scholarly journals Influence of the chloride channel of Fusarium oxysporum on extracellular laccase activity and virulence on tomato plants

Microbiology ◽  
2008 ◽  
Vol 154 (5) ◽  
pp. 1474-1481 ◽  
Author(s):  
Dolores Cordoba Cañero ◽  
M. Isabel G. Roncero
Keyword(s):  
Fusarium Oxysporum ◽  
Chloride Channel ◽  
Laccase Activity ◽  
Tomato Plants ◽  
Extracellular Laccase

scholarly journals Functional Analyses of Laccase Genes from Fusarium oxysporum

2008 ◽  
Vol 98 (5) ◽  
pp. 509-518 ◽  
Author(s):  
D. Cordoba Cañero ◽  
M. I. G. Roncero
Keyword(s):  
Fusarium Oxysporum ◽  
Phenol Oxidase ◽  
Infection Process ◽  
Copper Binding ◽  
Tomato Plants ◽  
Extracellular Laccase ◽  
Polymerase Chain ◽  
Laccase Genes ◽  
Functional Analyses ◽  
Targeted Inactivation

Six laccase genes, lcc1, lcc2, lcc3, lcc4, lcc5, and lcc9, of the vascular wilt fungus Fusarium oxysporum were isolated and characterized. All genes have the characteristic conserved domains for copper binding of phenol oxidase enzymes. Targeted inactivation of lcc1, lcc3, and lcc5 resulted in a significant decrease of extracellular laccase activity. Reverse transcription-polymerase chain reaction showed that lcc1, lcc2, and lcc9 were constitutively expressed in culture, whereas lcc3 and lcc5 appeared down and up-regulated, respectively, by PacC. Oxidative stress conditions and phenolic compounds altered the growth rate of the Δlcc3 mutant compared with the wild-type. lcc1, lcc3, and lcc9 were expressed in roots and stems during the infection process. However, inactivation of lcc1, lcc3, and lcc5 had no detectable effects on virulence on tomato plants.

Race identification of Fusarium oxysporum f. sp. lycopersici isolates obtained from tomato plants in Nova Friburgo, Brazil

2021 ◽  
Author(s):  
Cristiana Maia de Oliveira ◽  
Margarida Gorete Ferreira do Carmo ◽  
Leandro Martins Ferreira ◽  
Monica Höfte ◽  
Nelson Moura Brasil do Amaral Sobrinho
Keyword(s):  
Fusarium Oxysporum ◽  
Tomato Plants ◽  
Race Identification

scholarly journals Signaling in the Tomato Immunity against Fusarium oxysporum

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1818
Author(s):  
Francisco Hernández-Aparicio ◽  
Purificación Lisón ◽  
Ismael Rodrigo ◽  
José María Bellés ◽  
M. Pilar López-Gresa
Keyword(s):  
Fusarium Oxysporum ◽  
Environmental Sustainability ◽  
Control Strategies ◽  
Marker Gene ◽  
Crop Protection ◽  
Incompatible Interaction ◽  
Tomato Plants ◽  
Fatty Acid Derivatives ◽  
Resistance Inducers ◽  
Plant Pathogen Interactions

New strategies of control need to be developed with the aim of economic and environmental sustainability in plant and crop protection. Metabolomics is an excellent platform for both understanding the complex plant–pathogen interactions and unraveling new chemical control strategies. GC-MS-based metabolomics, along with a phytohormone analysis of a compatible and incompatible interaction between tomato plants and Fusarium oxysporum f. sp. lycopersici, revealed the specific volatile chemical composition and the plant signals associated with them. The susceptible tomato plants were characterized by the over-emission of methyl- and ethyl-salicylate as well as some fatty acid derivatives, along with an activation of salicylic acid and abscisic acid signaling. In contrast, terpenoids, benzenoids, and 2-ethylhexanoic acid were differentially emitted by plants undergoing an incompatible interaction, together with the activation of the jasmonic acid (JA) pathway. In accordance with this response, a higher expression of several genes participating in the biosynthesis of these volatiles, such as MTS1, TomloxC,TomloxD, and AOS, as well as JAZ7, a JA marker gene, was found to be induced by the fungus in these resistant plants. The characterized metabolome of the immune tomato plants could lead to the development of new resistance inducers against Fusarium wilt treatment.

scholarly journals A Molecular Vision of the Interaction of Tomato Plants and Fusarium oxysporum f. sp. lycopersic

2018 ◽  
Author(s):  
Ema Laura García-Enciso ◽  
Adalberto Benavides-Mendoza ◽  
María Liliana Flores-López ◽  
Armando Robledo-Olivo ◽  
Antonio Juárez-Maldonado ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Tomato Plants

scholarly journals Fusarium oxysporum colonizes the stem of resistant tomato plants, the extent varying with the R-gene present

2018 ◽  
Vol 154 (1) ◽  
pp. 55-65 ◽  
Author(s):  
H. C. van der Does ◽  
M. E. Constantin ◽  
P. M. Houterman ◽  
F. L. W. Takken ◽  
B. J. C. Cornelissen ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
R Gene ◽  
Tomato Plants

scholarly journals Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato

2008 ◽  
Vol 43 (8) ◽  
pp. 1017-1023 ◽  
Author(s):  
Daniel Oliveira Jordão do Amaral ◽  
Marleide Magalhães de Andrade Lima ◽  
Luciane Vilela Resende ◽  
Márcia Vanusa da Silva
Keyword(s):  
Salicylic Acid ◽  
Fusarium Oxysporum ◽  
Foliar Application ◽  
Cost Effective ◽  
Subtractive Hybridization ◽  
Differentially Expressed ◽  
Transcript Profile ◽  
Tomato Plants ◽  
New Genes ◽  
Differential Gene

The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH technique is effective for identification of resistance genes activated by salicylic acid and F. oxysporum f. sp. lycopersici infection. Not only the application of this technique enables a cost effective isolation of differentially expressed sequences, but also it allows the identification of novel sequences in tomato from a relative small number of sequences.

scholarly journals Treatment with the Mycoparasite Pythium oligandrum Triggers Induction of Defense-Related Reactions in Tomato Roots When Challenged with Fusarium oxysporum f. sp. radicis-lycopersici

1997 ◽  
Vol 87 (1) ◽  
pp. 108-122 ◽  
Author(s):  
Nicole Benhamou ◽  
Patrice Rey ◽  
Mohamed Chérif ◽  
John Hockenhull ◽  
Yves Tirilly
Keyword(s):  
Plant Defense ◽  
Fusarium Oxysporum ◽  
Host Cell ◽  
Cell Walls ◽  
Pythium Oligandrum ◽  
Tomato Plants ◽  
Host Cytoplasm ◽  
Defense Reactions ◽  
Tomato Roots ◽  
Root Tissues

The influence exerted by the mycoparasite Pythium oligandrum in triggering plant defense reactions was investigated using an experimental system in which tomato plants were infected with the crown and root rot pathogen Fusarium oxysporum f. sp. radicis-lycopersici. To assess the antagonistic potential of P. oligandrum against F. oxysporum f. sp. radicis-lycopersici, the interaction between the two fungi was studied by scanning and transmission electron microscopy (SEM and TEM, respectively). SEM investigations of the interaction region between the fungi demonstrated that collapse and loss of turgor of F. oxysporum f. sp. radicis-lycopersici hyphae began soon after close contact was established with P. oligandrum. Ultrastructural observations confirmed that intimate contact between hyphae of P. oligandrum and cells of the pathogen resulted in a series of disturbances, including generalized disorganization of the host cytoplasm, retraction of the plasmalemma, and, finally, complete loss of the protoplasm. Cytochemical labeling of chitin with wheat germ agglutinin (WGA)/ovomucoid-gold complex showed that, except in the area of hyphal penetration, the chitin component of the host cell walls was structurally preserved at a time when the host cytoplasm had undergone complete disorganization. Interestingly, the same antagonistic process was observed in planta. The specific labeling patterns obtained with the exoglucanase-gold and WGA-ovomucoid-gold complexes confirmed that P. oligandrum successfully penetrated invading cells of the pathogen without causing substantial cell wall alterations, shown by the intense labeling of chitin. Cytological investigations of samples from P. oligandrum-inoculated tomato roots revealed that the fungus was able to colonize root tissues without inducing extensive cell damage. However, there was a novel finding concerning the structural alteration of the invading hyphae, evidenced by the frequent occurrence of empty fungal shells in root tissues. Pythium ingress in root tissues was associated with host metabolic changes, culminating in the elaboration of structural barriers at sites of potential fungal penetration. Striking differences in the extent of F. oxysporum f. sp. radicis-lycopersici colonization were observed between P. oligandrum-inoculated and control tomato plants. In control roots, the pathogen multiplied abundantly through much of the tissues, whereas in P. oligandrum-colonized roots pathogen growth was restricted to the outermost root tissues. This restricted pattern of pathogen colonization was accompanied by deposition of newly formed barriers beyond the infection sites. These host reactions appeared to be amplified compared to those seen in nonchallenged P. oligandrum-infected plants. Most hyphae of the pathogen that penetrated the epidermis exhibited considerable changes. Wall appositions contained large amounts of callose, in addition to be infiltrated with phenolic compounds. The labeling pattern obtained with gold-complexed laccase showed that phenolics were widely distributed in Fusarium-challenged P. oligandrum-inoculated tomato roots. Such compounds accumulated in the host cell walls and intercellular spaces. The wall-bound chitin component in Fusarium hyphae colonizing P. oligandrum-inoculated roots was preserved at a time when hyphae had undergone substantial degradation. These observations provide the first convincing evidence that P. oligandrum has the potential to induce plant defense reactions in addition to acting as a mycoparasite.

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