Varroa destructor is an effective vector of Israeli acute paralysis virus in the honeybee, Apis mellifera

Recent declines in honey bee (Apis mellifera L., 1758) populations worldwide have spurred significant research into the impact of pathogens on colony health. The role of the Israeli acute paralysis virus (IAPV) on hive mortality has become of particular concern since being correlated with colony losses. However, the molecular interactions between IAPV and its host remain largely unknown. To investigate changes in host protein expression during IAPV infection, mass-spectrometry-based quantitative proteomics was used to compare IAPV-infected and healthy pupae. Proteins whose expression levels changed significantly during infection were identified and functional analysis was performed to determine host systems and pathways perturbed by IAPV infection. Among the A. mellifera proteins most affected by IAPV, those involving translation and the ubiquitin–proteasome pathway were most highly enriched and future investigation of these pathways will be useful in identifying host proteins required for infection. This analysis represents an important first step towards understanding the honey bee host response to IAPV infection through the systems-level analysis of protein expression.

Download Full-text

Israeli acute paralysis virus not detected in Apis mellifera in New Zealand in a national survey

Journal of Apicultural Research ◽

10.3896/ibra.1.53.5.03 ◽

2014 ◽

Vol 53 (5) ◽

pp. 520-527 ◽

Cited By ~ 2

Author(s):

Andrew M J McFadden ◽

Kok-Mun Tham ◽

Mark Stevenson ◽

Mark Goodwin ◽

Howard Pharo ◽

...

Keyword(s):

New Zealand ◽

Apis Mellifera ◽

National Survey ◽

Israeli Acute Paralysis Virus ◽

Paralysis Virus

Download Full-text

Detection of Israeli Acute Paralysis Virus (IAPV) and Apis mellifera Filamentous Virus (AmFV) in Honey Bees in Mexico

Journal of Apicultural Science ◽

10.2478/jas-2018-0009 ◽

2018 ◽

Vol 62 (1) ◽

pp. 141-144 ◽

Cited By ~ 1

Author(s):

Mayra C. García-Anaya ◽

Alejandro Romo-Chacón ◽

Alma I. Sáenz-Mendoza ◽

Gerardo Pérez-Ordoñez ◽

Carlos H. Acosta-Muñiz

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Honey Bees ◽

Developmental Stages ◽

Viral Diseases ◽

Israeli Acute Paralysis Virus ◽

Colony Loss ◽

Polymerase Chain ◽

Bee Colonies ◽

Paralysis Virus

Abstract The recent alarming loss of honey bee colonies around the world is believed to be related to the presence of viruses. The aim of this study was to detect two major viral diseases, Apis mellifera Filamentous virus (AmFV) and Israeli Acute Paralysis Virus (IAPV) using Reverse Transcription - Polymerase Chain Reaction RT-PCR, in honey bees in Mexico. Adult and larvae honey bee samples were collected from asymptomatic colonies of six major beekeeping regions in the state of Chihuahua, Mexico. Both viruses were detected in both developmental stages of honey bees, IAPV at a higher prevalence (23.5%) as compared to AmFV, only in 0.9% of samples. However, this is the first report on AmFV infection in Mexican apiaries. Further studies are required to understand the AmFV and IAPV impact on colony loss in Mexico and to develop strategies for enhancing the control of viral diseases.

Download Full-text

In Vitro Infection of Pupae with Israeli Acute Paralysis Virus Suggests Disturbance of Transcriptional Homeostasis in Honey Bees (Apis mellifera)

PLoS ONE ◽

10.1371/journal.pone.0073429 ◽

2013 ◽

Vol 8 (9) ◽

pp. e73429 ◽

Cited By ~ 40

Author(s):

Humberto F. Boncristiani ◽

Jay D. Evans ◽

Yanping Chen ◽

Jeff Pettis ◽

Charles Murphy ◽

...

Keyword(s):

Apis Mellifera ◽

Honey Bees ◽

Israeli Acute Paralysis Virus ◽

Paralysis Virus

Download Full-text

First detection of Israeli acute paralysis virus (IAPV) in France, a dicistrovirus affecting honeybees (Apis mellifera)

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2008.07.006 ◽

2008 ◽

Vol 99 (3) ◽

pp. 348-350 ◽

Cited By ~ 46

Author(s):

Philippe Blanchard ◽

Frank Schurr ◽

Olivier Celle ◽

Nicolas Cougoule ◽

Patrick Drajnudel ◽

...

Keyword(s):

Apis Mellifera ◽

Israeli Acute Paralysis Virus ◽

Paralysis Virus

Download Full-text

Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera, Hymenoptera: Apidae)

PLoS Pathogens ◽

10.1371/journal.ppat.1001160 ◽

2010 ◽

Vol 6 (12) ◽

pp. e1001160 ◽

Cited By ~ 139

Author(s):

Wayne Hunter ◽

James Ellis ◽

Dennis vanEngelsdorp ◽

Jerry Hayes ◽

Dave Westervelt ◽

...

Keyword(s):

Apis Mellifera ◽

Honey Bees ◽

Large Scale ◽

Virus Disease ◽

Field Application ◽

Israeli Acute Paralysis Virus ◽

Rnai Technology ◽

Scale Field ◽

Large Scale Field ◽

Paralysis Virus

Download Full-text

УГРОЗЫ РАСПРОСТРАНЕНИЯ ВИРУСНЫХ ИНФЕКЦИЙ У ПЧЕЛ (Apis mellifera L.) И РОЛЬ КЛЕЩА Varroa destructor В РАЗВИТИИ ПАТОЛОГИЙ

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2016.2.156rus ◽

2016 ◽

Vol 51 (2) ◽

pp. 156-171

Author(s):

А.В. СПРЫГИН ◽

◽

Ю.Ю. БАБИН ◽

Е.М. ХАНБЕКОВА ◽

Л.Е. РУБЦОВА ◽

...

Keyword(s):

Apis Mellifera ◽

Varroa Destructor

Download Full-text

Eficacia del humo de frutos de Guazuma ulmifolia (Sterculiaceae) y vapores de timol para el control de Varroa destructor infestando abejas africanizadas

Revista Mexicana de Ciencias Pecuarias ◽

10.22319/rmcp.v10i3.4810 ◽

2019 ◽

Vol 10 (3) ◽

pp. 778-788

Author(s):

William De Jesús May-Itzá ◽

Luis Abdelmir Abdelmir Medina Medina

Keyword(s):

Apis Mellifera ◽

Varroa Destructor ◽

Guazuma Ulmifolia

Se evaluó la eficacia del humo de los frutos secos de Guazuma ulmifolia y los vapores de timol en el control del ácaro Varroa destructor infestando colonias de abejas africanizadas (Apis mellifera) de Yucatán. Se utilizaron tres tratamientos: Grupo 1 (G1), las colonias de abejas recibieron 5 a 8 bocanadas de humo de los frutos secos de G. ulmifolia dos veces por semana, durante un período de tres semanas; Grupo 2 (G2), las colonias recibieron 4-8 g de cristales de timol con tres aplicaciones cada siete días, y Grupo 3 (G3 o grupo control) las colonias no recibieron ningún tratamiento durante las tres semanas del experimento. Se colectaron 200 a 300 abejas adultas de cada colonia previo a la aplicación de los tratamientos (día 0) y a los 7, 14 y 21 días después de las aplicaciones, con la finalidad de determinar los niveles de infestación y eficacia de los tratamientos. Los resultados indican que los niveles de infestación de V. destructor en las abejas adultas disminuyeron al final del experimento (21 días) y fueron estadísticamente diferentes para los tres tratamientos, siendo menor para G2. La eficacia al final de los tratamientos fue de 41 y 69 %, para G1 y G2, respectivamente. Estos resultados corroboran que la aplicación de cristales de timol es una alternativa para el control del ácaro V. destructor en Yucatán, y que la aplicación del humo de los frutos secos de G. ulmifolia reduce los niveles de infestación de este parásito en comparación con las colonias que no recibieron ningún tipo de tratamiento (G3).

Download Full-text

Exploring Two Honey Bee Traits for Improving Resistance Against Varroa destructor: Development and Genetic Evaluation

Insects ◽

10.3390/insects12030216 ◽

2021 ◽

Vol 12 (3) ◽

pp. 216

Author(s):

Matthieu Guichard ◽

Benoît Droz ◽

Evert W. Brascamp ◽

Adrien von Virag ◽

Markus Neuditschko ◽

...

Keyword(s):

Apis Mellifera ◽

Honey Bee ◽

Honey Bees ◽

Varroa Destructor ◽

Field Trial ◽

Genetic Evaluation ◽

Phenotypic Correlation ◽

Apis Mellifera Mellifera ◽

Novel Traits ◽

Resistance Traits

For the development of novel selection traits in honey bees, applicability under field conditions is crucial. We thus evaluated two novel traits intended to provide resistance against the ectoparasitic mite Varroa destructor and to allow for their straightforward implementation in honey bee selection. These traits are new field estimates of already-described colony traits: brood recapping rate (‘Recapping’) and solidness (‘Solidness’). ‘Recapping’ refers to a specific worker characteristic wherein they reseal a capped and partly opened cell containing a pupa, whilst ‘Solidness’ assesses the percentage of capped brood in a predefined area. According to the literature and beekeepers’ experiences, a higher recapping rate and higher solidness could be related to resistance to V. destructor. During a four-year field trial in Switzerland, the two resistance traits were assessed in a total of 121 colonies of Apis mellifera mellifera. We estimated the repeatability and the heritability of the two traits and determined their phenotypic correlations with commonly applied selection traits, including other putative resistance traits. Both traits showed low repeatability between different measurements within each year. ‘Recapping’ had a low heritability (h2 = 0.04 to 0.05, depending on the selected model) and a negative phenotypic correlation to non-removal of pin-killed brood (r = −0.23). The heritability of ‘Solidness’ was moderate (h2 = 0.24 to 0.25) and did not significantly correlate with resistance traits. The two traits did not show an association with V. destructor infestation levels. Further research is needed to confirm the results, as only a small number of colonies was evaluated.

Download Full-text

Comparing Survival of Israeli Acute Paralysis Virus Infection among Stocks of U.S. Honey Bees

Insects ◽

10.3390/insects12010060 ◽

2021 ◽

Vol 12 (1) ◽

pp. 60

Author(s):

Shilpi Bhatia ◽

Saman S. Baral ◽

Carlos Vega Melendez ◽

Esmaeil Amiri ◽

Olav Rueppell

Keyword(s):

Honey Bee ◽

Honey Bees ◽

Virus Infections ◽

Israeli Acute Paralysis Virus ◽

Immune Genes ◽

Starting Point ◽

Bee Health ◽

Honey Bee Health ◽

Survival Differences ◽

Paralysis Virus

Among numerous viruses that infect honey bees (Apis mellifera), Israeli acute paralysis virus (IAPV) can be linked to severe honey bee health problems. Breeding for virus resistance may improve honey bee health. To evaluate the potential for this approach, we compared the survival of IAPV infection among stocks from the U.S. We complemented the survival analysis with a survey of existing viruses in these stocks and assessing constitutive and induced expression of immune genes. Worker offspring from selected queens in a common apiary were inoculated with IAPV by topical applications after emergence to assess subsequent survival. Differences among stocks were small compared to variation within stocks, indicating the potential for improving honey bee survival of virus infections in all stocks. A positive relation between worker survival and virus load among stocks further suggested that honey bees may be able to adapt to better cope with viruses, while our molecular studies indicate that toll-6 may be related to survival differences among virus-infected worker bees. Together, these findings highlight the importance of viruses in queen breeding operations and provide a promising starting point for the quest to improve honey bee health by selectively breeding stock to be better able to survive virus infections.

Download Full-text