scholarly journals Characterization of a new picornavirus isolated from the freshwater fish Lepomis macrochirus

2014 ◽  
Vol 95 (3) ◽  
pp. 601-613 ◽  
Author(s):  
Marisa Barbknecht ◽  
Sol Sepsenwol ◽  
Eric Leis ◽  
Maren Tuttle-Lau ◽  
Mark Gaikowski ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
New Genus ◽  
Lepomis Macrochirus ◽  
Leader Peptide ◽  
Fish Kill ◽  
Sequence Identity ◽  
Phylogenetic Cluster ◽  
The Family ◽  
Sport Fish

The freshwater fish Lepomis macrochirus (bluegill) is common to North American waters, and important both ecologically and as a sport fish. In 2001 an unknown virus was isolated from bluegills following a bluegill fish kill. This virus was identified as a picornavirus [termed bluegill picornavirus (BGPV)] and a diagnostic reverse transcriptase PCR was developed. A survey of bluegills in Wisconsin waters showed the presence of BGPV in 5 of 17 waters sampled, suggesting the virus is widespread in bluegill populations. Experimental infections of bluegills confirmed that BGPV can cause morbidity and mortality in bluegills. Molecular characterization of BGPV revealed several distinct genome characteristics, the most unusual of which is the presence of a short poly(C) tract in the 3′ UTR. Additionally, the genome encodes a polyprotein lacking a leader peptide and a VP0 maturation cleavage site, and is predicted to encode two distinct 2A proteins. Sequence comparison showed that the virus is most closely related to a phylogenetic cluster of picornaviruses that includes the genera Aquamavirus, Avihepatovirus and Parechovirus. However, it is distinct enough, for example sharing only about 38 % sequence identity to the parechoviruses in the 3D region, that it may represent a new genus in the family Picornaviridae.

scholarly journals IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase from Chryseobacterium indologenes Strain 597 Isolated in Burkina Faso

2009 ◽  
Vol 53 (10) ◽  
pp. 4320-4326 ◽  
Author(s):  
Boukaré Zeba ◽  
Filomena De Luca ◽  
Alain Dubus ◽  
Michael Delmarcelle ◽  
Jacques Simporé ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Posttranslational Modification ◽  
Human Pathogens ◽  
Turnover Rates ◽  
Chryseobacterium Indologenes ◽  
Sequence Identity ◽  
New Variant ◽  
The Family ◽  
Identification And Characterization

ABSTRACT The genus Chryseobacterium and other genera belonging to the family Flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of Flavobacteriaceae, including Chryseobacterium indologenes, are naturally resistant to β-lactam antibiotics (including carbapenems), due to the production of a resident metallo-β-lactamase. Although C. indologenes presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a C. indologenes isolate from Burkina Faso that is resistant to β-lactams and aminoglycosides. The levels of sequence identity of the new variant to other IND-type metallo-β-lactamases range between 72 and 90% (for IND-4 and IND-5, respectively). The purified enzyme exhibited N-terminal heterogeneity and a posttranslational modification consisting of the presence of a pyroglutamate residue at the N terminus. IND-6 shows a broad substrate profile, with overall higher turnover rates than IND-5 and higher activities than IND-2 and IND-5 against ceftazidime and cefepime.

scholarly journals Terasakiispira papahanaumokuakeensis gen. nov., sp. nov., a gammaproteobacterium from Pearl and Hermes Atoll, Northwestern Hawaiian Islands

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3609-3617 ◽  
Author(s):  
Vanessa K. Zepeda ◽  
Hans-Jürgen Busse ◽  
Jan Golke ◽  
Jimmy H. W. Saw ◽  
Maqsudul Alam ◽  
...  
Keyword(s):  
Nucleotide Sequence Identity ◽  
Type Strain ◽  
Type Species ◽  
New Genus ◽  
Hawaiian Islands ◽  
Rrna Gene ◽  
Sequence Identity ◽  
The Family ◽  
Northwestern Hawaiian Islands ◽  
Hydrolysis Of

A Gram-negative, helical bacterium designated PH27AT was cultivated from an anchialine pool on Pearl and Hermes Atoll, Northwestern Hawaiian Islands. The obligately halophilic strain was motile by bipolar tufts of flagella and grew optimally at pH 7, and microaerobically or aerobically. Closest neighbours based on 16S rRNA gene nucleotide sequence identity are Marinospirillum celere v1c_Sn-redT (93.31 %) and M. alkaliphilum Z4T (92.10 %) in the family Oceanospirillaceae, class Gammaproteobacteria. PH27AT is distinguished phenotypically from members of the genus Marinospirillum by its hydrolysis of gelatin, the absence of growth in media containing ≤ 1 % (w/v) NaCl and the ranges of temperature (12–40 °C) and pH (5–8) for growth. The major compound ubiquinone Q-9 distinguishes the quinone system of strain PH27AT from those in members of the genus Marinospirillum and other members of the Oceanospirillaceae, in which the major quinone is Q-8. Major polar lipids in PH27AT were phosphatidylethanolamine and phosphatidylglycerol, with moderate amounts of diphosphatidylglycerol and phosphatidylserine. Spermidine and cadaverine dominated the polyamine pattern; large proportions of cadaverine have not been reported in members of the genus Marinospirillum. Genotypic and chemotaxonomic data show that PH27AT does not belong in the genus Marinospirillum or other genera of the family Oceanospirillaceae or the Halomonadaceae. We propose a new genus, Terasakiispira gen. nov., be created to accommodate Terasakiispira papahanaumokuakeensis gen. nov., sp. nov. as the type species, with PH27AT ( = ATCC BAA-995T = DSM 16455T = DSM 23961T) as the type strain.

scholarly journals Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae

2015 ◽  
Vol 96 (4) ◽  
pp. 915-920 ◽  
Author(s):  
Mutien-Marie Garigliany ◽  
Jessica Börstler ◽  
Hanna Jöst ◽  
Marlis Badusche ◽  
Daniel Desmecht ◽  
...  
Keyword(s):  
New Genus ◽  
Aedes Vexans ◽  
The Family

scholarly journals Characterization of a novel member of the family Caulimoviridae infecting Dioscorea nummularia in the Pacific, which may represent a new genus of dsDNA plant viruses

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0203038 ◽  
Author(s):  
Amit C. Sukal ◽  
Dawit B. Kidanemariam ◽  
James L. Dale ◽  
Robert M. Harding ◽  
Anthony P. James
Keyword(s):  
New Genus ◽  
Plant Viruses ◽  
The Pacific ◽  
The Family

Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae

2008 ◽  
Vol 153 (7) ◽  
pp. 1263-1270 ◽  
Author(s):  
Anna Maria Vaira ◽  
Clarissa J. Maroon-Lango ◽  
John Hammond
Keyword(s):  
Molecular Characterization ◽  
New Genus ◽  
Latent Virus ◽  
The Family

scholarly journals Full genome characterization of Laem Singh virus (LSNV) in shrimp Penaeus monodon

2020 ◽  
Author(s):  
Suparat Taengchaiyaphum ◽  
Jiraporn Srisala ◽  
Piyachat Sanguanrut ◽  
Chalermporn Ongvarrasopone ◽  
Timothy W. Flegel ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Plant Viruses ◽  
Virus Species ◽  
Full Genome ◽  
Cloning And Sequencing ◽  
Genome Characterization ◽  
Sequence Identity ◽  
The Family ◽  
Shrimp Virus

ABSTRACTLaem Singh virus (LSNV) was discovered in 2006 and proposed as a necessary but insufficient cause of retarded growth in the giant tiger shrimp Penaeus monodon. Its closest relatives were plant viruses including an unassigned Sobemovirus and viruses in the family Luteoviridae. During succeeding years, attempts to obtain the full LSNV genome sequence by genome walking failed. However, recent publication of the full sequence of Wenzhou shrimp virus 9 (WZSV 9) at GenBank revealed that LSNV sequences in our database shared 99% sequence identity with it. Thus, we hypothesized that LSNV and WZSV 9 were different isolates of the same virus species. Here we confirm that hypothesis by cloning and sequencing of the full genome of LSNV from P. monodon and by showing that it consists of two fragments each with 99% identity to the matching fragments of WZSV.

scholarly journals Genomic characterization of three novel Pseudomonas phages within the subfamily of Autographivirinae isolated from organic waste

2020 ◽  
Author(s):  
Jacob B Jørgensen ◽  
Amaru M Djuurhus ◽  
Alexander B. Carstens ◽  
Witold Kot ◽  
Cindy E. Morris ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Organic Waste ◽  
New Genus ◽  
Open Reading Frames ◽  
Genomic Comparison ◽  
Double Stranded Dna ◽  
The Family ◽  
Genomic Characterization ◽  
Reading Frames

AbstractThree phages targeting Pseudomonas syringae GAW0113 have been isolated from organic waste samples: Pseudomonas phage Bertil, Misse and Strit. The phages have double-stranded DNA genomes ranging from 41342 to 41374 bp in size comprising 50 to 51 open reading frames. The three phages genomes are highly similar and genomic comparison analyses shows that they all belong to the Autographivirinae subfamily of the family Podoviridae. The phages are however only distantly related to other members of this family, and have limited gene synteny with type-phages of other genera within Autographivirinae, suggesting that the newly isolated phages could represent a new genus.

Sign in / Sign up

Export Citation Format

Share Document