scholarly journals Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera, Hymenoptera: Apidae)

2010 ◽  
Vol 6 (12) ◽  
pp. e1001160 ◽  
Author(s):  
Wayne Hunter ◽  
James Ellis ◽  
Dennis vanEngelsdorp ◽  
Jerry Hayes ◽  
Dave Westervelt ◽  
...  
2010 ◽  
Vol 108-111 ◽  
pp. 1158-1163 ◽  
Author(s):  
Peng Cheng Nie ◽  
Di Wu ◽  
Weiong Zhang ◽  
Yan Yang ◽  
Yong He

In order to improve the information management of the modern digital agriculture, combined several modern digital agriculture technologies, namely wireless sensor network (WSN), global positioning system (GPS), geographic information system (GIS) and general packet radio service (GPRS), and applied them to the information collection and intelligent control process of the modern digital agriculture. Combining the advantage of the local multi-channel information collection and the low-power wireless transmission of WSN, the stable and low cost long-distance communication and data transmission ability of GPRS, the high-precision positioning technology of the DGPS positioning and the large-scale field information layer-management technology of GIS, such a hybrid technology combination is applied to the large-scale field information and intelligent management. In this study, wireless sensor network routing nodes are disposed in the sub-area of field. These nodes have GPS receiver modules and the electric control mechanism, and are relative positioned by GPS. They can real-time monitor the field information and control the equipment for the field application. When the GPS position information and other collected field information are measured, the information can be remotely transmitted to PC by GPRS. Then PC can upload the information to the GIS management software. All the field information can be classified into different layers in GIS and shown on the GIS map based on their GPS position. Moreover, we have developed remote control software based on GIS. It can send the control commands through GPRS to the nodes which have control modules; and then we can real-time manage and control the field application. In conclusion, the unattended automatic wireless intelligent technology for the field information collection and control can effectively utilize hardware resources, improve the field information intelligent management and reduce the information and intelligent cost.


2018 ◽  
Vol 62 (1) ◽  
pp. 141-144 ◽  
Author(s):  
Mayra C. García-Anaya ◽  
Alejandro Romo-Chacón ◽  
Alma I. Sáenz-Mendoza ◽  
Gerardo Pérez-Ordoñez ◽  
Carlos H. Acosta-Muñiz

Abstract The recent alarming loss of honey bee colonies around the world is believed to be related to the presence of viruses. The aim of this study was to detect two major viral diseases, Apis mellifera Filamentous virus (AmFV) and Israeli Acute Paralysis Virus (IAPV) using Reverse Transcription - Polymerase Chain Reaction RT-PCR, in honey bees in Mexico. Adult and larvae honey bee samples were collected from asymptomatic colonies of six major beekeeping regions in the state of Chihuahua, Mexico. Both viruses were detected in both developmental stages of honey bees, IAPV at a higher prevalence (23.5%) as compared to AmFV, only in 0.9% of samples. However, this is the first report on AmFV infection in Mexican apiaries. Further studies are required to understand the AmFV and IAPV impact on colony loss in Mexico and to develop strategies for enhancing the control of viral diseases.


2018 ◽  
Author(s):  
Mohammad Al-Ghnemi ◽  
Erdal Ozkan ◽  
Mohammed Al-Murayri ◽  
Malcolm Pitts ◽  
Elio Dean

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73429 ◽  
Author(s):  
Humberto F. Boncristiani ◽  
Jay D. Evans ◽  
Yanping Chen ◽  
Jeff Pettis ◽  
Charles Murphy ◽  
...  

1979 ◽  
Vol 31 (06) ◽  
pp. 690-696
Author(s):  
J.C. Howell ◽  
R.W. McAtee ◽  
W.O. Snyder ◽  
K.L. Tonso

2010 ◽  
Author(s):  
Julia Levashina ◽  
Frederick P. Morgeson ◽  
Michael A. Campion

Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 60
Author(s):  
Shilpi Bhatia ◽  
Saman S. Baral ◽  
Carlos Vega Melendez ◽  
Esmaeil Amiri ◽  
Olav Rueppell

Among numerous viruses that infect honey bees (Apis mellifera), Israeli acute paralysis virus (IAPV) can be linked to severe honey bee health problems. Breeding for virus resistance may improve honey bee health. To evaluate the potential for this approach, we compared the survival of IAPV infection among stocks from the U.S. We complemented the survival analysis with a survey of existing viruses in these stocks and assessing constitutive and induced expression of immune genes. Worker offspring from selected queens in a common apiary were inoculated with IAPV by topical applications after emergence to assess subsequent survival. Differences among stocks were small compared to variation within stocks, indicating the potential for improving honey bee survival of virus infections in all stocks. A positive relation between worker survival and virus load among stocks further suggested that honey bees may be able to adapt to better cope with viruses, while our molecular studies indicate that toll-6 may be related to survival differences among virus-infected worker bees. Together, these findings highlight the importance of viruses in queen breeding operations and provide a promising starting point for the quest to improve honey bee health by selectively breeding stock to be better able to survive virus infections.


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