion: Conclusive evidence on the basis of different parameters established the presence of cellulase producing actinobacteria in the litchi orchard which can convert cellulose into fermentable sugar.

Sign in / Sign up

Export Citation Format

Share Document