First case of an invasive Bacteroides dorei infection detected in a patient with a mycotic aortic aneurysm—raising a rebellion of major indigenous bacteria in humans: a case report and review

Abstract Background Bacteroides dorei is an anaerobic gram-negative bacterium first described in 2006. Because of the high similarity in mass spectra between B. dorei and Bacteroides vulgatus, discriminating between these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria, including B. dorei and B. vulgatus, at the genus and species levels. Consequently, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. However, there are no reports on invasive infectious diseases caused by B. dorei. This report describes the first case of direct invasion and colonisation of human tissue by B. dorei, thus providing a warning regarding the previously proposed application of B. dorei as a live biotherapeutic for atherosclerotic diseases. Case presentation A 78-year-old Japanese man complained of intermittent chest/back pain and was diagnosed with a mycotic thoracic aortic aneurysm by enhanced computed tomography on admission. Despite strict blood pressure control and empirical antibiotic therapy, the patient’s condition worsened. To prevent aneurysmal rupture and eliminate infectious foci, the patient underwent surgical treatment. The resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/B. vulgatus and B. dorei, respectively. The patient was successfully treated with appropriate antibacterial therapy and after improvement, was transferred to another hospital for rehabilitation on postoperative day 34. There was no recurrence of infection or aneurysm after the patient transfer. Conclusions This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

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The First Case of Invasive Bacteroides Dorei Infection Detected in a Mycotic Aortic Aneurysm Patient-Raising A Rebellion of Major Indigenous Bacteria in Humans: A Case Report and Review

10.21203/rs.3.rs-74102/v1 ◽

2020 ◽

Author(s):

Takayuki Matsuoka ◽

Takuya Shimizu ◽

Tadanori Minagawa ◽

Wakiko Hiranuma ◽

Miki Takeda ◽

...

Keyword(s):

Infectious Disease ◽

Aortic Aneurysm ◽

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Resected Specimen ◽

Rrna Gene ◽

First Case ◽

Rrna Gene Sequencing

Abstract Background: Bacteroides dorei is an anaerobic, gram-negative bacterium first described in 2006. Due to the high similarity in mass spectrum patterns between B. dorei and Bacteroides vulgatus, discriminating these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria to the genus and species levels, including B. dorei and B. vulgatus. Accordingly, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. Nevertheless, there are no reports on invasive infectious disease caused by B. dorei. This report describes the first case of B. dorei presenting direct invasion and colonisation into human tissue, providing a warning for the previously proposed application of B. dorei as live biotherapeutics for atherosclerotic diseases.Case presentation: A 78-year-old man admitted with suspicion for mycotic thoracic aortic aneurysm was diagnosed by enhanced computed tomography scan, exhibiting the appearance of infection and dissection at the distal aortic arch. Despite strict blood pressure control and empirical antibiotic therapy, the patient's condition worsened. For the prevention of aneurysmal rupture and elimination of infectious foci, the patient underwent surgical treatment, and the resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/vulgatus and B. dorei, respectively. The patient was successfully treated by appropriate antibacterial therapy, improved and was transferred to another hospital for rehabilitation at postoperative day 34. There was no recurrence of symptoms after the patient transfer.Conclusions: This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

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Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis

The Open Microbiology Journal ◽

10.2174/1874285801004010123 ◽

2010 ◽

Vol 4 (1) ◽

pp. 123-131 ◽

Cited By ~ 6

Author(s):

Jens JØrgen Christensen ◽

Brita Bruun ◽

Ute Wolff Sönksen ◽

Lisbeth Nielsen ◽

Annemarie Hesselbjerg ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Sequencing Analysis ◽

Vitek 2 ◽

Rrna Gene Sequencing

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Bacterial composition of nasal discharge in children based on highly accurate 16S rRNA gene sequencing analysis

Scientific Reports ◽

10.1038/s41598-020-77271-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Kaoru Haro ◽

Midori Ogawa ◽

Mitsumasa Saito ◽

Koichi Kusuhara ◽

Kazumasa Fukuda

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Nasal Discharge ◽

Rrna Gene ◽

Respiratory Pathogens ◽

Sequencing Analysis ◽

Bacterial Cells ◽

Rrna Gene Sequencing

AbstractNasopharyngeal colonization by bacteria is a prerequisite for progression to respiratory disease and an important source of horizontal spread within communities. We aimed to perform quantitative analysis of the bacterial cells and reveal the microbiota of the nasal discharge in children at the species level based on highly accurate 16S rRNA gene sequencing. This study enrolled 40 pediatric patients with rhinorrhea. The bacterial cells in the nasal discharge were counted by epifluorescence microscopic analysis. The microbiota was analyzed by using the 16S rRNA gene clone library sequencing method. We demonstrated that a high abundance (median 2.2 × 107 cells/mL) of bacteria was contained in the nasal discharge of children. Of the 40 samples, 37 (92.5%) were dominated by OTUs corresponding to Haemophilus aegyptius/influenzae, Moraxella catarrhalis/nonliquefaciens, or Streptococcus pneumoniae. These samples showed higher cell abundance and lower alpha diversity than the remaining three samples in which the other bacteria coexisted. In addition, 12 sequences with low homology to type strains were considered as previously unknown bacterial lineages. In conclusion, the nasal discharge of most young children contains a large amount of respiratory pathogens and several unknown bacteria, which could not only cause endogenous infection but also be a source of transmission to others.

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Globicatella sanguinis Bacteremia in a Non-Immunocompromised Patient Identified by 16S rRNA Gene Sequencing: First Case in Korea

Clinical Laboratory ◽

10.7754/clin.lab.2016.151215 ◽

2016 ◽

Vol 62 (09/2016) ◽

Cited By ~ 1

Author(s):

Hyung-Seok Yang ◽

Young Kim ◽

Mi Lee ◽

Hee Lee

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Immunocompromised Patient ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

First Case ◽

Rrna Gene Sequencing

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Use of 16S rRNA gene sequencing analysis to characterize culturable intestinal bacteria in Atlantic salmon (Salmo salar) fed diets with cellulose or non-starch polysaccharides from soy

Aquaculture Research ◽

10.1111/j.1365-2109.2008.01972.x ◽

2008 ◽

Vol 39 (10) ◽

pp. 1087-1100 ◽

Cited By ~ 57

Author(s):

Einar Ringø ◽

Sigmund Sperstad ◽

Olav Fjeld Kraugerud ◽

Åshild Krogdahl

Keyword(s):

16S Rrna ◽

Atlantic Salmon ◽

16S Rrna Gene ◽

Salmo Salar ◽

Gene Sequencing ◽

Intestinal Bacteria ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Sequencing Analysis ◽

Rrna Gene Sequencing

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Severe Pneumonia with Leptotrichia sp. Detected Predominantly in Bronchoalveolar Lavage Fluid by Use of 16S rRNA Gene Sequencing Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.01429-08 ◽

2008 ◽

Vol 47 (2) ◽

pp. 496-498 ◽

Cited By ~ 20

Author(s):

T. Kawanami ◽

K. Fukuda ◽

K. Yatera ◽

T. Kido ◽

C. Yoshii ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bronchoalveolar Lavage Fluid ◽

Gene Sequencing ◽

Severe Pneumonia ◽

16S Rrna Gene Sequencing ◽

Lavage Fluid ◽

Rrna Gene ◽

Sequencing Analysis ◽

Rrna Gene Sequencing

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Sepsis Due to a Novel Urease-Positive Helicobacter Species in a Young Man

Journal of Clinical Microbiology ◽

10.1128/jcm.00439-15 ◽

2015 ◽

Vol 53 (7) ◽

pp. 2378-2380 ◽

Cited By ~ 1

Author(s):

Nobuaki Mori ◽

Narito Kagawa ◽

Akiko Higuchi ◽

Yasuko Aoki ◽

Kiyofumi Ohkusu

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Biochemical Properties ◽

Rrna Gene ◽

Short Term ◽

Content Type ◽

First Case ◽

Rrna Gene Sequencing

We report the first case of sepsis with enterocolitis that was caused by a novel urease-positiveHelicobacterspecies in a young man. The isolate was characterized via 16S rRNA gene sequencing and their biochemical properties, and the patient was successfully treated with short-term antimicrobial therapy; no recurrence was observed.

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