scholarly journals Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae

2015 ◽  
Vol 96 (4) ◽  
pp. 915-920 ◽  
Author(s):  
Mutien-Marie Garigliany ◽  
Jessica Börstler ◽  
Hanna Jöst ◽  
Marlis Badusche ◽  
Daniel Desmecht ◽  
...  
Keyword(s):  
New Genus ◽  
Aedes Vexans ◽  
The Family

scholarly journals Characterization of a new picornavirus isolated from the freshwater fish Lepomis macrochirus

2014 ◽  
Vol 95 (3) ◽  
pp. 601-613 ◽  
Author(s):  
Marisa Barbknecht ◽  
Sol Sepsenwol ◽  
Eric Leis ◽  
Maren Tuttle-Lau ◽  
Mark Gaikowski ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
New Genus ◽  
Lepomis Macrochirus ◽  
Leader Peptide ◽  
Fish Kill ◽  
Sequence Identity ◽  
Phylogenetic Cluster ◽  
The Family ◽  
Sport Fish

The freshwater fish Lepomis macrochirus (bluegill) is common to North American waters, and important both ecologically and as a sport fish. In 2001 an unknown virus was isolated from bluegills following a bluegill fish kill. This virus was identified as a picornavirus [termed bluegill picornavirus (BGPV)] and a diagnostic reverse transcriptase PCR was developed. A survey of bluegills in Wisconsin waters showed the presence of BGPV in 5 of 17 waters sampled, suggesting the virus is widespread in bluegill populations. Experimental infections of bluegills confirmed that BGPV can cause morbidity and mortality in bluegills. Molecular characterization of BGPV revealed several distinct genome characteristics, the most unusual of which is the presence of a short poly(C) tract in the 3′ UTR. Additionally, the genome encodes a polyprotein lacking a leader peptide and a VP0 maturation cleavage site, and is predicted to encode two distinct 2A proteins. Sequence comparison showed that the virus is most closely related to a phylogenetic cluster of picornaviruses that includes the genera Aquamavirus, Avihepatovirus and Parechovirus. However, it is distinct enough, for example sharing only about 38 % sequence identity to the parechoviruses in the 3D region, that it may represent a new genus in the family Picornaviridae.

scholarly journals Characterization of a novel member of the family Caulimoviridae infecting Dioscorea nummularia in the Pacific, which may represent a new genus of dsDNA plant viruses

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0203038 ◽  
Author(s):  
Amit C. Sukal ◽  
Dawit B. Kidanemariam ◽  
James L. Dale ◽  
Robert M. Harding ◽  
Anthony P. James
Keyword(s):  
New Genus ◽  
Plant Viruses ◽  
The Pacific ◽  
The Family

Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae

2008 ◽  
Vol 153 (7) ◽  
pp. 1263-1270 ◽  
Author(s):  
Anna Maria Vaira ◽  
Clarissa J. Maroon-Lango ◽  
John Hammond
Keyword(s):  
Molecular Characterization ◽  
New Genus ◽  
Latent Virus ◽  
The Family

scholarly journals Genomic characterization of three novel Pseudomonas phages within the subfamily of Autographivirinae isolated from organic waste

2020 ◽  
Author(s):  
Jacob B Jørgensen ◽  
Amaru M Djuurhus ◽  
Alexander B. Carstens ◽  
Witold Kot ◽  
Cindy E. Morris ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Organic Waste ◽  
New Genus ◽  
Open Reading Frames ◽  
Genomic Comparison ◽  
Double Stranded Dna ◽  
The Family ◽  
Genomic Characterization ◽  
Reading Frames

AbstractThree phages targeting Pseudomonas syringae GAW0113 have been isolated from organic waste samples: Pseudomonas phage Bertil, Misse and Strit. The phages have double-stranded DNA genomes ranging from 41342 to 41374 bp in size comprising 50 to 51 open reading frames. The three phages genomes are highly similar and genomic comparison analyses shows that they all belong to the Autographivirinae subfamily of the family Podoviridae. The phages are however only distantly related to other members of this family, and have limited gene synteny with type-phages of other genera within Autographivirinae, suggesting that the newly isolated phages could represent a new genus.

Detection and characterization of a novel rhabdovirus in Aedes cantans mosquitoes and evidence for a mosquito-associated new genus in the family Rhabdoviridae

2017 ◽  
Vol 55 ◽  
pp. 260-268 ◽  
Author(s):  
Nariman Shahhosseini ◽  
Renke Lühken ◽  
Hanna Jöst ◽  
Stephanie Jansen ◽  
Jessica Börstler ◽  
...  
Keyword(s):  
New Genus ◽  
The Family ◽  
Family Rhabdoviridae

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

Paralypusa, a new genus of the family Lypusidae (Lepidoptera: Gelechioidea) from China

2012 ◽  
Vol 21 (1) ◽  
pp. 169-172
Author(s):  
A.L. Lvovsky
Keyword(s):  
New Genus ◽  
Monotypic Genus ◽  
Transitional State ◽  
The Family

A new eastern-palaearctic monotypic genus of the gelechioid moths, Paralypusa gen. nov., is established for the Chinese species Paralypusa chinensis (Lvovsky, 2010), comb. nov. This genus is considered closely related to Lypusa Zeller, 1852 on the base of several synapomorphies. However Paralypusa also shares some features with the genera Pseudatemelia Rebel, 1910 and Amphisbatis Zeller, 1870. Its transitional state confirms the belonging of all the aforementioned genera to one family and synonymy of the Lypusidae Herrich-Schäffer, 1857 and Amphisbatidae Spuler, 1910 (Nieukerken et al., 2011).

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