scholarly journals Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae

2003 ◽  
Vol 84 (12) ◽  
pp. 3459-3464 ◽  
Author(s):  
Livia Stavolone ◽  
Antonio Ragozzino ◽  
Thomas Hohn
Keyword(s):  
Genomic Sequence ◽  
Infected Plant ◽  
Virus Genome ◽  
Mosaic Disease ◽  
Open Reading Frames ◽  
Primer Binding Site ◽  
Yellow Leaf ◽  
Leaf Curling ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member of the Caulimoviridae family. All characteristic domains conserved in plant pararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.

scholarly journals Genes Ia, II, III, IV and V of Soybean chlorotic mottle virus are essential but the gene Ib product is non-essential for systemic infection

2001 ◽  
Vol 82 (6) ◽  
pp. 1481-1489 ◽  
Author(s):  
Yutaka Takemoto ◽  
Tadaaki Hibi
Keyword(s):  
Mutational Analysis ◽  
Systemic Infection ◽  
Peptide Sequencing ◽  
Cauliflower Mosaic Virus ◽  
Open Reading Frames ◽  
Mottle Virus ◽  
Primer Binding Site ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Infected Cells

Soybean chlorotic mottle virus (SbCMV) is the type species of the genus ‘Soybean chlorotic mottle-like viruses’, within the family Caulimoviridae. The double-stranded DNA genome of SbCMV (8178 bp) contains eight major open reading frames (ORFs). Viral genes essential and non-essential for the replication and movement of SbCMV were investigated by mutational analysis of an infectious 1·3-mer DNA clone. The results indicated that ORFs Ia, II, III, IV and V were essential for systemic infection. The product of ORF Ib was non-essential, although the putative tRNAMet primer-binding site in ORF Ib was proved to be essential. Immunoselection PCR revealed that an ORF Ia deletion mutant was encapsidated in primarily infected cells, indicating that ORF Ia is required for virus movement but not for replication. ORF IV was confirmed to encode a capsid protein by peptide sequencing of the capsid. Analysis of the viral transcripts showed that the SbCMV DNA genome gives rise to a pregenomic RNA and an ORF VI mRNA, as shown in the case of Cauliflower mosaic virus.

scholarly journals Novel targets for engineering Physostegia chlorotic mottle and tomato brown rugose fruit virus-resistant tomatoes: in silico prediction of tomato microRNA targets

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10096
Author(s):  
Yahya Zakaria Abdou Gaafar ◽  
Heiko Ziebell
Keyword(s):  
In Silico ◽  
Viral Infections ◽  
Control Strategies ◽  
Plant Viruses ◽  
Open Reading Frames ◽  
Tomato Production ◽  
Tomato Plants ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Tomato Microrna

Background Physostegia chlorotic mottle virus (PhCMoV; genus: Alphanucleorhabdovirus, family: Rhabdoviridae) and tomato brown rugose fruit virus (ToBRFV; genus: Tobamovirus, family: Virgaviridae) are newly emerging plant viruses that have a dramatic effect on tomato production. Among various known virus-control strategies, RNAi-mediated defence has shown the potential to protect plants against various pathogens including viral infections. Micro(mi)RNAs play a major role in RNAi-mediated defence. Methods Using in silico analyses, we investigated the possibility of tomato-encoded miRNAs (TomiRNA) to target PhCMoV and ToBRFV genomes using five different algorithms, i.e., miRanda, RNAhybrid, RNA22, Tapirhybrid and psRNATarget. Results The results revealed that 14 loci on PhCMoV and 10 loci on ToBRFV can be targeted by the TomiRNAs based on the prediction of at least three algorithms. Interestingly, one TomiRNA, miR6026, can target open reading frames from both viruses, i.e., the phosphoprotein encoding gene of PhCMoV, and the two replicase components of ToBRFV. There are currently no commercially available PhCMoV- or ToBRFV-resistant tomato varieties, therefore the predicted data provide useful information for the development of PhCMoV- and ToBFRV-resistant tomato plants.

scholarly journals Characterization of a New Nepovirus Causing a Leaf Mottling Disease in Petunia hybrida

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1017-1021 ◽  
Author(s):  
Sara Bratsch ◽  
Benham Lockhart ◽  
Dimitre Mollov
Keyword(s):  
Petunia Hybrida ◽  
High Throughput Sequencing ◽  
Genomic Sequence ◽  
Phylogenetic Analyses ◽  
Pcr Amplification ◽  
Particle Morphology ◽  
Reverse Transcription Pcr ◽  
Full Genomic Sequence ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Icosahedral virus-like particles were isolated from Petunia hybrida cuttings with interveinal chlorotic mottling. The virus was transmitted by mechanical inoculation from infected to healthy P. hybrida, and was found to contain a bipartite RNA genome of 7.6 and 3.8 kilobases. Full genomic sequence was obtained by high-throughput sequencing combined with RACE amplification of the 5′-termini of RNAs 1 and 2, and reverse-transcription PCR amplification of the 3′-termini with oligo-dT and sequence specific primers. Based on particle morphology, genome organization, and phylogenetic analyses, it was concluded that the new virus is a member of the genus Nepovirus, subgroup A. This new virus causing a leaf mottling disease of petunia was provisionally named Petunia chlorotic mottle virus (PCMoV).

scholarly journals Tomato Chlorotic Mottle Virus Is a Target of RNA Silencing but the Presence of Specific Short Interfering RNAs Does Not Guarantee Resistance in Transgenic Plants

2006 ◽  
Vol 81 (4) ◽  
pp. 1563-1573 ◽  
Author(s):  
Simone G. Ribeiro ◽  
Hendrikus Lohuis ◽  
Rob Goldbach ◽  
Marcel Prins
Keyword(s):  
Rna Silencing ◽  
Virus Genome ◽  
Mottle Virus ◽  
Small Interfering Rnas ◽  
Dna Viruses ◽  
Hairpin Construct ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Transgenic Lines ◽  
Mere Presence

ABSTRACT Tomato chlorotic mottle virus (ToCMoV) is a begomovirus found widespread in tomato fields in Brazil. ToCMoV isolate BA-Se1 (ToCMoV-[BA-Se1]) was shown to trigger the plant RNA silencing surveillance in different host plants and, coinciding with a decrease in viral DNA levels, small interfering RNAs (siRNAs) specific to ToCMoV-[BA-Se1] accumulated in infected plants. Although not homogeneously distributed, the siRNA population in both infected Nicotiana benthamiana and tomato plants represented the entire DNA-A and DNA-B genomes. We determined that in N. benthamiana, the primary targets corresponded to the 5′ end of AC1 and the embedded AC4, the intergenic region and 5′ end of AV1 and overlapping central part of AC5. Subsequently, transgenic N. benthamiana plants were generated that were preprogrammed to express double-stranded RNA corresponding to this most targeted portion of the virus genome by using an intron-hairpin construct. These plants were shown to indeed produce ToCMoV-specific siRNAs. When challenge inoculated, most transgenic lines showed significant delays in symptom development, and two lines had immune plants. Interestingly, the levels of transgene-produced siRNAs were similar in resistant and susceptible siblings of the same line. This indicates that, in contrast to RNA viruses, the mere presence of transgene siRNAs corresponding to DNA virus sequences does not guarantee virus resistance and that other factors may play a role in determining RNA-mediated resistance to DNA viruses.

scholarly journals Identification and Properties of a Carlavirus Causing Chlorotic Mottle of Florists' Hydrangea (H. macrophylla) in the United States

Plant Disease ◽  
2009 ◽  
Vol 93 (9) ◽  
pp. 891-895 ◽  
Author(s):  
Jose Ernesto Machado Caballero ◽  
Ben E. Lockhart ◽  
Shauna L. Mason ◽  
Margery Daughtrey
Keyword(s):  
United States ◽  
The United States ◽  
Open Reading Frames ◽  
Serological Tests ◽  
Hydrangea Macrophylla ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Leaf Deformation ◽  
Chlorotic Mottling ◽  
Hydrangea Ringspot Virus

A previously uncharacterized virus with flexuous filamentous particles 660 nm in length was identified in the United States in florists' hydrangea (Hydrangea macrophylla), in which it caused chlorotic mottling, leaf deformation, and discoloration. The virus, tentatively named Hydrangea chlorotic mottle virus (HdCMV), was transmitted readily by mechanical inoculation and by Myzus persicae, but infected only H. macrophylla. The amino acid sequence of a 1.7-kb amplicon comprising the 3′ terminus of the HdCMV genome contained one partial and three complete putative open reading frames (ORFs) most similar in size, arrangement, and sequence to the homologous regions of the genomes of known carlaviruses. Based on virion morphology, genome properties, and current criteria for species demarcation, it was concluded that HdCMV represented a new species in the genus Carlavirus. Hydrangea ringspot virus (HdRSV, genus Potexvirus) occurred in mixed infections with HdCMV, but the two viruses could be distinguished readily by serological tests.

scholarly journals Cereal Chlorotic Mottle Virus?Purification, Serology and Electron Microscopy in Plant and Insect Tissues

1979 ◽  
Vol 32 (3) ◽  
pp. 399 ◽  
Author(s):  
RS Greber ◽  
DH Gowanfock
Keyword(s):  
Infected Plant ◽  
Mottle Virus ◽  
Intracellular Location ◽  
Virus Particles ◽  
Perinuclear Space ◽  
Cytoplasmic Vesicles ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Similar Appearance ◽  
Granular Matrix

Cereal chlorotic mottle virus (CCMV) was shown to accumulate in the perinuclear space and cytoplasmic vesicles of infected plant cells. The virus was present in both vascular and mesophyll tissues. In infected leafhoppers Nesoclutha pa zlida (Evans) the virus particles had a similar appearance and intracellular location but sometimes appeared to be in a granular matrix and were dispersed throughout some degenerated nucleii.

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